Field pea(Pisum sativum L.) is an important protein-rich pulse crop produced globally. Increasing the lipid content of Pisum seeds through conventional and contemporary molecular breeding tools may bring added value t...Field pea(Pisum sativum L.) is an important protein-rich pulse crop produced globally. Increasing the lipid content of Pisum seeds through conventional and contemporary molecular breeding tools may bring added value to the crop. However, knowledge about genetic diversity and lipid content in field pea is limited. An understanding of genetic diversity and population structure in diverse germplasm is important and a prerequisite for genetic dissection of complex characteristics and marker-trait associations. Fifty polymorphic microsatellite markers detecting a total of 207 alleles were used to obtain information on genetic diversity, population structure and marker-trait associations. Cluster analysis was performed using UPGMA to construct a dendrogram from a pairwise similarity matrix. Pea genotypes were divided into five major clusters. A model-based population structure analysis divided the pea accessions into four groups. Percentage lipid content in 35 diverse pea accessions was used to find potential associations with the SSR markers. Markers AD73, D21, and AA5 were significantly associated with lipid content using a mixed linear model(MLM) taking population structure(Q) and relative kinship(K) into account. The results of this preliminary study suggested that the population could be used for marker-trait association mapping studies.展开更多
Background: This study was conducted to evaluate the lipid profile in nutritional categories, to characterize dyslipidemia and assess cardiovascular risk, to describe association between anthropometric markers, incide...Background: This study was conducted to evaluate the lipid profile in nutritional categories, to characterize dyslipidemia and assess cardiovascular risk, to describe association between anthropometric markers, incidence of dyslipidemia and cardiovascular risk in Cameroonian adults. Methods: A cross-sectional survey was conducted in Yaoundé from April 2014-June 2015. It included 1986 individuals, aged 20 - 65 years, both males (30.7%) and females (69.3%). Blood pressure, anthropometric measurement including weight, height, body fat, waist and hip circumference were performed. BMI was used to define nutritional status. Blood analysis included total cholesterol, HDL-cholesterol and triglycerides, insulin. Dyslipidemia was defined as hypercholesterolemia, hypertriglyceridemia, low HDL-cholesterolemia and combined dyslipidemia. Atherogenic indexes were calculated for assessment of the cardiovascular risk. Results: Out of the 1986 participants, 544 (27.4%), 616 (31%), and 826 (41.6%) were normal weight, overweight and obese respectively. In obese, lipid profile varied according to gender (p 5) was (40.50%). Low HDL-cholesterolemia (75.4%) was the main lipid abnormality found, independently of gender, age, fat location. Waist circumference (WC) unlike body mass index (BMI) and body fatness was associated to hypercholesterolemia. Conclusion: Low HDL-cholesterolemia and high atherogenic risk profile are more prevalent amongst Cameroonian adults. Promotion of healthy lifestyle including good eating habits shall be encouraged to reduce mortality from cardiovascular disease complications.展开更多
Drought is a recurrent phenomenon in many of the countries. Moisture stress during seedling stage is very critical in determining the establishment of the crop and its further development and yield. Identifying drough...Drought is a recurrent phenomenon in many of the countries. Moisture stress during seedling stage is very critical in determining the establishment of the crop and its further development and yield. Identifying drought tolerance mechanism and physiological markers of drought susceptibility is this crop during seedling stress would be useful tool in future genetic manipulation programme to establish drought tolerance in this crop. Thus the present study aimed for quickly identifying reliable physiological markers for drought susceptibility through evaluation of physiological and biochemical changes in leaves of two contrasting mungbean (Vigna radiata L. Wilczek) cultivars i.e. K 851 (drought tolerant) and PDM 84-139 (drought susceptible) during seedling development. A range of four external water potentials (i.e. -1.0, -2.0, -3.0 and -4.0 bars), besides glass distilled water as control (0.0 bar), was used. Parameters like leaf area, relative leaf water content, chlorophyll content, chlorophyll stability indices in both the cultivars were decreased with the increasing magnitude of stress. By and large phenols and ascorbic acid content were increased with the stress level but the trend was not consistent. A steady rise in proline, hydrogen peroxide content and lipid peroxidation was found with water stress. Out of two cultivars tested, drought tolerant cultivar K 851 was better in leaf water balance and higher accumulation of phenols, proline and ascorbic acid than PDM 84-139. The correlation study indicated lipid peroxidation and H<sub>2</sub>O<sub>2</sub> content as valuable physiological markers for screening of drought susceptibility.展开更多
