Avoiding experimental bias by systematic antibody validation

Antibodies are valued reagents in most modern laboratories and indispensable for many experiments.They can be utilized in a wide range of experimental setups designed to elucidate various scientific questions.In our laboratory,antibodies are used to investigate protein localization,quantity and phosphorylation state,

Improved allometric equations for tree aboveground biomass estimation in tropical dipterocarp forests of Kalimantan,Indonesia

Background： Currently, the common and feasible way to estimate the most accurate forest biomass requires ground measurements and allometric models.Previous studies have been conducted on allometric equations development for estimating tree aboveground biomass（AGB） of tropical dipterocarp forests（TDFs） in Kalimantan（Indonesian Borneo）.However, before the use of existing equations, a validation for the selection of the best allometric equation is required to assess the model bias and precision.This study aims at evaluating the validity of local and pantropical equations; developing new allometric equations for estimating tree AGB in TDFs of Kalimantan; and validating the new equations using independent datasets.Methods： We used 108 tree samples from destructive sampling to develop the allometric equations, with maximum tree diameter of 175 cm and another 109 samples from previous studies for validating our equations.We performed ordinary least squares linear regression to explore the relationship between the AGB and the predictor variables in the natural logarithmic form.Results： This study found that most of the existing local equations tended to be biased and imprecise, with mean relative error and mean absolute relative error more than 0.1 and 0.3, respectively.We developed new allometric equations for tree AGB estimation in the TDFs of Kalimantan.Through a validation using an independent dataset,we found that our equations were reliable in estimating tree AGB in TDF.The pantropical equation, which includes tree diameter, wood density and total height as predictor variables performed only slightly worse than our new models.Conclusions： Our equations improve the precision and reduce the bias of AGB estimates of TDFs.Local models developed from small samples tend to systematically bias.A validation of existing AGB models is essential before the use of the models.

Two Kinds Equal Frequency Circuits to Achieve Locally Resonant Band Gap of a Circular Plate Attached Alternately by Piezoelectric Unimorphs

A circular thin plate is proposed for vibration attenuation,which is attached alternately by annular piezoelectric unimorphs with resonant shunt circuits.Two kinds of equal frequency resonant shunt circuits are designed to achieve an integrated locally resonant（LR）band gap（BG） with a much smaller transmission factor：（1） the structure is arrayed periodically while the resonant shunt circuits are aperiodic;（2） the resonant shunt circuits are periodic while the structure is aperiodic.The transmission factor curve is calculated,which is validated by the finite element method.Dependences of the LR BG performance upon the geometric and electric parameters are also analyzed.