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Corneal collagen cross-linking in patients with keratoconus from the Dresden protocol to customized solutions:theoretical basis 被引量:1
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作者 Ciro Caruso Luca D’Andrea +4 位作者 Mario Troisi Michele Rinaldi Raffaele Piscopo Salvatore Troisi Ciro Costagliola 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2024年第5期951-962,共12页
Keratoconus is an ectatic condition characterized by gradual corneal thinning,corneal protrusion,progressive irregular astigmatism,corneal fibrosis,and visual impairment.The therapeutic options regarding improvement o... Keratoconus is an ectatic condition characterized by gradual corneal thinning,corneal protrusion,progressive irregular astigmatism,corneal fibrosis,and visual impairment.The therapeutic options regarding improvement of visual function include glasses or soft contact lenses correction for initial stages,gas-permeable rigid contact lenses,scleral lenses,implantation of intrastromal corneal ring or corneal transplants for most advanced stages.In keratoconus cases showing disease progression corneal collagen crosslinking(CXL)has been proven to be an effective,minimally invasive and safe procedure.CXL consists of a photochemical reaction of corneal collagen by riboflavin stimulation with ultraviolet A radiation,resulting in stromal crosslinks formation.The aim of this review is to carry out an examination of CXL methods based on theoretical basis and mathematical models,from the original Dresden protocol to the most recent developments in the technique,reporting the changes proposed in the last 15y and examining the advantages and disadvantages of the various treatment protocols.Finally,the limits of non-standardized methods and the perspectives offered by a customization of the treatment are highlighted. 展开更多
关键词 corneal collagen cross linking KERATOCONUS custom fast protocol Dresden protocol lambert-beer law Bunsen-roscoe law
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SHEL:a semantically enhanced hardware-friendly entity linking method
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作者 亓东林 CHEN Shudong +2 位作者 DU Rong TONG Da YU Yong 《High Technology Letters》 EI CAS 2024年第1期13-22,共10页
With the help of pre-trained language models,the accuracy of the entity linking task has made great strides in recent years.However,most models with excellent performance require fine-tuning on a large amount of train... With the help of pre-trained language models,the accuracy of the entity linking task has made great strides in recent years.However,most models with excellent performance require fine-tuning on a large amount of training data using large pre-trained language models,which is a hardware threshold to accomplish this task.Some researchers have achieved competitive results with less training data through ingenious methods,such as utilizing information provided by the named entity recognition model.This paper presents a novel semantic-enhancement-based entity linking approach,named semantically enhanced hardware-friendly entity linking(SHEL),which is designed to be hardware friendly and efficient while maintaining good performance.Specifically,SHEL's semantic enhancement approach consists of three aspects:(1)semantic compression of entity descriptions using a text summarization model;(2)maximizing the capture of mention contexts using asymmetric heuristics;(3)calculating a fixed size mention representation through pooling operations.These series of semantic enhancement methods effectively improve the model's ability to capture semantic information while taking into account the hardware constraints,and significantly improve the model's convergence speed by more than 50%compared with the strong baseline model proposed in this paper.In terms of performance,SHEL is comparable to the previous method,with superior performance on six well-established datasets,even though SHEL is trained using a smaller pre-trained language model as the encoder. 展开更多
