Objective: To determine the effects of different strength of Murashige and Skoog(MS)media(full,1/2and1/4) in solid and liquid media on in vitro growth of Typhonium flagelliforme(T. flagelliforme), whereby an optimum m...Objective: To determine the effects of different strength of Murashige and Skoog(MS)media(full,1/2and1/4) in solid and liquid media on in vitro growth of Typhonium flagelliforme(T. flagelliforme), whereby an optimum media composition can be provided for mass propagation of T. flagelliforme.Methods: Rhizome bud of T. flagelliforme was obtained from the axenic in vitro established T. flagelliforme plantlets in Plant Tissue Culture Laboratory, Universiti Teknologi MARA, Shah Alam. Rhizome bud was used as explant and cultured onto shoot proliferation medium under different strength of MS media(full,1/2,1/4) in solid and liquid culture media.Results: After 6 weeks of culture, the number of shoot, number of leaf, number of root,height of shoot, fresh weight, dry weight and chlorophyll content of T. flagelliforme were analyzed. A comparison was made between liquid and solid culture media. The results revealed that the liquid culture media were more effective for all the growth parameters(shoot height, shoot number, leaf number, root number, fresh weight, dry weight, chlorophyll a and chlorophyll b content) compared to solid culture media. Apart from that,this study revealed the positive relationship between strength of MS media and type of culture media(solid and liquid media) to the growth of T. flagelliforme. Growth of T. flagelliforme was improved when MS strength was increased in liquid media. In contrast, growth of T. flagelliforme was improved when MS strength was decreased in solid media.Conclusions: Through this study, an optimum media composition for mass propagation of T. flagelliforme had been established by observing effects of MS media strength and type of culture media(solid and liquid media) on the growth of T. flagelliforme.展开更多
An efficient, environmentally friendly procedure was developed for the condensation of aldehydes/ketones and activated methylene compounds with basic ionic liquid as the catalyst in water. This basic ionic liquid cata...An efficient, environmentally friendly procedure was developed for the condensation of aldehydes/ketones and activated methylene compounds with basic ionic liquid as the catalyst in water. This basic ionic liquid catalyst has a very high activity for Knoevenagel condensation to give the corresponding products in 70%-97% isolated yields under mild conditions. The basic ionic liquid catalyst in aqueous system can be reused for six times without any significant loss of activity.展开更多
The work deals with a mathematical model for real-time acoustic monitoring of material parameters of media in multi-state viscoelastic engineering systems continuously operating in irregular external environments (e.g...The work deals with a mathematical model for real-time acoustic monitoring of material parameters of media in multi-state viscoelastic engineering systems continuously operating in irregular external environments (e.g., wind turbines in cold climate areas, aircrafts, etc.). This monitoring is a high-reliability time-critical task. The work consistently derives a scalar wave PDE of the Stokes type for the non-equilibrium part (NEP) of the average normal stress in a medium. The explicit expression for the NEP of the corresponding pressure and the solution-adequateness condition are also obtained. The derived Stokes-type wave equation includes the stress relaxation time and is applicable to gases, liquids, and solids.展开更多
Loop Heat Pipe (LHP) performance strongly depends on the performance of a wick that is porous media inserted in an evaporator. In this paper, the visualization results of thermo-fluid behavior on the surface of the wi...Loop Heat Pipe (LHP) performance strongly depends on the performance of a wick that is porous media inserted in an evaporator. In this paper, the visualization results of thermo-fluid behavior on the surface of the wick with microscopic infrared thermography were reported. In this study, 2 different samples that simulated a part of wick in the evaporator were used. The wicks were made by different two materials: polytetrafluoroethylene (PTFE) and stainless steel (SUS). The pore radii of PTFE wick and SUS wick are 1.2 μm and 22.5 μm. The difference of thermo-fluid behavior that was caused by the difference of material was investigated. These two materials include 4 different properties: pore radius, thermal conductivity, permeability and porosity. In order to investigate the effect of the thermal conductivity on wick’s operating mode, the phase diagram on the q-k<sub>eff</sub> plane was made. Based on the temperature line profiles, two operating modes: mode of heat conduction and mode of convection were observed. The effective thermal conductivity of the porous media has strong effect on the operating modes. In addition, the difference of heat leak through the wick that was caused by the difference of the material was discussed.展开更多
