A high-efficiency somatic embryogenesis protocol of Japanese larch （Larix leptolepis Gordon） has been established in our investigation. Calli were induced from immature zygotic embryos of female cones ofL. leptolepi...A high-efficiency somatic embryogenesis protocol of Japanese larch （Larix leptolepis Gordon） has been established in our investigation. Calli were induced from immature zygotic embryos of female cones ofL. leptolepis and then subcultured regularly on to a modified Gupta and Durzan （DCR） basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur- ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid （ABA） com- pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media展开更多
Experiments on spaying Penzhangjing on seedlings ofLarix leptolepis was conducted for a year. The height growth and diameter at foot of experimental seedlings increased by 34.3% and 35.5%, respectively, compared with ...Experiments on spaying Penzhangjing on seedlings ofLarix leptolepis was conducted for a year. The height growth and diameter at foot of experimental seedlings increased by 34.3% and 35.5%, respectively, compared with control seedlings. Variance analysis concludes that Penzhangjing have obvious effect on growth of height and diameter at foot of seedlings.展开更多
基金the National Natural Science Foundation of China （Grant No. 30471366） the National Science Fund of China for Distinguished Young Scholars （30225005）.
文摘A high-efficiency somatic embryogenesis protocol of Japanese larch （Larix leptolepis Gordon） has been established in our investigation. Calli were induced from immature zygotic embryos of female cones ofL. leptolepis and then subcultured regularly on to a modified Gupta and Durzan （DCR） basal medium for 5 years. Embryogenic tissues showed distinct morphological changes dur- ing somatic embryo development when they were transferred to a maturation medium supplemented with abscisic acid （ABA） com- pared with the morphology in a medium lacking ABA. Histological observations indicated that polyembryony was a characteristic feature during early embryogeny and somatic embryos at later stages showed normal histodifferentiation. In addition, histochemical analysis revealed that abundant starch granules and proteins accumulated in mature embryos, indicating that they played important roles in the development and regeneration of normal plantlets from somatic embryos on hormone-free germination media
文摘Experiments on spaying Penzhangjing on seedlings ofLarix leptolepis was conducted for a year. The height growth and diameter at foot of experimental seedlings increased by 34.3% and 35.5%, respectively, compared with control seedlings. Variance analysis concludes that Penzhangjing have obvious effect on growth of height and diameter at foot of seedlings.
