目的探究重组人粒细胞集落刺激因子(recombinant human granulocyte-colony stimulating factor,rhG-CSF)在治疗儿童化疗性口腔黏膜炎中的应用效果。方法选取2020年1月至2022年6月于笔者医院肿瘤外科化疗的60例化疗性口腔黏膜炎患儿,按...目的探究重组人粒细胞集落刺激因子(recombinant human granulocyte-colony stimulating factor,rhG-CSF)在治疗儿童化疗性口腔黏膜炎中的应用效果。方法选取2020年1月至2022年6月于笔者医院肿瘤外科化疗的60例化疗性口腔黏膜炎患儿,按随机数字表法分为两组,每组30例。对照组采取生理盐水口腔护理,实验组采取rhG-CSF口腔护理。比较两组患儿干预效果、口腔黏膜炎分度、生存质量[健康状况调查简表(36-item short—form,SF-36)]、患儿家长护理满意度。结果实验组总有效率较对照组高,差异有统计学意义(P<0.05)。干预5d,实验组患儿口腔黏膜炎分度结果优于对照组,差异有统计学意义(P<0.05)。干预5d,两组患儿SF-36评分较干预前高,实验组较对照组高,差异有统计学意义(P<0.05)。实验组患儿家长满意度较对照组高,差异有统计学意义(P<0.05)。结论rhG-CSF口腔护理可有效提高儿童化疗性口腔黏膜炎的干预效果,减轻口腔黏膜炎分度,改善患儿生存质量,提高患儿家长护理满意度。展开更多
目的:观察益心利水方联合重组人脑利钠肽治疗急性心力衰竭的临床疗效。方法:选取2021年6月至2023年6月在郑州市第九人民医院老年医学科接受治疗的急性心力衰竭患者82例,按照随机数字表法分为对照组和观察组,每组41例。对照组给予重组人...目的:观察益心利水方联合重组人脑利钠肽治疗急性心力衰竭的临床疗效。方法:选取2021年6月至2023年6月在郑州市第九人民医院老年医学科接受治疗的急性心力衰竭患者82例,按照随机数字表法分为对照组和观察组,每组41例。对照组给予重组人脑利钠肽静脉注射治疗,观察组在对照组治疗的基础上给予自拟益心利水方治疗。观察两组患者治疗前后心功能[左心室射血分数(left ventricular ejection fraction,LVEF)、左心室舒张末期内径(left ventricular end diastolic dimension,LVEDD)]、肌酸激酶同工酶(creatine kinase isoenzymes-MB,CK-MB)、超敏肌钙蛋白I(high sensitivity cardiac troponin,hs-cTnI)、N末端脑钠肽前体(N terminal pro B type natriuretic peptide,NT-proBNP)及临床疗效、并发症发生情况。结果:观察组治疗后LVEF高于对照组,LVEDD低于对照组,差异有统计学意义(P<0.05);观察组治疗后CK-MB、hs-cTnI均低于对照组,差异有统计学意义(P<0.05);观察组治疗后NT-proBNP低于对照组,差异有统计学意义(P<0.05);观察组有效率为85.36%,对照组有效率为60.93%,观察组有效率高于对照组,差异有统计学意义(P<0.05)。观察组并发症发生率与对照组比较,差异无统计学意义(P>0.05)。结论:益心利水方联合人脑利钠肽可以有效改善急性心力衰竭患者心功能,降低CK-MB、hs-cTnI,降低心力衰竭标志物NT-proBNP水平。展开更多
An anti-metastatic polypeptide, bifunctional-domain (Cell Ⅰ -Hep Ⅱrecombinant polypeptide of human fibronectin. was expressed in E. coli and purified. The expression level was found to be about 20% 30 % of the tota...An anti-metastatic polypeptide, bifunctional-domain (Cell Ⅰ -Hep Ⅱrecombinant polypeptide of human fibronectin. was expressed in E. coli and purified. The expression level was found to be about 20% 30 % of the total cell proteins. In BL21 (DE3)/T7, an E. coli expressing system, lactose can be used as an inducer to substitute IPTG thereby reducing the cost by several hundredfold and it is suitable for the large-scale preparation of recombinant FN polypeptide. Cell Ⅰ -Hep Ⅱ fragment is an alkaline polypeptide. In BL21 (DE3)/T7 expressing system' better isolation was achieved if DEAE-52, instead of CM-52,was used for ion-exchange chromatography. The purified product was obtained after heparin-agarose affinity chromatography following ion-exchange chromatography.展开更多
Objective To observe the expression of hypoxia inducible factor-1α (HIF-1α) in the retina of rabbits with acute high intraocular pressure and to investigate the mechanism of systemic domestic recombinant human eryth...Objective To observe the expression of hypoxia inducible factor-1α (HIF-1α) in the retina of rabbits with acute high intraocular pressure and to investigate the mechanism of systemic domestic recombinant human erythropoietin (rhEPO) protecting the retina from ischemia-reperfusion injury. Methods First,control group and model group were established in rabbit eyes. The acute high intraocular pressure model was established by saline perfusion into anterior chamber,and then hypodermic injection of domestic rhEPO was made. HIF-1α protein in the retina was observed by immunohistochemical staining method on days 1,3,7 and 14 after retinal ischemia-reperfusion,respectively. Results No cells with HIF-1α positive expression were observed in the retina of the control group. Cells with HIF-1α positive expression in the model group outnumbered those in the control group (P<0.01). The resemblance pattern occurred in EPO group but its degree was slightly greater than that in the model group from day 3 after ischemia-reperfusion (P<0.05). Conclusion Domestic rhEPO can down-regulate the expression of HIF-1α in the retina with acute high intraocular pressure,which may be one of the mechanisms that rhEPO protects the retina from ischemia-reperfusion injury.展开更多
