Derivative Spectroscopy and its Application at Detecting the Weak Emission/Absorption Lines

The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line.

Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines

Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery.

GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines

BACKGROUND Thrombocytopenia 2,an autosomal dominant inherited disease characterized by moderate thrombocytopenia,predisposition to myeloid malignancies and normal platelet size and function,can be caused by 5’-untranslated region(UTR)point mutations in ankyrin repeat domain containing 26(ANKRD26).Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1)have been identified as negative regulators of ANKRD26.However,the positive regulators of ANKRD26 are still unknown.AIM To prove the positive regulatory effect of GATA binding protein 2(GATA2)on ANKRD26 transcription.METHODS Human induced pluripotent stem cells derived from bone marrow(hiPSC-BM)INTRODUCTION Ankyrin repeat domain containing protein 26(ANKRD26)acts as a regulator of adipogenesis and is involved in the regulation of feeding behavior[1-3].The ANKRD26 gene is located on chromosome 10 and shares regions of homology with the primate-specific gene family POTE.According to the Human Protein Atlas database,the ANKRD26 protein is localized to the Golgi apparatus and vesicles,and its expression can be detected in nearly all human tissues[4].Moreover,UniProt annotation revealed that ANKRD26 is localized in the centrosome and contains coiled-coil domains formed by spectrin helices and ankyrin repeats[5,6].The most common disease related to ANKRD26 is thrombocytopenia 2(THC2),which is a rare autosomal dominant inherited disease characterized by lifelong mild-to-moderate thrombocytopenia and mild bleeding[7-9].Caused by the variants in the 5’-untranslated region(UTR)of ANKRD26,THC2 is defined by a decrease in the number of platelets in circulating blood and results in increased bleeding and decreased clotting ability[8,10].Due to the point mutations that occur in the 5’-UTR of ANKRD26,its negative transcription factors(TFs),Runt related transcription factor 1(RUNX1)and friend leukemia integration 1(FLI1),lose their repression effect[11].The persistent expression of ANKRD26 increases the activity of the mitogen activated protein kinase and extracellular signal regulated kinase 1/2 signaling pathways,which are potentially involved in the regulation of thrombopoietin-dependent signaling and further impair proplatelet formation by megakaryocytes(MKs)[11].However,the positive regulators of ANKRD26,which might be associated with THC2 pathology,are still unknown.

Molecular cytogenetic analyses of two new wheat-rye 6RL translocation lines with resistance to wheat powdery mildew

Rye(Secale cereale)is a valuable gene donor for wheat improvement,especially for its resistance to diseases.Developing rye-derived resistance sources is important for wheat breeding.In the present study,two wheat-rye derivatives,designated JS016 and JS110,were produced by crossing common wheat cultivar Yangmai 23 with Pakistani rye accession W2A.Using sequential genomic in situ hybridization(GISH)and multicolor fluorescence in situ hybridization(mc-FISH),JS016 and JS110 were identified as a T6BS.6RL translocation line and a T6BS.6BL6RL translocation line,respectively.Ten newly 6RL chromosome arm-specific markers were developed and used to confirm the 6RL translocation.The wheat 55K single-nucleotide polymorphism(SNP)array further verified the molecular cytogenetic identification results above and clarified their breakpoints at 430.9 and 523.0 Mb of chromosome 6B in JS016 and JS110,respectively.Resistance spectrum and allelism test demonstrated that JS016 and JS110 possessed novel powdery mildew resistance gene(s)that was derived from the 6RL translocation but differed from Pm20.Moreover,JS016 and JS110 had better agronomic traits than the previously reported 6RL translocation line carrying Pm20.To efficiently transfer and detect the 6RL translocation from JS016 and JS110,one 6RL-specific Kompetitive allele specific PCR(KASP)marker was developed and validated in high throughput marker-assisted selection(MAS).

