ISSR Analysis on Genetic Diversity of the 34 Populations of Oryza meyeriana Distributing in Yunnan Province,China

The genetic diversity of the 34 populations of wild rice Oryza meyeriana Baill. distributed in Yunnan Province, China was analyzed using 13 inter-simple sequence repeat （ISSR） markers. A total of 168 bands were amplified, of which 135 polymorphic bands were discovered and the percentage of polymorphic bands （PPB） was 80.36%. A genetic diversity was revealed as Nei＇s gene diversity （H） = 0.2666 and Shannon information index （I） = 0.4028 at population level. The 34 populations were divided into different groups based on administrative regions, latitude and longitudes, river areas, altitudes of their origins, and their indexes such as Na （number of alleles）, Ne （effective number of alleles）, H, I and PPB were calculated. Richer genetic diversity was found in the wild rice populations distributed in Simao Prefecture than that in Lingcang Prefecture or Xishuangbanna Prefecture whereas the least genetic diversity was in Baoshan Prefecture or Dehong Prefecture. Rich genetic diversity was also discovered in the wild rice populations originated from higher than 710 m altitude around the middle and lower reaches of the Lancang River belonging to the Pacific Ocean drainage system. The 34 populations could be classified into two groups, one group covered the wild rice distributing in Simao Prefecture only while the other group covered ones in Lingcang, Xishuangbanna and Dehong Prefectures. The issue on how to effectively conserve the wild rice germplasm was discussed.

Minority population and sustainable development in mountain regions a case study of the Tibetan inhabited region in Sichuan, China

China boasts 56 nationalities who are mostly inhabited in the sparsely populated mountain regions in West China. Like many other places in the Han nationality distributed regions, minority nationality inhabited regions are also facing the challenge of sustainable development in terms of both natural and human factors. Taking the Tibetan inhabited regions (TIR) in Sichuan Province as a case, this paper points out some major problems in the process of economic development in perspective of sustainability and leaves some hints for development strategies. While natural conditions such as geological instability are playing a major role to the environmental degradation, human activities and improper use of resources might constitute an important agent in the environmental deterioration. In order to promote local economic development and improve people’s living standard, sustainable planning is expected to be made and people’s awareness of sustainability should be strengthened.

Population Structure Dynamics and Control Countermeasures of Sugarcane Borers in Yunnan Sugarcane Area

To control sugarcane borers scientifically and effectively, the species, population structure and dynamics of sugarcane borers in Yunnan sugarcane areas were analyzed, based on the historical data and survey research. The distribution area and damage of the major sugarcane borers in Yunnan sugarcane areas were ascertained, and the factors influencing population structure dynamics of sugarcane borers and their rampant causes were revealed. According to the damaging characteristics of sugarcane borers, suitable control countermeasures were proposed, including establish-ing and improving sugarcane quarantine system, enhancing quarantine of imported varieties/clones, investigating new sugarcane borer populations thoroughly and systematically, establishing early warning and monitoring system, actively carrying out physical and biological control, and enhancing protection of natural enemies. These countermeasures could help to effectively control sugarcane borers and reduce the damage caused by these borers.

Genetic differentiations between randomly and selectively bred pig populations in Yunnan,China

To assess the genetic diversity between randomly and selectively bred populations,we sequenced 438 bp of the mitochondrial DNA control region from 102 pigs.These samples represented four native pig breeds,one nucleus and one conservation herd from Yunnan,China.Twenty haplotypes with sixteen polymorphic sites were identified.The number of haplotypes in the nucleus herd of Saba pig and the conservation herd of Banna miniature pig were restricted to three and one,respectively,while the randomly bred pig populations exhibited over six haplotypes.Notably,haplotype diversity in randomly bred populations was significantly greater than the selectively bred populations（h=0.732 vs.0.425 and 0,exact test,P≤0.0036）.These findings demonstrate that selective breeding generated low genetic diversity compared to randomly bred pig breeds.A timely intervention and well programmed breeding approach would stop further genetic diversity reduction in the nucleus and conservation herds of native pig breeds.Otherwise,selective breeding would dramatically reduce genetic diversity in only several years,indicating that sharp contradictions exist between breeding,conservation and genetic diversity.Genetic relationships are discussed based on net genetic distances among pig populations.

