Selective hydrolysis of lignocelluloses from corn stalk in an ionic liquid

Although lots of basic studies , such as the hydrolysis and dissolution of lignocelluloses has made great progress in recent years , the hydrolysates containing complex mixture of pentose and hexose are very hard to be separated , and these process sometimes cause serious environmental problems in practical application of cellulose polymer degradation science.Herein , an efficient two-stage method for selective hydrolysis of lignocelluloses biomass is being developed in this paper by controlling of pH in an ionic liquid.The lignin-hemicelluloses matrix in corn stalk was hydrolyzed into xylose in 23.1% yield in the first stage ; and cellulose-rich residues from the first stage was by farther hydrolyzed to provide a glucose in 26.9%yield.Structure of the products were identified by 13 C NMR.It should be mentioned that , the ionic liquid which can be regenerated and reused throughout the process. The present work significantly opens an a new path to utilize each component of lignocellulose as raw materials producing biofuels , renewable energy and fine chemicals.

In-situ polymerization of lignocelluloses of autohydrolysis process with acrylamide

In the present study,the hydrolysates generated via autohydrolysis of spruce wood chips were di-rectly used as feedstock for producing coagulants.The in-situ polymerization of acrylamide(AM)and lignocellulose(LC)of hydrolysates was successfully conducted.The reaction was optimized to generate lignocellulose-acrylamide(LC-AM)with the highest molecular weight(41,060 g/mol)and charge density(-0.25 meq/g)under the optimum conditions,which were 3 h,60◦C,4%(w)initiator based on the dried mass of hydrolysate,and an AM/LC molar ratio of 5.63.A nuclear magnetic resonance(NMR)spectroscopy confirmed the grafting of acrylamide on LC.Other prop-erties of LC-AM were characterized by the elemental analyzer,zeta potential analyzer,gel per-meation chromatography(GPC),and particle charge detector(PCD).The LC-AM was applied as a coagulant for removing ethyl violet dye from a simulated dye solution.The results indicated that 47.2%dye was removed from the solution at a low dosage of 0.2 g/g.The dual flocculation of LC-AM with other polymers for dye removal is suggested to further improve its effectiveness.

A sustainable process to 100%bio-based nylons integrated chemical and biological conversion of lignocellulose

Considerable progress has been made in recent years to the development of sustainable polymers from bio-based feedstocks.In this study,100%bio-based nylons were prepared via an integrated chemical and biological process from lignocellulose.These novel nylons were obtained by the melt polymerization of 3-propyladipic acid derived from lignin and 1,5-pentenediamine/1,4-butanediamine derived from carbohydrate sugar.Central to the concept is a three-step noble metal free catalytic chemical funnelling sequence(Raney Ni mediated reductive catalytic fractionation-reductive funnelling-oxidative funnelling),which allowed for obtaining a single component 3-propyladipic acid from lignin with high efficiency.The structural and thermodynamic properties of the obtained nylons have been systematically investigated,and thus obtained transparent bio-based nylons exhibited higher Mw(>32,000)and excellent thermal stability(Td5%>265℃).Considering their moderate Tg and good melt strength,these transparent bio-based nylons could serve as promising functional additives or temperature-responsive materials.

Acetic acid-and furfural-based adaptive evolution of Saccharomyces cerevisiae strains for improving stress tolerance and lignocellulosic ethanol production

