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Utilizing Dominant Early Maturity Genes of Sterile Line UP-3s in Hybrid Rice Breeding to Avoid High Temperature Season 被引量:1
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作者 Bihu Huang Zongbu Yan 《American Journal of Plant Sciences》 2015年第16期2596-2602,共7页
A new sterile line UP-3s, which carries the Dominant Early Maturity Gene (DEMG), was bred on the farm of University of Arkansas at Pine Bluff (UAPB). UP-3s and two check sterile lines, Jin23-A and Xie-A which do not c... A new sterile line UP-3s, which carries the Dominant Early Maturity Gene (DEMG), was bred on the farm of University of Arkansas at Pine Bluff (UAPB). UP-3s and two check sterile lines, Jin23-A and Xie-A which do not carry the Dominant Early Maturity Gene, were crossed with a group of different maturity restorer lines, PB-1R, PB-5R,PB11, PB-13R, PB-20, PB-21, PB-22R, and PB-23R. Eighteen new hybrid rice combinations of these crosses were then tested at UAPB in 2012 and 2013. The results showed that panicle differentiation (PD) of hybrids from female parent UP-3s (DEMG) crossed with the 8 male parents, were earlier than the hybrids from female parent Jin23-A or Xie-A crossed with the 8 male parents. The PD of these earlier hybrids was before Jun 25 and heading was before July 20. Early PD and heading avoided the high temperature (over 34&degC) period which usually occurs after July 20 in Arkansas. The yields of these earlier maturity hybrids with female parent UP-3s were higher than those of the late maturity hybrids thatwereF1 progeny of sterile lines Jin23-A or Xie-A (these two female parent checks with non-DEMG). These results showed that the DEMG sterile line UP-3s can be adopted in making crosses with later maturity restorer lines to obtain earlier maturity hybrids to avoid the high temperature period in Arkansas. 展开更多
关键词 EARLIER MATURITY DOMINANT Gene STERILE line Hybrid Rice Utilization BREEDING Avoid High Temperature
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The Protection against Lightning of an Overhead Line Uprated from 225 kV to 400 kV 被引量:1
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作者 Alain Xemard Sebastien Dennetiere +6 位作者 Ivo Uglesic Viktor Milardic Bosko Milesevic Philippe Grand Frangois Sauvegrain Pierre Stevenin Miroslav Mesic 《Journal of Energy and Power Engineering》 2012年第2期267-275,共9页
The voltage uprating of existing overhead lines is an interesting solution for increasing the transmission of electricity, especially in areas where it is difficult to build new lines. If a line is uprated with minor ... The voltage uprating of existing overhead lines is an interesting solution for increasing the transmission of electricity, especially in areas where it is difficult to build new lines. If a line is uprated with minor changes of its design and without improvement of the grounding electrodes of towers, its lightning performance remains unchanged. The consequence is that the uprated line will have a lightning flashover rate very high compared to an overhead line of same nominal voltage but of standard design. One attractive solution to solve this problem is to use line arresters. The goal of this paper is to study the use of line arresters to achieve a satisfactory lightning performance of an overhead line uprated from 225 kV to 400 kV without major design modifications. In order to compare different strategies of line arrester installation the flashover rate is calculated based on a software able to launch automatically EMTP-RV. The software named LIPS has been developed under the cover of a partnership between Hydro-Quebec, RTE and EDF. 展开更多
关键词 LIGHTNING transmission line voltage uprating insulation coordination.