Building a Comprehensive Well-off Society in Yunnan＇s Minority Areas A Study of the Prediction on the Differences in Life Quality Indexes

Based on the gray theory and GM （1, 1） model, the life quality indexes in next few years are predicted using the statistical data of the life quality indexes of Yunnan＇ s 8 minority areas in recent years and also compared with the standard value （2020） of building the national well-off society, and the differences between them are measured and calculated. Thus, effective reference information is provided for the decision-making of government.

Genetic polymorphism of mitochondrial DNA HVS-I and HVS-II of Chinese Tu ethnic minority group

We analyzed the two hypervariable segments HVS-I and HVS-II of 108 Chinese Tu ethnic minority group samples for forensic and population genetics purposes, Comparing with Anderson sequence, 79 polymorphic loci in HVS-I and 40 in HVS-II were found in Chinese Tu ethnic minority group mtDNA sequences, and 90 and 64 haplotypes were then defined. Haplotype diversity and the mean pairwise differences were 0.9903±0.0013 and 5.7785 in HVS-I, and 0.9777±0.0013 and 3.5819 in HVS-II, respectively. By analyzing the hypervariable domain from nucleotide 1,6180 to 1,6193 in HVS-I, we defined some new types of sequence variations. We also compared the relationship between Tu population and other populations using mtDNA HVS-I sequences. According to Rst genetic distances, the phylogenetic tree showed that the Tu population, the Xi＇an Han population, the Chinese Korean, and the Mongol ethnic group were in a clade. This indicated a close genetic relationship between them. There were far relations between the Tu population and other Chinese southern Han populations, Siberian, European, African, and other foreign populations. The results suggest that Tu population has a multi-origin and has also merged with other local populations.

Migration routes,population status and important sites used by the globally threatened Black-faced Spoonbill(Platalea minor):a synthesis of surveys and tracking studies

Background:The Black-faced Spoonbill(Platalea minor)is a globally threatened species,nesting mainly in western Korea with smaller numbers breeding in Liaoning Province,China,and Far East Russia.Recent winter field surveys to estimate the species'population size were almost totally conducted in coastal areas,but tracking studies showed that some individuals now winter inland.To ensure its long-term survival,we need a more comprehensive assessment of the current distribution and abundance of the species.Methods:We combined the most recent count data and satellite tracking information to update existing informa-tion about the population abundance and distribution of the Black-faced Spoonbill at all stages of its annual life cycle,and how these have changed during 2004-2020.Results:Black-faced Spoonbills mainly breed on the west coast of the Korean peninsula,while immature birds show a wider summer distribution throughout Yellow Sea coastal areas,when a few remain on wintering sites in the south.Combined tracking results and mid-winter counts confirmed known wintering sites on the east and south coasts of China,but showed that the species also winters on wetlands in the Yangtze River floodplain and in Southeast Asia.During 2004-2020,counts of wintering birds in coastal habitats increased from 1198 to 4864,with numbers wintering on the island of Taiwan contributing most to the overall increase.Latest counts found 5222 in 2021.We also identify key wintering and stopover sites as well as their current conservation status.Conclusions:This study revised the known summering and wintering ranges of the Black-faced Spoonbill and assessed the conservation status of key sites based on a combination of field survey and satellite tracking data.We recommend prioritisation of further field research to identify and survey inland wintering areas in the Yangtze River floodplain and summering areas of immature birds.More tracking of adult individuals and birds during spring migration is necessary to fill these information gaps.We also suggest establishing a Black-faced Spoonbill monitoring platform to store,share and show real-time distribution range and population abundance data.

The trend of low fertility and aging in ethnic minority areas and the policy responses in China:An analysis based on data from the latest three censuses

Low fertility and population aging are important factors affecting economic and social development.From 2000 to 2020,the development of low fertility in ethnic minority areas shows three trends of accelerated declining,slow declining and first falling and then rising,which are the results of the balance between the inhibiting effect of controlling birth policy and the rapidly rising birth cost,and the promoting effect of encouraging birth policy on the fertility desire of social members.The development of aging in ethnic minority areas shows two trends of acceleration and deceleration,which are the results of the balance between the promoting effect of controlling birth policy,the outflow of young and middle-aged labor force and the rise of life expectancy,and the inhibiting effect of the encouraging birth policy,the restraint of talent return and talent introduction.Combined with the development degree of low fertility and aging,we can accurately classify and implement policies for different ethnic minority areas.The areas with serious low fertility and population aging problems need economic policies to change the population age structure as soon as possible.The areas with slight low fertility and population aging problems should carry out ideological policies in priority to maintain the benign structure of population age.

