Objective:To analyze the structure of aquaporins-3(AQP-3) from Schistosoma japonicum(SJAQP-3) using bioinformalical methods,and to provid of references for vaccine targets research.Methods:Protparam,BepiPred,TMHMM Ser...Objective:To analyze the structure of aquaporins-3(AQP-3) from Schistosoma japonicum(SJAQP-3) using bioinformalical methods,and to provid of references for vaccine targets research.Methods:Protparam,BepiPred,TMHMM Server,MLRC,Geno3d,DNA star software packages were used to predict the physical and chemical properties,hydrophilicity plot, flexibility regions,antigenic index,surface probability plot,secondary structure,and tertiary structure of amino acid sequence of SJAQP-3.Results:SJAQP-3 had six transmembrane regions and two half-spanning helices that form a central channel.The half-spanning helices fold into the centre of the channel.Either of the half-spanning helix had a conserved motif of NPA common to all aquaporins.Predicted linear B-Cell epitopes were most likely at the N-terminal amino acid residues of Saa-7aa,59aa- 62aa,225aa-230aa,282aa -288aa,294aa -29Saa and 305aa -307aa area.59aa- 62aa,22Saa-230aa located outside the membrane,the others located inside the cell.Conclusions:SJAQP-3 is a integral membrane protein in Schistosoma japonicum tegument.There are six potential epitopes in SJ AQP-3.It might be a potential molecular target for the development of vaccines.展开更多
目前，宝钢集团八钢公司第一座256烧结系统中，工控系统中HMI（人机界面）使用的是西门子的产品wINCC。在实际操作过程中，操作员的操作失误引起设备故障的追诉，存在着缺失记录的情况，无法在时间和过程中来分析原因和采取规避措施。...目前，宝钢集团八钢公司第一座256烧结系统中，工控系统中HMI（人机界面）使用的是西门子的产品wINCC。在实际操作过程中，操作员的操作失误引起设备故障的追诉，存在着缺失记录的情况，无法在时间和过程中来分析原因和采取规避措施。因此．有必要对操作员的操作方法进行监控和记录，同时为操作员标准化操作起到推进作用。本文介绍了基于WINCC中v b script语言的强大功能来实现这一需求。展开更多
目的鉴定1型登革病毒感染病人血清中1型登革病毒非结构蛋白1(nonstructural Protein 1 of Dengue virus type 1,DENV-1 NS1)B细胞线性表位。方法以重组DENV-1 NS1为抗原,免疫印迹法筛选NS1阳性病人血清。一组重叠DENV-1 NS1的重叠多肽...目的鉴定1型登革病毒感染病人血清中1型登革病毒非结构蛋白1(nonstructural Protein 1 of Dengue virus type 1,DENV-1 NS1)B细胞线性表位。方法以重组DENV-1 NS1为抗原,免疫印迹法筛选NS1阳性病人血清。一组重叠DENV-1 NS1的重叠多肽同这些NS1阳性血清反应鉴定NS1蛋白线性B细胞表位。采用梯度稀释的1-4型重组DENVNS1竞争抑制阳性多肽同病人血清反应鉴定其反应特异性和交叉反应性。结果12份DENV-1核酸阳性病人中筛选得到5份抗DENV-1 NS1阳性病人血清。重叠多肽法、竞争抑制法和生物信息学方法筛选出3条阳性NS1多肽(氨基酸残基序列第1-15,131-145,271-285),其中aa1-15在已报道的DENV-1分离株高度保守,提示这个区域可能存在DENV-1血清型特异性表位。aa131-145和aa271-285对应的蛋白序列比对分析表明以上区域中的133-FI/LIDGP-138和271-GKLEL/M/IDF-277在已报道的4种血清型DENV分离株中高度保守,提示这2个区域存在4种血清型DENV共同表位。结论发现3个NS1上B细胞线性表位,其中aa131-145和aa271-285为首次报道,结果有助于疫苗和诊断试剂的研发。展开更多
In this paper, for general linear methods applied to strictly dissipative initial value problem in Hilbert spaces, we prove that algebraic stability implies B-convergence, which extends and improves the existing resul...In this paper, for general linear methods applied to strictly dissipative initial value problem in Hilbert spaces, we prove that algebraic stability implies B-convergence, which extends and improves the existing results on Runge-Kutta methods. Specializing our results for the case of multi-step Runge-Kutta methods, a series of B-convergence results are obtained.展开更多
