[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic character...[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic characters of the new line and the control II you 838 and Shanyou 63 were comparatively studied,and storage durability of the new line and indica,japonica or hybrid rice were also comparatively analyzed by the accelerated ageing test.[Result] Agronomic characters of the new line were excellent,and germination rate in the artificial accelerated ageing test was significantly higher than those in other materials.[Conclusion] Chucangyou No.1 has the better storage durability,which can be used in large scale of extention.展开更多
On May 26, 2009, Gemdale Plaza upgraded its convenient traffic network by connecting to Dawanglu Station on Subway Line 1 in Beijing. The shopping center at Gemdale Plaza provides easy access to the subway transport s...On May 26, 2009, Gemdale Plaza upgraded its convenient traffic network by connecting to Dawanglu Station on Subway Line 1 in Beijing. The shopping center at Gemdale Plaza provides easy access to the subway transport system.Located at the junction of Changan Avenue and CBD Commercial Circle, the link to subway transport will provide a major lift to the value of Gemdale Plaza.展开更多
The aim of this study was to evaluate the effects of low concentrations of DEHP and MEHP on steroidogenesis in a murine Leydig tumor cell line （MLTC-1） in vitro. The result of flow cytometry analysis revealed that t...The aim of this study was to evaluate the effects of low concentrations of DEHP and MEHP on steroidogenesis in a murine Leydig tumor cell line （MLTC-1） in vitro. The result of flow cytometry analysis revealed that the proportion of apoptotic cells was significantly increased after the exposure to DEHP. All three genes （P450scc, P450c17, and 38HSD） under study showed an increased expression following exposure to DEHP or MEHP, although some insignificant inhibitory effects appeared in the 10μmol/L treatment group as compared with the controls. It was also found that DEHP or MEHP stimulated INSL3 mRNA and protein especially in the 0.001 μmol/L treatment group. Testosterone secretions were stimulated after the exposure to DEHP or MEHP. Alterations of steroidogenic enzymes and INSL3 in MLTC-1 cells might be involved in the biphasic effects of DEHP/MEHP on androgen production.展开更多
AIM： To analyze the expression profiles of a human gastriccancer-related gene, GCRG123, in human gastric signetring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.METHODS： In situ hybridiz...AIM： To analyze the expression profiles of a human gastriccancer-related gene, GCRG123, in human gastric signetring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.METHODS： In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues. Online software, including BLAST, Multalin and BLAT, were applied for bioinformatics analysis. National Center for Biotechnology Information （NCBI） and the University of California Santa Cruz （UCSC） databases were used for the analyses.RESULTS： The in situ hybridization signal appeared as blue precipitates restricted to the cytoplasm. Ten out of 15 cases of gastric signet ring cell carcinoma, normal gastric mucosal epithelium and pyloric glands showed high GCRG123 expression. Low GCRG123 expression was observed in gastric intestinal-type adenocarcinoma and normal gastric glands. Northern blotting revealed that GCRG123 was up-regulated in signet-ring cell carcinoma tissue but down-regulated in intestinal-type adenocarcinoma tissue. BLAST and Multalin analyses revealed that the GCRG123 sequence had 92% similarity with the ORF2 sequence of human long interspersed nuclear element retrotransposons （LINE-1, L1）. BLAT analysis indicated that GCRG123 mapped to all chromosomes. GCRG123 was found to integrate in the intron-17 and -23 of Rb, 5＇ flanking region of IL-2 and clotting factor Ⅸ genes.CONCLUSION： GCRG123, an active member of the Lt family, was up-regulated in human gastric signet-ring cell carcinoma.展开更多
基金Support by National Key Technology R&D Program(2006BAD01A01-5)Science and Technological Fund of Anhui Province for Outstanding Youth(08040106802)~~
文摘[Objective] The aim of this study was to investigate the storage durability of a new line of endurable storage hybrid rice Chucangyou No.1 selected by endurable storage genes of Yunhui 290.[Method] Agronomic characters of the new line and the control II you 838 and Shanyou 63 were comparatively studied,and storage durability of the new line and indica,japonica or hybrid rice were also comparatively analyzed by the accelerated ageing test.[Result] Agronomic characters of the new line were excellent,and germination rate in the artificial accelerated ageing test was significantly higher than those in other materials.[Conclusion] Chucangyou No.1 has the better storage durability,which can be used in large scale of extention.
文摘On May 26, 2009, Gemdale Plaza upgraded its convenient traffic network by connecting to Dawanglu Station on Subway Line 1 in Beijing. The shopping center at Gemdale Plaza provides easy access to the subway transport system.Located at the junction of Changan Avenue and CBD Commercial Circle, the link to subway transport will provide a major lift to the value of Gemdale Plaza.
基金supported by the National Natural Science Foundation of China(No.81273028)
文摘The aim of this study was to evaluate the effects of low concentrations of DEHP and MEHP on steroidogenesis in a murine Leydig tumor cell line （MLTC-1） in vitro. The result of flow cytometry analysis revealed that the proportion of apoptotic cells was significantly increased after the exposure to DEHP. All three genes （P450scc, P450c17, and 38HSD） under study showed an increased expression following exposure to DEHP or MEHP, although some insignificant inhibitory effects appeared in the 10μmol/L treatment group as compared with the controls. It was also found that DEHP or MEHP stimulated INSL3 mRNA and protein especially in the 0.001 μmol/L treatment group. Testosterone secretions were stimulated after the exposure to DEHP or MEHP. Alterations of steroidogenic enzymes and INSL3 in MLTC-1 cells might be involved in the biphasic effects of DEHP/MEHP on androgen production.
基金the Key Project from the 10th Five-year Plan of Chinese PLA, No. 01Z035International Cooperation Project from the 11th Five-year Plan of Chinese PLA, No. 06H044
文摘AIM： To analyze the expression profiles of a human gastriccancer-related gene, GCRG123, in human gastric signetring cell carcinoma tissues, and to perform bioinformatics analysis on GCRG123.METHODS： In situ hybridization was used to explore the GCRG123 expression pattern in paraffin-embedded gastric tissues, including 15 cases of signet-ring cell carcinoma, 15 of intestinal-type adenocarcinoma, and 15 of normal gastric mucosa. Northern blotting was used to analyze the differences in GCRG123 expression between stomach signet-ring cell carcinoma and intestinal-type adenocarcinoma tissues. Online software, including BLAST, Multalin and BLAT, were applied for bioinformatics analysis. National Center for Biotechnology Information （NCBI） and the University of California Santa Cruz （UCSC） databases were used for the analyses.RESULTS： The in situ hybridization signal appeared as blue precipitates restricted to the cytoplasm. Ten out of 15 cases of gastric signet ring cell carcinoma, normal gastric mucosal epithelium and pyloric glands showed high GCRG123 expression. Low GCRG123 expression was observed in gastric intestinal-type adenocarcinoma and normal gastric glands. Northern blotting revealed that GCRG123 was up-regulated in signet-ring cell carcinoma tissue but down-regulated in intestinal-type adenocarcinoma tissue. BLAST and Multalin analyses revealed that the GCRG123 sequence had 92% similarity with the ORF2 sequence of human long interspersed nuclear element retrotransposons （LINE-1, L1）. BLAT analysis indicated that GCRG123 mapped to all chromosomes. GCRG123 was found to integrate in the intron-17 and -23 of Rb, 5＇ flanking region of IL-2 and clotting factor Ⅸ genes.CONCLUSION： GCRG123, an active member of the Lt family, was up-regulated in human gastric signet-ring cell carcinoma.
