LIGHT、IFN-γ基因转染人肝癌HepG2细胞凋亡及其Caspase-3和survivin表达

目的观察LIGHT、IFN-γ基因转染人肝癌细胞HepG2的凋亡及其Caspase-3和survivin的表达变化。方法用LIGHT、IFN-γ真核细胞表达质粒pcDNA4C-LIGHT-cDNA、pcDNA4C-IFN-γ-cDNA及其转化的E.coliJM-109感受态大肠杆菌,经质粒提取后,转染人肝癌细胞HepG2,同时设对照组(未转染)。分别于转染后12、24、48 h收集HepG2细胞。流式细胞仪检测细胞凋亡和Caspase-3、survivin。结果转染后细胞凋亡及Caspase-3表达量在对照组、LIGHT单转组、联合转染组呈逐渐递增趋势,survivin表达量呈递减趋势。结论LIGHT转染后能通过调节HepG2细胞Caspase-3及survivin的表达来发挥促细胞凋亡作用,INF-γ能增强LIGHT诱导的HepG2细胞凋亡,且显著上调其Caspase-3的表达。

Quantifying urbanization levels on the Tibetan Plateau with high-resolution nighttime light data

The Tibetan Plateau(TP)is undergoing rapid urbanization.To improve urban sustainability and construct eco-logical security barriers,it is essential to quantify the spatial patterns of urbanization level on the TP,but the existing studies on the topic have been limited by the lack of socioeconomic data.This study aims to quantify the urbanization level on the TP in 2018 with Luojia1-01(LJ1-01)high-resolution nighttime light(NTL)data.Specifically,the compounded night light index is used to quantify spatial patterns of urbanization level at mul-tiple scales.The results showed that the TP had a low overall urbanization level with a large internal difference.The urbanization level in the northeast,southeast and south of the TP was relatively high,forming three hotspots centered in Xining City,Lhasa City and Shangri-La City,while the urbanization level in the central and western regions was relatively low.The analysis of influencing factors,based on the random forest model,showed that transportation and topography were the main factors affecting the TP’s spatial patterns of urbanization level.The comparison analysis with socioeconomic statistics and traditional NTL data showed that LJ1-01 NTL data can be used to more effectively quantify the urbanization level since it is more advantageous for reflecting the spatial extent of urban land and describing the spatial structure of socioeconomic activities within urban areas.These advantages are attributed to the high spatial resolution of the data,appropriate imaging time and unaf-fected by saturation phenomena.Thus,the proposed LJ1-01 NTL-based urbanization level measurement method has the potential for wide applications around the world,especially in less-developed regions lacking statistical data.Using this method,we refined the measurement of the TP’s urbanization level in 2018 for multiple scales including the region,basin,prefecture and county levels,which provides basic information for the further urban sustainability research on the TP.

Highly enhanced visible-light photocatalytic hydrogen evolution on g-C_3N_4 decorated with vopc through π-π interaction

Photocatalytic H2 evolution reactions on pristine graphitic carbon nitrides(g-C3N4),as a promising approach for converting solar energy to fuel,are attractive for tackling global energy concerns but still suffer from low efficiencies.In this article,we report a tractable approach to modifying g-C3N4 with vanadyl phthalocyanine(VOPc/CN)for efficient visible-light-driven hydrogen production.A non-covalent VOPc/CN hybrid photocatalyst formed viaπ-πstacking interactions between the two components,as confirmed by analysis of UV-vis absorption spectra.The VOPc/CN hybrid photocatalyst shows excellent visible-light-driven photocatalytic performance and good stability.Under optimal conditions,the corresponding H2 evolution rate is nearly 6 times higher than that of pure g-C3N4.The role of VOPc in promoting hydrogen evolution activity was to extend the visible light absorption range and prevent the recombination of photoexcited electron-hole pairs effectively.It is expected that this facile modification method could be a new inspiration for the rational design and exploration of g-C3N4-based hybrid systems with strong light absorption and high-efficiency carrier separation.