Acetic acid and furfural are known as prevalent inhibitors deriving from pretreatment during lignocellulosic ethanol production.They negatively impact cell growth,glucose uptake and ethanol biosynthesis of Saccharomyces cerevisiae strains.Development of industrial S.cerevisiae strains with high tolerance towards these inhibitors is thus critical for efficient lignocellulosic ethanol production.In this study,the acetic acid or furfural tolerance of different S.cerevisiae strains could be significantly enhanced after adaptive evolution via serial cultivation for 40 generations under stress conditions.The acetic acid-based adaptive strain SPSC01-TA9 produced 30.5 g·L^(-1)ethanol with a yield of 0.46 g·g^(-1)in the presence of 9 g·L^(-1)acetic acid,while the acetic acid/furfural-based adaptive strain SPSC01-TAF94 produced more ethanol of 36.2 g·L^(-1)with increased yield up to 0.49 g·g^(-1)in the presence of both 9 g·L^(-1)acetic acid and 4 g·L^(-1)furfural.Significant improvements were also observed during non-detoxified corn stover hydrolysate culture by SPSC01-TAF94,which achieved ethanol production and yield of 29.1 g·L^(-1)and 0.49 g·g^(-1),respectively,the growth and fermentation efficiency of acetic acid/furfural-based adaptive strain in hydrolysate was 95%higher than those of wildtype strains,indicating the acetic acid-and furfural-based adaptive evolution strategy could be an effective approach for improving lignocellulosic ethanol production.The adapted strains developed in this study with enhanced tolerance against acetic acid and furfural could be potentially contribute to economically feasible and sustainable lignocellulosic biorefinery.

Bacterial communities and enzyme activities during litter decomposition of Elymus nutans leaf on the Qinghai-Tibet Plateau

The dominant plant litter plays a crucial role in carbon(C)and nutrients cycling as well as ecosystem functions maintenance on the Qinghai-Tibet Plateau(QTP).The impact of litter decomposition of dominant plants on edaphic parameters and grassland productivity has been extensively studied,while its decomposition processes and relevant mechanisms in this area remain poorly understood.We conducted a three-year litter decomposition experiment in the Gansu Gannan Grassland Ecosystem National Observation and Research Station,an alpine meadow ecosystem on the QTP,to investigate changes in litter enzyme activities and bacterial and fungal communities,and clarify how these critical factors regulated the decomposition of dominant plant Elymus nutans(E.nutans)litter.The results showed that cellulose and hemicellulose,which accounted for 95%of the initial lignocellulose content,were the main components in E.nutans litter decomposition.The litter enzyme activities ofβ-1,4-glucosidase(BG),β-1,4-xylosidase(BX),andβ-D-cellobiosidase(CBH)decreased with decomposition while acid phosphatase,leucine aminopeptidase,and phenol oxidase increased with decomposition.We found that both litter bacterial and fungal communities changed significantly with decomposition.Furthermore,bacterial communities shifted from copiotrophic-dominated to oligotrophic-dominated in the late stage of litter decomposition.Partial least squares path model revealed that the decomposition of E.nutans litter was mainly driven by bacterial communities and their secreted enzymes.Bacteroidota and Proteobacteria were important producers of enzymes BG,BX,and CBH,and their relative abundances were tightly positively related to the content of cellulose and hemicellulose,indicating that Bacteroidota and Proteobacteria are the main bacterial taxa of the decomposition of E.nutans litter.In conclusion,this study demonstrates that bacterial communities are the main driving forces behind the decomposition of E.nutans litter,highlighting the vital roles of bacterial communities in affecting the ecosystem functions of the QTP by regulating dominant plant litter decomposition.

Electrochemical reduction of carbon dioxide to produce formic acid coupled with oxidative conversion of biomass

Electrochemical reduction of CO_(2)(CO_(2)RR)has become a research hot spot in recent years in the context of carbon neutrality.HCOOH is one of the most promising products obtained by electrochemical reduction of CO_(2) due to its high energy value as estimated by market price per energy unit and wide application in chemical industry.Biomass is the most abundant renewable resource in the natural world.Coupling biomass oxidative conversion with CO_(2)RR driven by renewable electricity would well achieve carbon negativity.In this work,we comprehensively reviewed the current research progress on CO_(2)RR to produce HCOOH and coupled system for conversion of biomass and its derivatives to produce value-added products.Sn-and Bi-based electrocatalysts are discussed for CO_(2)RR with regards to the structure of the catalyst and reaction mechanisms.Electro-oxidation reactions of biomass derived sugars,alcohols,furan aldehydes and even polymeric components of lignocellulose were reviewed as alternatives to replace oxygen evolution reaction(OER)in the conventional electrolysis process.It was recommended that to further improve the efficiency of the coupled system,future work should be focused on the development of more efficient and stable catalysts,careful design of the electrolytic cells for improving the mass transfer and development of environment-friendly processes for recovering the formed formate and biomass oxidation products.