目的:探讨重组人表皮生长因子(rh-EGF)外用溶液治疗放射性口腔黏膜炎(RIOM)的效果。方法:选取2020年1月—2022年3月89例接受放疗并出现RIOM的头颈部肿瘤患者作为研究对象,根据随机数表法分为对照组(n=44)和研究组(n=45)。对照组接受地...目的:探讨重组人表皮生长因子(rh-EGF)外用溶液治疗放射性口腔黏膜炎(RIOM)的效果。方法:选取2020年1月—2022年3月89例接受放疗并出现RIOM的头颈部肿瘤患者作为研究对象,根据随机数表法分为对照组(n=44)和研究组(n=45)。对照组接受地塞米松+庆大霉素+利多卡因稀释液漱口,研究组在对照组治疗基础上加用rh-EGF治疗。采用数字评价量表(numerical rating scale,NRS)和成人癌症生存者生命质量量表(quality of life in adult cancer survivors,QLACS)评估两组口腔疼痛程度与生活质量。比较两组疗效、口腔疼痛及生活质量的差异。结果:研究组近期总有效率为95.56%(43/45),而对照组仅77.27%(34/44),两组比较差异有统计学意义(χ^(2)=7.299,P=0.026)。两组治疗前NRS、QLACS评分比较,差异均无统计学意义(P>0.05);研究组治疗后NRS、QLACS评分均低于对照组,且差异均有统计学意义(P<0.05)。结论:RIOM患者采用重组人表皮生长因子治疗,可以有效缓解疼痛,提高治疗效果,提高生活质量,值得推广应用。展开更多
Background：Mounting evidence has demonstrated that hypoxia-inducible factor-1α （HIF-1α） could attenuate brain injuries after cerebral ischemia and reperfusion （CIR）.However,few reports have addressed the therap...Background：Mounting evidence has demonstrated that hypoxia-inducible factor-1α （HIF-1α） could attenuate brain injuries after cerebral ischemia and reperfusion （CIR）.However,few reports have addressed the therapeutic efficacies of a recombinant adenovirus vector containing HIF-1o （AdHIF-1o） gene after ischemia and reperfusion.The aim of this study was to examine the antiapoptotic and neuroprotective effects ofAdHIF-1o gene for cerebral injuries after ischemia and reperfusion in rats.Methods：From February to December 2016,male Sprague-Dawley rats were randomly divided into normal,sham,CIR,AdHIF-1α,and recombinant adenovirus （Ad） groups.Middle cerebral artery occlusion model was established by Longa＇s method and reperfusion resumed at 2 h postocclusion.AdHIF-1α solution,Ad solution,and phosphate-buffered saline were injected into the right lateral ventricle of rats in AdHIF-lα,Ad,and CIR groups.Brain tissue sections were observed under fluorescent microscope to confirm the definite expression of recombinant adenovirus in Ad and AdHIF-1o groups.The expressions of HIF-lα protein were analyzed by immunohistochemical staining at 6 h,24 h,and 72 h postreperfusion.Brain water content and neurological deficit scores were evaluated at 6 h,24 h,and 72 h postreperfusion.Pathological brain injuries were examined after hematoxylin and eosin stain and nerve cell apoptosis was measured after terminal-deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） stain at 72 h postreperfusion.Comparisons were conducted with one-way analysis of variance by post hoc Scheffe＇s test among different experimental groups.Results：Green fluorescent protein was successfully expressed in brain tissue ofAd andAdHIF-1α groups from 24 h to 21 days postinjection.As detected by immunohistochemical staining,the expressions of HIF-lα protein were obviously enhanced in AdHIF-1o group than those in CIR and Ad groups at 24 h and 72 h postreperfusion,respectively.There were significant reductions of brain water content （78.83% ± 0.34% vs.83.21% ± 0.50% and 83.35% ± 0.32%;84.13% ± 0.24% vs.89.76% ± 0.34% and 89.70% ± 0.18%;respectively;all P 〈 0.05） and neurological deficit scores （2.90 ± 0.74 vs.3.50 ± 0.52 and 3.60 ± 0.53 at 24 h;2.40 ± 0.84 vs.3.60 ± 0.52 and 3.50 ± 0.53 at 72 h;respectively;all P 〈 0.05） in AdHIF-1 α group versus CIR and Ad groups at 24 h and 72 h postreperfusion,respectively.The pathologic changes ofAdHIF-1 α group were milder than those in CIR and Ad groups at 72 h postreperfusion.The percentage of TUNEL-positive cells in cerebral subcortex decreased significantly in AdHIF-1α group versus CIR and Ad groups at 72 h postreperfusion （P 〈 0.05）.Conclusion：AdHIF-1α has an obvious neuroprotective effect on ischemia and reperfusion in rat brains possibly through inhibiting the apoptosis of nerve cells.展开更多
文摘目的:观察益心利水方联合重组人脑利钠肽治疗急性心力衰竭的临床疗效。方法:选取2021年6月至2023年6月在郑州市第九人民医院老年医学科接受治疗的急性心力衰竭患者82例,按照随机数字表法分为对照组和观察组,每组41例。对照组给予重组人脑利钠肽静脉注射治疗,观察组在对照组治疗的基础上给予自拟益心利水方治疗。观察两组患者治疗前后心功能[左心室射血分数(left ventricular ejection fraction,LVEF)、左心室舒张末期内径(left ventricular end diastolic dimension,LVEDD)]、肌酸激酶同工酶(creatine kinase isoenzymes-MB,CK-MB)、超敏肌钙蛋白I(high sensitivity cardiac troponin,hs-cTnI)、N末端脑钠肽前体(N terminal pro B type natriuretic peptide,NT-proBNP)及临床疗效、并发症发生情况。结果:观察组治疗后LVEF高于对照组,LVEDD低于对照组,差异有统计学意义(P<0.05);观察组治疗后CK-MB、hs-cTnI均低于对照组,差异有统计学意义(P<0.05);观察组治疗后NT-proBNP低于对照组,差异有统计学意义(P<0.05);观察组有效率为85.36%,对照组有效率为60.93%,观察组有效率高于对照组,差异有统计学意义(P<0.05)。观察组并发症发生率与对照组比较,差异无统计学意义(P>0.05)。结论:益心利水方联合人脑利钠肽可以有效改善急性心力衰竭患者心功能,降低CK-MB、hs-cTnI,降低心力衰竭标志物NT-proBNP水平。
文摘An anti-metastatic polypeptide, bifunctional-domain (Cell Ⅰ -Hep Ⅱrecombinant polypeptide of human fibronectin. was expressed in E. coli and purified. The expression level was found to be about 20% 30 % of the total cell proteins. In BL21 (DE3)/T7, an E. coli expressing system, lactose can be used as an inducer to substitute IPTG thereby reducing the cost by several hundredfold and it is suitable for the large-scale preparation of recombinant FN polypeptide. Cell Ⅰ -Hep Ⅱ fragment is an alkaline polypeptide. In BL21 (DE3)/T7 expressing system' better isolation was achieved if DEAE-52, instead of CM-52,was used for ion-exchange chromatography. The purified product was obtained after heparin-agarose affinity chromatography following ion-exchange chromatography.
基金supported by the Science and Technology Research Foundation of Shaanxi Province, China (No.2008K14-07)the Medical and Phar macologic Research Foundation of Xi'an City, China (No.SF08004-2)
文摘Objective To observe the expression of hypoxia inducible factor-1α (HIF-1α) in the retina of rabbits with acute high intraocular pressure and to investigate the mechanism of systemic domestic recombinant human erythropoietin (rhEPO) protecting the retina from ischemia-reperfusion injury. Methods First,control group and model group were established in rabbit eyes. The acute high intraocular pressure model was established by saline perfusion into anterior chamber,and then hypodermic injection of domestic rhEPO was made. HIF-1α protein in the retina was observed by immunohistochemical staining method on days 1,3,7 and 14 after retinal ischemia-reperfusion,respectively. Results No cells with HIF-1α positive expression were observed in the retina of the control group. Cells with HIF-1α positive expression in the model group outnumbered those in the control group (P<0.01). The resemblance pattern occurred in EPO group but its degree was slightly greater than that in the model group from day 3 after ischemia-reperfusion (P<0.05). Conclusion Domestic rhEPO can down-regulate the expression of HIF-1α in the retina with acute high intraocular pressure,which may be one of the mechanisms that rhEPO protects the retina from ischemia-reperfusion injury.