Comparison of Low Stress Mechanical Properties of Light Weight Wool and Wool Blend Fabrics using the KES-F and FAST Instruments

This study compares the test results of the FAST (Fabric Assurance by Simple Testing) with those of the KES - F (Kawabata Evaluation Systems for Fabrics) for a range of nineteen light weight wool and wool blend fabrics in terms of the low - stress mechanical properties of bending, shear, and tensile deformation. It is found that there are very significant correlations between the corresponding parameters for extensibility and shear rigidity obtained from the test results of the two systems. The correlation between the values of bending rigidity obtained from the two systems is only moderate. Furthermore, for the fabrics tested in this study, the values of bending rigidity, shear rigidity, and extensibility measured using the KES - F instruments are higher than those of the corresponding parameters measured using the FAST instruments. The linear regression equation is given for each pair of corresponding parameter.

Permeation Characteristics of Light Hydrocarbons Through Poly（amide-6-β-ethylene oxide） Multilayer Composite Membranes

In this paper, poly(amide-6-β-ethylene oxide) (PEBA1657) copolymer was used to prepare multilayer polyetherimide (PEI)/polydimethylsiloxane (PDMS)/PEBA1657/PDMS composite membranes by dip-coating method. Permeation behaviors of ethylene, ethane, propylene, propane, n-butane, methane and nitrogen through the multilayer composite membranes were investigated over a range of operating temperature and pressure. The permeances of light hydrocarbons through PEI/PDMS/PEBA1657/PDMS composite membranes increase with their increasing condensability, and the olefins are more permeable than their corresponding paraffins. For light hydrocarbons, the gas permeances increase significantly as temperature increasing. When the transmembrane pressure difference increases, the gas permeance increases moderately due to plasticization effect, while their apparent activation energies for permeation decrease.

Tuning the particle size,physical properties,and photocatalytic activity of Ag_(3)PO_(4)materials by changing the Ag^(+)/PO_(4)^(3-)ratio

This study demonstrates the influence of the Ag^(+)/PO_(4)^(3-)ratio in precursor solution on the crystal structural formation,morphology,physical properties,and photocatalytic performance of a Ag_(3)PO_(4)photocatalyst that is fabricated,using a facile precipitation method,from AgNO_(3)and Na2HPO_(4)·12H_(2)O.The material characterizations were carried out using x-ray diffraction(XRD),scanning electron microscopy(SEM),energy-dispersive x-ray spectroscopy(EDX),Brunauer–Emmett–Teller(BET)surface area,Fourier transform infrared(FTIR)absorption,Raman scattering,x-ray photoelectron spectroscopy(XPS),UV-vis absorption,and photoluminescence(PL).The results show that Ag_(3)PO_(4)crystallizes better when the excess PO_(4)^(3-)content increases,and the lattice parameters decrease slightly,while the crystal diameter and the particle size increase.This change is also observed in the Raman scattering and FTIR spectra with the increase in the vibration frequency of the[PO_(4)]group.The compression of the[PO_(4)]unit was also confirmed in the XPS spectra with the shift of P 2p peaks toward higher binding energy.The photocatalytic results showed that the samples synthesized from excess PO_(4)^(3-)solution exhibited higher photocatalytic performance compared to the sample with a Ag^(+)/PO_(4)^(3-)ratio of 3:1.A sample prepared from the precursor solution with a Ag^(+)/PO_(4)^(3-)ratio of 3:1.5 was optimal for RhB decomposition under both visible light and natural sunlight,completely decomposing 10 ppm RhB after 15 minutes of xenon lamp irradiation and after 60 minutes under solar light irradiation.This is attributed to the high crystallinity,small particle size and low electron–hole recombination rate of the sample.

Application of color structured light pattern to measurement of large out-of-plane deformation

Measurement of out-of-plane deformation is significant to understanding of the deflection mechanisms of the plate and tube structures.In this study,a new surface contouring technique with color structured light is applied to measure the out-of-plane deformation of structures with one-shot projection.Through color fringe recognizing,decoding and triangulation processing for the captured images corresponding to each deformation state,the feasibility of the method is testified by the measurement of elastic deflections of a flexible square plate,showing good agreement with those from the calibrated displacement driver.The plastic deformation of two alloy aluminum rectangular tubes is measured to show the technique application to surface topographic evaluation of the buckling structures with large displacements.