Activated Carbon from Nipa Palm Fronds(Nypa fruticans)with H_(3)PO_(4) and KOH Activators as Fe Adsorbers

Nipa palm is one of the non-wood plants rich in lignocellulosic content.In this study,palm fronds were converted into activated carbon,and their physical,chemical,and morphological properties were characterized.The resulting activated carbon was then applied as an adsorbent of Fe metal in peat water.The carbonization process was carried out for 60 min,followed by sintering at 400℃ for 5 h with a particle size of 200 mesh.KOH and H_(3)PO_(4) were used in the chemical activation process for 24 h.KOH-activated carbon contained 6.13%of moisture,4.55%of ash,17.02%of volatile matter,and 78.84%of fixed carbon,while its Fe reduction efficiency was 28.09%.The H_(3)PO_(4)-activated carbon contained 4.67%of moisture,2.84%of ash,16.41%of volatile matter,and 80.57%of bonded carbon,and the Fe reduction efficiency was 52.25%.KOH-activated carbon and H_(3)PO_(4)-activated carbon contained fixed carbon of 78.84%and 80.57%,respectively,while their average rates of efficiency of Fe reduction were 22.82%and 39.23%,respectively.Overall,the characteristics of activated nipa carbon met the Indonesian standards(SNI No.06-3730-1995).However,H_(3)PO_(4)-activated carbon was found to be better at adsorbing Fe metal from peat water.

Camalote Grass (Paspalum fasciculatum Willd) as a Sustainable Raw Material for the Production of Lignocellulosic Ethanol

The current trend of replacing a percentage of gasoline with ethanol has promoted the development of new processes for its production from lignocellulosic biomass. This work reports the production of ethanol from the Camalote grass (Paspalum fasciculatum Willd). The lignocellulosic biomass was subjected to acid hydrolysis at 125C and 15 psi with H2SO4 concentrations at 5%, 10%, and 20%, obtaining an average of reducing sugars (pentoses and hexoses) from the hydrolyzed juice with 12.3%, 10%, and 17% Brix, respectively. The sugars were fermented using yeast of the Saccharomyces cerevisiae at 30C for 48 hours. Finally, the ethanol was distilled at 78C, and the average yields were obtained through analysis of variance with a 95% confidence level. The values indicate that there is a significant difference (p > 0.05), the Tukey study shows that all the % v/v averages are different from each other. For H2SO4 concentration at 5% (10.33 ± 2), H2SO4 at 10% (9.33 ± 1.8), and H2SO4 at 20% (6.33 ± 2). The acidity analysis for the ethanol obtained from each treatment gave a value of 1.8 mg/L of acetic acid in all cases.

Optimization of Cellulose Nanocrystal Isolation from Ayous Sawdust Using Response Surface Methodology

This study focuses on the extraction of cellulose nanocrystals (CNC), from microcrystalline cellulose (MCC), derived from Ayous sawdust. The process involves multiple steps and a large amount of chemical products. The objective of this research was to determine the effects of factors that impact the isolation process and to identify the optimal conditions for CNC isolation by using the response surface methodology. The factors that varied during the process were the quantity of MCC, the concentration of sulfuric acid, the hydrolysis time and temperature, and the ultrasonic treatment time. The response measured was the yield. The study found that with 5.80 g of microcrystalline cellulose, a sulfuric acid concentration of 63.50% (w/w), a hydrolysis time of 53 minutes, a hydrolysis temperature of 69˚C, and a sonication time of 19 minutes are the ideal conditions for isolation. The experimental yield achieved was (37.84 ± 0.99) %. The main factors influencing the process were the sulfuric acid concentration, hydrolysis time and temperature, with a significant influence (p < 0.05). Infrared characterization results showed that nanocrystals were indeed isolated. With a crystallinity of 35.23 and 79.74, respectively, for Ayous wood fiber and nanocrystalline cellulose were observed by X-ray diffraction, with the formation of type II cellulose, thermodynamically more stable than native cellulose type I.

