Bioinformatics analysis of the structure and linear B-cell epitopes of aquaporin-3 from Schistosoma japonicum

Objective:To analyze the structure of aquaporins-3(AQP-3) from Schistosoma japonicum(SJAQP-3) using bioinformalical methods,and to provid of references for vaccine targets research.Methods:Protparam,BepiPred,TMHMM Server,MLRC,Geno3d,DNA star software packages were used to predict the physical and chemical properties,hydrophilicity plot, flexibility regions,antigenic index,surface probability plot,secondary structure,and tertiary structure of amino acid sequence of SJAQP-3.Results:SJAQP-3 had six transmembrane regions and two half-spanning helices that form a central channel.The half-spanning helices fold into the centre of the channel.Either of the half-spanning helix had a conserved motif of NPA common to all aquaporins.Predicted linear B-Cell epitopes were most likely at the N-terminal amino acid residues of Saa-7aa,59aa- 62aa,225aa-230aa,282aa -288aa,294aa -29Saa and 305aa -307aa area.59aa- 62aa,22Saa-230aa located outside the membrane,the others located inside the cell.Conclusions:SJAQP-3 is a integral membrane protein in Schistosoma japonicum tegument.There are six potential epitopes in SJ AQP-3.It might be a potential molecular target for the development of vaccines.

Exact Location of Linear B-cell Epitopes of VP3 Protein of Goose Parvovirus

Four monoclonal antibodies (MAbs) against Goose Parvovirus (GPV) VP3 protein already available were used to precisely locate linear B-cell epitopes in VP3 of GPV. The epitopes, recognized by four MAbs, had already been identified at low levels of resolution. Complementary oligonucleotides encoding ten amino acid fragments, with five amino acid overlaps were designed with suitable sticky ends for recombination with pET-32a and subsequent expression as small-fragment fusion proteins. Antigenicity of specific oligopeptides was determined by Western blotting with the MAbs. Using the same methods, amino acids were deleted one by one from the peptides of interest, enabling the two epitopes to be precisely located at amino acids 430-435 (-DRIMNP-) and 643-647 (-VFIKN-).

Establishment and validation of a method for cell irradiation in 96-well and 6-well plates using a linear accelerator

To establish and validate a method for cell irradiation in 96-well and 6-well plates using a linear accelerator, three irradiation methods(G0 B0 F40,G0 B1.5 F40, and G180 B1.5 F40) were designed to irradiate cell culture plasticware simulated with RW3 slab phantom and polystyrene. The difference between the actual physical measured dose and the preset dose was compared among the three methods under the preparatory conditions of 2, 4, 6, 8, and 10 Gy. MDA-MB-231 cells were analyzed by using a cell proliferation assay and a clonogenic assay to verify the difference between the three cell irradiation methods on cell radiosensitivity. For each preset dose, the difference between the actual measured dose and the preset dose was the lowest for Method G0 B1.5 F40, the second lowest for Method G180 B1.5 F40, and the maximum for Method GOB0 F40. The ranges of the differences were-0.28 to 0.02%,-2.17 to-1.80%, and-4.92 to-4.55%, and 0.31 to-0.12%,-3.42 to-2.86%, and-7.31 to-6.92%,respectively, for 96-well and 6-well plates. The cell culture experiments proved that Method G0 B1.5 F40 was an accurate, effective, simple, and practical irradiation method. The most accurate and effective cell irradiation method should always be used, as it will reduce dose differences and instability factors and provide improved accuracy and comparability for laboratories researching cellular radiosensitivity.

Nuclear Fuel Cell Calculation Using Collision Probability Method with Linear Non Flat Flux Approach

Nuclear fuel cell calculation is one of the most complicated steps of neutron transport problems in the reactor core. A few numerical methods use neutron flat flux (FF) approximation to solve this problem. In this approach, neutron flux spectrum is assumed constant in each region. The solution of neutron transport equation using collision probability (CP) method based on non flat flux (NFF) approximation by introducing linear spatial distribution function implemented to a simple cylindrical annular cell has been carried out. In this concept, neutron flux spectrum in each region is different each other because of an existing of the spatial function. Numerical calculation of the neutron flux in each region of the cell using NFF approach shows a fairly good agreement compared to those calculated using existing SRAC code and FF approach. Moreover, calculation of the neutron flux in each region of the nuclear fuel cell using NFF approach needs only 6 meshes which give equivalent result when it is calculated using 24 meshes in FF approach. This result indicates that NFF approach is more efficient to be used to calculate the neutron flux in the regions of the cell than FF approach.

