Posterior capsular opacification(PCO)is linked to the pathological process of lens epithelial cells,which includes proliferation,migration,and epithelial-mesenchymal transition(EMT).Our goal was to investigate whether...Posterior capsular opacification(PCO)is linked to the pathological process of lens epithelial cells,which includes proliferation,migration,and epithelial-mesenchymal transition(EMT).Our goal was to investigate whether long noncoding RNA(lncRNA)XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells.EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h,and PCO microenvironment was simulated.The expressions levels of lncRNA XIST,miR-124,and Slug were measured by realtime polymerase chain reaction(RT-PCR)and western blot.The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by using cell transfection,cell counting kit-8(CCK-8),immunofluorescence staining,transwell assay,wound-healing assay,RT-PCR,western blot and dual-luciferase reporter assay.The expression of Slug and lncRNA XIST was markedly increased,while miR-124 was downregulated in TGF-β2-treated SRA01/04 cells compared with the control group.Knockdown of lncRNA XIST reduced EMT,migration,and cell viability in TGF-β2-induced SRA01/04 cells;moreover,it adversely modulated miR-124 and adjusted the expression of Slug in SRA01/04 cells,while these effects were diminished by co-transfection with AMOmiR-124.Our data demonstrated that lncRNA XIST functioned as a competitive endogenous RNA(ceRNA)of miR-124 to modulate the expression level of Slug,thereby modulating EMT,migration,and cell viability in SRA01/04 cells.In conclusion,lncRNA XIST has a crucial role in promoting TGF-β2-induced EMT via modulating the miR-124/Slug axis in SRA01/04 cells,and it may provide a novel therapeutic option for PCO treatment.展开更多
Perfluorooctane sulfonate （PFOS） is a class of stable organic compounds with wide industrial,commercial, and consumer applications, such as in textiles, paper, pesticides, and shampoos;. It is readily absorbed, but ...Perfluorooctane sulfonate （PFOS） is a class of stable organic compounds with wide industrial,commercial, and consumer applications, such as in textiles, paper, pesticides, and shampoos;. It is readily absorbed, but poorly eliminated, with the elimination half-life of approximately 5 years;.Hence, there have been concerns regarding its potential damage to human health. Some studies展开更多
基金supported by the Joint Foundation for Regional Innovation and Development(U20A20363)the Natural Science Foundation of Heilongjiang Province(LH2020H039)the National Natural Science Foundation of China(No.81970776).
文摘Posterior capsular opacification(PCO)is linked to the pathological process of lens epithelial cells,which includes proliferation,migration,and epithelial-mesenchymal transition(EMT).Our goal was to investigate whether long noncoding RNA(lncRNA)XIST contributes to EMT via targeting miR-124/Slug axis in TGF-β2-induced SRA01/04 cells.EMT was induced by stimulating SRA01/04 cells with 10 ng/mL TGF-β2 for 24 h,and PCO microenvironment was simulated.The expressions levels of lncRNA XIST,miR-124,and Slug were measured by realtime polymerase chain reaction(RT-PCR)and western blot.The role and mechanism of lncRNA XIST in promoting EMT of TGF-β2-treated SRA01/04 cells were investigated by using cell transfection,cell counting kit-8(CCK-8),immunofluorescence staining,transwell assay,wound-healing assay,RT-PCR,western blot and dual-luciferase reporter assay.The expression of Slug and lncRNA XIST was markedly increased,while miR-124 was downregulated in TGF-β2-treated SRA01/04 cells compared with the control group.Knockdown of lncRNA XIST reduced EMT,migration,and cell viability in TGF-β2-induced SRA01/04 cells;moreover,it adversely modulated miR-124 and adjusted the expression of Slug in SRA01/04 cells,while these effects were diminished by co-transfection with AMOmiR-124.Our data demonstrated that lncRNA XIST functioned as a competitive endogenous RNA(ceRNA)of miR-124 to modulate the expression level of Slug,thereby modulating EMT,migration,and cell viability in SRA01/04 cells.In conclusion,lncRNA XIST has a crucial role in promoting TGF-β2-induced EMT via modulating the miR-124/Slug axis in SRA01/04 cells,and it may provide a novel therapeutic option for PCO treatment.
基金supported by awards from National Natural Science Foundation of China [No.81703260]the Education Department of Jiangsu Province [No.16KJB330010]+2 种基金the Science and Technology Department of Jiangsu Province [No.BK20160227]the China Postdoctoral Science Foundation funded project [No.2016M601892]the Priority Academic Program for the Development of Jiangsu Higher Education Institutions(PAPD)
文摘Perfluorooctane sulfonate （PFOS） is a class of stable organic compounds with wide industrial,commercial, and consumer applications, such as in textiles, paper, pesticides, and shampoos;. It is readily absorbed, but poorly eliminated, with the elimination half-life of approximately 5 years;.Hence, there have been concerns regarding its potential damage to human health. Some studies
