lnc-ob1通过促进白色脂肪棕色化改善肥胖小鼠胰岛素抵抗

【目的】旨在检测骨生成相关长链非编码核糖核酸(long non-coding ribonucleic acids osteoblastogenensis associated 1,lnc-ob1)在不同脂肪组织中的差异表达规律以及对白色脂肪棕色化的调控作用。【方法】将18只雄性C57BL/6J小鼠随机分为对照组、高脂饲喂组和高脂饲喂加lnc-ob1过表达处理组3个试验组,进行的高脂高糖饮食诱导4周。检测小鼠体重、脂肪组织重量变化,以及血糖、甘油三酯、胆固醇和低密度脂肪酸含量变化。利用HE染色观察小鼠肝脏和脂肪组织的形态变化,通过逆转录-荧光定量聚合酶链式反应(quantitative reverse transcription polymerase chain reaction,RT-qPCR)和Western blot检测成脂、脂肪产热以及脂肪棕色化相关基因和蛋白的表达量。【结果】与对照组相比,高脂饲喂小鼠(P<0.05)肝脏组织脂质沉积明显减轻。RT-qPCR结果显示lnc-ob1过表达组显著促进了脂质代谢基因、脂肪产热以及脂肪棕色化基因的表达,Western blot结果进一步证实lnc-ob1过表达促进UCP1产热蛋白的表达。【结论】lnc-ob1通过UCP1产热基因促进白色脂肪棕色化,改善了肥胖小鼠的胰岛素抵抗,降低了异位脂肪的过度沉积缓解了高脂高糖饮食诱导的小鼠肥胖症。

The long noncoding RNA lnc-ob1 facilitates bone formation by upregulating Osterix in osteoblasts

With the support by the National Natural Science Foundation of China,the research team directed by Prof.Wang XiaoGang(王晓刚)at the Beijing Advanced Innovation Center for Big Data-Based Precision Medicine,Beihang University,recently reported that lncRNA regulates osteoblast activity and bone formation in mice by up-regulating the osteogenic transcription factor Osterix,which was published in Nature Metabolism(2019,1:485—496).