LncR NA H19 and Target Gene-mediated Cleft Palate Induced by TCDD

This study investigated the role of long non‐coding RNAs（lnc RNAs） in the development of the palatal tissues. Cleft palates in mice were induced by 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin（TCDD）. Expression levels of long non‐coding RNA H19（lncR NA H19） and insulin‐like growth factor 2（IGF2） gene were measured by quantitative real‐time polymerase chain reaction（q RT‐PCR）. The rate of occurrence of cleft palate was found to be 100% by TCDD exposure, and TCDD could cause short upper limb, cerebral fissure, webbed neck, and short neck. The expression levels of lnc RNA H19 and IGF2 gene specifically showed embryo age‐related differences on E13, E14, and E15 in the palatal tissues. The expression levels of lnc RNA H19 and IGF2 gene showed an inverse relationship on E13, E14, and E15. These findings demonstrated that lnc RNA H19 and IGF2 can mediate the development of mouse cleft palate.

LncRNA在宫颈癌组织中的表达谱变化及意义

目的研究宫颈癌组织中lncRNA的表达变化情况,为探讨lncRNA参与宫颈癌发生与发展提供分子依据。方法取20例宫颈癌患者的宫颈癌组织和20例正常宫颈组织,应用高通量lncRNA芯片技术分别检测两组中lncRNA表达谱的变化情况,并应用qRT-PCR技术对结果加以验证。结果与正常宫颈组织相比,宫颈癌组织中共有30586条lncRNA和13982条mRNA存在差异性表达,通过GO聚类分析表明,上调mRNA和下调mRNA分别参与多种不同的生物过程。通过进一步分析发现变化差异性最明显的10个lncRNA可能通过影响相应的mRNA来发挥其生物学功能。qRT-PCR结果与芯片检测结果一致,提示4个lncRNA(BC008359,RP11-521B24.5,BRE-AS1,和RP11-229P13.15)在宫颈癌组织中发挥重要作用。结论本实验阐述了宫颈癌组织中lncRNA的变化情况,为探讨lncRNA参与宫颈癌发生与发展以及治疗提供新的分子依据。

垂体腺瘤发病的分子机制研究进展

垂体腺瘤是发生在腺垂体的良性肿瘤，是颅内常见的肿瘤之一，通过放射学检查和尸检，垂体腺瘤的发病率高达22．5％左右。除泌乳素型外，手术治疗是垂体腺瘤患者的首选治疗方法。由于垂体腺瘤的发生机制尚不完全清楚，垂体腺瘤的诊断、治疗和预后尚不尽如人意。