Modelling Key Population Attrition in the HIV and AIDS Programme in Kenya Using Random Survival Forests with Synthetic Minority Oversampling Technique-Nominal Continuous

HIV and AIDS has continued to be a major public health concern, and hence one of the epidemics that the world resolved to end by 2030 as highlighted in sustainable development goals (SDGs). A colossal amount of effort has been taken to reduce new HIV infections, but there are still a significant number of new infections reported. HIV prevalence is more skewed towards the key population who include female sex workers (FSW), men who have sex with men (MSM), and people who inject drugs (PWID). The study design was retrospective and focused on key population enrolled in a comprehensive HIV and AIDS programme by the Kenya Red Cross Society from July 2019 to June 2021. Individuals who were either lost to follow up, defaulted (dropped out, transferred out, or relocated) or died were classified as attrition;while those who were active and alive by the end of the study were classified as retention. The study used density analysis to determine the spatial differences of key population attrition in the 19 targeted counties, and used Kilifi county as an example to map attrition cases in smaller administrative areas (sub-county level). The study used synthetic minority oversampling technique-nominal continuous (SMOTE-NC) to balance the datasets since the cases of attrition were much less than retention. The random survival forests model was then fitted to the balanced dataset. The model correctly identified attrition cases using the predicted ensemble mortality and their survival time using the estimated Kaplan-Meier survival function. The predictive performance of the model was strong and way better than random chance with concordance indices greater than 0.75.

Alzheimer’s Disease among American Minority Populations: An Eco­logical Exploratory Study

A significant public health concern with regards to increasing rates of Alzheimer’s is that it disproportionately affects minority groups in the United States.The present ecological exploratory study uses secondary aggregate data from the fifty United States in the year of 2019.The pur­pose of this study was to address the disparities in Alzheimer’s in minori­ty populations in the US and explore associated factors.The“minority”populations considered were African American and Hispanic populations,and the“majority”population was referred to as“white”.The data were extracted from the United States Census Bureau,the CDC National Cen­ter for Health Statistics,and the Behavioral Risk Factor Surveillance Sys­tem(BRFSS)Dataset.The prevalence rates of Alzheimer’s disease are greatest in both older Hispanic(12.2%)and African Americans(13.8%),compared to older whites(10.3%)in the investigated time period.Our results showed that being over 65 years old(p=.009),with a below-av­erage($62,843)median household income(p=.024),history of stroke(p=.029),and being a part of the Hispanic population(p=.036),were significantly associated with Alzheimer’s mortality rates in the United States.By identifying disparities in access to Alzheimer’s healthcare and at-risk communities,more comprehensive intervention strategies can be developed to promote change and advocate for more Alzheimer’s educa­tion and resource allocation for minority populations.

The relationship between acceptance of illness and quality of life among men who have sex with men living with human immunodeficiency virus:A cross-sectional study

Objectives To explore the relationship between acceptance of illness and quality of life(QOL),and factors associated with acceptance of illness among men who have sex with men(MSM)living with human immunodeficiency virus(HIV).Methods Three hundred and one MSM were recruited from an HIV clinic between August and December 2018.The battery of measurements consisted of the Acceptance of Illness Scale,World Health Organization Quality of Life Questionnaire for HIV brief version,HIV Cognitive Appraisal Scale and a self-designed questionnaire used to collect general information.Pearson correlation test was used to examine the relationship between acceptance of illness and QOL.Univariate and multivariate ordinal logistic regression analyses were used to examine whether the general data(demographic and HIV-related clinical data)and HIV cognitive appraisal were associated with acceptance of illness levels.Results Descriptive analysis showed the acceptance level among MSM living with HIV was moderate(Mean=26.29,SD=5.28).Those with a better acceptance of illness had a better QOL(r=0.69,P<0.01).In the multivariate ordinal logistic regression model,education level,threat appraisals(OR:1.09;95%CI[1.06–1.13]),and controllability appraisals(OR:0.82;95%CI[0.71–0.94])were significantly associated with acceptance of illness.Conclusions Results showed acceptance level was moderate and had a positive correlation with QOL.High level of education,low threat appraisals and high controllability appraisals were related to high illness acceptance.This finding highlights the importance of focusing on HIV-infected MSM with low education and on their perceptions of threat and controllability about HIV-related issues when designing interventions to promote illness acceptance in this vulnerable population.