基金supported by the Natural Sciences and Engineering Research Council of Canada Collaborative Research and Development and Lefsrud Seeds (CRDRJ385395-09)
文摘Field pea(Pisum sativum L.) is an important protein-rich pulse crop produced globally. Increasing the lipid content of Pisum seeds through conventional and contemporary molecular breeding tools may bring added value to the crop. However, knowledge about genetic diversity and lipid content in field pea is limited. An understanding of genetic diversity and population structure in diverse germplasm is important and a prerequisite for genetic dissection of complex characteristics and marker-trait associations. Fifty polymorphic microsatellite markers detecting a total of 207 alleles were used to obtain information on genetic diversity, population structure and marker-trait associations. Cluster analysis was performed using UPGMA to construct a dendrogram from a pairwise similarity matrix. Pea genotypes were divided into five major clusters. A model-based population structure analysis divided the pea accessions into four groups. Percentage lipid content in 35 diverse pea accessions was used to find potential associations with the SSR markers. Markers AD73, D21, and AA5 were significantly associated with lipid content using a mixed linear model(MLM) taking population structure(Q) and relative kinship(K) into account. The results of this preliminary study suggested that the population could be used for marker-trait association mapping studies.
文摘Background: This study was conducted to evaluate the lipid profile in nutritional categories, to characterize dyslipidemia and assess cardiovascular risk, to describe association between anthropometric markers, incidence of dyslipidemia and cardiovascular risk in Cameroonian adults. Methods: A cross-sectional survey was conducted in Yaoundé from April 2014-June 2015. It included 1986 individuals, aged 20 - 65 years, both males (30.7%) and females (69.3%). Blood pressure, anthropometric measurement including weight, height, body fat, waist and hip circumference were performed. BMI was used to define nutritional status. Blood analysis included total cholesterol, HDL-cholesterol and triglycerides, insulin. Dyslipidemia was defined as hypercholesterolemia, hypertriglyceridemia, low HDL-cholesterolemia and combined dyslipidemia. Atherogenic indexes were calculated for assessment of the cardiovascular risk. Results: Out of the 1986 participants, 544 (27.4%), 616 (31%), and 826 (41.6%) were normal weight, overweight and obese respectively. In obese, lipid profile varied according to gender (p 5) was (40.50%). Low HDL-cholesterolemia (75.4%) was the main lipid abnormality found, independently of gender, age, fat location. Waist circumference (WC) unlike body mass index (BMI) and body fatness was associated to hypercholesterolemia. Conclusion: Low HDL-cholesterolemia and high atherogenic risk profile are more prevalent amongst Cameroonian adults. Promotion of healthy lifestyle including good eating habits shall be encouraged to reduce mortality from cardiovascular disease complications.
文摘Drought is a recurrent phenomenon in many of the countries. Moisture stress during seedling stage is very critical in determining the establishment of the crop and its further development and yield. Identifying drought tolerance mechanism and physiological markers of drought susceptibility is this crop during seedling stress would be useful tool in future genetic manipulation programme to establish drought tolerance in this crop. Thus the present study aimed for quickly identifying reliable physiological markers for drought susceptibility through evaluation of physiological and biochemical changes in leaves of two contrasting mungbean (Vigna radiata L. Wilczek) cultivars i.e. K 851 (drought tolerant) and PDM 84-139 (drought susceptible) during seedling development. A range of four external water potentials (i.e. -1.0, -2.0, -3.0 and -4.0 bars), besides glass distilled water as control (0.0 bar), was used. Parameters like leaf area, relative leaf water content, chlorophyll content, chlorophyll stability indices in both the cultivars were decreased with the increasing magnitude of stress. By and large phenols and ascorbic acid content were increased with the stress level but the trend was not consistent. A steady rise in proline, hydrogen peroxide content and lipid peroxidation was found with water stress. Out of two cultivars tested, drought tolerant cultivar K 851 was better in leaf water balance and higher accumulation of phenols, proline and ascorbic acid than PDM 84-139. The correlation study indicated lipid peroxidation and H<sub>2</sub>O<sub>2</sub> content as valuable physiological markers for screening of drought susceptibility.