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Learning Epipolar Line Window Attention for Stereo Image Super-Resolution Reconstruction
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作者 Xue Li Hongying Zhang +1 位作者 Zixun Ye Xiaoru 《Computers, Materials & Continua》 SCIE EI 2024年第2期2847-2864,共18页
Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not... Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not consider the offset of pixels along the epipolar lines in complementary views when integrating stereo information.To address these challenges,this paper introduces a novel epipolar line window attention stereo image super-resolution network(EWASSR).For detail feature restoration,we design a feature extractor based on Transformer and convolutional neural network(CNN),which consists of(shifted)window-based self-attention((S)W-MSA)and feature distillation and enhancement blocks(FDEB).This combination effectively solves the problem of global image perception and local feature attention and captures more discriminative high-frequency features of the image.Furthermore,to address the problem of offset of complementary pixels in stereo images,we propose an epipolar line window attention(EWA)mechanism,which divides windows along the epipolar direction to promote efficient matching of shifted pixels,even in pixel smooth areas.More accurate pixel matching can be achieved using adjacent pixels in the window as a reference.Extensive experiments demonstrate that our EWASSR can reconstruct more realistic detailed features.Comparative quantitative results show that in the experimental results of our EWASSR on the Middlebury and Flickr1024 data sets for 2×SR,compared with the recent network,the Peak signal-to-noise ratio(PSNR)increased by 0.37 dB and 0.34 dB,respectively. 展开更多
关键词 Stereo SR epipolar line window attention feature distillation
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Evolution Mechanism of a Severe Squall Line Triggered by the Coupling of a Sea Breeze Front and a Gust Front
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作者 万夫敬 孙敏 +2 位作者 庞华基 赵海军 赵传湖 《Journal of Tropical Meteorology》 SCIE 2024年第2期200-210,共11页
In the present study, a severe squall line(SL) was analyzed by using intensive observational surface data and radar monitoring products. In this process, mesoscale convergence lines, such as the sea breeze front(SBF),... In the present study, a severe squall line(SL) was analyzed by using intensive observational surface data and radar monitoring products. In this process, mesoscale convergence lines, such as the sea breeze front(SBF), gust front and dry line, served as the main triggering and strengthening factors. The transition from convection triggering to the formation of the initial shape was mainly affected by the convergence line of the SBF, which combined with thermal convection to form the main parts of the SL. In the later stage, the convergence line of the gust front merged with other convergence lines to form a series of strong convective cells. The SBF had good indicative significance in terms of severe convective weather warnings. The suitable conditions of heat, water vapor and vertical wind shear on the Shandong Peninsula were beneficial to the maintenance of the SL. Before SL occurrence, tropopause folding strengthened, which consequently enhanced the baroclinic property in the middle and upper troposphere. The high sensible heat flux at the surface easily produced a positive potential vorticity anomaly in the low layer, resulting in convective instability, which was conducive to the maintenance of these processes. In the system, when precipitation particles passed through the unsaturated air layer, they underwent strong evaporation, melting or sublimation, and the cooling effect formed negative buoyancy, which accelerated the sinking of the air and promoted the sustained development of the surface gale. Together with the development of lowlevel mesocyclones, the air pressure decreased rapidly, which was conducive to gale initiation. 展开更多
关键词 squall line sea breeze front gust front cold pool thunderstorm gale
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Experimental,Numerical,and Analytical Studies on the Bending of Mechanically Lined Pipe 被引量:1
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作者 WEI Wen-bin YUAN Lin +1 位作者 ZHOU Jia-sheng LIU Zheng 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期221-232,共12页
Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which cau... Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which causes the liner to separate from the outer pipe and buckles,affecting the stability of the whole line.In this paper,the buckling response of MLP subjected to bending is investigated to clarify its bending characteristics by employing both experiments,numerical simulation,as theoretical methods.Two types of MLPs were manufactured with GB 45 carbon steel(SLP)and Al 6061(ALP)used as the outer pipe material,respectively.The hydraulic expansion and bending experiments of small-scale MLPs are conducted.In addition to the ovalized shape of the cross-section for the SLP specimens,the copper liner was found to wrinkle on the compressive side.In contrast,the liner of ALP remains intact without developing any wrinkling and collapse mode.In addition,a dedicated numerical framework and theoretical models were also established.It was found both the manufacturing and bending responses of the MLP can be well reproduced,and the predicted maximum moment and critical curvatures are in good agreement with the experimental results. 展开更多