Yunnan’s Kaleidoscope of Folk Dances

THE 26 minority nationalitiesin the frontier province ofYunnan all have a long his-tory and rich cultural heritage.Weshould like to introduce some of themany folk dances they have createdin this beautiful land.The Hani people in Honghe Pre-fecture in southern Yunnan enjoyhunting.Young Hani girls and boysoften go to the mountains to huntand eXpress their love.When a boywins the heart of his beloved。

A study on population genetic structure of Oryza meyeriana (Zoll. et Mor. ex Steud.) Baill. from Yunnan and its in situ conservation significance

In order to determine genetic diversity of Oryza meyeriana (Zoll. et Mor. ex Steud. ) Baill., 12 enzyme systems encoded by 17 loci were electrophoretically analyzed in 164 individuals of seven populations from Simao Prefecture, Yunnan Province, China. In comparison with those seed plants with the same life history and breeding systems, as well as the other species in the genus Oryza, the species shows rather low levels of genetic diversity (A = 1.1, P = 8.0%, Ho = 0.004 and He = 0. 015) within populations and high genetic differentiation among populations. FST was up to 0. 649, suggesting that 64. 9% of total genetic variability exists among populations. Considering high genetic differentiation among populations from a limited geographic region, most of the populations of the species are worth being protected, and therefore, great natural protection regions should theoretically be established in which a great deal of populations should be involved for developing in situ conservation management. Meanwhile, some priory localities for in situ conservation of O. meyeriana in Yunnan Province, were proposed.

云南傣族、彝族、汉族Citrin缺陷SLC25A13基因突变的筛查

目的:调查云南傣族、彝族、汉族希特林蛋白缺陷SLC25A13基因突变的分布特点。方法:应用等位基因特异性扩增方法(AS-PCR),复合扩增10个SLC25A13基因高频突变位点,用毛细管电泳检测扩增产物和GeneMapper software 5软件,分析2189个样本的SLC25A13基因型,用Sanger测序验证。结果:3个民族均检出CT4(IVS16ins3kb)和CT8(c.851-854del4)突变,汉族群体还发现CT2(c.1638_1660dup23)突变。c.851-854del4在傣族人群的突变类型中占有较高比例(75%)。傣、彝、汉族SLC25A13基因的突变携带率分别为1.2%(1/83)、0.44%(1/227)和1.48%(1/67);傣、彝族分别与汉族突变基因携带率比较无差异(P>0.05)。理论上3个民族的NICCD发病率分别为1/27445、1/206116和1/18151,总发病率为1/30667。结论:希特林缺陷病在云南群体中广泛存在。通过SLC25A13基因突变筛查可以为临床遗传咨询和干预提供参考。

Reducing psychiatric illness in the perinatal period:A review and commentary

This brief overview highlights the global crisis of perinatal psychiatric illness(PPI).PPI is a major contributor to many adverse pregnancy,childbirth,and childhood development outcomes.It contributes to billions of dollars in spending worldwide each year and has a significant impact on the individual,their family,and their community.It is also highly preventable.Current recommendations for intervention and management of PPI are limited and vary considerably from country to country.Furthermore,there are several significant challenges associated with implementation of these recommendations.These challenges are magnified in number and consequence among women of color and/or minority populations,who experience persistent and negative health disparities during pregnancy and the postpartum period.This paper aims to provide a broad overview of the current state of recommendations and implementation challenges for PPI and layout a framework for overcoming these challenges.An equityinformed model of care that provides universal intervention for pregnant women may be one solution to address the preventable consequences of PPI on child and maternal health.Uniquely,this model emphasizes the importance of managing and eliminating known barriers to traditional health care models.Culturally and contextually specific challenges must be overcome to fully realize the impact of improved management of PPI.