基于线性加权的Cell-ID定位方法

基于Google基站数据库,提出一种基于线性加权的Cell-ID定位方法,以综合利用智能移动终端在定位时所能接收到的小区基站的信号强度和相应的小区基站坐标等信息。该方法仅需要升级智能终端软件就可以实现定位。实验表明,在2σ要求下,定位误差小于150 m,可以满足精度要求比较低的基于位置的服务的应用需求。

初始大扰动条件下双流系统的粒子相空间振荡

双流系统产生的不稳定性过程是近地空间中常见的等离子体不稳定性现象.地球磁尾、等离子体片边界层、地球弓激波、太阳风、极隙区和极光加速区观测到的静电孤立波被认为是由双流不稳定性产生.地球空间环境并非均匀稳态,因此对双流系统演化过程的研究往往需要考虑初始的扰动.利用所建立的动理论粒子云模型方法,我们研究了初始大扰动下双流系统的不稳定性现象,发现初始大扰动条件下粒子相空间产生振荡现象,且振荡频率与初始扰动的空间波数有关,电场与电场能量也存在该频率成分.通过线性理论,求解出了粒子相空间振荡频率.它与粒子云模拟得到的频率一致.该频率为朗缪尔振荡频率的基础之上叠加一个与初始扰动的空间波数有关的频率偏移量.我们改变等离子体频率,进一步验证了上述结论.该研究结果表明初始扰动能够对双流系统的不稳定性现象产生重要影响,对于加深理解地球磁层中双流系统的演化过程有重要参考意义.

肠道病毒C组96型VP1蛋白线性B细胞表位筛选与鉴定

目的鉴定肠道病毒C组96型(enterovirus C96,EV-C96)VP1蛋白的线性B细胞表位。方法采用IEDB网站提供的在线分析工具分析、预测EV-C96 VP1蛋白的线性B细胞表位,结合其二级结构、β-转角、抗原性、亲水性等多项参数,筛选出优势候选表位;应用间接ELISA法检测候选表位与EV-C96免疫血清的反应性以及与21种其他常见手足口病病原血清的交叉反应性;应用微量中和试验测定表位抗体的中和效价;采用序列比对分析表位的序列保守性;应用结构对齐分析表位的结构特异性。结果通过生物信息学筛选出EVC96 VP1蛋白7个候选表位(P1~P7);ELISA法检测发现P7(氨基酸序列位置为282~304)与EV-C96多克隆抗体有强反应性,与其他常见EV多克隆抗体不发生明显反应;微量中和试验显示,P7抗体的中和效价低于1∶2;序列及结构分析结果显示,P7的氨基酸序列在EV-C96型内具有较高保守性,与其他EV相应位点氨基酸序列的结构有明显差异。结论P7表位为EV-C96特异的非中和性B细胞表位,可作为开发EV-C96检测试剂盒的候选抗原表位。

机器人制造单元集成式工艺规划与调度方法

文章针对机器人制造单元集成式工艺规划与调度(RMC-IPPS)问题,并考虑其加工次序柔性、加工柔性及工序柔性,提出了一种多层级线性编码的方法,该方法能有效地将RMC-IPPS问题的解用简单的数据结构表示,并实现对问题的整体求解。同时,针对毛坯件/半成品在单元中的转运问题,设计了一种基于贪婪思想的机器人调度策略,该策略每一步都选择当前的最优状态,使生产周期尽量不因搬运工序的存在而产生过多延滞。最后,设计出一种基于外部档案库的NSGA-Ⅱ算法(M-NSGA-Ⅱ),该算法通过外部档案库对非劣解进行二次搜索,以避免种群多样性趋于单一,并通过基准实例与实际生产应用算例对所提方法进行验证,并将改进后的算法与其它算法进行对比。结果表明,相较标准NSGA-Ⅱ算法,M-NSGA-Ⅱ算法的覆盖率为100%;相较文献中的算法,M-NSGA-Ⅱ算法具有更高的覆盖率。

CellSearch系统对细胞检出率和线性度性能检测方法的研究

该文对Cell Search系统在细胞检出率和线性度性能检测方法进行研究,从而分析出适应Cell Search系统特征的准确合理的检测方法。

A Linear Interpolation-Based Algorithm for Path Planning and Replanning on Girds

Field D* algorithm is widely used in mobile robot navigation since it can plan and replan any-angle paths through non-uniform cost grids. However, it still suffers from inefficiency and sub-optimality. In this article, a new linear interpolation-based planning and replanning algorithm, Update-Reducing Field D*, is proposed. It employs different approaches during initial planning and replanning respectively in order to reduce the number of updates of the rhs-values of vertices. Experiments have shown that Update-Reducing Field D* runs faster than Field D* and returns smoother and lower-cost paths.

