The present experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide(H_2O_2) generation by mitochondria from broiler hepatocytes. Treatments were applied t...The present experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide(H_2O_2) generation by mitochondria from broiler hepatocytes. Treatments were applied to compare sources of copper(CuSO_4 versus Cu-Met) and 4 levels of dietary Cu (11,110,220 and 330 mg/kg).Day-old broilers(Cobb 500,Gallus domesticus,n=288) were randomly divided into 8 groups of 36 each and fed diets as follows:Controls(Cu 11 mg/kg) and high copper(Cu 110, 220,and 330 mg/kg),for 60 days under normal conditions.Sample collections were made at 12,36 and 60 days of age to investigate the changes in H_2O_2 generation by mitochondria from hepatocytes.Compared with those of the control diets,H_2O_2 generation by mitochondria in the high copper groups(110 to 330 mg/kg) of the two copper sources were increased(P<0.05 or P<0.01);At days 36 and 60,H_2O_2 generation by hepatic mitochondria from Cu-Met supplementation exceeded that from birds supplemented with CuSO_4 (P<0.05 or P<0.01).In addition,H_2O_2 generation by mitochondria from broilers fed with high dietary copper appeared to be associated with altered function of mitochondrial complexⅣ.The results indicated that dietary supplementation with copper induced oxidative stress damage in liver.At each level of copper supplementation,the organic Cu-Met led to more rapid H_2O_2 generation than did inorganic CuSO_4.The results also suggest that mitochondrial complexⅣmay be targeted under conditions of high dietary copper supplementation.展开更多
AIM:To investigate the progression of hepatic histopathology in serial liver biopsies from Wilson disease(WD)patients.METHODS:We report a group of 12 WD patients treated with zinc and/or penicillamine who underwent mu...AIM:To investigate the progression of hepatic histopathology in serial liver biopsies from Wilson disease(WD)patients.METHODS:We report a group of 12 WD patients treated with zinc and/or penicillamine who underwent multiple follow-up liver biopsies.Demographic,clinical and laboratory data were gathered and all patients underwent an initial biopsy and at least one repeat biopsy.RESULTS:Time to repeat biopsy ranged from 2 to 12 years.Six patients(non-progressors)showed stable hepatic histology or improvement.In one case,we observed improvement of fibrosis from stage 2 to 0.Six patients(progressors)had worsening of fibrosis.There was no significant correlation between the histological findings and serum aminotransferases or copper me-tabolism parameters.The hepatic copper concentration reached normal levels in only two patients:one from the non-progressors and one from the progressors group.The estimated rate of progression of hepatic fibrosis in the entire group was 0 units per year in the time frame between the first and the second liver biopsy(4 years),and 0.25 between the second and the third(3 years).In the progressors group,the rate of progression of liver fibrosis was estimated at 0.11 fibrosis units per year between the first and second biopsy and,0.6 fibrosis units between the second and third biopsy.CONCLUSION:The inability of clinical tools to detect fibrosis progression in WD suggests that a liver biopsy with hepatic copper quantification every 3 years should be considered.展开更多
The aim of this paper is to review the current status of liver transplantation (LT) for Wilson disease (WD), focusing on indications and controversies, especially in patients with neuropsychiatric disease, and on iden...The aim of this paper is to review the current status of liver transplantation (LT) for Wilson disease (WD), focusing on indications and controversies, especially in patients with neuropsychiatric disease, and on identification of acute liver failure (ALF) cases related to WD. LT remains the treatment of choice for patients with ALF, as initial presentation of WD or when anti-copper agents are stopped, and for patients with chronic liver disease progressed to cirrhosis, unresponsive to chelating medications or not timely treated with copper chelating agents. The indication for LT in WD remains highly debated in patients with progressive neurological deterioration and failure to improve with appropriate medical treatment. In case of Wilsonian ALF, early identification is key as mortality is 100% without emergency LT. As many of the copper metabolism parameters are believed to be less reliable in ALF, simple biochemical tests have been proposed for diagnosis of acute WD with good sensitivity and specificity. LT corrects copper metabolism and complications resulting from WD with excellent 1 and 5 year survival. Living related liver transplantation represents an alternative to deceased donor LT with excellent long-term survival, without disease recurrence. Future options mayinclude hepatocyte transplantation and gene therapy. Although both of these have shown promising results in animal models of WD, prospective human studies are much needed to demonstrate their long-term beneficial effects and their potential to replace the need for medical therapy and LT in patients with WD.展开更多