Minimization of Electric Power Losses on 132 kV and 220 kV Uganda Electricity Transmission Lines

The classical minimization of power losses in transmission lines is dominated by artificial intelligence techniques, which do not guarantee global optimum amidst local minima. Revolutionary and evolutionary techniques are encumbered with sophisticated transformations, which weaken the techniques. Power loss minimization is crucial to the efficient design and operation of power transmission lines. Minimization of losses is one way to meet steady grid supply, especially at peak demand. Thus, this paper has presented a gradient technique to obtain optimal variables and values from the power loss model, which efficiently minimizes power losses by modifying the traditional power loss model that combines Ohm and Corona losses. Optimality tests showed that the unmodified model does not support the minimization of power losses on transmission lines as the Hessian matrix portrayed the maximization of power losses. However, the modified model is consistent with the gradient method of optimization, which yielded optimum variables and values from the power loss model developed in this study. The unmodified (modified) models for Bujagali-Kawanda 220 kV and Masaka West-Mbarara North 132 kV transmission lines in Uganda showed maximum power losses of 0.406 (0.391) and 0.452 (0.446) kW/km/phase respectively. These results indicate that the modified model is superior to the unmodified model in minimizing power losses in the transmission lines and should be implemented for the efficient design and operation of power transmission lines within and outside Uganda for the same transmission voltages.

Fertility,genome stability,and homozygosity in a diverse set of resynthesized rapeseed lines

Rapeseed(Brassica napus,AACC)was formed by hybridization between progenitor species Brassica rapa(AA)and Brassica oleracea(CC).As a result of a limited number of hybridization events between specific progenitor genotypes and strong breeding selection for oil quality traits,rapeseed has limited genetic diversity.The production of resynthesized B.napus lines via interspecific hybridization of the diploid progenitor species B.rapa and B.oleracea is one possible way to increase genetic variation in rapeseed.However,most resynthesized lines produced so far have been reported to be meiotically unstable and infertile,in contrast to established B.napus cultivars.This hinders both maintenance and use of this germplasm in breeding programs.We characterized a large set of 140 resynthesized lines produced by crosses between B.rapa and B.oleracea,as well as between B.rapa and wild C genome species(B.incana,B.hilarionis,B.montana,B.Bourgeaui,B.villosa and B.cretica)for purity(homozygosity),fertility,and genome stability.Self-pollinated seed set,seeds per ten pods as well as percentage pollen viability were used to estimate fertility.SNP genotyping was performed using the Illumina Infinium Brassica 60K array for 116 genotypes,with at least three individuals per line.Most of the material which had been advanced through multiple generations was no longer pure,with heterozygosity detected corresponding to unknown parental contributions via outcrossing.Fertility and genome stability were both genotypedependent.Most lines had high numbers of copy number variants(CNVs),indicative of meiotic instability,and high numbers of CNVs were significantly associated with reduced fertility.Eight putatively stable resynthesized B.napus lines were observed.Further investigation of these lines may reveal the mechanisms underlying this effect.Our results suggest that selection of stable resynthesized lines for breeding purposes is possible.

Multi-Equipment Detection Method for Distribution Lines Based on Improved YOLOx-s

The YOLOx-s network does not sufficiently meet the accuracy demand of equipment detection in the autonomous inspection of distribution lines by Unmanned Aerial Vehicle(UAV)due to the complex background of distribution lines,variable morphology of equipment,and large differences in equipment sizes.Therefore,aiming at the difficult detection of power equipment in UAV inspection images,we propose a multi-equipment detection method for inspection of distribution lines based on the YOLOx-s.Based on the YOLOx-s network,we make the following improvements:1)The Receptive Field Block(RFB)module is added after the shallow feature layer of the backbone network to expand the receptive field of the network.2)The Coordinate Attention(CA)module is added to obtain the spatial direction information of the targets and improve the accuracy of target localization.3)After the first fusion of features in the Path Aggregation Network(PANet),the Adaptively Spatial Feature Fusion(ASFF)module is added to achieve efficient re-fusion of multi-scale deep and shallow feature maps by assigning adaptive weight parameters to features at different scales.4)The loss function Binary Cross Entropy(BCE)Loss in YOLOx-s is replaced by Focal Loss to alleviate the difficulty of network convergence caused by the imbalance between positive and negative samples of small-sized targets.The experiments take a private dataset consisting of four types of power equipment:Transformers,Isolators,Drop Fuses,and Lightning Arrestors.On average,the mean Average Precision(mAP)of the proposed method can reach 93.64%,an increase of 3.27%.The experimental results show that the proposed method can better identify multiple types of power equipment of different scales at the same time,which helps to improve the intelligence of UAV autonomous inspection in distribution lines.

