A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl...A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.展开更多
程序性死亡分子1(programmed death-1,PD-1)/PD-1的配体(programmed death ligand,PD-L)属于抑制性共刺激分子,能对免疫反应的产生起负调节作用。PD-1在慢性感染性疾病中的功能缺陷型CD8+T细胞上高表达,导致功能性CD8+T细胞的功能受损,...程序性死亡分子1(programmed death-1,PD-1)/PD-1的配体(programmed death ligand,PD-L)属于抑制性共刺激分子,能对免疫反应的产生起负调节作用。PD-1在慢性感染性疾病中的功能缺陷型CD8+T细胞上高表达,导致功能性CD8+T细胞的功能受损,包括细胞增殖、细胞因子分泌和杀伤能力。在肿瘤、自身免疫性疾病和感染性疾病中,PD-1/PD-L参与免疫反应的调节,抑制抗瘤及抗病毒免疫反应,阻断此通路后可以恢复特异性CD8+T细胞的部分功能,因此,PD-1/PD-L通路可能与肿瘤细胞免疫逃避和病毒感染疾病慢性化有关。展开更多
文摘A novel chiral stationary phase(CysCSP) for ligand exchange chromatography was prepared by firstly synthesizing N,S-di(2-hydroxyl-3-octoxyl)propyl-L-cysteine as chiral selector via reaction of L-cysteine with glycidyl octyl ether and then coating it on YWG-C-{18} bonded stationary phase. The enantiomeric resolutions of some D,L-α-amino acids were achieved on CysCSP by using cupric acetate aqueous solution as the mobile phase, under the conditions of column temperature 20 ℃ and detection at UV 254 nm. The enantioselectivities α of D,L-α-amino acids separated were found to be in the range from 1.11 to 1.38 with the resolution R-s ranging from 1.1 to 2.8 and the column efficiency being from 5 000 to 9 000 n/m. The elution order of D-isomer before L-isomer was obersaved for all D,L-amino acids used.
文摘程序性死亡分子1(programmed death-1,PD-1)/PD-1的配体(programmed death ligand,PD-L)属于抑制性共刺激分子,能对免疫反应的产生起负调节作用。PD-1在慢性感染性疾病中的功能缺陷型CD8+T细胞上高表达,导致功能性CD8+T细胞的功能受损,包括细胞增殖、细胞因子分泌和杀伤能力。在肿瘤、自身免疫性疾病和感染性疾病中,PD-1/PD-L参与免疫反应的调节,抑制抗瘤及抗病毒免疫反应,阻断此通路后可以恢复特异性CD8+T细胞的部分功能,因此,PD-1/PD-L通路可能与肿瘤细胞免疫逃避和病毒感染疾病慢性化有关。