关键词 entity linking(EL) pre-trained models knowledge graph text summarization semantic enhancement
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Coherent optical frequency transfer via 972-km fiber link 被引量:1
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作者 邓雪 张翔 +8 位作者 臧琦 焦东东 王丹 刘杰 高静 许冠军 董瑞芳 刘涛 张首刚 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第2期277-282,共6页
We demonstrate coherent optical frequency dissemination over a distance of 972 km by cascading two spans where the phase noise is passively compensated for.Instead of employing a phase discriminator and a phase lockin... We demonstrate coherent optical frequency dissemination over a distance of 972 km by cascading two spans where the phase noise is passively compensated for.Instead of employing a phase discriminator and a phase locking loop in the conventional active phase control scheme,the passive phase noise cancellation is realized by feeding double-trip beat-note frequency to the driver of the acoustic optical modulator at the local site.This passive scheme exhibits fine robustness and reliability,making it suitable for long-distance and noisy fiber links.An optical regeneration station is used in the link for signal amplification and cascaded transmission.The phase noise cancellation and transfer instability of the 972-km link is investigated,and transfer instability of 1.1×10^(-19)at 10^(4)s is achieved.This work provides a promising method for realizing optical frequency distribution over thousands of kilometers by using fiber links. 展开更多
关键词 optical frequency transfer fiber link phase noise cancellation
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Time Transfer in a 1839-km Telecommunication Fiber Link Demonstrating a Picosecond-Scale Stability
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作者 郭新兴 侯丙安 +5 位作者 刘博 杨凡 孔维成 刘涛 董瑞芳 张首刚 《Chinese Physics Letters》 SCIE EI CAS CSCD 2024年第6期49-53,共5页
An implementation of high-precision time transfer over a 1839-km field fiber loop back link between two provincial capitals of China,Xi’an and Taiyuan,is reported.Time transfer stabilities of 6.5 ps at averaging time... An implementation of high-precision time transfer over a 1839-km field fiber loop back link between two provincial capitals of China,Xi’an and Taiyuan,is reported.Time transfer stabilities of 6.5 ps at averaging time of 1 s and 4.6 ps at 40000 s were achieved.The uncertainty for the time transfer system was evaluated,showing a budget of 56.2 ps.These results stand for a significant milestone in achieving high-precision time transfer over a field fiber link spanning thousands of kilometers,signifying a record-breaking achievement for the real-field time transfer in both stability and distance,which paves the way for constructing the nationwide high-precision time service via fiber network. 展开更多
关键词 FIBER NATIONWIDE link
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Robust free-space optical frequency transfer in time-varying link distances conditions
