Study on the interaction between clarithromycin and bovine serum albumin in the imitated physiology solution

The interaction between clarithromycin (CAM) and bovine serum albumin (BSA) was investigated using linear-sweep voltammetry in pH 7.4 phosphate buffer solution where CAM caused two irreversible reduction waves P2 and P3 on mercury electrode. The study showed that the formation constant and formation ratio for the interaction between CAM and BSA were 1.51 × 10(12) and 3:1 for P2, 4.53 × 10(5) and 1:1 for P3, respectively. The ion strength enhanced the hydrophobic interaction between CAM and BSA.

Dynamic characteristics of the coupled system of footbridge and human

The influence of human body on dynamic characteristics of footbridge was analyzed. A re- alistic footbridge was measured during a mass event. A simulation experiment system including a simple beam as object and a shaker as back ground excitation was built. The acceleration responses of beam were measured when person in static and active stated stood on the beam. The dynamic pa- rameters of the structure were identified by the time-domain approach and verified by theoretical and laboratory tests. The results showed that for the human-structure coupled system, nature frequency of the structure decreased and damping increased. Moreover, the increase of damping with passive person was bigger than that with active person.

Polarographic immunoassay coupled with catalysis of non-radioactive multiple iodine label

A now polarographic immunoassay was developed In this assay,human serum albumin (HSA) as the model antigen was covalently labeled with organic compound erythrosin B(EB) containing four non-radioactive iodides through Ⅰ step chemical reaction The labeling procedure is simple and the conditions needed are moderate.The molar labeling ratio of KB HSA was 12 Ⅰ The content of iodine in the conjugate obtained by the proposed procedure is ninth higher than that by the other existing methods.A heterogeneous competitive immunoassay was established by compling the catalysis of the conjugate to substrate As(Ⅲ)-Ce(Ⅳ) reaction with the linear-sweep polarographic detec-tion of As(Ⅲ) amount HSA can be determined in the HSA concentration range from 1 to 200μg/mL,with the de-tection hum of 0 66μg/ml.

Polarographic immunoassay coupled with catalytic amplification of labeled copper ions

A new approach of polarographlc immunoassay based on the catalytic amplification of the labeled metal ions and the polarographlc detection of the catalytic product was developed.In this approach,the copper ions used as the catalyst for substrate conversion instead of natural enzyme were labeled to model antigen diphtheria toxoid (DT) through the bifunctional chelating reagent diethylenetriamine pentaacetic acid (DTPA).After heterogeneous competitive imrnunoreaction,the oxidation of substrate o-phenylenediatnine (OPD) was catalyzed by the labeled copper ions to generate an electroactive product 2,3-diammophenazine (DAP); subsequently,the product DAP was detected with linear-sweep polarography The proposed assay can determine the concentration in the range of 10-1 000 ng/mL of DT,two orders of magnitude more sensitive than those based on the direct detection of the metal ion labels The proposed immunoassay can be applied to detecting various proteins of interest.