Four monoclonal antibodies (MAbs) against Goose Parvovirus (GPV) VP3 protein already available were used to precisely locate linear B-cell epitopes in VP3 of GPV. The epitopes, recognized by four MAbs, had already bee...Four monoclonal antibodies (MAbs) against Goose Parvovirus (GPV) VP3 protein already available were used to precisely locate linear B-cell epitopes in VP3 of GPV. The epitopes, recognized by four MAbs, had already been identified at low levels of resolution. Complementary oligonucleotides encoding ten amino acid fragments, with five amino acid overlaps were designed with suitable sticky ends for recombination with pET-32a and subsequent expression as small-fragment fusion proteins. Antigenicity of specific oligopeptides was determined by Western blotting with the MAbs. Using the same methods, amino acids were deleted one by one from the peptides of interest, enabling the two epitopes to be precisely located at amino acids 430-435 (-DRIMNP-) and 643-647 (-VFIKN-).展开更多
A Fourier-Chebyshev Petrov-Galerkin spectral method is described for high accuracy computation of linearized dynamics for flow in a circular pipe. The code used here is based on solenoidal velocity variables and is wr...A Fourier-Chebyshev Petrov-Galerkin spectral method is described for high accuracy computation of linearized dynamics for flow in a circular pipe. The code used here is based on solenoidal velocity variables and is written in FORTRAN. Systematic studies are presented of the dependence of eigenval-ues and other quantities on the axial and azimuthal wave numbers;the Reyn-olds’ number of up to 107 and the Weissenberg’s number that is considered lower here. The flow will be considered stable if all the real parts of the ei-genvalues obtained are negative and unstable if only one of these values is positive.展开更多
In this paper, a new method for solving a mathematical programming problem with linearly complementarity constraints (MPLCC) is introduced, which applies the Levenberg-Marquardt (L-M) method to solve the B-stationary ...In this paper, a new method for solving a mathematical programming problem with linearly complementarity constraints (MPLCC) is introduced, which applies the Levenberg-Marquardt (L-M) method to solve the B-stationary condition of original problem. Under the MPEC-LICQ, the proposed method is proved convergent to B-stationary point of MPLCC.展开更多
文摘Objective:To analyze the structure of aquaporins-3(AQP-3) from Schistosoma japonicum(SJAQP-3) using bioinformalical methods,and to provid of references for vaccine targets research.Methods:Protparam,BepiPred,TMHMM Server,MLRC,Geno3d,DNA star software packages were used to predict the physical and chemical properties,hydrophilicity plot, flexibility regions,antigenic index,surface probability plot,secondary structure,and tertiary structure of amino acid sequence of SJAQP-3.Results:SJAQP-3 had six transmembrane regions and two half-spanning helices that form a central channel.The half-spanning helices fold into the centre of the channel.Either of the half-spanning helix had a conserved motif of NPA common to all aquaporins.Predicted linear B-Cell epitopes were most likely at the N-terminal amino acid residues of Saa-7aa,59aa- 62aa,225aa-230aa,282aa -288aa,294aa -29Saa and 305aa -307aa area.59aa- 62aa,22Saa-230aa located outside the membrane,the others located inside the cell.Conclusions:SJAQP-3 is a integral membrane protein in Schistosoma japonicum tegument.There are six potential epitopes in SJ AQP-3.It might be a potential molecular target for the development of vaccines.
