AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activit...AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.展开更多
Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to invest...Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.展开更多
Lentinula edodes is the second largest edible mushroom in the world and is widely used as food and medicine.Modern research shows that lentinan(LNT)is the main active component of L.edodes.It has anti-cancer,treatment...Lentinula edodes is the second largest edible mushroom in the world and is widely used as food and medicine.Modern research shows that lentinan(LNT)is the main active component of L.edodes.It has anti-cancer,treatment of diabetes,intestinal protection,anti-inflammatory,anti-oxidation,anti-aging,hepatoprotective,immune-regulating effects.In this review,the biological activity,action mechanism and structure-activity relationship of LNT in recent years are reviewed.On this basis,the existing problems were discussed,and the future research and application of LNT were prospected.Finally,it is hoped that this review will promote the in-depth study of LNT and provide a reference for its development as a drug and functional food.展开更多
基金funded by the open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the science and technology projects in Guangzhou(202201020632,202201020638,202206010100,202201020655 and 202102010202)+2 种基金the Guangdong Natural Science Foundation(2020A1515110648)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)The Guangzhou Medical University Students’Science and Technology Innovation Project(02-408-2203-2079,2021AEK119).
文摘AH_(2)O_(2)-induced oxidative stress injury cell model was established to investigate the antioxidant effect of nanoselenium on mouse spermatocyte lines and the regulation mechanism of the expression level and activity of seleniumcontaining antioxidant enzymes induced by oxidative stress.A safe and effective nano-drug system of functionalized selenium-containing nanoparticles(SeNPs)was developed with lentinan(LNT)(SeNPs@LNT).Mice spermatocyte line GC2-spg cells were treated with SeNPs@LNT(1,2,4,8,16,32μM)for 24-72 h to evaluate the cytotoxicity of selenium.GC2-spg cells were randomly divided into the following groups:control,hydrogen peroxide(H_(2)O_(2)),SeNPs@LNT,and H_(2)O_(2)+SeNPs@LNT groups.H_(2)O_(2)+SeNPs@LNT group was pretreated with SeNPs@LNT 4μM for 12 h,followed by H_(2)O_(2)600μM for 8 h.The cell viability decreased in the H_(2)O_(2) group and increased significantly in the SeNPs@LNT group.Compared with the H_(2)O_(2) group,the SeNPs@LNT+H_(2)O_(2) group exhibited obvious red fluorescence,indicating a higher level of mitochondrial membrane potential.The content of intracellular reactive oxygen species(ROS)in the SeNPs@LNT group reduced significantly,and the intensity of green fluorescence in the SeNPs@LNT+H_(2)O_(2) group decreased significantly compared with the H_(2)O_(2) group,indicating the inhibitory effect of SeNPs@LNT on the generation of ROS-induced oxidative stress.The activity of GPx and SOD increased significantly in the SeNPs@LNT group.The expression of p53 decreased significantly under the intervention of nano-selenium,and GPx1 expression increased.In the oxidative stress group,the expressions of DNA damage-related proteins and apoptosis-related proteins were higher than those in other groups.Thus,SeNPs@LNT can promote GC2-spg cell proliferation,improve GPx and SOD activities,remove intracellular ROS,and reduce mitochondrial damage and functional abnormalities caused by oxidative stress by regulating the ERK and p53 protein levels.SeNPs@LNT has good biological activity and antioxidant effect,which can be used to protect the male reproductive system from oxidative stress.
基金Science and Technology Projects in Guangzhou(202201020632,202201020638,202206010100,202201020655,and 202102010202)the Guangdong Natural Science Foundation(2020A1515110648)+2 种基金the Open Project of Guangdong Key Laboratory of Marine Materia(LMM2020-7)the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications(2020A03)the Guangzhou Medical University Students’Science and Technology Innovation Project(2021AEK119 and 02-408-2203-2079).
文摘Background:Nano-selenium has been widely used in antiviral and anticancer therapy,and has the advantages of good targeting and low toxicity.For the first time,we combined male reproduction with nano-selenium to investigate its antioxidant effect.This study investigated the protective effect of lentinan functionalized selenium nanoparticles on oxidative stress injury of the hydrogen peroxide(H_(2)O_(2))-induced Leydig cell line,TM3.Methods:The suitable concentration of nano-selenium treatment to promote cell proliferation was also discussed.The concentration of 4μM could significantly promote the growth of TM3 cells.Oxidative stress damage was caused using an 800μM concentration of hydrogen peroxide.The cells were divided into four groups:normal control group,oxidative stress treatment group,H_(2)O_(2)+SeNPs@LNT group,and SeNPs@LNT group.The H_(2)O_(2)+SeNPs@LNT group was pretreated with 4μM of SeNPs@LNT for 12 h,followed by 800μM of H_(2)O_(2)for 8 h.Results:Nano-selenium could significantly promote the proliferation and viability of TM3 cells.SeNPs@LNT treatment increased the level of mitochondrial membrane potential in normal cells and slowed down the decline in mitochondrial membrane potential level caused by oxidative stress injury.In addition,the increase in reactive oxygen species caused by oxidative stress was inhibited by SeNPs@LNT treatment.The apoptosis of TM3 cells was detected,and SeNPs@LNT alleviated the necrosis and apoptosis of TM3 cells induced by H_(2)O_(2).Nano-selenium plays a protective role against oxidative H_(2)O_(2)-induced stress injury in TM3 cells through the changes in the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and P53 pathway,and the expression levels of other related proteins,protein kinase B(AKT)and C3.Conclusion:SeNPs@LNT exhibited good biological activity and antioxidant effect and can thus be used to protect the male reproductive system from oxidative stress.
基金Supported by National Natural Science Foundation of China (82360716).
文摘Lentinula edodes is the second largest edible mushroom in the world and is widely used as food and medicine.Modern research shows that lentinan(LNT)is the main active component of L.edodes.It has anti-cancer,treatment of diabetes,intestinal protection,anti-inflammatory,anti-oxidation,anti-aging,hepatoprotective,immune-regulating effects.In this review,the biological activity,action mechanism and structure-activity relationship of LNT in recent years are reviewed.On this basis,the existing problems were discussed,and the future research and application of LNT were prospected.Finally,it is hoped that this review will promote the in-depth study of LNT and provide a reference for its development as a drug and functional food.