固体氧化物燃料电池的自抗扰优化控制方法研究

固体氧化物燃料电池(Solid oxide fuel cell,SOFC)因其多燃料适应性、高效能和无污染等特点而应用广泛。然而,SOFC是一个高度耦合、非线性、多变量和复杂的系统,易受扰动的影响而使系统输出电压不断波动变化。因此,为了降低扰动带来的影响,保证SOFC系统输出电压稳定,提出了一种基于改进萤火虫算法的线性自抗扰控制(Linear active disturbance rejection control,LADRC)方法,将萤火虫算法引入阶梯型惯性权重,并采用随机生成方法替代发光程度低的30%个体,进而优化LADRC参数。仿真结果表明,在电压扰动影响下,通过改进萤火虫算法优化的LADRC控制方法比传统PID控制方法的超调量降低了13.65%,具有良好的控制效果。

Non-Linear Mathematical Model of the Interaction between Tumor and Oncolytic Viruses

A mathematical modeling of tumor therapy with oncolytic viruses is discussed. The model consists of two coupled, deterministic differential equations allowing for cell reproduction and death, and cell infection. The model is one of the conceptual mathematical models of tumor growth that treat a tumor as a dynamic society of interacting cells. In this paper, we obtain an approximate analytical expression of uninfected and infected cell population by solving the non-linear equations using Homotopy analysis method (HAM). Furthermore, the results are compared with the numerical simulation of the problem using Matlab program. The obtained results are valid for the whole solution domain.

1型登革病毒感染病人血清中非结构蛋白1B细胞线性表位鉴定

目的鉴定1型登革病毒感染病人血清中1型登革病毒非结构蛋白1(nonstructural Protein 1 of Dengue virus type 1,DENV-1 NS1)B细胞线性表位。方法以重组DENV-1 NS1为抗原,免疫印迹法筛选NS1阳性病人血清。一组重叠DENV-1 NS1的重叠多肽同这些NS1阳性血清反应鉴定NS1蛋白线性B细胞表位。采用梯度稀释的1-4型重组DENVNS1竞争抑制阳性多肽同病人血清反应鉴定其反应特异性和交叉反应性。结果12份DENV-1核酸阳性病人中筛选得到5份抗DENV-1 NS1阳性病人血清。重叠多肽法、竞争抑制法和生物信息学方法筛选出3条阳性NS1多肽(氨基酸残基序列第1-15,131-145,271-285),其中aa1-15在已报道的DENV-1分离株高度保守,提示这个区域可能存在DENV-1血清型特异性表位。aa131-145和aa271-285对应的蛋白序列比对分析表明以上区域中的133-FI/LIDGP-138和271-GKLEL/M/IDF-277在已报道的4种血清型DENV分离株中高度保守,提示这2个区域存在4种血清型DENV共同表位。结论发现3个NS1上B细胞线性表位,其中aa131-145和aa271-285为首次报道,结果有助于疫苗和诊断试剂的研发。

The Effect of Shot-Peening Treatment on Microstructure and Corrosion Behavior of Closed-Cell Aluminum Foam

Closed-cell aluminum foam was shot peened at different processing time (0 s, 5 s, 10 s, and 20 s), the intensity was the 0.12 mmA. The X-ray diffraction results showed that the reflections became weakened obviously with the shot peened time increased. Combined with Popa model and lognormal distribute model, the surface microstructure of closed-cell aluminum foam was inves-tigated by using the Rietveld whole pattern fitting analysis method. The results revealed that domain size and microstrain fluctuated along different reflection directions after shot peened, which attributed to the random and anisotropic deformation direction. With the shot peened processing time prolonged, a decrease in domain size and an increase in microstrain were also observed. Moreover, the corrosion behavior of closed-cell aluminum foam was studied by weight-loss test. The results indicated that corrosion properties of specimen subjected to shot peened processing was better than the unpeened specimens.

Approximate Analytical Expressions for the Concentrations of Acetate and Methane in the Microbial Electrochemical Cell

Mathematical modeling of microbial electrochemical cells (MXCs) for both microbial fuel cell and microbial electrolysis cell is discussed. The model is based on the system of reaction diffusion of reaction-diffusion equation containing a non-linear term related to substrate consumption rates by electrogeneic and methanogenic microorganism in the bioflim. This paper presents the approximate analytical method to solve the non-linear differential equation that describes the diffusion coupled with acetate (substrate) consumption rates. Simple analytical expressions for the concentrations of acetate and methane have been derived for all experimental values of bulk concentration, distributions of microbial volume fraction, local potential in the biofilm and biofilm thickness. In addition, sensitivity of the parameters on concentrations is also discussed. Our analytical results are also validated with the numerical results and limiting cases results. Further, a graphical procedure for estimating the kinetic parameters is also suggested.