In situ fabrication of Na3V2（PO4）3 quantum dots in hard carbon nanosheets by using lignocelluloses for sodium ion batteries

The rational assembly of quantum dots on two-dimensional(2 D) carbonaceous materials is very promising to produce materials, but remains a challenge. Here, we develop an assembly strategy of growing Na3 V2(PO4)3 quantum dots with superlattice structure(NVP-QDs-SL) for obtaining precise control of the size, distribution and crystallinity. The multifunctional lignocelluloses(LCs) used as a hard carbon source induce heterogeneous nucleation and confined growth of NVP-QDs-SL, leading to the uniform distribution of NVP-QDs-SL in H/S-doped hard carbon ultra-thin nanosheets(HCS). Detailed electrochemical analysis results from sodium-ion batteries of NVP-QDs-SL show that NVP-QDs-SL could trap the electrons inside HCS, significantly enhancing Na ion storage and transfer kinetics. Compared to the common Na3 V2(PO4)3 nanoparticle cathode, the NVP-QDs-SL/HCS cathode exhibits a high reversible capacity of 149.2 m A h g^-1 at a 0.1 C rate, which is far beyond the theoretical capacity of Na3 V2(PO4)3(117.6 m A h g^-1).At the ultrahigh current rate of 100 C, this cathode still remains a high discharge capacity of 40 m A h g-1.Even after cycling at 20 C over 3000 cycles, an ultrahigh coulombic efficiency close to 100% is still obtained,highlighting its excellent long cycling life, remarkable rate performance and energy density.

Breaking the temperature limit of hydrothermal carbonization of lignocellulosic biomass by decoupling temperature and pressure

Hydrothermal carbonization(HTC) of lignocellulosic biomass is a promising technology for the production of carbon materials with negative carbon emissions. However, the high reaction temperature and energy consumption have limited the development of HTC technology. In conventional batch reactors, the temperature and pressure are typically coupled at saturated states. In this study, a decoupled temperature and pressure hydrothermal(DTPH) reaction system was developed to decrease the temperature of the HTC reaction of lignocellulosic biomass(rice straw and poplar leaves). The properties of hydrochars were analyzed by scanning electron microscopy(SEM), Fourier transform infrared(FTIR) spectroscopy, X-ray photoelectron spectroscopy(XPS), Raman spectroscopy, X-ray diffraction(XRD), thermogravimetric analyzer(TGA), etc. to propose the reaction mechanism. The results showed that the HTC reaction of lignocellulosic biomass could be realized at a low temperature of 200℃ in the DTPH process, breaking the temperature limit(230℃) in the conventional process. The DTPH method could break the barrier of the crystalline structure of cellulose in the lignocellulosic biomass with high cellulose content, realizing the carbonization of cellulose and hemicellulose with the dehydration, unsaturated bond formation, and aromatization. The produced hydrochar had an appearance of carbon microspheres, with high calorific values, abundant oxygen-containing functional groups, a certain degree of graphitization, and good thermal stability. Cellulose acts not only as a barrier to protect itself and hemicellulose from decomposition, but also as a key precursor for the formation of carbon microspheres. This study shows a promising method for synthesizing carbon materials from lignocellulosic biomass with a carbon-negative effect.

