Based on six thermodynamic equilibria,a unified retention model of solute in liquid chromatography(LC)is first proposed.The unified model was tested and proved to be valid for a LC sys- tem with mobile phase consistin...Based on six thermodynamic equilibria,a unified retention model of solute in liquid chromatography(LC)is first proposed.The unified model was tested and proved to be valid for a LC sys- tem with mobile phase consisting of a complete range of multiple,ternary or binary components and each kind of LC except size exclusion chromatography.In addition,so long as making some assumptions and mathematical conversions,the expressions of various popular models in LC can be derived by using the unified model.展开更多
A method for rapid and simultaneous determination of multiple pyrrolidinium ionic liquid cations by ion chromatography with direct conductivity detection was developed.Chromatographic separations were performed on a c...A method for rapid and simultaneous determination of multiple pyrrolidinium ionic liquid cations by ion chromatography with direct conductivity detection was developed.Chromatographic separations were performed on a cation exchange column using ethylenediamine-acetonitrile as the mobile phase.The effects of chromatographic column and the mobile phase,as well as the column temperature on the retention of the cations were investigated.The retention rules of the cations under different chromatographic conditions were formulated.The retention of the cations followed the carbon number rule.The method has been successfully applied to the determination of three ionic liquids synthesized by a chemical laboratory.展开更多
Ionic liquids(ILs) immobilized on silica as novel high performance liquid chromatography(HPLC)stationary phases have attracted considerable attention. However, it has not been applied to protein separation. In thi...Ionic liquids(ILs) immobilized on silica as novel high performance liquid chromatography(HPLC)stationary phases have attracted considerable attention. However, it has not been applied to protein separation. In this paper, N-methylimidazolium IL-modified silica-based stationary phase(Silpr Mim)was prepared and investigated as a novel multi-interaction stationary phase charged positively for protein separation. The results indicate that all of the basic proteins tested cannot be absorbed on this novel stationary phase, whereas all of the acidic proteins tested can be retained, and the baseline separation of eight kinds of acidic protein standards can be achieved when performed in reversed phase/ion-exchange chromatography(RPLC/IEC) mode. Compared with commonly used commercial octadecylated silica(ODS) column, the novel stationary phase can show selectivity and good resolution to acidic proteins, which has a promising application in the separation and analyses of acidic proteins from the complex samples in proteomics. In addition, the chromatographic behavior of proteins, the effect of the ligand structure and the retention mechanism on this stationary phase were also investigated.展开更多
基金This work was supported by the Excellent Young Faculty Foundation of the State Education Committee of China
文摘Based on six thermodynamic equilibria,a unified retention model of solute in liquid chromatography(LC)is first proposed.The unified model was tested and proved to be valid for a LC sys- tem with mobile phase consisting of a complete range of multiple,ternary or binary components and each kind of LC except size exclusion chromatography.In addition,so long as making some assumptions and mathematical conversions,the expressions of various popular models in LC can be derived by using the unified model.
基金supported by the Natural Science Foundation of Heilongjiang Province(No.B200909)the Program for Scientific and Technological Innovation Team Construction in Universities of Heilongjiang Province(No.2011TD010)
文摘A method for rapid and simultaneous determination of multiple pyrrolidinium ionic liquid cations by ion chromatography with direct conductivity detection was developed.Chromatographic separations were performed on a cation exchange column using ethylenediamine-acetonitrile as the mobile phase.The effects of chromatographic column and the mobile phase,as well as the column temperature on the retention of the cations were investigated.The retention rules of the cations under different chromatographic conditions were formulated.The retention of the cations followed the carbon number rule.The method has been successfully applied to the determination of three ionic liquids synthesized by a chemical laboratory.
基金supported by the National 863 Program(No.2006AA02Z227)Natural Science Foundation of Shaanxi Province(No.2011JZ002)+1 种基金the Foundation of Key Laboratory in Shaanxi Province(Nos.2010JS103,11JS097,14JS098)Shaanxi Provincial Science and Technology Co-ordinating innovation projects(No.2013SZS18-K01)
文摘Ionic liquids(ILs) immobilized on silica as novel high performance liquid chromatography(HPLC)stationary phases have attracted considerable attention. However, it has not been applied to protein separation. In this paper, N-methylimidazolium IL-modified silica-based stationary phase(Silpr Mim)was prepared and investigated as a novel multi-interaction stationary phase charged positively for protein separation. The results indicate that all of the basic proteins tested cannot be absorbed on this novel stationary phase, whereas all of the acidic proteins tested can be retained, and the baseline separation of eight kinds of acidic protein standards can be achieved when performed in reversed phase/ion-exchange chromatography(RPLC/IEC) mode. Compared with commonly used commercial octadecylated silica(ODS) column, the novel stationary phase can show selectivity and good resolution to acidic proteins, which has a promising application in the separation and analyses of acidic proteins from the complex samples in proteomics. In addition, the chromatographic behavior of proteins, the effect of the ligand structure and the retention mechanism on this stationary phase were also investigated.