文摘目的:探讨重组人表皮生长因子(rh-EGF)外用溶液治疗放射性口腔黏膜炎(RIOM)的效果。方法:选取2020年1月—2022年3月89例接受放疗并出现RIOM的头颈部肿瘤患者作为研究对象,根据随机数表法分为对照组(n=44)和研究组(n=45)。对照组接受地塞米松+庆大霉素+利多卡因稀释液漱口,研究组在对照组治疗基础上加用rh-EGF治疗。采用数字评价量表(numerical rating scale,NRS)和成人癌症生存者生命质量量表(quality of life in adult cancer survivors,QLACS)评估两组口腔疼痛程度与生活质量。比较两组疗效、口腔疼痛及生活质量的差异。结果:研究组近期总有效率为95.56%(43/45),而对照组仅77.27%(34/44),两组比较差异有统计学意义(χ^(2)=7.299,P=0.026)。两组治疗前NRS、QLACS评分比较,差异均无统计学意义(P>0.05);研究组治疗后NRS、QLACS评分均低于对照组,且差异均有统计学意义(P<0.05)。结论:RIOM患者采用重组人表皮生长因子治疗,可以有效缓解疼痛,提高治疗效果,提高生活质量,值得推广应用。
文摘Background：Mounting evidence has demonstrated that hypoxia-inducible factor-1α （HIF-1α） could attenuate brain injuries after cerebral ischemia and reperfusion （CIR）.However,few reports have addressed the therapeutic efficacies of a recombinant adenovirus vector containing HIF-1o （AdHIF-1o） gene after ischemia and reperfusion.The aim of this study was to examine the antiapoptotic and neuroprotective effects ofAdHIF-1o gene for cerebral injuries after ischemia and reperfusion in rats.Methods：From February to December 2016,male Sprague-Dawley rats were randomly divided into normal,sham,CIR,AdHIF-1α,and recombinant adenovirus （Ad） groups.Middle cerebral artery occlusion model was established by Longa＇s method and reperfusion resumed at 2 h postocclusion.AdHIF-1α solution,Ad solution,and phosphate-buffered saline were injected into the right lateral ventricle of rats in AdHIF-lα,Ad,and CIR groups.Brain tissue sections were observed under fluorescent microscope to confirm the definite expression of recombinant adenovirus in Ad and AdHIF-1o groups.The expressions of HIF-lα protein were analyzed by immunohistochemical staining at 6 h,24 h,and 72 h postreperfusion.Brain water content and neurological deficit scores were evaluated at 6 h,24 h,and 72 h postreperfusion.Pathological brain injuries were examined after hematoxylin and eosin stain and nerve cell apoptosis was measured after terminal-deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling （TUNEL） stain at 72 h postreperfusion.Comparisons were conducted with one-way analysis of variance by post hoc Scheffe＇s test among different experimental groups.Results：Green fluorescent protein was successfully expressed in brain tissue ofAd andAdHIF-1α groups from 24 h to 21 days postinjection.As detected by immunohistochemical staining,the expressions of HIF-lα protein were obviously enhanced in AdHIF-1o group than those in CIR and Ad groups at 24 h and 72 h postreperfusion,respectively.There were significant reductions of brain water content （78.83% ± 0.34% vs.83.21% ± 0.50% and 83.35% ± 0.32%;84.13% ± 0.24% vs.89.76% ± 0.34% and 89.70% ± 0.18%;respectively;all P 〈 0.05） and neurological deficit scores （2.90 ± 0.74 vs.3.50 ± 0.52 and 3.60 ± 0.53 at 24 h;2.40 ± 0.84 vs.3.60 ± 0.52 and 3.50 ± 0.53 at 72 h;respectively;all P 〈 0.05） in AdHIF-1 α group versus CIR and Ad groups at 24 h and 72 h postreperfusion,respectively.The pathologic changes ofAdHIF-1 α group were milder than those in CIR and Ad groups at 72 h postreperfusion.The percentage of TUNEL-positive cells in cerebral subcortex decreased significantly in AdHIF-1α group versus CIR and Ad groups at 72 h postreperfusion （P 〈 0.05）.Conclusion：AdHIF-1α has an obvious neuroprotective effect on ischemia and reperfusion in rat brains possibly through inhibiting the apoptosis of nerve cells.