Population Spatial Distribution Based on Luojia 1-01 Nighttime Light Image:A Case Study of Beijing

With the continuous development of urbanization in China,the country’s growing population brings great challenges to urban development.By mastering the refined population spatial distribution in administrative units,the quantity and agglomeration of population distribution can be estimated and visualized.It will provide a basis for a more rational urban planning.This paper takes Beijing as the research area and uses a new Luojia1-01 nighttime light image with high resolution,land use type data,Points of Interest(POI)data,and other data to construct the population spatial index system,establishing the index weight based on the principal component analysis.The comprehensive weight value of population distribution in the study area was then used to calculate the street population distribution of Beijing in 2018.Then the population spatial distribution was visualize using GIS technology.After accuracy assessments by comparing the result with the WorldPop data,the accuracy has reached 0.74.The proposed method was validated as a qualified method to generate population spatial maps.By contrast of local areas,Luojia 1-01 data is more suitable for population distribution estimation than the NPP/VIIRS(Net Primary Productivity/Visible infrared Imaging Radiometer)nighttime light data.More geospatial big data and mathematical models can be combined to create more accurate population maps in the future.

LIGHT在低氧性肺动脉高压形成中的作用及机制

目的初步探讨LIGHT在低氧性肺动脉高压(hypoxic pulmonary hypertension,HPH)形成中的作用及其机制。方法将20只8周龄雌性C57BL/6J小鼠[体质量(17.90±0.91)g]和20只8周龄雌性LIGHT-/-C57BL/6J小鼠[体质量(17.55±0.93)g]分为4组(n=10):(1)野生小鼠常氧组(WT-C组)、(2)野生小鼠低氧组(WT-H组)、(3)LIGHT KO小鼠常氧组(LIGHT KO-C组)、(4)LIGHT KO小鼠低氧组(LIGHT KO-H组)。WT-H组和LIGHT KO-H组小鼠置于模拟6 000 m低压舱内连续低氧饲养30 d,WT-C组和LIGHT KO-C组小鼠舱外(海拔308 m)常规饲养。检测右心室收缩压(right ventricular systolic pressure,RVSP)和右心肥厚指数(right ventricular hypertrophy index,RVHI);HE染色观察肺小动脉结构;免疫组化检测LIGHT及其受体HVEM、LTβR表达;荧光定量PCR和Western blot检测肺组织LIGHT、HVEM和LTβR、IL-6的mRNA和蛋白水平。流式细胞术检测肺组织中各类炎症细胞的比例。结果与WT-C组相比,WT-H组肺组织中LIGHT的mRNA和蛋白水平均显著增高(P<0.05),WT-H组RVSP和RVHI明显升高(P<0.05),肺小动脉明显增厚;与LIGHT KO-C组相比,LIGHT KO-H组小鼠的RVSP、RVHI和肺小动脉厚度显著增加(P<0.05),但与WT-H组相比,LIGHT KO-H组的RVSP、RVHI和肺小动脉增厚程度明显降低(P<0.05)。与WT-C组相比,WT-H组LIGHT受体HVEM表达增加,LTβR表达降低,差异具有统计学意义(P<0.05)。LIGHT KO-H组与WT-H组相比,肺组织IL-6 mRNA和蛋白水平显著降低(P<0.05)。流式细胞检测发现,与WT-C组相比,WT-H组小鼠肺组织中单核细胞比例降低[(3.88±0.87)%vs(11.03±1.71)%,P<0.05],间质巨噬细胞比例升高[(15.56±2.69)%vs(8.57±2.17)%,P<0.05];与WT-H组相比,LIGHT KO-H组的肺组织单核细胞增加[(6.55±1.01)%vs(3.88±0.87)%,P<0.05],而间质巨噬细胞的比例降低[(10.87±1.68)%vs.(15.56±2.69)%,P<0.05]。结论慢性低氧诱导肺组织中LIGHT表达增加与HPH发病机制密切相关。LIGHT可能通过HVEM信号途径促进细胞增殖、上调肺组织IL-6表达、促进肺间质巨噬细胞产生,参与HPH的形成。

先天性冠状动脉-肺动脉瘘64层CT诊断1例报告

1临床资料患者,男性,70岁,因反复心悸、头昏、黑蒙5 a,彩超显示心影增大。2 CT心脏冠状动脉成像采用64层Light Speed VCT（GE公司）,增强扫描采用双筒高压注射器（STELLANT,MEDRAD公司）、非离子型造影剂碘帕醇（碘的质量浓度为370 g/L,GE公司）。控制患者心率至65-75次／min。心率较快者可于检查前1h含服25～100mg美托洛尔。患者平卧，连接心电门控，

