Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich pla...Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.展开更多
Recent developments in synthetic bone grafting materials and adjuvant therapeutic agents have opened the door to the regenerative reconstruction of critical-size long bone segmental defects resulting from trauma,osteo...Recent developments in synthetic bone grafting materials and adjuvant therapeutic agents have opened the door to the regenerative reconstruction of critical-size long bone segmental defects resulting from trauma,osteoporotic fractures or tumour resections.Polymeric scaffolds with controlled macroporosities,degradability,useful surgical handling characteristics,and the ability to deliver biotherapeutics to promote new bone ingrowth have been developed for this challenging orthopaedic application.This review highlights major classes of degradable synthetic polymers and their biomineral composites,including conventional and amphiphilic polyesters,polyanhydrides,polycarbonates,and polyethylene glycol-based hydrogels,that have been explored for the regenerative reconstruction of critical-size long bone segmental defects over the past two decades.The pros and cons of these synthetic scaffold materials are presented in the context of enabling or impeding the functional(mechanical and radiographic)repair of a long bone segmental defect,with the long bone regeneration outcomes compared with healthy long bone controls or results achieved with current grafting standards.展开更多
文摘Background：The treatment for long bone defects has been a hot topic in the field of regenerative medicine.This study aimed to evaluate the therapeutic effects of calcium sulfate （CS） combined with platelet-rich plasma （PRP） on long bone defect restoration.Methods：A radial bone defect model was constructed through an osteotomy using New Zealand rabbits.The rabbits were randomly divided into four groups （n =10 in each group）：a CS combined with PRP （CS-PRP） group,a CS group,a PRP group,and a positive （recombinant human bone morphogenetic protein-2） control group.PRP was prepared from autologous blood using a two-step centrifugation process.CS-PRP was obtained by mixing hemihydrate CS with PRP.Radiographs and histologic micrographs were generated.The percentage of bone regenerated bone area in each rabbit was calculated at 10 weeks.One-way analysis of variance was performed in this study.Results：The radiographs and histologic micrographs showed bone restoration in the CS-PRP and positive control groups,while nonunion was observed in the CS and PRP groups.The percentages of bone regenerated bone area in the CS-PRP （84.60 ± 2.87%） and positive control （52.21 ± 4.53%） groups were significantly greater than those in the CS group （12.34 ± 2.17%） and PRP group （16.52 ± 4.22%） （P 〈 0.001）.In addition,the bone strength of CS-PRP group （43.l 0 ± 4.10%） was significantly greater than that of the CS group （20.10 ± 3.70%） or PRP group （25.10 ± 2.10%） （P 〈 0.001）.Conclusion：CS-PRP functions as an effective treatment for long bone defects through stimulating bone regeneration and enhancing new bone strength.
基金This work is supported by an Alex Lemonade Stand Foundation Innovation Grant and a BRIDGE Award from the University of Massachusetts Medical School.
文摘Recent developments in synthetic bone grafting materials and adjuvant therapeutic agents have opened the door to the regenerative reconstruction of critical-size long bone segmental defects resulting from trauma,osteoporotic fractures or tumour resections.Polymeric scaffolds with controlled macroporosities,degradability,useful surgical handling characteristics,and the ability to deliver biotherapeutics to promote new bone ingrowth have been developed for this challenging orthopaedic application.This review highlights major classes of degradable synthetic polymers and their biomineral composites,including conventional and amphiphilic polyesters,polyanhydrides,polycarbonates,and polyethylene glycol-based hydrogels,that have been explored for the regenerative reconstruction of critical-size long bone segmental defects over the past two decades.The pros and cons of these synthetic scaffold materials are presented in the context of enabling or impeding the functional(mechanical and radiographic)repair of a long bone segmental defect,with the long bone regeneration outcomes compared with healthy long bone controls or results achieved with current grafting standards.
