Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty acids.Stearoyl-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid bi...Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty acids.Stearoyl-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic stresses.However,the function of SAD orthologs from L.usitatissimum has not been assessed.Here,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of L.usitatissimum cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the seeds.Interestingly,ectopic expression of LuSAD2 in A.thaliana caused altered plant architecture.Similarly,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the seeds.Furthermore,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane damage.These findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.展开更多
Oilseed flax(Linum usitatissimum L.)is one of the most important oil crops in alpine regions of North and Northwest China,which is an annual herb and belongs to Linaceae family and Linum genus.According to the applica...Oilseed flax(Linum usitatissimum L.)is one of the most important oil crops in alpine regions of North and Northwest China,which is an annual herb and belongs to Linaceae family and Linum genus.According to the application,Linum is often divided into three types,production oil,extraction fiber and both for production oil and extraction fiber.Oilseed flax is native to Near East and Mediterranean coasts.It is widely distributed throughout the world,with the largest cultivated area in Asia and Europe,followed by the Americas.Canada is the largest area for planting oilseed flax and China is second.In China.展开更多
Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from ...Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from morphological characteritics, such as frond dimension, cells size and shape, their mean length/width ratios（LWR）, and cell walls constriction. Thalli of C. aerea attenuate basipetally, with diameter 270–500 μm at upper portion, 160–360 μm at middle portion, 100–160 μm at basal portion. For the upper part, the length of cells is less than their diameter. Cell walls usually constrict at the dissepiments, which are pellucid or colorless and give the filament beaded appearance. In contrast, thalli of C. linum often have a constant diameter of 90–300 μm within the same individual, cell walls usually do not constrict and cells are cylindrical or barrel shaped. The LWR is larger than that of C. aerea. Results show that the pairwise distance between two species is 3.6%–3.7% for 18 S r RNA gene and 53.5%–54.3% for ITS region. In phylogeny, they distribute at distant clades, which confirms a genetic divergence at molecular level. In addition, morphological data indicates that filament diameter of C. linum samples is highly variable, ranging from 90 μm to 300 μm. Then these two species can be considered as separate species.展开更多
Objective:The aim of this study was to evaluate the anlilungal activity of the major fraction of fatty acids methyl esters(FAMEs)isolated from Linum usitatissimum L.seeds oil collected from Bechar department(Algeria)....Objective:The aim of this study was to evaluate the anlilungal activity of the major fraction of fatty acids methyl esters(FAMEs)isolated from Linum usitatissimum L.seeds oil collected from Bechar department(Algeria).Methods:The assessment ol antifungal activity was carried out in terms of percentage of radial growth on solid medium potatoes dextrose agar PDA)and biomass growth inhibition on liquid medium(potatoes dextrose broth PDB)against two fungi.Results:The FAMEs was found to be effective in inhibiting the radial mycelial growth of Aspergillus fluctis more than Aspergillus ochraceus on all tested concentrations.The highest antifungal index was found to be(54.19%]compared to Aspergillus ochraeeus(40.48%).The results of the antifungal activity of the FAMEs inhibition of bioniass on liquid medium gave no discounted results,but this does not exclude the antifungal activity.Conclusions:We can assume that the observed antifungal potency may be due to the abundance of linoleic andα-linolenic acids in linseed oil which appears to be promising to treat fungal infections,storage fungi and food spoilage in food industry field.展开更多