Ⅱ-Ⅵ族量子点掺杂玻璃的光学性质及研究进展

由于量子限域效应,尺寸可调的Ⅱ-Ⅵ族量子点掺杂玻璃在光学滤波片、非线性光学器件上的应用已经被广泛研究。玻璃中量子点的光学性质主要由量子点的尺寸、表面状态和周围基质环境决定,通过提高Se/Cd比可以有效地对量子点的表面缺陷进行钝化,实现CdSe量子点的本征发光;进一步调整热处理制度可以促进Zn离子扩散进入CdSe量子点表面,形成CdSe/Cd_(1-x)Zn_xSe核壳结构,使得缺陷发光几乎完全猝灭,从而提高量子点的荧光量子效率;在玻璃中原位合成的CdS/ZnS核壳结构量子点的荧光量子效率可达到53%。随着基础研究中玻璃中Ⅱ-Ⅵ族量子点荧光效率的不断提高,发光二极管(LED)等小型发光器件的制造成为可能。为了满足实际需要,建立核壳结构中量子点表面钝化机理模型,进一步优化量子点荧光效率是下一步需要解决的问题。

The effect of slight to minor biodegradation on C_6 to C_7 light hydrocarbons in crude oils: a case study from Dawanqi Oilfield in the Tarim Basin,NW China

Light hydrocarbons （LHs） are one of the main petroleum fractions in crude oils, and carry much infor- mation regarding the genetic origin and alteration of crude oils. But secondary alterations--especially biodegrada- tion--have a significant effect on the composition of LHs in crude oils. Because most of the LHs affected in oils underwent only slight biodegradation （rank 1 on the biodegradation scale）, the variation of LHs can be used to describe more the refined features of biodegradation. Here, 23 crude oils from the Dawanqi Oilfield in the Tarim Basin, NW China, eleven of which have been biodegraded to different extents, were analyzed in order to investigate the effect of slight to minor biodegradation on C6--C7 LHs. The study results showed that biodegradation resulted in the prior depletion of straight-chained alkanes, followed by branched alkanes. In slight and minor biodegraded oils, such biodegradation scale could not sufficiently affect C6- C7 cycloalkanes. For branched C6--C7 alkanes, generally, monomethylalkanes are biodegraded earlier than dimethylalkanes and trimethylalkanes, which indicates that branched alkanes are more resistant to biodegradation, with the increase of substituted methyl groups on parent rings. The degree of alkylation is one of the primary controlling factors on the biodegradation of C6-Cv LHs. There is a particular case： although 2,2,3-trimethylbutane has a rela- tive higher alkylation degree, 2,2-dimethylpentane is more resistant to biodegradation than 2,2,3-trimethylbutane. 2,2- Dimethylpentane is the most resistant to biodegradation in branched C6-C7 alkanes. Furthermore, the 2-methylpen- tane/3-methylpentane and 2-methylhexane/3-methylhexane ratios decreased steadily with increasing biodegradation, which implies that isomers of bilateral methyl groups are more prone to bacterial attack relative to mid-chain iso- mers. The position of the alkyls on the carbon skeleton is also one of the critical factors controlling the rate of biodegradation. With increasing biodegradation, Mango＇s LH parameters K1 values decrease and K2 values increase, the values of n-heptane and isoheptane decrease, and the indices of methylcyclohexane and cyclohexane increase. LH parameters should be applied cautiously for the biodegraded oils. Because biodegraded samples belong to slight or minor biodegraded oils, the values of n-heptane and isoheptane from Dawanqi Oilfield can better reflect and determine the ＂Biodegraded＂ zone. When the heptane value is 0-21 and the isoheptane value is 0-2.6, the crude oil in Dawanqi Oilfield is defined as the ＂Biodegraded＂ zone

Tracking leaf area index and coefficient of light extinction over the harvesting cycle of black wattle

The amount of photosynthetic radiation inter- cepted by a crop is a function of the incident solar radiation on the plants, the leaf area index （LAI）, and the light extinction coefficient （k）. We quantified LAI and k in stands of black wattle （Acacia mearnsii De Wild.） over a 7-year growth cycle at two locations in the state of Rio Grande do Sul, Brazil. Our study was conducted in commercial stands in agroecological regions with high densities of black wattle plantations. LAI was calculated as the ratio between the leaf area of a tree and its planting space, and k was derived from Beer＇s law. LAI depends on the planting site and stand age. Between the two sites, the LAI was similar over time, the amount of variation differed. Values of k depended only on stand age, with the highest average observed for stands up to 5 years old. The trend of k during the plantation cycle was inversely proportional to LAI and was correlated with LAI, leaf area, leaf dry mass, canopy volume, height, branches dry mass, total dry mass, and crown diameter.

