Photoinhibition and Photooxidation in Leaves of indica and japonica Rice Under Different Temperatures and Light Intensities

Physiological indices related to the efficiency (F-v/F-m) of light energy conversion in PS II and the peroxidation of membrane lipid were measured in leaves of Oryza sativa L. sp. indica rice cv. 'Shanyou 63' and sp. japonica rice cv. '9516'' under different temperatures and fight intensities for 4 days. No changes in F-v/F-m and membrane lipid peroxidation product (MDA) were observed, so neither photoinhibition nor photooxidation happened in both rice cultivars under moderate temperature and medium light intensity. However, F-v/F-m dropped obviously with no change in MDA contents, and photoinhibition appeared in indica rice cv. 'Shanyou 63' under medium temperature and strong light intensity. Furthermore, both photoinhibition and photooxidation were observed in two rice cultivars under chilling temperature and strong light intensity. Experiments with inhibitors under chilling temperature and strong light intensity showed that indica rice had a decrease in DI protein content and SOD activity, and the extent of inhibition of xanthophyll. cycle and nonphotochemical quenching (qN) was larger, and a higher level of MDA was observed. The photoinhibition and photooxidation in indica rice were more distinct as compared with japonica rice. The authors suggested that PS II light energy conversion efficiency (F-v/F-m) and membrane lipid peroxidation were the key indices for the detection of photooxidation.

Growth,nutritional quality,and energy use efficiency in two lettuce cultivars as influenced by white plus red versus red plus blue LEDs

Red plus blue light-emitting diodes(LEDs)are commonly applied in plant factories with artificial lighting due to photosynthetic pigments,which absorb strongly in red and blue light regions of the spectrum.However,plants grown under natural environment are used to utilizing broad-wide spectrum by long-term evolution.In order to examine the effects of addition light added in red plus blue LEDs or white LEDs,green and purple leaf lettuces(Lactuca sativa L.cv.Lvdie and Ziya)were hydroponically cultivated for 20 days under white LEDs,white plus red LEDs,red plus blue LEDs,and red plus blue LEDs supplemented with ultraviolet,green or far-red light,respectively.The results indicated that the addition of far-red light in red plus blue LEDs increased leaf fresh and dry weights of green leaf lettuce by 28%and 34%,respectively.Addition of ultraviolet light did not induce any differences in growth and energy use efficiency in both lettuce cultivars,while supplementing green light with red plus blue LEDs reduced the vitamin C content of green leaf lettuce by 44%and anthocyanin content of purple leaf lettuce by 30%compared with red plus blue LEDs,respectively.Spectral absorbencies of purple leaf lettuce grown under red plus blue LEDs supplemented with green light were lower in green light region compared with those grown under red plus blue LEDs,which was associated with anthocyanin contents.White plus red LEDs significantly increased leaf fresh and dry weights of purple leaf lettuce by 25%,and no significant differences were observed in vitamin C and nitrate contents compared with white LEDs.Fresh weight,light and electrical energy use efficiencies of hydroponic green and purple leaf lettuces grown under white plus red LEDs were higher or no significant differences compared with those grown under red plus blue LEDs.In conclusion,white plus red LEDs were suggested to substitute for red plus blue LEDs in hydroponic lettuce(cv.Lvdie and Ziya)production in plant factories with artificial lighting.

Effects of LED light quality on the growth of pepper seedling in plant factory

The extensive environment,especially low temperature and weak lighting in winter and spring,which limits the growth of pepper(Capicum annuum L.)seedlings,the use of plant factory with artificial lighting technology can effectively control the lighting environment to produce high-quality seedlings.In this study,white LED lamps with R:B ratio of 0.7(L0.7)and 1.5(L1.5)and red-blue LED lamps with R:B ratio of 3.5(L3.5)were used to cultivate seedlings of“CAU-24”pepper in the light intensity of 250μmol/m^(2)·s and photoperiod of 12 h/d,white fluorescent lamps with R:B ratio of 1.7(F1.7)was used as control.The results showed that plant height,stem diameter,hypocotyl length,biomass accumulation,light energy use efficiency(LUE)and electric energy use efficiency(EUE)of pepper seedling under L1.5 were the highest.After 36 days of sowing,the dry weight of shoot reached 302.8±45.2 mg/plant.Leaf area reached maximum value of 153.5±22.0 cm^(2) under L0.7.The contents of chlorophyll a,chlorophyll b and total chlorophyll of pepper seedling leaves under all kinds of LED light were greater than F1.7,but there was no significant difference in net photosynthetic rate.The total dry weight with lamp electric power consumption of L1.5 were 3.0 g/(kW·h)which was 1.5,2,and 3 times greater than that of L3.5,L0.7,and F1.7,respectively.Therefore,compared with fluorescent lamp and other LED lamps,the white LED light quality with R:B ratio of 1.5 is suitable for pepper seedling production in plant factory because of the high LED lighting efficiency,greater LUE and EUE.