Research perspectives for catalytic valorization of biomass

Efficient utilization of biomass for the supply of energy and synthetic materials would mitigate the heavy reliance on fossil resources and the growing CO_(2) emission, thus contributing to establishing sustainable and carbon–neutral societies. Much effort has been devoted to catalytic transformations of lignocellulosic biomass, the most abundant and nonedible form of biomass.

Co@CoO:An efficient catalyst for the depolymerization and upgrading of lignocellulose to alkylcyclohexanols with cellulose intact

The depolymerization and upgrading of lignin from raw biomass,while keeping cellulose intact is important in biorefinery and various metal-based catalysts have been used in reductive catalytic fractionation,a key method in"lignin-first"strategy,Recently,we found that a core-shell structured Co@CoO catalyst with CoO shell as the real active site had excellent performance in the hydrogenolysis of 5-hydromethylfurfural to 2,5-dimethylfuran due to its unique ability to dissociate H_(2)and yield active H^(δ-)species(Xiang et al.,2022).In this work,we report a one-pot depolymerization and upgrading of lignocellulose to alkylcyclohexanols,a flavour precursor,with intact cellulose over this unique core-shell structured catalyst,Co@CoO.Lignin model compounds(β-O-4,4-O-5,α-O-4)were first used to clarify the activity of Co@CoO catalyst.Then,the one-pot conversion of various organosolv lignin(birch,pine and poplar)to alkylcyclohexanols was realized with the mass yield of alkylcyclohexanols up to25.8 wt%from birch lignin under the reaction condition of 210℃,1 MPa H_(2),16 h.Finally,the corresponding woody sawdusts were used as feedstocks and found that the Co@CoO catalyst indeed preferentially depolymerized and upgraded the lignin part and obtained the same alkylcyclohexanols products with the retention of cellulose-rich pulp.The collected alkylcyclohexanols were further esterified to obtain valueadded esters,which can be used as flavors.This work will inspire the design of new efficient metal oxide catalysts in lignin fractionation and depolymerization to high-value-added chemicals with intact cellulose.

The case-dependent lignin role in lignocellulose nanofibers preparation and functional application-A review

Lignocellulose nanofibers(LCNFs) as a new material is attracting extensive attention. The pretreatment and mechanical fibrillation are the two main stages involved in the preparation of LCNFs, and lignin plays the important role of these two stages. This review discussed the interaction between lignin and chemicals in the pretreatment stage, and discovered the general law of the effect of lignin in the mechanical fibrillation stage.Lignin exhibits both promotion and inhibition effects on mechanical fibrillation, and the mutual competition between the two effects ultimately affects the energy consumption, morphology and yield of LCNFs. Furthermore, the recent research progress related to the contributions of lignin on the functional application of LCNFs was summarized, aiming to provide profound guidance for the preparation and application of LCNFs.

Production,purific tion,characterization and application of two novel endoglucanases from buffalo rumen metagenome

Background Lignocellulose biomass is the most abundant and renewable material in nature.The objectives of this study were to characterize two endoglucanases Trep Cel3 and Trep Cel4,and determine the effect of the combination of them(1.2 mg Trep Cel3,0.8 mg Trep Cel4)on in vitro rumen fermentation characteristics.In this study,three nature lignocellulosic substrates(rice straw,RS;wheat straw,WS;leymus chinensis,LC)were evaluated for their in vitro digestibility,gas,NH3-N and volatile fatty acid(VFA)production,and microbial protein(MCP)synthesis by adding enzymatic combination.Methods Two endoglucanases’genes were successfully expressed in Escherichia coli(E.coli)BL21(DE3),and enzymatic characteristics were further characterized.The combination of Trep Cel3 and Trep Cel4 was incubated with lignocellulosic substrates to evaluate its hydrolysis ability.Results The maximum enzymatic activity of Trep Cel3 was determined at p H 5.0 and 40℃,while Trep Cel4 was at p H 6.0 and 50℃.They were stable over the temperature range of 30 to 60℃,and active within the p H range of 4.0 to 9.0.The Trep Cel3 and Trep Cel4 had the highest activity in lichenan 436.9±8.30 and 377.6±6.80 U/mg,respectively.The combination of Trep Cel3 and Trep Cel4 exhibited the highest efficiency at the ratio of 60:40.Compared to maximum hydrolysis of Trep Cel3 or Trep Cel4 separately,this combination was shown to have a superior ability to maximize the saccharification yield from lignocellulosic substrates up to 188.4%for RS,236.7%for wheat straw WS,222.4%for LC and 131.1%for sugar beet pulp(SBP).Supplemental this combination enhanced the dry matter digestion(DMD),gas,NH3-N and VFA production,and MCP synthesis during in vitro rumen fermentation.Conclusions The Trep Cel3 and Trep Cel4 exhibited the synergistic relationship(60:40)and significantly increased the saccharification yield of lignocellulosic substrates.The combination of them stimulated in vitro rumen fermentation of lignocellulosic substrates.This combination has the potential to be a feed additive to improve agricultural residues utilization in ruminants.If possible,in the future,experiments in vivo should be carried out to fully evaluate its effect.

