[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid component...[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid components in‘Xintai Tianhong’hawthorn fruit were determined by high performance liquid chromatography(HPLC).[Results]‘Xintai Tianhong’hawthorn fruit contained such four kinds of sugar components as fructose,sorbitol,glucose and sucrose,and such six kinds of organic acid components as oxalic acid,tartaric acid,malic acid,acetic acid,citric acid and succinic acid.The fruit had the highest sucrose content,accounting for 70.53%of the total sugar content,followed by fructose,glucose and sorbitol.For the organic acid components,the content of succinic acid was the highest,accounting for 47.32%of the total acid content,followed by citric acid,malic acid,oxalic acid and tartaric acid,and acetic acid had the lowest content.[Conclusions]‘Xintai Tianhong’hawthorn should be a succinic acid-type hawthorn variety,which can be used as a parent in genetic research and breeding practice.展开更多
Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the ...Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.展开更多
目的建立基于糖诱导相分离技术结合超高效液相色谱-高分辨质谱法(ultra performance liquid chromatography-high resolution mass spectrometry,UPLC-HRMS)测定牛奶中高氯酸盐含量的分析方法。方法样品制备采用乙腈为提取溶剂,木糖为...目的建立基于糖诱导相分离技术结合超高效液相色谱-高分辨质谱法(ultra performance liquid chromatography-high resolution mass spectrometry,UPLC-HRMS)测定牛奶中高氯酸盐含量的分析方法。方法样品制备采用乙腈为提取溶剂,木糖为相分离诱导剂。分析物经Poroshell 120(50 mm×2.1 mm,1.9μm)色谱柱分离,以甲醇-1%乙酸水溶液为流动相进行梯度洗脱。采用电喷雾负离子模式与单一离子监测(targeted single ion monitoring,tSIM)扫描模式,结合同位素内标法进行定量。结果在0.1~20.0μg/L的范围内,高氯酸盐线性关系良好(r^(2)=0.9988)。方法检出限(limits of detection,LODs)和定量限(limits of quantification,LOQs)分别为0.1μg/L和0.3μg/L;3个加标水平下,回收率为88.7%~116.8%,相对标准偏差为2.3%~8.1%。在30份牛奶样品的检测分析中,高氯酸盐的检出率达100%。结论该方法操作简便,对样品净化效果良好,具有低成本、高灵敏度的优点,适用于牛奶中高氯酸盐检测。展开更多
基金Supported by Agricultural Improved Variety Project of Shandong Province(2016LZGC034)
文摘[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid components in‘Xintai Tianhong’hawthorn fruit were determined by high performance liquid chromatography(HPLC).[Results]‘Xintai Tianhong’hawthorn fruit contained such four kinds of sugar components as fructose,sorbitol,glucose and sucrose,and such six kinds of organic acid components as oxalic acid,tartaric acid,malic acid,acetic acid,citric acid and succinic acid.The fruit had the highest sucrose content,accounting for 70.53%of the total sugar content,followed by fructose,glucose and sorbitol.For the organic acid components,the content of succinic acid was the highest,accounting for 47.32%of the total acid content,followed by citric acid,malic acid,oxalic acid and tartaric acid,and acetic acid had the lowest content.[Conclusions]‘Xintai Tianhong’hawthorn should be a succinic acid-type hawthorn variety,which can be used as a parent in genetic research and breeding practice.
基金Supported by the University of Malaya,Kuala Lumpur,Malaysia under SATU joint research scheme(No.ST004-2017)the International Cooperation Seeds Funding of Nanjing Agricultural University(No.2018-AH-04)
文摘Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.
文摘目的建立基于糖诱导相分离技术结合超高效液相色谱-高分辨质谱法(ultra performance liquid chromatography-high resolution mass spectrometry,UPLC-HRMS)测定牛奶中高氯酸盐含量的分析方法。方法样品制备采用乙腈为提取溶剂,木糖为相分离诱导剂。分析物经Poroshell 120(50 mm×2.1 mm,1.9μm)色谱柱分离,以甲醇-1%乙酸水溶液为流动相进行梯度洗脱。采用电喷雾负离子模式与单一离子监测(targeted single ion monitoring,tSIM)扫描模式,结合同位素内标法进行定量。结果在0.1~20.0μg/L的范围内,高氯酸盐线性关系良好(r^(2)=0.9988)。方法检出限(limits of detection,LODs)和定量限(limits of quantification,LOQs)分别为0.1μg/L和0.3μg/L;3个加标水平下,回收率为88.7%~116.8%,相对标准偏差为2.3%~8.1%。在30份牛奶样品的检测分析中,高氯酸盐的检出率达100%。结论该方法操作简便,对样品净化效果良好,具有低成本、高灵敏度的优点,适用于牛奶中高氯酸盐检测。