鸡蛋过敏原Gal d 4线性表位的预测定位及鉴定

采用生物信息学软件GenBank数据库、PDB数据库、Immunomedicine Group、Bepipred-2.0 server、ABCpred、NetMHCⅡpan 4.0 Serer、SYFPEITHI、NetMHC和NetCTL在线工具分析鸡蛋过敏原溶菌酶Gal d 4的一级氨基酸序列、二级结构并预测其B细胞线性表位和T细胞表位。利用溶菌酶三级结构空间模型,对预测的线性表位进行定位。同时,利用鸡蛋过敏患者血清对Gal d 4的B细胞线性表位进行鉴定。结果表明,3种生物信息学软件预测的5个相同潜在B细胞线性表位为:19 KVFGRCELAAAMKR 32、57 NTQA 60、65 TDG 67、87 TPGSRNLCNI 96和132 RCKGTDVQAWI 142。此外发现,19 KVFGRCELAAAMKR 32、57 NTQA 60和132 RCKGTDVQAWI 142、65 TDG 67和87 TPGSRNLCNI 96在氨基酸序列上不连续,却在空间结构上邻近,因此,这部分线性表位可能共同构成构象表位。预测所得2个T细胞抗原表位分别为:42 SLGNWVCAA 50和100 ALLSSDITA 108。经鉴定所得具有免疫活性的B细胞线性表位为:19 KVFGRCELAAAMKR 32。该研究首次对鸡蛋过敏原Gal d 4的B细胞线性表位和T细胞表位预测定位及鉴定,有助于进一步认识鸡蛋过敏原Gal d 4的功能与结构,对鸡蛋过敏原的识别与检测具有重要的参考与借鉴作用。

燃料电池空气系统非奇异滑模控制

为了提高燃料电池的效率和使用寿命,必须对其多个状态进行精确的控制。首先,建立了面向控制的燃料电池空气系统四阶非线性方程,并进行模型有效性验证。然后,针对空气供给系统压力和空气流量的非线性和强耦合性等特点,提出了一种基于全局反馈线性化理论的非奇异终端滑模控制策略。反馈线性化通过对空压机转速和背压阀开度的协同控制,将非线性模型转化为线性模型,实现对阴极压力和空气流量的解耦;考虑空气系统在复杂环境下受到不确定性扰动,设计比例积分观测器对扰动进行观测以减少环境影响;在此基础上,设计非奇异滑模控制器。仿真结果表明:非奇异滑模控制的阴极压力和过氧比各误差积分均小于传统滑模和反馈线性化控制,可显著提高燃料电池空气供给控制系统精度和鲁棒性,对今后研发高精度燃料电池阴极空气供给控制系统提供参考。

强保持传递布尔矩阵的线性算子

该文研究强保持传递布尔矩阵的线性算子,证明了这类算子将细胞仍变为细胞(细胞是仅有一个非零元的布尔矩阵),从而必为双射.

自制高浓度样本对全自动血细胞分析仪的线性验证及评价

目的自制高浓度血液样本对MEK-7300P全自动血细胞分析仪进行线性范围的验证。方法富集多份日常健康献血者血常规标本中的血细胞制成高浓度样本,参照国家卫生行业标准WS/T 406-2012、WS/T 408-2012和仪器说明书要求,运用2种方法对MEK-7300P全自动血液细胞分析仪的线性范围进行验证。结果自制高浓度血液样本的红细胞(red blood cell,RBC)、血红蛋白(hemoglobin,Hb)、红细胞压积(hematocrit,HCT)、白细胞(leukocyte,WBC)和血小板(platelet,PLT)5个项目均通过线性范围的验证,均为一阶线性,且验证范围可覆盖99.9%的日常检测范围。RBC、Hb、HCT、WBC和PLT线性验证数据的不精密度评估值分别为3.10%、3.50%、2.94%、2.55%和1.86%,均小于判定界值8.45%,数据的精密度良好,满足线性评价的要求。结论富集日常血常规标本的血细胞样本,可用于验证MEK-7300P全自动血细胞分析仪的线性范围,可满足血站日常工作需求。