Reconstruction of supported nanocatalysts often occurs in gas-solid reactions and significantly affects the catalytic performance,yet it is much less explored in liquid-phase environment.Herein,we find that highly-dis...Reconstruction of supported nanocatalysts often occurs in gas-solid reactions and significantly affects the catalytic performance,yet it is much less explored in liquid-phase environment.Herein,we find that highly-dispersed Ag nanocatalysts,i.e.,AgOx clusters,supported on alumina,silica,and titania,can aggregate into larger Ag or Ag2O particles after immersing in liquid-phase media at room temperature.The spontaneous aggregation of AgOx clusters in liquid water is attributed to liquid-phase Ostwald ripening through dissolution of AgOx clusters into water and subsequent redeposition to form Ag2O particles.The immersion into organic solvents such as ethanol leads to reduction of AgOx clusters and further growth into Ag particles.This work reveals that liquid-phase reaction media can induce substantial structural evolution of supported nanostructured catalysts,which should be carefully considered in liquid-solid interface catalytic reactions such as electrocatalysis,environmental catalysis,and organic synthesis in liquid phase.展开更多
The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions p...The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 108 cells/ml was selected because a final spore concentration of 1.05 × 1010 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.展开更多
The transport of Cu(II)from aqueous solutions containing buffer media through hollow fiber supported liquid membrane(HFSLM)using di(2-ethylhexyl)phosphoric acid(D2EHPA)dissolved in kerosene as membrane phase and hydro...The transport of Cu(II)from aqueous solutions containing buffer media through hollow fiber supported liquid membrane(HFSLM)using di(2-ethylhexyl)phosphoric acid(D2EHPA)dissolved in kerosene as membrane phase and hydrochloric acid as striping phase was investigated.A set of factors were studied,including tube side velocity,shell side velocity,pH of the feed phase,Cu(II)concentration in the feed phase,buffer media concentration and D2EHPA concentration in the membrane phase.Experimental results indicate that the mass transfer coefficient increases with increasing both carrier concentration in the organic phase and flow rates on the tube side and shell side,and decreases with increasing initial Cu(II)concentration in the feed phase.With increasing pH value and acetate concentration in the feed phase,the mass transfer coefficient reaches a maximum value then decreases.The optimal operating conditions are obtained at pH value of 4.44 and 0.1 mol·L -1 acetic ion concentration in feed phase,and carrier volume fraction of around 10%in kerosene as organic phase.A mathematical model of the transport mechanism through HFSLM is developed.The modeled results agree well with the experimental ones.展开更多
[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation prod...[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.展开更多
基金Supported by Fundamental Research Grant Scheme with grant number(1/2014/STWN03/Ui TM/02/1)
文摘Objective: To determine the effects of different strength of Murashige and Skoog(MS)media(full,1/2and1/4) in solid and liquid media on in vitro growth of Typhonium flagelliforme(T. flagelliforme), whereby an optimum media composition can be provided for mass propagation of T. flagelliforme.Methods: Rhizome bud of T. flagelliforme was obtained from the axenic in vitro established T. flagelliforme plantlets in Plant Tissue Culture Laboratory, Universiti Teknologi MARA, Shah Alam. Rhizome bud was used as explant and cultured onto shoot proliferation medium under different strength of MS media(full,1/2,1/4) in solid and liquid culture media.Results: After 6 weeks of culture, the number of shoot, number of leaf, number of root,height of shoot, fresh weight, dry weight and chlorophyll content of T. flagelliforme were analyzed. A comparison was made between liquid and solid culture media. The results revealed that the liquid culture media were more effective for all the growth parameters(shoot height, shoot number, leaf number, root number, fresh weight, dry weight, chlorophyll a and chlorophyll b content) compared to solid culture media. Apart from that,this study revealed the positive relationship between strength of MS media and type of culture media(solid and liquid media) to the growth of T. flagelliforme. Growth of T. flagelliforme was improved when MS strength was increased in liquid media. In contrast, growth of T. flagelliforme was improved when MS strength was decreased in solid media.Conclusions: Through this study, an optimum media composition for mass propagation of T. flagelliforme had been established by observing effects of MS media strength and type of culture media(solid and liquid media) on the growth of T. flagelliforme.
基金the National Natural Science Foundation of China(No 20533010)
文摘An efficient, environmentally friendly procedure was developed for the condensation of aldehydes/ketones and activated methylene compounds with basic ionic liquid as the catalyst in water. This basic ionic liquid catalyst has a very high activity for Knoevenagel condensation to give the corresponding products in 70%-97% isolated yields under mild conditions. The basic ionic liquid catalyst in aqueous system can be reused for six times without any significant loss of activity.
文摘The work deals with a mathematical model for real-time acoustic monitoring of material parameters of media in multi-state viscoelastic engineering systems continuously operating in irregular external environments (e.g., wind turbines in cold climate areas, aircrafts, etc.). This monitoring is a high-reliability time-critical task. The work consistently derives a scalar wave PDE of the Stokes type for the non-equilibrium part (NEP) of the average normal stress in a medium. The explicit expression for the NEP of the corresponding pressure and the solution-adequateness condition are also obtained. The derived Stokes-type wave equation includes the stress relaxation time and is applicable to gases, liquids, and solids.