Growth and properties of wide spectral white light emitting diodes

Wide spectral white light emitting diodes have been designed and grown on a sapphire substrate by using a metal-organic chemical vapor deposition system. Three quantum wells with blue-light-emitting, green-light-emitting and red-light-emitting structures were grown according to the design. The surface morphology of the film was observed by using atomic force microscopy. The films were characterized by their photoluminescence measurements. X-ray diffraction t9/2/9 scan spectroscopy was carried out on the multi-quantum wells. The secondary fringes of the symmetric ω/2θ X-ray diffraction scan peaks indicate that the thicknesses and the alloy compositions of the individual quantum wells are repeatable throughout the active region. The room temperature photoluminescence spectra of the structures indicate that the white light emission of the multi-quantum wells is obtained. The light spectrum covers 400 700 nm, which is almost the whole visible light spectrum.

OBSERVATION OF AN OUTBURST OF COMET HALE-BOPP IN SEPTEMBER 1996

The light curve and images of comet Hale-Bopp(C/1995 O1) in Sept. 1996 are studied. It is found that an outburst happened in Sept. 10-11, 1996. The magnitude of the inner coma region increased by 2.1 mag. and 1.4 mag. on these two days, respectively. Two globular ejecta were found near the nucleus of the comet in Sept.10 and 11, 1996. By extropolating backward from the ejecta, it is found that the initial time of the outburst was on Sept. 9, 1996 (9.5±0.2UT). The physical reason for this outburst is still uncertain. The projected radial velocities of ejecta are 120m/s and 97m/s.

基于计算机视觉的汽车仪表指针检测方法

最常用的汽车仪表为指针式仪表,工业上汽车仪表指针的检测主要是对指针角度和指针导光性能的检测。汽车仪表检测的准确性是汽车质量控制的关键。目前我国汽车行业普遍采用人工仪表检测方式,劳动强度大,质量控制不稳定。根据指针式汽车仪表图像的特点,采用最小灰度相减法从两张背景相同而仪表指针位置不同的图像中提取到仪表指针,然后运用二值化、细化和Hough变换识别出指针角度。同时,利用提取的指针实现对指针的导光性能检测。实验结果表明,算法具有鲁棒性强、准确性高、实时性好等优点,已成功应用于基于计算机视觉的汽车仪表自动检测系统。

空间遥感器两维扫描指向镜的轻量化设计及应用

论述了空间遥感器中两维扫描指向镜的轻量化设计应注意的主要问题,并给出了扫描指向镜的轻量化设计的应用实例,利用有限元软件,结合设计指标,对其进行了光、机、热综合分析。

指针式压力表自动读数系统设计

为了降低人工读数带来的误差,设计了一个用于指针式压力表自动检定的自动读数系统,该系统主要由CMOS彩色摄像机、DOME光源和计算机三部分构成。DOME光源主要用来降低外界对摄像机拍摄的指针式压力表图像的影响。摄像机获得压力表图像后经USB数据线传输到计算机,计算机首先对图像进行二值化处理,其次,利用Hough变换提取表盘的中心点,再次,利用连通区域标记提取指针和刻度线,最后根据指针和相邻的两条刻度线位置关系并根据刻度线所对应的示值计算出表盘指针对应的刻度值,从而完成自动读数。经实际压力表测试,该系统能够在较短时间内准确读出压力表的示值,极大地解放了劳动力,同时提高了压力表的检定效率。

光针式教学灵敏电流计

介绍了光针式教学灵敏电流计的工作原理和制作方法 .

基于FPGA的激光笔辅助教学系统设计与实现

为了实现激光笔与大屏幕的互动,基于常用的OV9650摄像头模块和315 MHz无线收发模块,采用了一种FPGA架构实现激光笔与大屏幕互动的设计方案。OV9650摄像头模块的采集信号由FPGA进行缓存和处理,由它计算出激光点的坐标,之后计算机接收激光点的坐标及激光笔发出的无线控制信号,通过授课主机端的软件实现激光笔与大屏幕的互动。目前该系统已应用于上海交通大学部分智能教室中,效果明显。