关键词 lined pipe BENDING nonlinear ring theory BUCKLING PLASTICITY
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Saturated absorption spectrum of cesium micrometric-thin cell with suppressed crossover spectral lines
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作者 韩俊龙 王博闻 +5 位作者 郑俊鹤 陈书源 肖伟 吴腾 郭弘 彭翔 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第7期353-360,共8页
Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimental... Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity. 展开更多
关键词 micrometric-thin cells saturated absorption spectrum hyperfine Zeeman splitting crossover spectral lines laser frequency stabilization
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Modeling and Simulation of a Transmission Line Response to a 400 kV/400V Capacitor Coupled Substation
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作者 Sinqobile Wiseman Nene Bolanle Tolulope Abe Agha Francis Nnachi 《Journal of Power and Energy Engineering》 2023年第12期1-14,共14页
The access to electricity in rural areas is extremely limited, but it is crucial for all citizens. The population in rural areas of sub-Saharan African (SSA) countries is generally low, making it economically unfeasib... The access to electricity in rural areas is extremely limited, but it is crucial for all citizens. The population in rural areas of sub-Saharan African (SSA) countries is generally low, making it economically unfeasible to implement traditional rural electrification (CRE) projects due to the high cost of establishing the necessary distribution infrastructure. To address this cost issue, one alternative technology for rural electrification (URE) that can be explored is the Capacitor Coupled Substation (CCS) technology. CCS is a cost-effective solution for supplying electricity to rural areas. The research is necessitated by the need to offer a cost-effective technology for supplying electricity to sparsely populated communities. This paper examines the impact on the transmission network when a 400 kV/400V CCS is connected to it. The system response when a CCS is connected to the network was modeled using MATLAB/Si-mulink. The results, based on the fixed load of 80 kW, showed negligible interference on the transmission line voltage. However, there was minor impact on the parameters downstream of the tapping point. These findings were further supported by introducing a fault condition to the CCS, which showed that interferences with the CCS could affect the overall stability of the transmission network downstream of the tapping node, similar to the behavior of an unstable load. 展开更多
关键词 Capacitor Coupled Substation Conventional Rural Electrification Unconventional Rural Electrification Transmission line Behavior Power System Simulation
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Toward a Direct Measurement of the Cosmic Acceleration:The Pilot Observation of HI 21 cm Absorption Line at FAST
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作者 Jiangang Kang Chang-Zhi Lu +1 位作者 Tong-Jie Zhang Ming Zhu 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第7期47-55,共9页
This study presents results on detecting neutral atomic hydrogen(H I)21 cm absorption in the spectrum of PKS PKS1413+13 at redshift z=0.24670041.The observation was conducted by FAST,with a spectral resolution of10 Hz... This study presents results on detecting neutral atomic hydrogen(H I)21 cm absorption in the spectrum of PKS PKS1413+13 at redshift z=0.24670041.The observation was conducted by FAST,with a spectral resolution of10 Hz,using 10 minutes of observing time.The global spectral profile is examined by modeling the absorption line using a single Gaussian function with a resolution of 10 kHz within a 2 MHz bandwidth.The goal is to determine the rate of the latest cosmic acceleration by directly measuring the redshift evolution of the H I 21 cm absorption line with Hubble flow toward a common background quasar over a decade or longer time span.This will serve as a detectable signal generated by the accelerated expansion of the Universe at redshift z<1,referred to as redshift drift z(5)or the SL effect.The measured H I gas column density in this DLA system is approximately equivalent to the initial observation value,considering uncertainties of the spin temperature of a spiral host galaxy.The high signal-to-noise ratio of 57,obtained at a 10 kHz resolution,strongly supports the feasibility of using the H I 21 cm absorption line in DLA systems to accurately measure the redshift drift rate at a precision level of around 10~(-10)per decade. 展开更多
关键词 (cosmology)cosmological parameters-cosmology observations-cosmology theory-(cosmology)dark energy-radio lines ISM-radio lines galaxies-ISM clouds
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Derivative Spectroscopy and its Application at Detecting the Weak Emission/Absorption Lines
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作者 Lihuan Yu Jiangdan Li +4 位作者 Jinliang Wang Jiajia Li Jiao Li Qiang Xi Zhanwen Han 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第3期205-223,共19页