Construction of EMS-Induced Peanut Mutant Libraries and Identification of Pod-Related Traits Mutant Lines

Peanut(Arachis hypogaea L.)is an oil and economic crop of vital importance,and peanut pod is the key organ influencing the yield and processing quality.Hence,the Pod-related traits(PRTs)are considered as important agronomic traits in peanut breeding.To broaden the variability of PRTs in current peanut germplasms,three elite peanut cultivars were used to construct Ethyl methane sulfonate(EMS)-induced mutant libraries in this study.The optimal EMS treatment conditions for the three peanut varieties were determined.It was found that the median lethal dose(LD50)of EMS treatment varied greatly among different genotypes.Finally,the EMS-induced peanut mutant libraries were constructed and a total of 124 mutant lines for PRTs were identified and evaluated.Furthermore,“M-8070”,one of the mutant lines for pod constriction,was re-sequenced via high-throughput sequencing technology.The genome-wide variations between“M-8070”and its wild parent“Fuhua 8”(FH 8)were detected.2994 EMS-induced single nucleotide polymorphisms(SNPs)and 1188 insertion-deletions(InDels)between“M-8070”and its wild parent were identified.The predominant SNP mutation type was C/G to T/A transitions,while the predominant InDel mutation type was“1-bp”.We analyzed the distribution of identified mutations and annotated their functions.Most of the mutations(91.68%of the SNPs and 77.69%of the InDels)were located in the intergenic region.72 SNPs were identified in the exonic region,leading to 27 synonymous,43 nonsynonymous and 2 stop-gain variation for gene structure.13 Indels were identified in the exonic region,leading to 4 frame-shift,8 non-frame-shift and 1 stop-gain variations of genes.These mutations may lead to the phenotypic variation of“M-8070”.Our study provided valuable resources for peanut improvement and functional genomic research.

Crossing Limit Cycles of Planar Piecewise Hamiltonian Systems with Linear Centers Separated by Two Parallel Straight Lines

In this paper, we have studied several classes of planar piecewise Hamiltonian systems with three zones separated by two parallel straight lines. Firstly, we give the maximal number of limit cycles in these classes of systems with a center in two zones and without equilibrium points in the other zone (or with a center in one zone and without equilibrium points in the other zones). In addition, we also give examples to illustrate that it can reach the maximal number.

Saturated absorption spectrum of cesium micrometric-thin cell with suppressed crossover spectral lines

Micrometric-thin cells(MCs)with alkali vapor atoms have been valuable for research and applications of hyperfine Zeeman splitting and atomic magnetometers under strong magnetic fields.We theoretically and experimentally study the saturated absorption spectra using a 100-μm cesium MC,where the pump and probe beams are linearly polarized with mutually perpendicular polarizations,and the magnetic field is along the pump beam.Because of the distinctive thin chamber of the MC,crossover spectral lines in saturated absorption spectra are largely suppressed leading to clear splittings of hyperfine Zeeman transitions in experiments,and the effect of spatial magnetic field gradient is expected to be reduced.A calculation method is proposed to achieve good agreements between theoretical calculations and experimental results.This method successfully explains the suppression of crossover lines in MCs,as well as the effects of magnetic field direction,propagation and polarization directions of the pump/probe beam on saturated absorption spectrum.The saturated absorption spectrum with suppressed crossover lines is used for laser frequency stabilization,which may provide the potential value of MCs for high spatial resolution strong-field magnetometry with high sensitivity.