Results of research on copper nanoparticles influence on index of readiness to apoptosis and structural changes of liver, spleen, kidney tissues as well as sensomotor cerebral cortex under copper multiple introduction...Results of research on copper nanoparticles influence on index of readiness to apoptosis and structural changes of liver, spleen, kidney tissues as well as sensomotor cerebral cortex under copper multiple introductions into organism of animals are presented in the article. It is established that copper nanoparticles distribute in organs and tissues of animals and cause specific structural changes. The increase of copper nanoparticles in organism up to toxical threshold (maximum tolerated dose) results in dystrophy and tissue necrosis. Accurate expression enhancement of Caspase 3 in micro-gliocytes (brain macrophages) has been registered seven days after one-fold intramuscular introduction of copper nanoparticles (dose 2 mg/kg of animal body weight), in liver cells - three and seven days after three-fold intramuscular introduction of copper nanoparticles (total dose was 6 mg/kg of animal body weight), in proximal kidney tubules-three hours, one, three and seven days after three-fold intramuscular introduction of copper nanoparticles (with total dose 6 mg/kg of animal body weight), in spleen cells three hours, one, three and seven days after 12-fold intramuscular introduction (with total dose 24 mg/kg of animal body weight). Received data enables us to propose using index of cells readiness to apoptosis defined by Caspase 3 expression as a criterion for copper nanoparticles introduction safety assessment.展开更多
目的:研究循经穴位按摩联合李氏铜砭刮痧治疗肝郁痰凝型乳癖疼痛的临床疗效。方法:选取我院在2021年10月~2023年10月期间收治的80例肝郁痰凝型乳癖患者进行研究,根据治疗方法分为对照组39例(予以常规中西药治疗)、观察组41例(在对照组...目的:研究循经穴位按摩联合李氏铜砭刮痧治疗肝郁痰凝型乳癖疼痛的临床疗效。方法:选取我院在2021年10月~2023年10月期间收治的80例肝郁痰凝型乳癖患者进行研究,根据治疗方法分为对照组39例(予以常规中西药治疗)、观察组41例(在对照组的基础上予以循经穴位按摩联合李氏铜砭刮痧治疗)。对比两组临床疗效、疼痛程度[简式McGill疼痛询问量表(Short-form MCGill Pain Questionnair,SF-MPQ)]、乳腺超声指标及乳房肿块症状积分、氧化应激水平[丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)]、生活质量[健康调查简表(the MOS 36-item short form health survey,SF-36)]以及不良反应发生情况。结果:观察组与对照组总疗效率分别为76.92%、92.73%,观察组总疗效率高于对照组(P<0.05);两组治疗后SF-MPQ量表各指标评分均下降,且观察组比对照组低(P<0.05);治疗后两组乳腺层厚度缩小、输乳管内径缩短、症状积分减小,且观察组乳腺层厚度小于对照组,输乳管内径短于对照组,症状积分少于对照组(P<0.05);两组治疗后MDA水平下降,SOD水平上升,且观察组MDA水平低于对照组,SOD水平高于对照组(P<0.05);两组SF-36评分比治疗前均上升,且观察组比对照组高(P<0.05);观察组(14.63%)不良反应发生率高于对照组(7.69%)(P>0.05)。结论:循经穴位按摩联合李氏铜砭刮痧治疗可有效改善肝郁痰凝型乳癖患者临床疗效,缓解疼痛程度,减轻氧化应激反应程度,缩小肿块,值得应用。展开更多
基金supported by the National Natural Science Foundation of China(NSFC) awarded to Tang Zhao-xin(grant number:30671550)
文摘The present experiment was performed with the objective of examining the effects of copper sources and levels on hydrogen peroxide(H_2O_2) generation by mitochondria from broiler hepatocytes. Treatments were applied to compare sources of copper(CuSO_4 versus Cu-Met) and 4 levels of dietary Cu (11,110,220 and 330 mg/kg).Day-old broilers(Cobb 500,Gallus domesticus,n=288) were randomly divided into 8 groups of 36 each and fed diets as follows:Controls(Cu 11 mg/kg) and high copper(Cu 110, 220,and 330 mg/kg),for 60 days under normal conditions.Sample collections were made at 12,36 and 60 days of age to investigate the changes in H_2O_2 generation by mitochondria from hepatocytes.Compared with those of the control diets,H_2O_2 generation by mitochondria in the high copper groups(110 to 330 mg/kg) of the two copper sources were increased(P<0.05 or P<0.01);At days 36 and 60,H_2O_2 generation by hepatic mitochondria from Cu-Met supplementation exceeded that from birds supplemented with CuSO_4 (P<0.05 or P<0.01).In addition,H_2O_2 generation by mitochondria from broilers fed with high dietary copper appeared to be associated with altered function of mitochondrial complexⅣ.The results indicated that dietary supplementation with copper induced oxidative stress damage in liver.At each level of copper supplementation,the organic Cu-Met led to more rapid H_2O_2 generation than did inorganic CuSO_4.The results also suggest that mitochondrial complexⅣmay be targeted under conditions of high dietary copper supplementation.