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作者 童周 刘雷 +6 位作者 王家亮 操前 金志成 应康 韩申生 韩正甫 桂有珍 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第2期271-276,共6页
Future inter-satellite clock comparison on high orbit will require optical time and frequency transmission technology between moving objects.Here,we demonstrate robust optical frequency transmission under the conditio... Future inter-satellite clock comparison on high orbit will require optical time and frequency transmission technology between moving objects.Here,we demonstrate robust optical frequency transmission under the condition of variable link distance.This variable link is accomplished by the relative motion of a single telescope fixed on the experimental platform to a corner-cube reflector(CCR)installed on a sliding guide.Two acousto–optic modulators with different frequencies are used to separate forward signal from backward signal.With active phase noise suppression,when the CCR moves back and forth at a constant velocity of 20 cm/s and an acceleration of 20 cm/s^(2),we achieve the best frequency stability of 1.9×10^(-16) at 1 s and 7.9×10^(-19) at 1000 s indoors.This work paves the way for future studying optical frequency transfer between ultra-high-orbit satellites. 展开更多
关键词 FREE-SPACE optical frequency transfer variable link distance
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Layered Coded Cache Placement and Cooperative Delivery with Sharing Links in Satellite-Terrestrial Integrated Networks
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作者 Gu Shushi Chen Zihan +2 位作者 Wu Yaonan Zhang Qinyu Wang Ye 《China Communications》 SCIE CSCD 2024年第3期217-229,共13页
Cooperative utilization of multidimensional resources including cache, power and spectrum in satellite-terrestrial integrated networks(STINs) can provide a feasible approach for massive streaming media content deliver... Cooperative utilization of multidimensional resources including cache, power and spectrum in satellite-terrestrial integrated networks(STINs) can provide a feasible approach for massive streaming media content delivery over the seamless global coverage area. However, the on-board supportable resources of a single satellite are extremely limited and lack of interaction with others. In this paper, we design a network model with two-layered cache deployment, i.e., satellite layer and ground base station layer, and two types of sharing links, i.e., terrestrial-satellite sharing(TSS) links and inter-satellite sharing(ISS) links, to enhance the capability of cooperative delivery over STINs. Thus, we use rateless codes for the content divided-packet transmission, and derive the total energy efficiency(EE) in the whole transmission procedure, which is defined as the ratio of traffic offloading and energy consumption. We formulate two optimization problems about maximizing EE in different sharing scenarios(only TSS and TSS-ISS),and propose two optimized algorithms to obtain the optimal content placement matrixes, respectively.Simulation results demonstrate that, enabling sharing links with optimized cache placement have more than 2 times improvement of EE performance than other traditional placement schemes. Particularly, TSS-ISS schemes have the higher EE performance than only TSS schemes under the conditions of enough number of satellites and smaller inter-satellite distances. 展开更多
关键词 coded content placement cooperative delivery energy efficiency sharing links STINs
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Underwater Image Classification Based on EfficientnetB0 and Two-Hidden-Layer Random Vector Functional Link