Data-Centric Approach to Digital Twin Modeling of Production Lines

Digital twin(DT) is a virtual replica of a physical world that has become one of the most important ideas in the manufacturing industry’s digital revolution. DT modeling is a vital issue in building a DT of a production line. In this paper, a method is proposed to address the difficulties of complicated production line business and data heterogeneity. The method focuses on essential data in the production line and creates conceptual and information models based on the ArtiFlow model and AutomationML(AML). Conceptual models are mainly used to describe and analyze the business activities of the production line, and information models describe real production lines in the form of XML files. The proposed modeling approach has been applied to a real-world clothing production line to demonstrate its feasibility and effectiveness.

A Miniaturized Broadband Wilkinson Power Divider Using Micro-Strip Branch Lines

A miniaturized broadband Wilkinson power divider is proposed. Micro-strip branch lines are introduced to replace multiple resistors used in multi-stage Wilkinson power dividers to increase the bandwidth of single-stage Wilkinson power dividers. To demonstrate its performance, an improved single-stage Wilkinson power divider with four micro-strip branch lines was designed. Simulated results show that the insert loss is better than 3.2 dB, the input return loss, output return loss, and isolation are better than 15 dB respectively, across a 76% bandwidth from 18 to 40 GHz. .

Analysis and Simulation of the Influence of Electromagnetic Fields on Living Beings near HV Power Lines Using the FDTD Method

Recent decades have seen rapid advances in the field of electrical engineering, such that our environment has become a sea of electrical and magnetic signals, raising questions about the possible effects of low-frequency electromagnetic fields on the environment and which are capable of modifying and destroying our ecosystem. Particular interest was given in this article due to a massive influx of population living near high voltage lines. The analysis and simulation of the influence of low frequency electromagnetic fields on living beings in the vicinity of high voltage sources 132 kV and 220 kV in urban areas in DR Congo is the subject of our research with a view to estimating the level of exposure of humans to low frequency electromagnetic fields. To carry out our research, we used the classic method of analyzing the field produced near a high voltage line based on Maxwell’s image theory, the Maxwell-Gauss theorem and Maxwell-Ampère theorem to model and quantify low-frequency electromagnetic fields in the vicinity of high-voltage lines. The 2D FDTD numerical formulation was developed from telegraphers’ equations and allowed us to obtain models of current and voltage induced by electromagnetic fields on living beings below and near HV lines. The different simulations carried out on the proposed models illustrate the effects of the electrical and geometric parameters of the pylons on the distribution of the electromagnetic field in the vicinity of the HV lines. The results obtained were compared to the safety limits recommended by the standards.

线卷积网络在二维线检测和三维线框重建中的应用

本文提出了一个线卷积网络,可以从图像中检测线段.通过预测每条线段的中心位置、长度和角度,该网络能够以端到端的方式检测线段.此外,根据线中心检测的特点,本文设计了一维旋转卷积模块,并通过分析,证明其满足等变性质.在实现上,本文采用改进的数据增强和非极大值抑制方法,有效的提高了线检测的性能.通过大量消融实验以及对比实验,本文提出的线卷积网络相比于当前最优算法HAWP,在精度上高出3个百分点,达到了当前最优.最后,将该方法应用于单张图三维线框重建任务,从可视化结果上看,明显优于之前的方法.

光温敏两系不育系‘稆S’的选育与应用

[目的]选育优良的光温敏两系不育系,以提高其杂种后代的产量和品质。[方法]采用光温敏两系不育系‘RGD-7S’为母本与三系保持系‘中浙B’杂交,经5年9代的系谱法选育,育成不育性稳定、稻米品质优、异交特性好、株叶形态优良的光温敏两系不育系‘稆S’,并分析了其主要特征特性及杂种优势。[结果]‘稆S’不育性稳定,育性转换临界温度<23℃,稳定不育期127 d左右。该不育系制种产量高,异交特性好,柱头外露率78.62%,其中双边外露率39.74%。采用‘稆S’配组的高产、优质两系杂交稻组合‘稆两优质美’先后通过了福建省和海南省农作物品种审定。[结论]光温敏两系不育系‘稆S’不育性稳定,育性转换临界温度低,稻米品质优,配合力强,应用前景广泛。