文摘AIM:To investigate the progression of hepatic histopathology in serial liver biopsies from Wilson disease(WD)patients.METHODS:We report a group of 12 WD patients treated with zinc and/or penicillamine who underwent multiple follow-up liver biopsies.Demographic,clinical and laboratory data were gathered and all patients underwent an initial biopsy and at least one repeat biopsy.RESULTS:Time to repeat biopsy ranged from 2 to 12 years.Six patients(non-progressors)showed stable hepatic histology or improvement.In one case,we observed improvement of fibrosis from stage 2 to 0.Six patients(progressors)had worsening of fibrosis.There was no significant correlation between the histological findings and serum aminotransferases or copper me-tabolism parameters.The hepatic copper concentration reached normal levels in only two patients:one from the non-progressors and one from the progressors group.The estimated rate of progression of hepatic fibrosis in the entire group was 0 units per year in the time frame between the first and the second liver biopsy(4 years),and 0.25 between the second and the third(3 years).In the progressors group,the rate of progression of liver fibrosis was estimated at 0.11 fibrosis units per year between the first and second biopsy and,0.6 fibrosis units between the second and third biopsy.CONCLUSION:The inability of clinical tools to detect fibrosis progression in WD suggests that a liver biopsy with hepatic copper quantification every 3 years should be considered.
文摘The aim of this paper is to review the current status of liver transplantation (LT) for Wilson disease (WD), focusing on indications and controversies, especially in patients with neuropsychiatric disease, and on identification of acute liver failure (ALF) cases related to WD. LT remains the treatment of choice for patients with ALF, as initial presentation of WD or when anti-copper agents are stopped, and for patients with chronic liver disease progressed to cirrhosis, unresponsive to chelating medications or not timely treated with copper chelating agents. The indication for LT in WD remains highly debated in patients with progressive neurological deterioration and failure to improve with appropriate medical treatment. In case of Wilsonian ALF, early identification is key as mortality is 100% without emergency LT. As many of the copper metabolism parameters are believed to be less reliable in ALF, simple biochemical tests have been proposed for diagnosis of acute WD with good sensitivity and specificity. LT corrects copper metabolism and complications resulting from WD with excellent 1 and 5 year survival. Living related liver transplantation represents an alternative to deceased donor LT with excellent long-term survival, without disease recurrence. Future options mayinclude hepatocyte transplantation and gene therapy. Although both of these have shown promising results in animal models of WD, prospective human studies are much needed to demonstrate their long-term beneficial effects and their potential to replace the need for medical therapy and LT in patients with WD.
文摘Results of research on copper nanoparticles influence on index of readiness to apoptosis and structural changes of liver, spleen, kidney tissues as well as sensomotor cerebral cortex under copper multiple introductions into organism of animals are presented in the article. It is established that copper nanoparticles distribute in organs and tissues of animals and cause specific structural changes. The increase of copper nanoparticles in organism up to toxical threshold (maximum tolerated dose) results in dystrophy and tissue necrosis. Accurate expression enhancement of Caspase 3 in micro-gliocytes (brain macrophages) has been registered seven days after one-fold intramuscular introduction of copper nanoparticles (dose 2 mg/kg of animal body weight), in liver cells - three and seven days after three-fold intramuscular introduction of copper nanoparticles (total dose was 6 mg/kg of animal body weight), in proximal kidney tubules-three hours, one, three and seven days after three-fold intramuscular introduction of copper nanoparticles (with total dose 6 mg/kg of animal body weight), in spleen cells three hours, one, three and seven days after 12-fold intramuscular introduction (with total dose 24 mg/kg of animal body weight). Received data enables us to propose using index of cells readiness to apoptosis defined by Caspase 3 expression as a criterion for copper nanoparticles introduction safety assessment.
文摘目的:研究循经穴位按摩联合李氏铜砭刮痧治疗肝郁痰凝型乳癖疼痛的临床疗效。方法:选取我院在2021年10月~2023年10月期间收治的80例肝郁痰凝型乳癖患者进行研究,根据治疗方法分为对照组39例(予以常规中西药治疗)、观察组41例(在对照组的基础上予以循经穴位按摩联合李氏铜砭刮痧治疗)。对比两组临床疗效、疼痛程度[简式McGill疼痛询问量表(Short-form MCGill Pain Questionnair,SF-MPQ)]、乳腺超声指标及乳房肿块症状积分、氧化应激水平[丙二醛(Malondialdehyde,MDA)、超氧化物歧化酶(Superoxide Dismutase,SOD)]、生活质量[健康调查简表(the MOS 36-item short form health survey,SF-36)]以及不良反应发生情况。结果:观察组与对照组总疗效率分别为76.92%、92.73%,观察组总疗效率高于对照组(P<0.05);两组治疗后SF-MPQ量表各指标评分均下降,且观察组比对照组低(P<0.05);治疗后两组乳腺层厚度缩小、输乳管内径缩短、症状积分减小,且观察组乳腺层厚度小于对照组,输乳管内径短于对照组,症状积分少于对照组(P<0.05);两组治疗后MDA水平下降,SOD水平上升,且观察组MDA水平低于对照组,SOD水平高于对照组(P<0.05);两组SF-36评分比治疗前均上升,且观察组比对照组高(P<0.05);观察组(14.63%)不良反应发生率高于对照组(7.69%)(P>0.05)。结论:循经穴位按摩联合李氏铜砭刮痧治疗可有效改善肝郁痰凝型乳癖患者临床疗效,缓解疼痛程度,减轻氧化应激反应程度,缩小肿块,值得应用。