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作者 ZHOU Zhiyu LIU Mingxuan +2 位作者 JI Haodong WANG Yaming ZHU Zefei 《Journal of Ocean University of China》 CAS CSCD 2024年第2期392-404,共13页
The ocean plays an important role in maintaining the equilibrium of Earth’s ecology and providing humans access to a wealth of resources.To obtain a high-precision underwater image classification model,we propose a c... The ocean plays an important role in maintaining the equilibrium of Earth’s ecology and providing humans access to a wealth of resources.To obtain a high-precision underwater image classification model,we propose a classification model that combines an EfficientnetB0 neural network and a two-hidden-layer random vector functional link network(EfficientnetB0-TRVFL).The features of underwater images were extracted using the EfficientnetB0 neural network pretrained via ImageNet,and a new fully connected layer was trained on the underwater image dataset using the transfer learning method.Transfer learning ensures the initial performance of the network and helps in the development of a high-precision classification model.Subsequently,a TRVFL was proposed to improve the classification property of the model.Net construction of the two hidden layers exhibited a high accuracy when the same hidden layer nodes were used.The parameters of the second hidden layer were obtained using a novel calculation method,which reduced the outcome error to improve the performance instability caused by the random generation of parameters of RVFL.Finally,the TRVFL classifier was used to classify features and obtain classification results.The proposed EfficientnetB0-TRVFL classification model achieved 87.28%,74.06%,and 99.59%accuracy on the MLC2008,MLC2009,and Fish-gres datasets,respectively.The best convolutional neural networks and existing methods were stacked up through box plots and Kolmogorov-Smirnov tests,respectively.The increases imply improved systematization properties in underwater image classification tasks.The image classification model offers important performance advantages and better stability compared with existing methods. 展开更多
关键词 underwater image classification EfficientnetB0 random vector functional link convolutional neural network
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Enhanced Mechanism for Link Failure Rerouting in Software-Defined Exchange Point Networks
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作者 Abdijalil Abdullahi Selvakumar Manickam 《Computers, Materials & Continua》 SCIE EI 2024年第9期4361-4385,共25页
Internet Exchange Point(IXP)is a system that increases network bandwidth performance.Internet exchange points facilitate interconnection among network providers,including Internet Service Providers(ISPs)andContent Del... Internet Exchange Point(IXP)is a system that increases network bandwidth performance.Internet exchange points facilitate interconnection among network providers,including Internet Service Providers(ISPs)andContent Delivery Providers(CDNs).To improve service management,Internet exchange point providers have adopted the Software Defined Network(SDN)paradigm.This implementation is known as a Software-Defined Exchange Point(SDX).It improves network providers’operations and management.However,performance issues still exist,particularly with multi-hop topologies.These issues include switch memory costs,packet processing latency,and link failure recovery delays.The paper proposes Enhanced Link Failure Rerouting(ELFR),an improved mechanism for rerouting link failures in software-defined