The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened ... The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line. 展开更多
关键词 line identification-line profiles-methods data analysis-techniques spectroscopic-techniques radial velocities
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Observation of parabolic electron bands on superconductor LaRu_(2)As_(2)
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作者 周兴泰 李更 +5 位作者 潘禄禄 陈子超 李萌 时延昊 杨海涛 高鸿钧 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第7期60-64,共5页
Ru-based superconductor LaRu_(2)As_(2) has been discovered exhibiting the highest critical temperature of ~ 7.8 K among iron-free transition metal pnictides with the ThCr_(2)Si_(2)-type crystal structure. However, mic... Ru-based superconductor LaRu_(2)As_(2) has been discovered exhibiting the highest critical temperature of ~ 7.8 K among iron-free transition metal pnictides with the ThCr_(2)Si_(2)-type crystal structure. However, microscopic research on this novel superconducting material is still lacking. Here, we utilize scanning tunneling microscopy/spectroscopy to uncover the superconductivity and surface structure of LaRu_(2)As_(2). Two distinct terminating surfaces are identified on the cleaved crystals, namely, the As surface and the La surface. Atomic missing line defects are observed on the La surface. Both surfaces exhibit a superconducting gap of ~ 1.0 me V. By employing quasiparticle interference techniques, we observe standing wave patterns near the line defects on the La atomic plane. These patterns are attributed to quasiparticle scattering from two electron type parabolic bands. 展开更多
关键词 SupERCONDUCTIVITY line defect quasi-particle scattering electron band
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Evaluation of red soil-bentonite mixtures for compacted clay liners
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作者 A.S.Devapriya T.Thyagaraj 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期697-710,共14页
Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are u... Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are used for the construction of compacted clay liners if they satisfy the design criteria.However,not all soils in their natural state satisfy all the design criteria for the liner materials.Thus,there is a definite need to modify the locally available natural soils by blending with bentonite to meet the required design criteria for the liners.In view of this,the present study evaluates the suitability of an Indian red soil enhanced with bentonite as a liner material.To achieve this,a series of experiments were carried out using locally available red soil and bentonite.First,the suitability of the red soil was evaluated as a liner material.The experimental results showed that the red soil met all the selection criteria stipulated by the Environmental Protection Agencies(EPAs)for the liners except the hydraulic conductivity criterion.Therefore,the red soil was mixed with bentonite contents of 10%,20%and 30%,and the red soil-bentonite mixtures were evaluated for their suitability for liners in their compacted state.Further,as the liners in the arid and semi-arid regions are subjected to moisture variations due to seasonal moisture fluctuations and other factors,the red soil-bentonite mixtures were subjected to wetdry cycles,and their suitability was evaluated after wet-dry cycles.The experimental results revealed that all the red soil-bentonite mixtures met the stipulated EPA criteria for the liners in the as-compacted state.However,the red soil-bentonite mixtures with 20%and 30%bentonite contents only satisfied the hydraulic conductivity requirement even after wet-dry cycles.The experimental findings were supplemented with the microstructural insights captured through digital camera images,scanning electron microscopy(SEM),and mercury intrusion porosimetry(MIP)studies. 展开更多
关键词 Compacted clay lines Hydraulic conductivity Wet-dry cycles Microstructure
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ST-LaneNet: Lane Line Detection Method Based on Swin Transformer and LaneNet
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作者 Yufeng Du Rongyun Zhang +3 位作者 Peicheng Shi Linfeng Zhao Bin Zhang Yaming Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第1期130-145,共16页
The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line dete... The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line detection have been proposed by researchers in the field.However,owing to the simple appearance of lane lines and the lack of distinctive features,it is easy for other objects with similar local appearances to interfere with the process of detecting lane lines.The precision of lane line detection is limited by the unpredictable quantity and diversity of lane lines.To address the aforementioned challenges,we propose a novel deep learning approach for lane line detection.This method leverages the Swin Transformer in conjunction with LaneNet(called ST-LaneNet).The experience results showed that the true positive detection rate can reach 97.53%for easy lanes and 96.83%for difficult lanes(such as scenes with severe occlusion and extreme lighting conditions),which can better accomplish the objective of detecting lane lines.In 1000 detection samples,the average detection accuracy can reach 97.83%,the average inference time per image can reach 17.8 ms,and the average number of frames per second can reach 64.8 Hz.The programming scripts and associated models for this project can be accessed openly at the following GitHub repository:https://github.com/Duane 711/Lane-line-detec tion-ST-LaneNet. 展开更多