一种改进的概率霍夫直线检测算法

针对概率霍夫直线检测算法检测速率不高、检测精度易受其他特征和连续间断线干扰的缺点,提出了一种改进的概率霍夫直线检测算法。改进后的算法将注意力集中在直线可能存在的若干个区间,提升了检测速率与精度,在直线检测全过程监控并剔除连续间断线,提升了算法的抗干扰能力。经过100组铝锭边缘线检测的实验验证,改进后算法相较原算法平均检测速率提升至1.77倍,查准率提升至1.45倍,且改进后算法在其他图片上的检测速率和检测效果相较原算法也有明显提升。

基于直线段检测和LT描述符的矿井图像线特征匹配算法

图像匹配是同步定位与地图构建(SLAM)技术中极为重要的一环,用于根据图像之间的变换关系确定相机位姿。基于线特征的图像匹配方法具有较强的鲁棒性和抗噪能力,更加适用于井下图像匹配,基于深度学习的线描述符对线段遮挡等场景具有较高的鲁棒性,性能优于传统描述符,但卷积神经网络架构的描述符将可变长度线段抽象为固定维进行描述,不利于线段长度及视差变化较大图像的匹配。针对上述问题,提出一种基于直线段检测和线描述符的矿井图像线特征匹配算法。在频域利用单参数同态滤波降低图像的照射分量,并增强反射分量,提升亮度及对比度;在YUV空间利用对比度受限的自适应直方图均衡化(CLAHE)算法对亮度分量进行均衡,使亮度分布更加均匀;变换至RGB空间提取直线段检测(LSD)线,引入一种基于Transformer架构的LT描述符构建LSD线的特征向量,最后完成线特征匹配。实验结果表明:该算法结合了同态滤波和CLAHE算法的优点,增强后图像的亮度适中,对比度良好,灰度分布均匀,增强效果优于单参数同态滤波算法、EnlightenGAN算法;该算法提取的线特征数较原图平均提升了32.92%,在不同相似纹理占比、不同程度旋转与平移变化的井下图像匹配中鲁棒性好,平均正确匹配数为61.75对,平均精度为86.83%,优于线二进制描述符(LBD)算法、LBD_NNDR算法、LT算法,能够满足矿井图像稳健匹配的需求。

课程思政视角下校企合作课程“施工组织与管理”教学改革实践

为落实全国高校思想政治工作会议精神,响应“思政教育落实到每一节课”的号召,全面推进大思政课建设的工作;在课程思政视角下,以高职院校工程类专业群中“顶层互选”的校企合作课程“施工组织与管理”为例,从课程思政元素挖掘方法、课程思政元素渗入模式、解析思政元素融入专业课程案例等方面入手,对思政元素有效融入课程教学过程进行研究和实践,实现全员全程全方位育人,保证学生的专业知识学习与思政教育同步同向同行。

奥希替尼联合化疗在伴有EGFR突变晚期非小细胞肺癌一线治疗中的应用

1文献来源Planchard D,Jänne PA,Cheng Y,et al.Osimertinib with or without chemotherapy in EGFR⁃mutated advanced NSCLC[J].N Engl J Med,2023,389(21):1935-1948.2证据水平1b。

一种角向传输线B-dot标定平台的设计

基于感应电压叠加器感应腔角向传输线电流探头离线标定的需求,设计了离线标定平台。离线标定平台为平板传输线结构,与在线标定相比,对标定信号的畸变更低。分析了跨平台标定误差的来源,并针对性提出降低误差的措施。分析表明,安装偏心及探头纵向安装深度是跨平台标定误差的最大来源,需要在工程设计中重点关注。实际建立了离线标定平台并开展误差分析,得到跨平台标定误差3.3%的结果。