exchange point networks.The proposed mechanism aims to minimize packet processing time for fast link failure recovery and enhance path calculation efficiency while reducing switch storage overhead by exploiting the Programming Protocol-independent Packet Processors(P4)features.The paper presents the proposed mechanisms’efficiency by utilizing advanced algorithms and demonstrating improved performance in packet processing speed,path calculation effectiveness,and switch storage management compared to current mechanisms.The proposed mechanism shows significant improvements,leading to a 37.5%decrease in Recovery Time(RT)and a 33.33%decrease in both Calculation Time(CT)and Computational Overhead(CO)when compared to current mechanisms.The study highlights the effectiveness and resource efficiency of the proposed mechanism in effectively resolving crucial issues inmulti-hop software-defined exchange point networks. 展开更多
关键词 link failure recovery Internet exchange point software-defined exchange point software-defined network multihop topologies
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IO-Link SSP规范:智能传感器通信协议的关键要素分析与应用
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作者 李钟琦 周学良 季书涵 《仪器仪表标准化与计量》 2024年第3期4-6,共3页
IO-Link SSP(Smart Sensor Profile)是一种用于工业自动化领域的通信协议,旨在实现设备之间的可靠数据交换和状态监测。本文通过对IO-Link SSP规范的解析,探讨SSP在工业应用中的优势与发展。
关键词 IO-link SSP 智能传感器 规范 行规
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IO-Link技术在化工自动化控制系统中的应用 被引量:1
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作者 翟德昂 谯兴兰 《化工管理》 2024年第13期58-60,110,共4页
当前,化工自动化控制系统建设成本高、可扩展性差,数据传输类型单一,缺乏异常事件预警、远程诊断、参数设定等“智慧化工”必需的通讯功能。为解决上述问题,文章提出在化工自动化控制系统中应用IO-Link技术,介绍了IO-Link的架构、特点... 当前,化工自动化控制系统建设成本高、可扩展性差,数据传输类型单一,缺乏异常事件预警、远程诊断、参数设定等“智慧化工”必需的通讯功能。为解决上述问题,文章提出在化工自动化控制系统中应用IO-Link技术,介绍了IO-Link的架构、特点及其独特优势,在此基础上分别从化工自动化系统底层装置、GDS等化工自动化控制系统设计等方面探讨了IO-Link技术在化工自动控制系统中的应用场景及存在的问题,为“智慧化工”企业的建设提供借鉴与参考。 展开更多
关键词 智慧化工 自动化控制系统 IO-link
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Simulation Study on Dynamic Stiffness of Kick-reaction Link Actuator
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作者 HU Yixue PU Youhua LIU Peng 《International Journal of Plant Engineering and Management》 2024年第3期129-141,共13页
Under the same aerodynamic load,the load transmitted by the kick-reaction link actuator to the aircraft structure is only 1/5 to 1/3 of that of the point-to-point actuator,which can significantly reduce the weight of ... Under the same aerodynamic load,the load transmitted by the kick-reaction link actuator to the aircraft structure is only 1/5 to 1/3 of that of the point-to-point actuator,which can significantly reduce the weight of the structure,and is widely used in the main flight control surface of large and medium-sized civil airliners.In order to restrain the flutter of aircraft rudder surface,it is necessary to design the servo stiffness of the kick-reaction link actuator in the development stage,so that it can meet the dynamic stiffness requirement within the frequency range.Taking the actuator of a civil airliner as the research object,the dynamic stiffness modeling of elevator actuator is carried out on MATLAB platform,and the dynamic stiffness test is carried out to verify the correctness of dynamic stiffness modeling.The simulation and test results show that the actuator can meet the dynamic stiffness design requirements. 展开更多
关键词 kick⁃reaction link actuator dynamic stiffness MATLAB servo stiffness
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IO-Link让阀门更智能
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作者 Dominik Stephan 《流程工业》 2024年第3期20-21,共2页