关键词 Autonomous driving Lane line detection Deep learning Swin transformer
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Optimizing profile line interval for enhanced accuracy in rock joint morphology and shear strength assessments
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作者 Leibo Song Quan Jiang +5 位作者 Shigui Du Jiamin Song Gang Wang Yanting Gu Xingkai Wang Jinzhong Wu 《International Journal of Mining Science and Technology》 SCIE EI CAS CSCD 2024年第5期587-608,共22页
2D profile lines play a critical role in cost-effectively evaluating rock joint properties and shear strength.However, the interval(ΔI_(L)) between these lines significantly impacts roughness and shear strength asses... 2D profile lines play a critical role in cost-effectively evaluating rock joint properties and shear strength.However, the interval(ΔI_(L)) between these lines significantly impacts roughness and shear strength assessments. A detailed study of 45 joint samples using four statistical measures across 500 different ΔI_(L)values identified a clear line interval effect with two stages: stable and fluctuation-discrete.Further statistical analysis showed a linear relationship between the error bounds of four parameters,shear strength evaluation, and their corresponding maximum ΔI_(L)values, where the gradient k of this linear relationship was influenced by the basic friction angle and normal stress. Accounting for these factors,lower-limit linear models were employed to determine the optimal ΔI_(L)values that met error tolerances(1%–10%) for all metrics and shear strength. The study also explored the consistent size effect on joints regardless of ΔI_(L)changes, revealing three types of size effects based on morphological heterogeneity.Notably, larger joints required generally higher ΔI_(L)to maintain the predefined error limits, suggesting an increased interval for large joint analyses. Consequently, this research provides a basis for determining the optimal ΔI_(L), improving accuracy in 2D profile line assessments of joint characteristics. 展开更多
关键词 Rock joint ROUGHNESS Shear strength Size effect Profile line interval effect
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An Implementation of Multiscale Line Detection and Mathematical Morphology for Efficient and Precise Blood Vessel Segmentation in Fundus Images
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作者 Syed Ayaz Ali Shah Aamir Shahzad +4 位作者 Musaed Alhussein Chuan Meng Goh Khursheed Aurangzeb Tong Boon Tang Muhammad Awais 《Computers, Materials & Continua》 SCIE EI 2024年第5期2565-2583,共19页
Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when deal... Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when dealing with color fundus images due to issues like non-uniformillumination,low contrast,and variations in vessel appearance,especially in the presence of different pathologies.Furthermore,the speed of the retinal vessel segmentation system is of utmost importance.With the surge of now available big data,the speed of the algorithm becomes increasingly important,carrying almost equivalent weightage to the accuracy of the algorithm.To address these challenges,we present a novel approach for retinal vessel segmentation,leveraging efficient and robust techniques based on multiscale line detection and mathematical morphology.Our algorithm’s performance is evaluated on two publicly available datasets,namely the Digital Retinal Images for Vessel Extraction dataset(DRIVE)and the Structure Analysis of Retina(STARE)dataset.The experimental results demonstrate the effectiveness of our method,withmean accuracy values of 0.9467 forDRIVE and 0.9535 for STARE datasets,aswell as sensitivity values of 0.6952 forDRIVE and 0.6809 for STARE datasets.Notably,our algorithmexhibits competitive performance with state-of-the-art methods.Importantly,it operates at an average speed of 3.73 s per image for DRIVE and 3.75 s for STARE datasets.It is worth noting that these results were achieved using Matlab scripts containing multiple loops.This suggests that the processing time can be further reduced by replacing loops with vectorization.Thus the proposed algorithm can be deployed in real time applications.In summary,our proposed system strikes a fine balance between swift computation and accuracy that is on par with the best available methods in the field. 展开更多
关键词 line detector vessel detection LOCALIZATION mathematical morphology image processing
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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines
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作者 Justus Thomas Obiajulu Sievers Anom Bowolaksono R.Tedjo Sasmono 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第5期215-224,共10页
Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,J... Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery. 展开更多
关键词 Zika virus Infectivity REPLICATION Cell lines In vitro
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Polar-coordinate line-projection light-curing continuous 3D printing for tubular structures
