Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography

L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum （ATCC 21799） strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） chromatography. The separation conditions including pH, eluent concentration and Lys+ and Lys2+ adsorption isotherms were studied in batch adsorption. The column capacity, eluent flow rate and eluent concentration have been studied in fixed-bed chromatography. Maximum purification rate of lysine was obtained as 0.066 （g/（g·h）） （per gram resin and per hour） at an eluent flow rate of 10 （mL/min） in fixed-bed chromatography. The results obtained from SMB were 0.11 （g/（g·h）） for L-lysine purification rate and 96% for L-lysine recovery.

配位体交换色谱法测定水基料液中单糖含量

为监测工业化模拟移动床(SMB)上料液及分离液中各单糖含量,建立了检测水基料液中葡萄糖、木糖、半乳糖和阿拉伯糖含量的配位体交换色谱方法。方法采用Sepax Carbomix Pb-NP 5(300×7.8 mm ID,5μm,8%交联度)色谱柱,检测器为示差折光检测器。结果各单糖组分在在0.2~20.0 mg·m L^(-1)浓度范围内呈现良好的线性关系,方法检测限在0.115 mg·m L^(-1)至0.124 mg·m L^(-1)之间。该方法仪器系统简单,结果准确。

传统脱盐方法及新型DSSMB脱盐技术

脱盐是将溶液中"化学盐"脱除的精制提纯过程。简述了传统脱盐技术中离子交换法、膜分离法、电渗析法的技术特点和应用现状。着重介绍模拟移动床DSSMB色谱脱盐新技术、设备及其特点。DSSMB脱盐技术具有无需添加酸碱、清洁生产、脱盐效率显著、自动化程度高、适用范围广、同时实现脱盐脱色及多组分分离等优点,尤其适用于复杂物料的分离,极具应用前景和推广价值。