文摘Loop Heat Pipe (LHP) performance strongly depends on the performance of a wick that is porous media inserted in an evaporator. In this paper, the visualization results of thermo-fluid behavior on the surface of the wick with microscopic infrared thermography were reported. In this study, 2 different samples that simulated a part of wick in the evaporator were used. The wicks were made by different two materials: polytetrafluoroethylene (PTFE) and stainless steel (SUS). The pore radii of PTFE wick and SUS wick are 1.2 μm and 22.5 μm. The difference of thermo-fluid behavior that was caused by the difference of material was investigated. These two materials include 4 different properties: pore radius, thermal conductivity, permeability and porosity. In order to investigate the effect of the thermal conductivity on wick’s operating mode, the phase diagram on the q-k<sub>eff</sub> plane was made. Based on the temperature line profiles, two operating modes: mode of heat conduction and mode of convection were observed. The effective thermal conductivity of the porous media has strong effect on the operating modes. In addition, the difference of heat leak through the wick that was caused by the difference of the material was discussed.
基金National Key Research and Development Program of China(Nos.2021YFA1502800,2022YFA1504800,and 2022YFA1504500)the National Natural Science Foundation of China(Nos.21825203,22288201,22332006,and 22321002)+2 种基金Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB0600300)the Fundamental Research Funds for the Central Universities(No.20720220009)Photon Science Center for Carbon Neutrality。
文摘Reconstruction of supported nanocatalysts often occurs in gas-solid reactions and significantly affects the catalytic performance,yet it is much less explored in liquid-phase environment.Herein,we find that highly-dispersed Ag nanocatalysts,i.e.,AgOx clusters,supported on alumina,silica,and titania,can aggregate into larger Ag or Ag2O particles after immersing in liquid-phase media at room temperature.The spontaneous aggregation of AgOx clusters in liquid water is attributed to liquid-phase Ostwald ripening through dissolution of AgOx clusters into water and subsequent redeposition to form Ag2O particles.The immersion into organic solvents such as ethanol leads to reduction of AgOx clusters and further growth into Ag particles.This work reveals that liquid-phase reaction media can induce substantial structural evolution of supported nanostructured catalysts,which should be carefully considered in liquid-solid interface catalytic reactions such as electrocatalysis,environmental catalysis,and organic synthesis in liquid phase.
基金the Ministry of Agriculture and Rural Development for the financial support to this project.
文摘The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 108 cells/ml was selected because a final spore concentration of 1.05 × 1010 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.
基金Supported by the Program for New Century Excellent Talents in University (NCET-05-0122) the National Natural Science Foundation of China(20576008 20706003) the Ph.D.Programs Foundation of Ministry of Education of China(200800100001) The authors are grateful to Prof. Xiaolong Lii of Tianjin Polytechnic University for supplying PVDF hollow fibers.
文摘The transport of Cu(II)from aqueous solutions containing buffer media through hollow fiber supported liquid membrane(HFSLM)using di(2-ethylhexyl)phosphoric acid(D2EHPA)dissolved in kerosene as membrane phase and hydrochloric acid as striping phase was investigated.A set of factors were studied,including tube side velocity,shell side velocity,pH of the feed phase,Cu(II)concentration in the feed phase,buffer media concentration and D2EHPA concentration in the membrane phase.Experimental results indicate that the mass transfer coefficient increases with increasing both carrier concentration in the organic phase and flow rates on the tube side and shell side,and decreases with increasing initial Cu(II)concentration in the feed phase.With increasing pH value and acetate concentration in the feed phase,the mass transfer coefficient reaches a maximum value then decreases.The optimal operating conditions are obtained at pH value of 4.44 and 0.1 mol·L -1 acetic ion concentration in feed phase,and carrier volume fraction of around 10%in kerosene as organic phase.A mathematical model of the transport mechanism through HFSLM is developed.The modeled results agree well with the experimental ones.
基金Supported by National Natural Science Foundation of China(20776058)New Century Training Programme Foundation for the Talents by the State Education Commission (NCET-06-0646)~~
文摘[Objective] The aim was to isolate and identify a taxol-producing endophytic fungus from Taxus media. [Method] 32 strains of endophytic fungi were identified form the inner bark of T. media,and their fermentation products were detected by high performance liquid chromatography (HPLC). [Result] Through the screening,a strain of taxol-producing endophytic fungi M57 was obtained,which could produce 45-50 μg/L of taxol,and M57 was defined as Rhizopus sp. through morphological observation and 18S rDNA sequence analysis. [Conclusion] The finding of Rhizopus sp. M57 provided a promising strain for producing taxol with taxol-producing fungi fermentation process.