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作者 Huiyuan Wang Siqin Liu +12 位作者 Xincheng Yin Mingming Huang Yanzhe Fu Xun Chen Chao Wang Jingyong Sun Xin Yan Jianmin Han Jiping Yang Zhijian Wang Lizhen Wang Yubo Fan Jiebo Li 《International Journal of Extreme Manufacturing》 SCIE EI CAS CSCD 2024年第4期247-260,共14页
3D printing techniques offer an effective method in fabricating complex radially multi-material structures.However,it is challenging for complex and delicate radially multi-material model geometries without supporting... 3D printing techniques offer an effective method in fabricating complex radially multi-material structures.However,it is challenging for complex and delicate radially multi-material model geometries without supporting structures,such as tissue vessels and tubular graft,among others.In this work,we tackle these challenges by developing a polar digital light processing technique which uses a rod as the printing platform.The 3D model fabrication is accomplished through line projection.The rotation and translation of the rod are synchronized to project and illuminate the photosensitive material volume.By controlling the distance between the rod and the printing window,we achieved the printing of tubular structures with a minimum wall thickness as thin as 50 micrometers.By controlling the width of fine slits at the printing window,we achieved the printing of structures with a minimum feature size of 10 micrometers.Our process accomplished the fabrication of thin-walled tubular graft structure with a thickness of only 100 micrometers and lengths of several centimeters within a timeframe of just 100 s.Additionally,it enables the printing of axial multi-material structures,thereby achieving adjustable mechanical strength.This method is conducive to rapid customization of tubular grafts and the manufacturing of tubular components in fields such as dentistry,aerospace,and more. 展开更多
关键词 3D printing polar coordinate line projection LIGHT-CURING tubular structure radially multi-material structures
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Analysis of mechanical strengths of extreme line casing joint considering geometric, material, and contact nonlinearities
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作者 Ji-Yun Zhang Chi Peng +4 位作者 Jian-Hong Fu Quan Cao Yu Su Jian-Yun Pang Zi-Qiang Yu 《Petroleum Science》 SCIE EI CAS CSCD 2024年第3期1992-2004,共13页
To address the challenges associated with difficult casing running,limited annular space,and poor cementing quality in the completion of ultra-deep wells,the extreme line casing offers an effective solution over conve... To address the challenges associated with difficult casing running,limited annular space,and poor cementing quality in the completion of ultra-deep wells,the extreme line casing offers an effective solution over conventional casings.However,due to its smaller size,the joint strength of extreme line casing is reduced,which may cause failure when running in the hole.To address this issue,this study focuses on the CST-ZTΦ139.7 mm×7.72 mm extreme line casing and employs the elastic-plastic mechanics to establish a comprehensive analysis of the casing joint,taking into account the influence of geometric and material nonlinearities.A finite element model is developed to analyze the forces and deformations of the extreme line casing joint under axial tension and external collapse load.The model investigates the stress distribution of each thread tooth subjected to various tensile forces and external pressures.Additionally,the tensile strength and crushing strength of the extreme line casing joint are determined through both analytical and experimental approaches.The findings reveal that,under axial tensile load,the bearing surface of each thread tooth experiences uneven stress,with relatively high equivalent stress at the root of each thread tooth.The end thread teeth are valuable spots for failure.It is observed that the critical fracture axial load of thread decreases linearly with the increase of thread tooth sequence.Under external pressure,the circumferential stress is highest at the small end of the external thread,leading to yield deformation.The tensile strength of the joint obtained from the finite element model exhibits a relative error of less than 7%compared to the analytical and experimental values,proving the reliability of the finite element model.The tensile strength of the joint is 3091.9 k N.Moreover,in terms of anti-collapse capability,the joints demonstrate higher resistance to collapse compared to the casing body,which is consistent with the test results where the pipe body experiences collapse and failure while the joints remain intact during the experiment.The failure load of the casing body under external collapse pressure is 87.4 MPa.The present study provides a basic understanding of the mechanical strengths of extreme line casing joint. 展开更多
关键词 Extreme line casing Elastic-plastic mechanics Finite element analysis Tensile strength Collapse strength
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Establishment and characterization of a canine chondrosarcoma cell line:Mango
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作者 Meilin Wang Xiao Wang +8 位作者 Lixin He Hongbo Gao Wenxuan Li Huili Feng Qingyuan Zhao Wenwen Zhang Chengzong Li Bohan Zhang Changwei Qiu 《Animal Diseases》 CAS 2024年第3期186-197,共12页
