Dysregulated pseudo-hypoxia （through its effects on cell survival, angiogenesis, metabolism, invasion） and epigenetic dysregulation [through widespread suppression of tumor suppressor genes involved in cell cycle, a...Dysregulated pseudo-hypoxia （through its effects on cell survival, angiogenesis, metabolism, invasion） and epigenetic dysregulation [through widespread suppression of tumor suppressor genes involved in cell cycle, apoptosis, adhesion, immune evasion, etc. （1）] are considered to be the two central driving pathogenic features in the progression of clear cell Renal Cell Carcinoma （ccRCC） （2,3）.展开更多
With the rapid evolution of WSNs technology, it is very important to evaluate link quality quickly and accurately, so that the routing protocols can take relevant strategies in time to keep the entire network working ...With the rapid evolution of WSNs technology, it is very important to evaluate link quality quickly and accurately, so that the routing protocols can take relevant strategies in time to keep the entire network working steadily and efficiently. However, the issue of layer is still open to research. To tackle this issue, a improving link quality assessment methods on physical novel link quality assessment metric called S3LQA is proposed, which estimates the link quality of wireless sensor networks by CC2420 wireless radio frequency transceiver principles and free space propagation theory. The metric adopts both complete and incomplete packages to improve the evaluation performance effectively based on IEEE802. 15.4 frame format and DSSS-O- QPSK mechanism. The experimental results show that the proposed method can improve energy cost and achieves hatter real-timin nerformance than traditional counting-based （PRR） link aualitv assessment metric.展开更多
Background:XIAP-associated factor 1(XAF1)negatively regulates the function of the X-linked inhibitor of apoptosis protein(XIAP),a member of the IAP family that exerts antiapoptotic effects.The extracellular signal-reg...Background:XIAP-associated factor 1(XAF1)negatively regulates the function of the X-linked inhibitor of apoptosis protein(XIAP),a member of the IAP family that exerts antiapoptotic effects.The extracellular signal-regulated kinase(ERK)pathway is thought to increase cell proliferation and to protect cells from apoptosis.The aim of the study was to investigate the correlation between the ERK1/2 signaling pathway and XAF1 in colon cancer.Methods:Four human colon cancer cell lines,HCT1116 and Lovo(wildtype p53),DLD1 and SW1116(mutant p53),were used.Lovo stable transfectants with XAF1 sense and antisense were established.The effects of dominant-negative MEK1(DN-MEK1)and MEK-specific inhibitor U0126 on the ERK signaling pathway and expression of XAF1 and XIAP proteins were determined.The transcription activity of core XAF1 promoter was assessed by dual luciferase reporter assay.Cell proliferation was measured by MTT assay.Apoptosis was determined by Hoechst 33258 staining.Results:U0126 increased the expression of XAF1 in a time-and dose-dependent manner.A similar result was obtained in cells transfected with DN-MEK1 treatment.Conversely,the expression of XIAP was down-regulated.Activity of the putative promoter of the XAF1 gene was significantly increased by U0126 treatment and DN-MEK1 transient transfection.rhEGF-stimulated phosphorylation of ERK appeared to have little or no effect on XAF1 expression.Overexpression of XAF1 was more sensitive to U0126-induced apoptosis,whereas down-regulation of XAF1 by antisense reversed U0126-induced inhibition of cell proliferation.Conclusions:XAF1 expression was up-regulated by inhibition of the ERK1/2 pathway through transcriptional regulation,which required de novo protein synthesis.The results suggest that XAF1 mediates apoptosis induced by the ERK1/2 pathway in colon cancer.展开更多
Wireless Local Area Networks (WLANs) such as IEEE 802.11a/g and Hiperlan/2 utilise numerous transmission modes, each providing different throughputs and reliability levels. Many link adaptation algorithms proposed in ...Wireless Local Area Networks (WLANs) such as IEEE 802.11a/g and Hiperlan/2 utilise numerous transmission modes, each providing different throughputs and reliability levels. Many link adaptation algorithms proposed in the literature either maximise the error-free data throughput based on channel conditions or are based on the number of failed transmissions. However, these algo- rithms do not take into account the content of the data stream and strongly rely on the use of Automatic Repeat Requests (ARQs). Low latency video applications such as real-time video transmission may require no retransmission, or only a limited number of retrans- missions. Moreover, completely error-free communication is not essential, especially if robust video compression techniques are applied. In such scenarios, improved decoded video quality can be obtained with a video stream transmitted at a higher bit rate using a higher link speed but with some degree of transmission error, rather than an error-free video stream at a lower bit rate using a lower link speed. In this work, we investigate a link adaptation scheme that improves the Quality of Service (QoS) for video transmission, based on the overall received video quality (Peak Signal to Noise Ratio, PSNR), rather than by maximising the error-free throughput. We also study a practical link adaptation approach that uses PER thresholds at the PHY layer. An empirical study showed that thresholds for switching from one mode to another are much lower (almost error free) than those currently used by throughput based schemes. We show that traditional link adaptation strategies are not appropriate for real-time video transmission with no retransmis- sion. Simulation results using the H.264 video compression standard over IEEE 802.11a are presented.展开更多
文摘Dysregulated pseudo-hypoxia （through its effects on cell survival, angiogenesis, metabolism, invasion） and epigenetic dysregulation [through widespread suppression of tumor suppressor genes involved in cell cycle, apoptosis, adhesion, immune evasion, etc. （1）] are considered to be the two central driving pathogenic features in the progression of clear cell Renal Cell Carcinoma （ccRCC） （2,3）.
