<strong>Object</strong>: To analyze porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 infection cases via the N protein gene and its encoded amino acid sequence and to provide a t...<strong>Object</strong>: To analyze porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 infection cases via the N protein gene and its encoded amino acid sequence and to provide a theoretical basis for the epidemiological study, prevention and control of porcine reproductive and respiratory syndrome (PRRS). <strong>Methods</strong>: In clinically suspected PRRSV infections, viruses were isolated by extracting viral nucleic acid and amplifying the N protein gene by RT-PCR. Then, the product was purified and sequenced to acquire the whole gene sequence of the N protein and its encoded amino acid sequence. DNASTAR software was used to analyze the homology, the genetic evolution and the derivation of the variability of amino acids of the N protein gene from 13 PRRSV strains and classical domestic and foreign strains. <strong>Results</strong>: Among the thirteen strains of PRRSV isolated from this study, ten strains had the greatest homology with the JXA1 strain (98.9% - 100%), and they belonged to the sublineage 8.7. The remaining three strains had the greatest homology with the NADC30 strain (95.4% - 97.1%), and they belonged to lineage one. The analysis of the variability of N protein amino acids showed that there were high frequency mutations in the five loci of 13 isolated strains of PRRSV as follows: 15th amino acid (10/13), 46<sup>th</sup> amino acid (11/13), 91st amino acid (10/13), 109th amino acid (10/13), and 117th amino acid (10/13). <strong>Conclusion</strong>: In recent years, sublineage 8.7 was the dominant pedigree in field PRRSV epidemic strains in China with lineage one occupying a certain proportion of the field. Four high frequency mutations existed in N protein antigen epitopes of isolated strains from the region. The nuclear localization signal (NLS) structure, specifically the 46<sup>th</sup> amino acid residue of the N protein, was mutated and genetically stable.展开更多
To overcome the drawback of a short operation range and low-resolution of a passive location system using a civil communication signal, the new idea that utilizes code division multiple access （CDMA） signal and repe...To overcome the drawback of a short operation range and low-resolution of a passive location system using a civil communication signal, the new idea that utilizes code division multiple access （CDMA） signal and repeater is disposed off. First, the CDMA passive location model and observation function are given, and the error source and error range are analyzed. Subsequently, the CDMA passive location algorithm in a repeater environment is described and simulated. The simulation result shows that the algorithm can provide the location value with high accuracy.展开更多
文摘<strong>Object</strong>: To analyze porcine reproductive and respiratory syndrome virus (PRRSV) strains from 13 infection cases via the N protein gene and its encoded amino acid sequence and to provide a theoretical basis for the epidemiological study, prevention and control of porcine reproductive and respiratory syndrome (PRRS). <strong>Methods</strong>: In clinically suspected PRRSV infections, viruses were isolated by extracting viral nucleic acid and amplifying the N protein gene by RT-PCR. Then, the product was purified and sequenced to acquire the whole gene sequence of the N protein and its encoded amino acid sequence. DNASTAR software was used to analyze the homology, the genetic evolution and the derivation of the variability of amino acids of the N protein gene from 13 PRRSV strains and classical domestic and foreign strains. <strong>Results</strong>: Among the thirteen strains of PRRSV isolated from this study, ten strains had the greatest homology with the JXA1 strain (98.9% - 100%), and they belonged to the sublineage 8.7. The remaining three strains had the greatest homology with the NADC30 strain (95.4% - 97.1%), and they belonged to lineage one. The analysis of the variability of N protein amino acids showed that there were high frequency mutations in the five loci of 13 isolated strains of PRRSV as follows: 15th amino acid (10/13), 46<sup>th</sup> amino acid (11/13), 91st amino acid (10/13), 109th amino acid (10/13), and 117th amino acid (10/13). <strong>Conclusion</strong>: In recent years, sublineage 8.7 was the dominant pedigree in field PRRSV epidemic strains in China with lineage one occupying a certain proportion of the field. Four high frequency mutations existed in N protein antigen epitopes of isolated strains from the region. The nuclear localization signal (NLS) structure, specifically the 46<sup>th</sup> amino acid residue of the N protein, was mutated and genetically stable.
基金the Key Project of Ministry of Education (207097)Education Natural Science Foundation Project of CQCSTC (2006BB2376)
文摘To overcome the drawback of a short operation range and low-resolution of a passive location system using a civil communication signal, the new idea that utilizes code division multiple access （CDMA） signal and repeater is disposed off. First, the CDMA passive location model and observation function are given, and the error source and error range are analyzed. Subsequently, the CDMA passive location algorithm in a repeater environment is described and simulated. The simulation result shows that the algorithm can provide the location value with high accuracy.