Seeds of flax or linseed(Linum usitatissimum L.)are important nutraceutical foods with antioxidant,anti-inflammatory,estrogenic,laxative,and antibacterial properties.Flaxseed oil and seeds are the richest vegetarian s...Seeds of flax or linseed(Linum usitatissimum L.)are important nutraceutical foods with antioxidant,anti-inflammatory,estrogenic,laxative,and antibacterial properties.Flaxseed oil and seeds are the richest vegetarian source of omega-3 fatty acids.Consumption of flaxseeds helps in prevention and control of cardiovascular disease,neurodegenerative disorders,obesity,diabetes mellitus,polycystic ovary syndrome,gout,liver and kidney dysfunction,oxidative stress-related diseases,post-menopausal symptoms,osteoporosis,irritable bowel syndrome,dry eye disease,cystic fibrosis,diarrhea,and cancer,particularly of the mammary and prostate gland cancer.Of late,flaxseed is gaining more importance not only because of its industrial values but also due to its nutraceutical and pharmaceutical properties.The literature review was performed using PubMed,Scopus,PubMed Central,Google Scholar,and Web of Science from 1995 onwards.Data was also obtained from websites/books/book chapters.展开更多
To assess the performance and the variability of 49 flax varieties based on agronomic parameters using cluster analysis, a field experiment was conducted in 2011/2012 and 2012/2013 winter seasons at the farm of the Na...To assess the performance and the variability of 49 flax varieties based on agronomic parameters using cluster analysis, a field experiment was conducted in 2011/2012 and 2012/2013 winter seasons at the farm of the National Research Center at Nubria district, Albehira Governorate, Egypt. The results revealed high significant difference among all flax varieties in all the studied characters. Letwania-9 and Evelen cultivars surpassed all other varieties in seed and oil yields/fed. On the other hand, D variety gave the lowest value of seed yield/fed and F variety gave the lowest values of oil% and oil yield/fed. While, G Variety surpassed all other varieties in straw yield/fed. Blanka variety recorded the lowest values of straw yield/fed and biological yield/fed, while, Posna variety gave the lowest values of technical stem length (cm). In cluster analysis, all the studied characters were used to construct a distance matrix using the Euclidian coefficient, and generate dendrogram showing dissimilarity among all the varieties. Distance coefficient was ranged from 1.2 between Piltstar and Litwania-1 varieties and 10.8 between Posna and G varieties, which reveal the genetic diversity among varieties. The varieties can be divided into 4 groups based on cluster analysis.展开更多
基金supported by the National Science and Technology Innovation 2030 of China(2022ZD04010)the National Key Research and Development Program of China(2022YFD1200400)+2 种基金the Key Research and Development Program of Shaanxi Province,China(2022NY-158)the Ph D Start-up Fund of Northwest A&F University,China(Z1090121052)a grant from the Yang Ling Seed Industry Innovation Center,China(K3031122024).
文摘Flax(Linum usitatissimum L.)is a versatile crop and its seeds are a major source of unsaturated fatty acids.Stearoyl-acyl carrier protein desaturase(SAD)is a dehydrogenase enzyme that plays a key role in oleic acid biosynthesis as well as responses to biotic and abiotic stresses.However,the function of SAD orthologs from L.usitatissimum has not been assessed.Here,we found that two LuSAD genes,LuSAD1 and LuSAD2,are present in the genome of L.usitatissimum cultivar‘Longya 10’.Heterogeneous expression of either LuSAD1 or LuSAD2 in Arabidopsis thaliana resulted in higher contents of total fatty acids and oleic acid in the seeds.Interestingly,ectopic expression of LuSAD2 in A.thaliana caused altered plant architecture.Similarly,the overexpression of either LuSAD1 or LuSAD2 in Brassica napus also resulted in increased contents of total fatty acids and oleic acid in the seeds.Furthermore,we demonstrated that either LuSAD1 or LuSAD2 enhances seedling resistance to cold and drought stresses by improving antioxidant enzyme activity and nonenzymatic antioxidant levels,as well as reducing membrane damage.These findings not only broaden our knowledge of the LuSAD functions in plants,but also offer promising targets for improving the quantity and quality of oil,and the abiotic stress tolerance of oil-producing crops,through molecular manipulation.
基金financial support provided by the National Natural Science Programs of China(31360315,31760363)the China Agriculture Research System of Construct Special(CARS-14-1-16)the Fuxi Outstanding Talent Cultivation Plan of Gansu Agricultural University(Gaufx-02J05)。
文摘Oilseed flax(Linum usitatissimum L.)is one of the most important oil crops in alpine regions of North and Northwest China,which is an annual herb and belongs to Linaceae family and Linum genus.According to the application,Linum is often divided into three types,production oil,extraction fiber and both for production oil and extraction fiber.Oilseed flax is native to Near East and Mediterranean coasts.It is widely distributed throughout the world,with the largest cultivated area in Asia and Europe,followed by the Americas.Canada is the largest area for planting oilseed flax and China is second.In China.