基金Sponsored by the National Natural Science Foundation of China(Grant No.61262020)Aeronautical Science Foundation of China(Grant No.2010ZC56008 and 2012ZC56006)Key Technology R&D Program of Jiangxi Province(Grant No.2009BGA01000 and 20111BBE50030)
文摘With the rapid evolution of WSNs technology, it is very important to evaluate link quality quickly and accurately, so that the routing protocols can take relevant strategies in time to keep the entire network working steadily and efficiently. However, the issue of layer is still open to research. To tackle this issue, a improving link quality assessment methods on physical novel link quality assessment metric called S3LQA is proposed, which estimates the link quality of wireless sensor networks by CC2420 wireless radio frequency transceiver principles and free space propagation theory. The metric adopts both complete and incomplete packages to improve the evaluation performance effectively based on IEEE802. 15.4 frame format and DSSS-O- QPSK mechanism. The experimental results show that the proposed method can improve energy cost and achieves hatter real-timin nerformance than traditional counting-based （PRR） link aualitv assessment metric.
基金Shanghai Medical Key Discipline Construction Foundation(05-Ⅲ-005-017).
文摘Background:XIAP-associated factor 1(XAF1)negatively regulates the function of the X-linked inhibitor of apoptosis protein(XIAP),a member of the IAP family that exerts antiapoptotic effects.The extracellular signal-regulated kinase(ERK)pathway is thought to increase cell proliferation and to protect cells from apoptosis.The aim of the study was to investigate the correlation between the ERK1/2 signaling pathway and XAF1 in colon cancer.Methods:Four human colon cancer cell lines,HCT1116 and Lovo(wildtype p53),DLD1 and SW1116(mutant p53),were used.Lovo stable transfectants with XAF1 sense and antisense were established.The effects of dominant-negative MEK1(DN-MEK1)and MEK-specific inhibitor U0126 on the ERK signaling pathway and expression of XAF1 and XIAP proteins were determined.The transcription activity of core XAF1 promoter was assessed by dual luciferase reporter assay.Cell proliferation was measured by MTT assay.Apoptosis was determined by Hoechst 33258 staining.Results:U0126 increased the expression of XAF1 in a time-and dose-dependent manner.A similar result was obtained in cells transfected with DN-MEK1 treatment.Conversely,the expression of XIAP was down-regulated.Activity of the putative promoter of the XAF1 gene was significantly increased by U0126 treatment and DN-MEK1 transient transfection.rhEGF-stimulated phosphorylation of ERK appeared to have little or no effect on XAF1 expression.Overexpression of XAF1 was more sensitive to U0126-induced apoptosis,whereas down-regulation of XAF1 by antisense reversed U0126-induced inhibition of cell proliferation.Conclusions:XAF1 expression was up-regulated by inhibition of the ERK1/2 pathway through transcriptional regulation,which required de novo protein synthesis.The results suggest that XAF1 mediates apoptosis induced by the ERK1/2 pathway in colon cancer.
文摘Wireless Local Area Networks (WLANs) such as IEEE 802.11a/g and Hiperlan/2 utilise numerous transmission modes, each providing different throughputs and reliability levels. Many link adaptation algorithms proposed in the literature either maximise the error-free data throughput based on channel conditions or are based on the number of failed transmissions. However, these algo- rithms do not take into account the content of the data stream and strongly rely on the use of Automatic Repeat Requests (ARQs). Low latency video applications such as real-time video transmission may require no retransmission, or only a limited number of retrans- missions. Moreover, completely error-free communication is not essential, especially if robust video compression techniques are applied. In such scenarios, improved decoded video quality can be obtained with a video stream transmitted at a higher bit rate using a higher link speed but with some degree of transmission error, rather than an error-free video stream at a lower bit rate using a lower link speed. In this work, we investigate a link adaptation scheme that improves the Quality of Service (QoS) for video transmission, based on the overall received video quality (Peak Signal to Noise Ratio, PSNR), rather than by maximising the error-free throughput. We also study a practical link adaptation approach that uses PER thresholds at the PHY layer. An empirical study showed that thresholds for switching from one mode to another are much lower (almost error free) than those currently used by throughput based schemes. We show that traditional link adaptation strategies are not appropriate for real-time video transmission with no retransmis- sion. Simulation results using the H.264 video compression standard over IEEE 802.11a are presented.