Co-digestion of Waste Coffee and Cocoa Hulls: Modeling of Biogas Production by the Particle Swarm Method

Energy is a crucial material for the development of our economy.Access to sufficient energy remains a major concern for developing countries,particularly those in sub-Saharan Africa.The major challenge lies in access to clean,environmentally friendly,quality and low-cost energy in different households in our municipalities.To cope with this vast energy gap,many households are dependent on fossil fuels.In Cameroon,the consumption of wood for the supply of energy is increasing by 4%per year.Overall,approximately 80%of households in Cameroon depend on woody biomass as the sole main source of energy supply in Cameroon and demand is growing over time.In view of the climatic variations that our countries,particularly Cameroon,undergo through deforestation,the use of wood as a source of energy is expensive and harmful to the environment,hence the urgency of replacing wood with renewable energy.Biogas is one of the most versatile sources of renewable energy.On an industrial scale,it is important to automate the process control.The main objective of the present work is to model the anaerobic digestion of coffee and cocoa hulls using the particle swarm optimisation method.Pretreatment using the organosolv process was done.This resulted in 48%lignin removal and 22%cellulose increase.For the pretreated biomass,the maximum production rate was 21 NmLCH4 per day with a biomethane yield of 90 NmLCH4/gVS.This represents an enhancement of 117%in biomethane yield.A positive flammability test was recorded after the 10th day of retention time.Moreover,the data collected during anaerobic digestion allowed implementation of a two-phase mathematical model.The thirteen parameters of the model were estimated with particle swarm optimisation method in Matlab.The model was able to simulate the biomethane production kinetics and variation of volatile fatty acid concentration.

Optimization and Thermodynamic Studies of Lead (II) and Cadmium (II) Ions Removal from Water Using Musa acuminate Pseudo-Stem Biochar

We recently found out that water from the Ugandan stretch of the Kagera transboundary river (East Africa) is contaminated with lead (Pb2+) and cadmium (Cd2+) ions at levels that are above permissible limits in drinking water. Because lignocellulosic biomass-based adsorbents have been explored for the remediation of metal ions from water, this study investigated the potential of Musa acuminata pseudo-stem (MAPS) biochar for the remediation of Pb2+ and Cd2+ ions from water. Batch adsorption experiments were performed to optimize the adsorption conditions while the isotherms were analyzed using Freundlich and Langmuir models. Results showed that the maximum adsorption capacity at equilibrium was 769.23 mg/g and 588.23 mg/g for Pb2+ and Cd2+ ions, respectively. Langmuir isotherm model provided the best fit for the data, and it was favorable since all r2 values (Cd2+ = 0.9726 and Pb2+ = 0.9592) were close to unity. Gibb’s free energy change was found to be negative for both metals, implying the feasibility of the adsorption process. Correspondingly, the enthalpy change was positive for both metal ions which revealed that the adsorption process was endothermic and it occurred randomly at the solid-liquid interface. These results suggested that biochar from MAPs could be utilized for the removal of Pb2+ and Cd2+ from polluted water in the Kagera transboundary river to make it suitable for domestic use. Further studies should consider chemical modification of the biochar as well as characterization to examine the chemical nature of the biochar.