迄今为止,阀门在工业生产中好像一直都扮演着默默无闻的“安全官”角色,它们控制着各种物质的流入和流出,非常耐心地等待着来自控制系统的指令,维持着生产的稳定运行。现在IO-Link作为一种数字通信接口,可以提供更多的传感器/执行器的数... 迄今为止,阀门在工业生产中好像一直都扮演着默默无闻的“安全官”角色,它们控制着各种物质的流入和流出,非常耐心地等待着来自控制系统的指令,维持着生产的稳定运行。现在IO-Link作为一种数字通信接口,可以提供更多的传感器/执行器的数据,包括过程数据、诊断数据和设备信息等,实现自主调试和双向通信,进一步优化生产线并简化整个工业生产流程。 展开更多
关键词 诊断数据 控制系统 过程数据 link 执行器 双向通信 工业生产 传感器
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倍加福发布全新带IO-Link功能F191RFID超高频读写设备
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《智慧工厂》 2024年第1期27-27,共1页
在生产过程中,为实现产品的跟踪追溯,我们需要对零部件和货物进行无缝识别及跟踪,提升整个生产制造过程的透明度,为工业4.0概念落地奠定基础。如今,倍加福新推新型F191系列RFID读写设备,它结合了先进的工业超高频技术和IO-Link通信标准... 在生产过程中,为实现产品的跟踪追溯,我们需要对零部件和货物进行无缝识别及跟踪,提升整个生产制造过程的透明度,为工业4.0概念落地奠定基础。如今,倍加福新推新型F191系列RFID读写设备,它结合了先进的工业超高频技术和IO-Link通信标准化接口,作为经济高效的使能组件,适用于内部物流、汽车制造、物料搬运等多种应用领域。 展开更多
关键词 link 生产制造过程 读写设备 物料搬运 内部物流 超高频技术 标准化接口 应用领域
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基于NC-Link的静压转台数据集控平台应用研究
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作者 刘文鑫 赵明 +3 位作者 徐妍妍 张圣平 徐黎明 程攀龙 《机械工程师》 2024年第10期130-134,140,共6页
以提升重型机床回转类静压导轨的高速性能和智能化水平为目标,设计了一套基于NC-Link协议和开源项目Thingsboard的静压转台数据集控和可视化应用框架。实现了静压油膜厚度的在线检测和自动调整,静压导轨载荷的自动识别,以及静压工作状... 以提升重型机床回转类静压导轨的高速性能和智能化水平为目标,设计了一套基于NC-Link协议和开源项目Thingsboard的静压转台数据集控和可视化应用框架。实现了静压油膜厚度的在线检测和自动调整,静压导轨载荷的自动识别,以及静压工作状态的云监控。 展开更多
关键词 NC-link 物联网平台 数据采集 云监控
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基于AMESim和Simulink联合仿真的定量泵-变量马达系统转速控制研究
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作者 邬凯 陈朋威 《价值工程》 2024年第9期136-139,共4页
本文使用了AMESim和Simulink软件构建了定量泵-变量马达系统仿真模型,用于定量泵-变量马达系统的分析,并采用Simulink为主的联合仿真方法。进行了PID和模糊PID两种控制策略的比较研究。结果表明,与传统PID控制相比,模糊PID控制策略在系... 本文使用了AMESim和Simulink软件构建了定量泵-变量马达系统仿真模型,用于定量泵-变量马达系统的分析,并采用Simulink为主的联合仿真方法。进行了PID和模糊PID两种控制策略的比较研究。结果表明,与传统PID控制相比,模糊PID控制策略在系统响应时间和稳定性方面表现更出色,尤其在恒转速控制方面表现更佳。 展开更多
关键词 定量泵-变量马达系统 联合仿真 模糊PID 恒转速控制
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Fully Distributed Learning for Deep Random Vector Functional-Link Networks
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作者 Huada Zhu Wu Ai 《Journal of Applied Mathematics and Physics》 2024年第4期1247-1262,共16页
In the contemporary era, the proliferation of information technology has led to an unprecedented surge in data generation, with this data being dispersed across a multitude of mobile devices. Facing these situations a... In the contemporary era, the proliferation of information technology has led to an unprecedented surge in data generation, with this data being dispersed across a multitude of mobile devices. Facing these situations and the training of deep learning model that needs great computing power support, the distributed algorithm that can carry out multi-party joint modeling has attracted everyone’s attention. The distributed training mode relieves the huge pressure of centralized model on computer computing power and communication. However, most distributed algorithms currently work in a master-slave mode, often including a central server for coordination, which to some extent will cause communication pressure, data leakage, privacy violations and other issues. To solve these problems, a decentralized fully distributed algorithm based on deep random weight neural network is proposed. The algorithm decomposes the original objective function into several sub-problems under consistency constraints, combines the decentralized average consensus (DAC) and alternating direction method of multipliers (ADMM), and achieves the goal of joint modeling and training through local calculation and communication of each node. Finally, we compare the proposed decentralized algorithm with several centralized deep neural networks with random weights, and experimental results demonstrate the effectiveness of the proposed algorithm. 展开更多