基金The National Key Technology R&D Program of China under contract No.2012BAC07B05the National Natural Science Foundation of China under contract Nos 31400186,31270257 and 31093440+1 种基金the Science and Technology Plan Project of Guangdong Province under contract No.2012A020200007the Science and Technology Plan Project of Shantou City,China under contract No.2012–171
文摘Green macroalgae Chaetomorpha aerea and C. linum are taxonomically confused. In this paper, we tried morphological and molecular analyses to separate these two species. C. aerea and C. linum can be distinguished from morphological characteritics, such as frond dimension, cells size and shape, their mean length/width ratios（LWR）, and cell walls constriction. Thalli of C. aerea attenuate basipetally, with diameter 270–500 μm at upper portion, 160–360 μm at middle portion, 100–160 μm at basal portion. For the upper part, the length of cells is less than their diameter. Cell walls usually constrict at the dissepiments, which are pellucid or colorless and give the filament beaded appearance. In contrast, thalli of C. linum often have a constant diameter of 90–300 μm within the same individual, cell walls usually do not constrict and cells are cylindrical or barrel shaped. The LWR is larger than that of C. aerea. Results show that the pairwise distance between two species is 3.6%–3.7% for 18 S r RNA gene and 53.5%–54.3% for ITS region. In phylogeny, they distribute at distant clades, which confirms a genetic divergence at molecular level. In addition, morphological data indicates that filament diameter of C. linum samples is highly variable, ranging from 90 μm to 300 μm. Then these two species can be considered as separate species.
基金Supported from the Algerian Ministry of the higher education and scientific research,PRU project approved in 2011/2013 under the code F03820100015
文摘Objective:The aim of this study was to evaluate the anlilungal activity of the major fraction of fatty acids methyl esters(FAMEs)isolated from Linum usitatissimum L.seeds oil collected from Bechar department(Algeria).Methods:The assessment ol antifungal activity was carried out in terms of percentage of radial growth on solid medium potatoes dextrose agar PDA)and biomass growth inhibition on liquid medium(potatoes dextrose broth PDB)against two fungi.Results:The FAMEs was found to be effective in inhibiting the radial mycelial growth of Aspergillus fluctis more than Aspergillus ochraceus on all tested concentrations.The highest antifungal index was found to be(54.19%]compared to Aspergillus ochraeeus(40.48%).The results of the antifungal activity of the FAMEs inhibition of bioniass on liquid medium gave no discounted results,but this does not exclude the antifungal activity.Conclusions:We can assume that the observed antifungal potency may be due to the abundance of linoleic andα-linolenic acids in linseed oil which appears to be promising to treat fungal infections,storage fungi and food spoilage in food industry field.
文摘Seeds of flax or linseed(Linum usitatissimum L.)are important nutraceutical foods with antioxidant,anti-inflammatory,estrogenic,laxative,and antibacterial properties.Flaxseed oil and seeds are the richest vegetarian source of omega-3 fatty acids.Consumption of flaxseeds helps in prevention and control of cardiovascular disease,neurodegenerative disorders,obesity,diabetes mellitus,polycystic ovary syndrome,gout,liver and kidney dysfunction,oxidative stress-related diseases,post-menopausal symptoms,osteoporosis,irritable bowel syndrome,dry eye disease,cystic fibrosis,diarrhea,and cancer,particularly of the mammary and prostate gland cancer.Of late,flaxseed is gaining more importance not only because of its industrial values but also due to its nutraceutical and pharmaceutical properties.The literature review was performed using PubMed,Scopus,PubMed Central,Google Scholar,and Web of Science from 1995 onwards.Data was also obtained from websites/books/book chapters.
文摘To assess the performance and the variability of 49 flax varieties based on agronomic parameters using cluster analysis, a field experiment was conducted in 2011/2012 and 2012/2013 winter seasons at the farm of the National Research Center at Nubria district, Albehira Governorate, Egypt. The results revealed high significant difference among all flax varieties in all the studied characters. Letwania-9 and Evelen cultivars surpassed all other varieties in seed and oil yields/fed. On the other hand, D variety gave the lowest value of seed yield/fed and F variety gave the lowest values of oil% and oil yield/fed. While, G Variety surpassed all other varieties in straw yield/fed. Blanka variety recorded the lowest values of straw yield/fed and biological yield/fed, while, Posna variety gave the lowest values of technical stem length (cm). In cluster analysis, all the studied characters were used to construct a distance matrix using the Euclidian coefficient, and generate dendrogram showing dissimilarity among all the varieties. Distance coefficient was ranged from 1.2 between Piltstar and Litwania-1 varieties and 10.8 between Posna and G varieties, which reveal the genetic diversity among varieties. The varieties can be divided into 4 groups based on cluster analysis.