The Road to Alleviating Poverty

IN southwest China’s Yunnan Province, the local ethnic minority Nu people live 52 km from the downtown region of the Fugong County, Nujiang Lisu Autonomous Prefecture. However, it takes more than three hours to drive there from their village named Laomudeng due to inconvenient road transportation.

公共健康权益可及性对少数民族流动人口定居意愿的影响

以人口高质量发展支撑中国式现代化,提高人口整体素质是关键。而流动人口公共健康权益的可获得性和可及性程度又是增强流动人口身体素质、定居意愿、城市融入的关键因素。通过分析2018年全国流动人口动态监测数据(China Migrants Dynamic Survey,简称CMDS),聚焦少数民族流动人口在流入地公共健康权益的现状,进一步使用二分变量对数比回归模型实证分析少数民族流动家庭公共健康权益可及性对定居意愿发生比的影响,关注家庭主要劳动力(个体)和家庭其他成员(幼童、孕产妇)公共健康权益可及性对少数民族流动人口定居意愿的影响,并探讨不同特征的少数民族流动人口健康权益可及性对家庭定居意愿的异质性影响。研究发现:一是公共健康权益可获得性、可及性程度对少数民族流动人口定居意愿存在显著正向影响,且影响效应存在差异。家庭成员公共健康权益的影响效应大于个体影响效应,但孕产妇公共健康权益可获得性与家庭定居意愿没有显著相关关系。二是不同健康权益项目对单个个体健康权益可及性的影响权重不同。健康档案项目对个体公共健康权益的影响效应最大,疫苗接种项目对幼童公共健康权益的影响效应最大,孕产妇四项公共健康权益项目的作用相差不大。三是不同个体获得公共健康权益数量越多,其定居意愿发生比越高,但影响效应不同。公共健康权益可及性程度对于受教育程度低、夫妻两地分居、省内跨市流动的少数民族流动人口定居意愿的影响更大。

云南野生猕猴桃资源不同居群遗传多样性及遗传演化关系

应用SSR分子标记研究云南10个地理居群野生猕猴桃的遗传多样性及遗传演化关系。结果表明,不同地理居群间的遗传多样性水平差异不大;居群间的遗传一致度在0.9899~0.9991之间,遗传分化系数(Gst)在0.0011~0.1743之间。威信居群与彝良居群间的遗传一致度最高,遗传距离最小,亲缘关系最近;永善居群与云龙居群间的遗传一致度最低,遗传距离最大,亲缘关系最远。10个居群可分为两大类群,师宗、绥江、西畴和永善4个居群聚为类群Ⅰ,麻栗坡、屏边、云龙、镇雄、彝良和威信6个居群聚为类群Ⅱ。类群Ⅰ内的遗传演化关系可分为2个进化层次,师宗居群与绥江居群之间以及西畴居群与永善居群之间的遗传进化关系最近。类群Ⅱ内的遗传演化关系可分为5个进化层次,麻栗坡居群与屏边居群之间的遗传进化关系最近,云龙居群与威信居群之间的亲缘关系较远。研究结果可为加快云南猕猴桃属种质资源的研究利用提供参考。

依嵌而融:东部沿海城市各民族交往交流交融的多维互嵌要素研究

东部沿海城市已成为各民族交往交流交融和全方位相互嵌入的重要场域。人口要素、空间互嵌、经济互嵌和文化互嵌构成了民族交往交流交融的社会基础、场域环境、物质条件和精神依归。以天津市为例,在人口基础要素方面,民族成份齐全多元,少数民族流动人口规模增长较快等,使得各民族社会交往的人口资源增量较为丰富。在空间互嵌环境方面,民族空间交往的场域环境存具区际分布结构、民族聚居结构和社区互嵌结构的差异性。在经济互嵌条件方面,民族就业领域存在集中化现象,各民族围绕职业领域和就业分布进行民族生产交往。在文化互嵌依归方面,通过各民族文化互嵌共生,强化中华文化认同与国家认同教育,增进了民族精神交往。要坚持“并重”思维,理性看待民族就业分布集中化现象,在把握客观规律的基础上建设民族互嵌式社区,顺势而为地推动东部沿海城市各民族广泛交往、全面交流和深度交融。