RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holo...RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.展开更多
【目的】了解对荒漠环境有良好适应性的白皮锦鸡儿种子可培养内生细菌的多样性。【方法】采用种子表面灭菌、种皮与种仁分离、可培养细菌分离纯化、对p H及盐的耐受性、16S r RNA基因序列扩增和系统发育分析进行系统研究。【结果】从白...【目的】了解对荒漠环境有良好适应性的白皮锦鸡儿种子可培养内生细菌的多样性。【方法】采用种子表面灭菌、种皮与种仁分离、可培养细菌分离纯化、对p H及盐的耐受性、16S r RNA基因序列扩增和系统发育分析进行系统研究。【结果】从白皮锦鸡儿成熟种子种皮和种仁分别分离纯化到6株和26株内生细菌,其中分离自种皮的菌株最适p H均在9.0以上,且有3株最高可耐受p H 14.0,有5株可耐受10%的Na Cl;分离自种仁的菌株p H耐受范围明显低于种皮,但有12株对Na Cl的耐受性可达10%。16S r RNA基因序列分析显示,30株与芽孢杆菌属(Bacillus sp.)接近,相似率95%-99%,其中13株与枯草芽孢杆菌(Bacillus subtilis)的相似率为96%-99%,11株与地衣芽孢杆菌(Bacillus licheniformis)相似率为95%-99%;1株与单孢菌属(Sphingomonas sp.)相似性为99%,1株与大肠杆菌属(Escherichia sp.)相似性为98%。【结论】白皮锦鸡儿种仁内生菌数量和种类均高于种皮,体现出其可培养内生菌在空间分布的分异性及种类的多样性,且推测其种皮内生菌对盐碱的高度耐受性应与其环境适应性相关。展开更多
基金supported by a grant from the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET)through the AgriBioindustry Technology Development Programfunded by the Ministry of Agriculture,Food and Rural Affairs(MAFRA)(No.314009-3)
文摘RAPD （randomly amplified polymorphic DNA） markers were employed to characterize polymorphisms among 5 provenances of Acacia leucophloea and to detect genetic relatedness of the species with 6 other acacias （A. holosericea, A. auriculiformis, A. mangium, A. dealbata, A. ferruginea, and A. nilotica） widely grown in India. Of 194 markers scored for the provenances, 29.38% exhibited polymorphism. Also, 326 markers were generated among 7 species of Acacia, accounting for 55.82% of the polymorphisms. The fifteen 10-mer primers employed were capable of producing 1-8 polymorphic bands for the provenances, and 6-17 for all seven species of Acacia. The genetic similarity coefficient based on Jaccard＇ s coefficient revealed that provenances Thirumangalam and Dharmapuri were closely related. The dendrogram based on a sequential agglomerative hierarchical non-overlapping （SAHN） clustering analysis grouped 4 provenances of A. leucophloea （Dharapuram, Thirumangalam, Pudukottai and Dharmapuri） into one cluster and the other provenance, Sendurai, into a separate cluster. The genetic similarity matrix for 7 Acacia species showed that A. nilotica and A. dealbata were distantly related, while A. holosericea and A. ferruginea were very closely related. Cluster analysis grouped the species of Acacias into 3 major groups of which A. dealbata alone formed a separate group. The RAPD markers generated 36 provenance-specific markers and 162 species-specific markers that could have strong applications for species identification and tree breeding programs for A. leucophloea and for other Acacia species included in this study.
基金co-financed by the grants from the Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin of Xinjiang,China(BRZD1102)The Hi-Tech R&D Program of China(2011AA10A202)
