The aim was to promote the cultivation and application of medicinal plants Glehnia littoralis. [Method] Glehnia littoralis was used as experi-mental materials in this study, and orthogonal design was performed to inve...The aim was to promote the cultivation and application of medicinal plants Glehnia littoralis. [Method] Glehnia littoralis was used as experi-mental materials in this study, and orthogonal design was performed to investigate the effects of light, fertilizer and irrigation on the growth of Glehnia littoralis. The biomass, leaf chlorophyl and malondialdehyde （MDA） content were determined to study its response to light, fertilizer and irrigation. [Result] The results showed that the optimum cultivation conditions of Glehnia littoralis were not completely consistent with wild living environments. Lightly shade, highly fertilization （21.38-32.08 g） and proper irrigation （500-750 ml） were suitable for artificial cultivated Glehnia littoralis. Under the condition, the biomass was enhanced, leaf chlorophyl and carotene con-tent were increased, and MDA content changed significantly. Under stress condi-tions, the biomass of Glehnia littoralis reduced, with the decrease of chlorophyl and carotenoid content. The content of chlorophyl a was more sensitive to the variation of light, fertilizer, irrigation and other environmental factors, and MDA content signifi-cantly increased by stress conditions. [Conclusion] This research can provide refer-ence for large-scale artificial cultivation of Glehnia littoralis.展开更多
The mangrove plant Heritiera littoralis D. (Sterculi-aceae) is widelydistributed in southeastern Asia and traditionally used as a fish, arrowhead, and spearhead poison .It has also been used as a folk medicine to trea...The mangrove plant Heritiera littoralis D. (Sterculi-aceae) is widelydistributed in southeastern Asia and traditionally used as a fish, arrowhead, and spearhead poison .It has also been used as a folk medicine to treat he-maturia and diarrhea for a long history inChina . Past investigations on the chemical constituents of this plant have yielded fourtriterpenes, five sesquiterpenes, a phe-nylproanoid-glycerol and four flavonoids. In the presentstudy, eight flavonoids were isolated from the 95% EtOH extract of the leaves of this plant, andidentified as quercitrin (1), quercetin (2),kaempferol-3-O-(6″-O-E-p-coumaroyl)-β-D-glucopyranoside ( 3 ), kaempferol (4), kaempferitrin (5),myricetin (6), eriodictyol (7), and catechin (8) on the basis of their spectral data.展开更多
From the underground parts of Glehnia littoralis Fr. Schmidt ex Miquel (Umbelliferae), a new compound named glehnilate was obtained. Its structure was determined by analysis of its spectral data.
From the roots ethanol extract of Glehnia littoralis, two new lignan glycosides, named glehlinoside E （1） and F （2）, were obtained. Their structures were determined to be （-）-secoisolariciresinol 4-O-β-D-（6-O-...From the roots ethanol extract of Glehnia littoralis, two new lignan glycosides, named glehlinoside E （1） and F （2）, were obtained. Their structures were determined to be （-）-secoisolariciresinol 4-O-β-D-（6-O-feruloyl） glucopyranoside （1） and （-）- secoisolariciresinol 4-O-f-β-D-（6-O-caffeloyl） glucopyranoside （2） by analysis of their spectral data, respectively.展开更多
Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in respon...Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in response to salt stress.The protein sequence,conserved domains,secondary structure,three-dimensional structure,phylogenetic relationships,and expression pattern of the GlROP6 gene were systematically analysed.Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp,which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06.Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved,and included five G-box motifs(G1–G5),an effector binding region,a Rho insert region and a C-terminal hypervariable region.According to our phylogenetic analysis,the GlROP6 protein was closely related to the ROP protein of Daucus carota subsp.Sativus.Our quantitative real-time PCR results revealed that GlROP6 was highly expressed in flower,and GlROP6 expression was significantly upregulated in G.littoralis roots treated with NaCl.This study will facilitate investigations into the function of GlROP genes in response to salt stress in G.littoralis.展开更多
More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health ca...More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health care functions.Therefore,it has a broad application prospect in medical,health care,food and other industries.In this paper,the extraction,purification,content determination and pharmacological activities of polysaccharides from Glehnia littoralis were reviewed,which provided a gauge for the further development and utilization of Glehnia littoralis.展开更多
[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the qu...[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.展开更多
Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient ...Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient enrichment enhances growth and photosynthesis of waterlogged and salinity stressed seedlings of the mangrove Heritiera littoralis Dryand. Seedlings were fertilized with a three-component granular farm NPK (Nitrogen-Phosphorous-Potassium, 20:20:20) at varied loads under waterlogging with four levels of salinity. Growth was assessed through biomass accumulation, and photosynthesis through photosynthetic quantum yield and leaf Chl content. When seedlings were exposed to both nutrient enrichment and increased salinity, a significant shift of biomass to roots was observed. Seedlings had low value of Chl a/b ratio and photosynthetic distraction resulting in mortality was high in the highest salinity and nutrient levels. These results are useful in explaining failure of regeneration in natural stands of riverine and freshwater bound mangroves following unprecedented flooding events that accrue land based nutrient loads into estuaries and deltas.展开更多
基金Supported by Jiangsu Agricultural Science and Technology Self-Innovation Project(SCX(11)2044)Jiangsu Agricultural Science and Technology Support Program(SBE201230053)~~
文摘The aim was to promote the cultivation and application of medicinal plants Glehnia littoralis. [Method] Glehnia littoralis was used as experi-mental materials in this study, and orthogonal design was performed to investigate the effects of light, fertilizer and irrigation on the growth of Glehnia littoralis. The biomass, leaf chlorophyl and malondialdehyde （MDA） content were determined to study its response to light, fertilizer and irrigation. [Result] The results showed that the optimum cultivation conditions of Glehnia littoralis were not completely consistent with wild living environments. Lightly shade, highly fertilization （21.38-32.08 g） and proper irrigation （500-750 ml） were suitable for artificial cultivated Glehnia littoralis. Under the condition, the biomass was enhanced, leaf chlorophyl and carotene con-tent were increased, and MDA content changed significantly. Under stress condi-tions, the biomass of Glehnia littoralis reduced, with the decrease of chlorophyl and carotenoid content. The content of chlorophyl a was more sensitive to the variation of light, fertilizer, irrigation and other environmental factors, and MDA content signifi-cantly increased by stress conditions. [Conclusion] This research can provide refer-ence for large-scale artificial cultivation of Glehnia littoralis.