文摘目前，宝钢集团八钢公司第一座256烧结系统中，工控系统中HMI（人机界面）使用的是西门子的产品wINCC。在实际操作过程中，操作员的操作失误引起设备故障的追诉，存在着缺失记录的情况，无法在时间和过程中来分析原因和采取规避措施。因此．有必要对操作员的操作方法进行监控和记录，同时为操作员标准化操作起到推进作用。本文介绍了基于WINCC中v b script语言的强大功能来实现这一需求。
文摘目的鉴定1型登革病毒感染病人血清中1型登革病毒非结构蛋白1(nonstructural Protein 1 of Dengue virus type 1,DENV-1 NS1)B细胞线性表位。方法以重组DENV-1 NS1为抗原,免疫印迹法筛选NS1阳性病人血清。一组重叠DENV-1 NS1的重叠多肽同这些NS1阳性血清反应鉴定NS1蛋白线性B细胞表位。采用梯度稀释的1-4型重组DENVNS1竞争抑制阳性多肽同病人血清反应鉴定其反应特异性和交叉反应性。结果12份DENV-1核酸阳性病人中筛选得到5份抗DENV-1 NS1阳性病人血清。重叠多肽法、竞争抑制法和生物信息学方法筛选出3条阳性NS1多肽(氨基酸残基序列第1-15,131-145,271-285),其中aa1-15在已报道的DENV-1分离株高度保守,提示这个区域可能存在DENV-1血清型特异性表位。aa131-145和aa271-285对应的蛋白序列比对分析表明以上区域中的133-FI/LIDGP-138和271-GKLEL/M/IDF-277在已报道的4种血清型DENV分离株中高度保守,提示这2个区域存在4种血清型DENV共同表位。结论发现3个NS1上B细胞线性表位,其中aa131-145和aa271-285为首次报道,结果有助于疫苗和诊断试剂的研发。
基金The project supported by the National Natural Science Foundation of China
文摘In this paper, for general linear methods applied to strictly dissipative initial value problem in Hilbert spaces, we prove that algebraic stability implies B-convergence, which extends and improves the existing results on Runge-Kutta methods. Specializing our results for the case of multi-step Runge-Kutta methods, a series of B-convergence results are obtained.
基金supported by Graveness item of the Department of Education of Heilongjiang(10546Z004)Tackle Key Problems item of Heilongjiang(GB01B503-02+1 种基金GB04B504)Science and Technology Tackle Key Problems item of Heilongjiang during the 12th Five-year Plan Period(GA09B302)
文摘Four monoclonal antibodies (MAbs) against Goose Parvovirus (GPV) VP3 protein already available were used to precisely locate linear B-cell epitopes in VP3 of GPV. The epitopes, recognized by four MAbs, had already been identified at low levels of resolution. Complementary oligonucleotides encoding ten amino acid fragments, with five amino acid overlaps were designed with suitable sticky ends for recombination with pET-32a and subsequent expression as small-fragment fusion proteins. Antigenicity of specific oligopeptides was determined by Western blotting with the MAbs. Using the same methods, amino acids were deleted one by one from the peptides of interest, enabling the two epitopes to be precisely located at amino acids 430-435 (-DRIMNP-) and 643-647 (-VFIKN-).
文摘A Fourier-Chebyshev Petrov-Galerkin spectral method is described for high accuracy computation of linearized dynamics for flow in a circular pipe. The code used here is based on solenoidal velocity variables and is written in FORTRAN. Systematic studies are presented of the dependence of eigenval-ues and other quantities on the axial and azimuthal wave numbers;the Reyn-olds’ number of up to 107 and the Weissenberg’s number that is considered lower here. The flow will be considered stable if all the real parts of the ei-genvalues obtained are negative and unstable if only one of these values is positive.
文摘In this paper, a new method for solving a mathematical programming problem with linearly complementarity constraints (MPLCC) is introduced, which applies the Levenberg-Marquardt (L-M) method to solve the B-stationary condition of original problem. Under the MPEC-LICQ, the proposed method is proved convergent to B-stationary point of MPLCC.
基金Supported by the National Natural Science Foundation of China (31600299)the Youth Science and Technology Nova Program of Shaanxi Province,China (2022KJXX-01)the Scientific Research Program Funded by Yunnan Provincial Education Department,China (2022J0949)。