In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines ha... In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines has yet to be reported.This study established a canine CSA cell line to facilitate the basic clinical study of canine CSA.Fifty fve cases of canine osteolytic disease were collected,and more than 10 bone tumor samples from dogs with typical clinical signs were used for primary cell culture.A cell line with stable passaging for more than 100 generations and mouse tumorigenic ability was successfully cultured.According to the clinical characteristics of the dog and the histopathological results of the primary tumor,CSA was diagnosed,and the CSA cell line was designated Mango.Immunohistochemical(IHC)results showed that the immunoreactivity of bone gamma-carboxyglutamate protein(BGLAP),secreted protein acidic and rich in cysteine(SPARC),alkaline phosphatase(ALPL),vimentin(VIM)and S100 were positive.However,the immunoreactivity of pan-cytokeratin(PCK),chromogranin A(CGA),and platelet endothelial cell adhesion molecule-1(CD31)was negative.Immunofuorescence(IF)results showed that the protein expressions in the Mango cell line were consistent with the IHC identifcation of the primary tumor.The Mango cell line’s doubling time was 43.92 h,and the cell formation rate exceeded 20%.There were abnormal chromosome numbers,hetero staining with toluidine blue,and certain calcifcation abilities.It could be passaged stably and continuously without changing the cell morphology and characteristics.In vivo,the cells were successfully injected into the nude mice model with a tumorigenic rate of 100%.The immunophenotype of the xenograft tumor was consistent with that of the primary tumor.Therefore,we efectively established a canine CSA cell line.As a promising cell material,this cell line can be used to construct a tumor-bearing model conducive to the subsequent basic research of canine CSA.Moreover,because of its similarity to human CSA,the animal model of CSA is also indispensable for investigating human CSA. 展开更多
关键词 Canine chondrosarcoma Cell line ESTABLISHMENT CHARACTERIZATION Tumorigenicity
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Imaging of human parafoveal area with large field of view in adaptive optics line scanning ophthalmoscope
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作者 Wen Kong Yiwei Chen +1 位作者 Guohua Shi Yi He 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2024年第6期41-51,共11页
The parafoveal area,with its high concentration of photoreceptors andfine retinal capillaries,is crucial for central vision and often exhibits early signs of pathological changes.The current adaptive optics scanning l... The parafoveal area,with its high concentration of photoreceptors andfine retinal capillaries,is crucial for central vision and often exhibits early signs of pathological changes.The current adaptive optics scanning laser ophthalmoscope(AOSLO)provides an excellent tool to acquire accurate and detailed information about the parafoveal area with cellular resolution.However,limited by the scanning speed of two-dimensional scanning,thefield of view(FOV)in the AOSLO system was usually less than or equal to 2,and the stitching for the parafoveal area required dozens of images,which was time-consuming and laborious.Unfortunately,almost half of patients are unable to obtain stitched images because of their poorfixation.To solve this problem,we integrate AO technology with the line-scan imaging method to build an adaptive optics line scanning ophthalmoscope(AOLSO)system with a larger FOV.In the AOLSO,afocal spherical mirrors in pairs are nonplanar arranged and the distance and angle between optical elements are optimized to minimize the aberrations,two cylinder lenses are orthogonally placed before the imaging sensor to stretch the point spread function(PSF)for sufficiently digitizing light energy.Captured human retinal images show the whole parafoveal area with 55FOV,60 Hz frame rate and cellular resolutions.Take advantage of the 5FOV of the AOLSO,only 9 frames of the retina are captured with several minutes to stitch a montage image with an FOV of 99,in which photoreceptor counting is performed within approximately 5eccentricity.The AOLSO system not only provides cellular resolution but also has the capability to capture the parafoveal region in a single frame,which offers great potential for noninvasive studying of the parafoveal area. 展开更多
关键词 Adaptive optics line scanning retinal imaging system parafoveal capillary vasculature.
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On traceable iterated line graph and hamiltonian path index
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作者 NIU Zhao-hong XIONG Li-ming YANG Wei-hua 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第2期239-252,共14页
Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characteri... Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characterization of G for which L^(n)(G)has a hamiltonian path.As applications,we use this characterization to give several upper bounds on the hamiltonian path index of a graph. 展开更多
关键词 iterated line graph TRACEABLE hamiltonian index hamiltonian path index
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