文摘The mangrove plant Heritiera littoralis D. (Sterculi-aceae) is widelydistributed in southeastern Asia and traditionally used as a fish, arrowhead, and spearhead poison .It has also been used as a folk medicine to treat he-maturia and diarrhea for a long history inChina . Past investigations on the chemical constituents of this plant have yielded fourtriterpenes, five sesquiterpenes, a phe-nylproanoid-glycerol and four flavonoids. In the presentstudy, eight flavonoids were isolated from the 95% EtOH extract of the leaves of this plant, andidentified as quercitrin (1), quercetin (2),kaempferol-3-O-(6″-O-E-p-coumaroyl)-β-D-glucopyranoside ( 3 ), kaempferol (4), kaempferitrin (5),myricetin (6), eriodictyol (7), and catechin (8) on the basis of their spectral data.
文摘From the underground parts of Glehnia littoralis Fr. Schmidt ex Miquel (Umbelliferae), a new compound named glehnilate was obtained. Its structure was determined by analysis of its spectral data.
基金supported by the National Natural Science Foundation of China (No.30772718),
文摘From the roots ethanol extract of Glehnia littoralis, two new lignan glycosides, named glehlinoside E （1） and F （2）, were obtained. Their structures were determined to be （-）-secoisolariciresinol 4-O-β-D-（6-O-feruloyl） glucopyranoside （1） and （-）- secoisolariciresinol 4-O-f-β-D-（6-O-caffeloyl） glucopyranoside （2） by analysis of their spectral data, respectively.
基金supported by grants from the National Science Foundation of the Jiangsu Higher Education institutions of China(No.18KJB180002)the National Natural Science Foundation of China(No.31800272)。
文摘Rho-related GTPase from plants(ROP)proteins play an essential role in plant stress resistance.In this study,the full-length GlROP6 gene was cloned based on G.littoralis transcriptome sequencing data acquired in response to salt stress.The protein sequence,conserved domains,secondary structure,three-dimensional structure,phylogenetic relationships,and expression pattern of the GlROP6 gene were systematically analysed.Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp,which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06.Amino acid sequence analyses indicated that the structure of the GlROP6 protein was conserved,and included five G-box motifs(G1–G5),an effector binding region,a Rho insert region and a C-terminal hypervariable region.According to our phylogenetic analysis,the GlROP6 protein was closely related to the ROP protein of Daucus carota subsp.Sativus.Our quantitative real-time PCR results revealed that GlROP6 was highly expressed in flower,and GlROP6 expression was significantly upregulated in G.littoralis roots treated with NaCl.This study will facilitate investigations into the function of GlROP genes in response to salt stress in G.littoralis.
文摘More and more attention has been paid to the polysaccharide components of Glehnia littoralis.It has good immunoregulation,antioxidant,antitumor,hypoglycemic and other biological activities,as well as special health care functions.Therefore,it has a broad application prospect in medical,health care,food and other industries.In this paper,the extraction,purification,content determination and pharmacological activities of polysaccharides from Glehnia littoralis were reviewed,which provided a gauge for the further development and utilization of Glehnia littoralis.
基金Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GXZYKF2019-7).
文摘[Objectives]To establish a quality standard method for the determination of quercetin in leaves of Heritiera littoralis.[Methods]Quercetin was extracted from the leaves of H.littoralis by alcohol extraction,and the quercetin was quantitatively analyzed by HPLC.[Results]There was a good linear relationship between the peak area(A)and the content(M)of quercetin in the range of 0.048-0.168μg,A=2615.5M+1.5286,r=0.9999.The method was applied to the determination of ethanol extract of H.littoralis leaves,the average recovery rate was 98.77%and RSD was 1.57%.[Conclusions]The method is simple,convenient,reproducible and accurate,and provides an idea for the determination of quercetin in leaves of H.littoralis.
文摘Inundation associated with flooding and sea level rise is predicted to have major impacts on survival, growth and productivity of mangroves. A manipulative mesocosm experiment was conducted to assess whether nutrient enrichment enhances growth and photosynthesis of waterlogged and salinity stressed seedlings of the mangrove Heritiera littoralis Dryand. Seedlings were fertilized with a three-component granular farm NPK (Nitrogen-Phosphorous-Potassium, 20:20:20) at varied loads under waterlogging with four levels of salinity. Growth was assessed through biomass accumulation, and photosynthesis through photosynthetic quantum yield and leaf Chl content. When seedlings were exposed to both nutrient enrichment and increased salinity, a significant shift of biomass to roots was observed. Seedlings had low value of Chl a/b ratio and photosynthetic distraction resulting in mortality was high in the highest salinity and nutrient levels. These results are useful in explaining failure of regeneration in natural stands of riverine and freshwater bound mangroves following unprecedented flooding events that accrue land based nutrient loads into estuaries and deltas.