关键词 Distributed Optimization Deep Neural Network Random Vector Functional-link (RVFL) Network Alternating Direction Method of Multipliers (ADMM)
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多音干扰下Nakagami-m信道传输Link16数据链的性能分析 被引量:8
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作者 宁晓燕 王影 +1 位作者 孙志国 罗海玲 《系统工程与电子技术》 EI CSCD 北大核心 2023年第2期566-571,共6页
Link16数据链的应用综合广泛,由于运用了跳扩频和编码技术使其具有强大的抗干扰能力。为了探究Link16数据链在衰落信道下的响应,在加性高斯白噪信道的基础之上,研究了Link16数据链经过Nakagami-m衰落信道的系统性能。着重推导了Link16... Link16数据链的应用综合广泛,由于运用了跳扩频和编码技术使其具有强大的抗干扰能力。为了探究Link16数据链在衰落信道下的响应,在加性高斯白噪信道的基础之上,研究了Link16数据链经过Nakagami-m衰落信道的系统性能。着重推导了Link16数据链单脉冲结构在多音干扰下经过Nakagami-m衰落信道的误码率(bit error rate,BER)表达式,并给出了双脉冲结构BER公式。仿真实验结果验证了Link16数据链单脉冲结构在多音干扰下经过Nakagami-m衰落信道的BER公式的准确性,分析了多径参数对Link16数据链抗干扰性能的影响,得出增加衰落参数m和可分辨路径可以提升Link16数据链的系统性能,且当信干比一定、被干扰频点个数在25个及以上时系统BER达到10-1。 展开更多
关键词 link16数据链 NAKAGAMI-M衰落信道 多音干扰 误码率
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低信噪比场景下Link-16系统的联合频偏估计算法 被引量:1
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作者 宁晓燕 罗海玲 +1 位作者 孙志国 刁鸣 《电子与信息学报》 EI CSCD 北大核心 2023年第10期3587-3593,共7页
对于Link-16数据链终端平台在低信噪比(SNR)高动态场景下的多普勒频率偏移问题,该文设计一种新的数据结构,推导了该结构下的克拉默-拉奥下界(CRLB),并在此基础上提出一种联合频域变换与时域自相关运算的分步式频偏估计算法。其基本思想... 对于Link-16数据链终端平台在低信噪比(SNR)高动态场景下的多普勒频率偏移问题,该文设计一种新的数据结构,推导了该结构下的克拉默-拉奥下界(CRLB),并在此基础上提出一种联合频域变换与时域自相关运算的分步式频偏估计算法。其基本思想是,首先对接收信号做自相关处理,然后通过频域变换进行最大值索引,结合修正因子得到多普勒频移的粗估计值,再利用时域的改进L&R算法对接收信号进行细估计,根据两步估计算法得到最终的频偏估计值。算法应用蒙特卡罗实验仿真,仿真结果表明,与传统频偏估计算法相比,该算法的归一化均方误差更接近CRLB,在多普勒频偏为[–20 kHz,20 kHz]时,估计精度可达10^(–5)。在低信噪比环境下,所提算法能达到较为理想的估计效果,适用于Link-16数据链通信。 展开更多
关键词 link-16数据链 克拉默-拉奥下界 自相关处理 改进L&R算法 分步式估计算法
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LINK-A RNAi慢病毒载体构建及其抑制U251胶质瘤细胞增殖的研究 被引量:1
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作者 王军成 兰彦平 +3 位作者 赵岳阳 蒯涛 马东明 李敏 《宁夏医学杂志》 CAS 2023年第7期577-580,F0002,共5页
目的构建lncRNA LINK-A的特异性RNAi慢病毒载体,并借此敲减人U251胶质瘤细胞株内的LINK-A,探究LINK-A对U251胶质瘤细胞增殖的影响。方法针对人LINK-A设计siRNA序列,构建RNAi慢病毒载体质粒并进行测序鉴定;利用pHelper 1.0、pHelper 2.0... 目的构建lncRNA LINK-A的特异性RNAi慢病毒载体,并借此敲减人U251胶质瘤细胞株内的LINK-A,探究LINK-A对U251胶质瘤细胞增殖的影响。方法针对人LINK-A设计siRNA序列,构建RNAi慢病毒载体质粒并进行测序鉴定;利用pHelper 1.0、pHelper 2.0以及shRNAi 3种质粒分别转染293T细胞,筛选分离慢病毒并测定滴度;用PBS及构建好的慢病毒感染人U251胶质瘤细胞,实验分3组:空白组(PBS)、NC组(空载病毒)、LINK-A(-)组(LINK-A RNAi慢病毒),72 h后利用RT-PCR检测细胞中LINK-A的表达情况;取病毒感染成功后的U251胶质瘤细胞行平板克隆实验培养10 d,行CCK-8实验分别于24 h、48 h检测。结果RNAi慢病毒载体质粒测序结果与RNAi设计序列一致,提示LINK-A RNAi慢病毒载体构建成功,滴度为3×108 TU/mL;病毒感染U251胶质瘤细胞72h后,细胞生长状态良好,荧光持续稳定表达,LINK-A RNA相对表达量明显降低(P<0.05),表明病毒成功感染U251细胞;10 d后LINK-A(-)组细胞克隆形成数及形成率较空白组及NC组明显减少(P<0.05);与空白组及NC组相比,24 h后LINK-A(-)组OD值减少(P<0.05),72 h明显减少(P<0.05)。结论人LINK-A RNAi慢病毒构建成功;LINK-A表达降低可抑制U251胶质瘤细胞增殖。 展开更多
关键词 胶质瘤 增殖 link-A RNAI
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基于SPI-RRV指数中国气象干旱及其风险时空演变特征研究 被引量:3
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作者 杨肖丽 罗定 +4 位作者 叶周兵 谢灵枫 任立良 江善虎 袁山水 《水资源保护》 EI CSCD 北大核心 2024年第1期44-51,共8页
为全面揭示变化环境下我国多维气象干旱特征,耦合气象干旱指数(SPI)和可靠性-回弹性-脆弱性(RRV)指数,提出了一种基于SPI-RRV指数的干旱风险评价方法,定量评价了中国气象干旱及其风险的时空演变特征。结果表明:SPI-RRV指数具有特征稳定... 为全面揭示变化环境下我国多维气象干旱特征,耦合气象干旱指数(SPI)和可靠性-回弹性-脆弱性(RRV)指数,提出了一种基于SPI-RRV指数的干旱风险评价方法,定量评价了中国气象干旱及其风险的时空演变特征。结果表明:SPI-RRV指数具有特征稳定和时空可比性强的特点,能够较为准确地评估气象干旱风险时空演变特征;南方平均干旱栅格比、干旱月占比和频次大于北方,湿润区和半湿润区干旱历时短、烈度大,半干旱区和干旱区干旱历时长、烈度相对较小;干旱高风险区转移具有显著年代际变化规律,空间上从西北向西南地区转移。 展开更多
关键词 气象干旱 标准化降水指数 可靠性-回弹性-脆弱性指数 干旱风险
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