Carbon Fiber from Biomass Sources:A Comprehensive Review

Global energy demand is rising,fossil fuel prices are rising,fossil fuel reserves are running out,and fossil fuel use contributes to the greenhouse effect.As a clean alternative source of energy to fossil fuels,biomass is becoming more and more essential.Carbon fiber(CF),often known as graphite fiber,is a thin,strong,and adaptable material utilized in both structural(capacity)and non-structural applications(e.g.,thermal insulation).Precursors are the raw materials used to create carbon fiber,which is mostly derived from fossil fuels.Because of the high cost of precursors and manufacture,carbon fiber has only found employment in a few numbers of high-performance structural materials(e.g.,aerospace).To reduce the price of CF and reliance on fossil fuels,numerous alternative precursors have been studied throughout the years,including biomass-derived precursors such as rayon,lignin,glycerol,and lignocellulosic polysaccharides.This study’s goal is to present a detailed study of biomass-derived CF precursors and their market potential.The authors look into the viability of producing CF from these precursors,as well as the state of technology,potential applications,and cost of production(when data are available).We go over their benefits and drawbacks.We also talk about the physical characteristics of CF made from biomass and contrast them with CF made from polyacrylonitrile(PAN).Additionally,we go into bio-based CF manufacturing and end-product concerns,logistics for biomass feedstock and plant sites,feedstock competition,and risk-reduction techniques.This paper offers a comprehensive overview of the CF potential from all biomass sources and can be used as a resource by both novice and seasoned professionals who are interested in producing CF from non-traditional sources.

Comparison of hydroxyl radical-scavenging abilities between white rot fungus Lentinula edodes and five mildews on lignocellulose substance

The hydroxyl radical（-OH）-scavenging ability of culture filtrates from submerged culture of Lentinula edodes AX3 and five mildews on lignocellulose substance was analysed. Only L. edodes AX3 showed significant -OH-scavenging ability which reached 52.2% at about the 48th hour. All mildews could hardly scavenge -OH under the experimental conditions. -OH-scavenging ability is considered related to the mode and ability of lignocellulose degradation of a strain. The degradation or bioconversion products might be the substance base for.this effect.

Production of Cellulase and Xylanase by Aspergillus terreus KJ829487 Using Cassava Peels as Subtrates

Cassava (Manihot esculenta, Crantz) is one of the most important food plants in West Africa. Its peels are made up of cellulose, hemicellulose and lignin. This lignocellulolytic biomass can be converted using microbial enzymes to fermentable sugars which have wide range of biotechnological relevance in many fermentation processes. The aim of this study is to screen filamentous fungi from decaying cassava peels that are good producers of xylanases and cellulases. Decaying parts of cassava peels were obtained and brought to the laboratory for further work. Fungi were isolated, identified and screened for cellulase and xylanase production. Isolate with highest frequency of occurrence and enzyme production was identified using phenotypic and molecular method. Optimisation of growth conditions for enzymes production was monitored using the DNSA method, also saccharification of cassava peel were carried out using the enzymes obtained from the isolate. Aspergillus terreus KJ829487 was the predominant fungus. It produces cellulases and xylanases optimally at 40°C, pH 6 and 8, utilising carboxymethylcellulose (CMC) or xylose and yeast extracts as its carbon and nitrogen sources respectively. Saccharification of the peels yielded 584 mg/L glucose, 78 mg/L xylose and 66 mg/L rhamnose. Aspergillus terreus KJ829487 obtained from cassava peels have the ability to produce high concentration cellulases and xylanases which effectively hydrolysed the lignocelluloses’ biomass to fermentable sugars.