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Chemical synthesis of Ub-AMC via ligation of peptide hydrazides 被引量:3
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作者 LIANG Jun FANG GeMin +4 位作者 HUANG XiuLiang MEI ZiQing LI Juan TIAN ChangLin LIU Lei 《Science China Chemistry》 SCIE EI CAS 2013年第9期1301-1306,共6页
The C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC) is an important probe for fluorescencebased analysis of deubiquitinating enzyme (DUB) activity. It is important to develop more efficien... The C-terminal conjugate of ubiquitin with 7-amino-4-methylcoumarin (Ub-AMC) is an important probe for fluorescencebased analysis of deubiquitinating enzyme (DUB) activity. It is important to develop more efficient methods for the preparation of Ub-AMC because the currently available technology is still expensive for scaled-up production. In the present work we report an efficient strategy for total chemical synthesis of Ub-AMC through ligation of peptide hydrazides. Three peptide segments are assembled via N-to-C sequential ligation and the resulting product is converted to Ub-AMC via TCEP-mediated desulfurization. The synthetic Ub-AMC is shown to have expected biological functions throug 展开更多
关键词 h the measurement of its DUB activity.ligation of peptide hydrazides peptide segment condensation deubiquitinating enzymes UBIQUITIN
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