BACKGROUND Hepatocellular carcinoma(HCC)is a common malignant gastrointestinal tumor.There are currently few clinical diagnostic and prognostic markers for HCC.LncRNA cancer susceptibility candidate 9(CASC9)is a long-...BACKGROUND Hepatocellular carcinoma(HCC)is a common malignant gastrointestinal tumor.There are currently few clinical diagnostic and prognostic markers for HCC.LncRNA cancer susceptibility candidate 9(CASC9)is a long-chain non-coding RNA discovered in recent years,and previous studies have found that lncRNA CASC9 participates in the occurrence and development of HCC,but its clinical value remains unclear.AIM To determine the expression of lncRNA CASC9 in HCC and its diagnostic and prognostic value.METHODS Data on CASC9 expression in patients with HCC were collected from the Cancer Genome Atlas(TCGA)database to analyze the relationship between CASC9 and patient survival.A total of 80 HCC patients treated in The First Affiliated Hospital of Guangxi Medical University from May 2012 to January 2014 were enrolled in the patient group,and 50 healthy subjects were enrolled in the control group during the same period.CASC9 expression in the two groups was determined using quantitative real-time polymerase chain reaction,and its diagnostic and prognostic value was analyzed based on the CASC9 data and pathological data in these HCC patients.The relationship between CASC9 and patient survival was assessed during the 5-year follow-up period.RESULTS Analysis of data from TCGA database revealed that control samples showed significantly lower CASC9 expression than carcinoma tissue samples(P<0.001);the low CASC9 expression group had a higher survival rate than the high CASC9 expression group(P=0.011),and the patient group showed significantly increased expression of serum CASC9,with the area under the curve(AUC)of 0.933.CASC9 expression was related to tumor size,combined hepatitis,tumor,node,metastasis(TNM)staging,lymph node metastasis,differentiation and alpha fetoprotein,and the high CASC9 expression group showed lower 1-year,3-year and 5-year survival rates than the low CASC9 expression group(all aP<0.05).Multivariate Cox regression analysis revealed that TNM staging,lymph node metastasis,differentiation,alpha fetoprotein and CASC9 were independent factors affecting the prognosis of patients.Stage I+II patients with lymph node metastasis,low differentiation,and alpha fetoprotein>200 ng/mL had a poor 5-year survival rate.CONCLUSION High CASC9 expression is beneficial in the prognosis of HCC patients.CASC9 is expected to be a potential diagnostic and prognostic indicator of HCC.展开更多
目的 检测长链非编码RNA(LncRNA)癌易感性候选基因7(CASC7)、微小RNA-21(miR-21)在支气管哮喘急性发作期患儿外周血中的表达水平并分析其临床意义。方法 选取2020年6月至2021年10月在襄阳市中心医院就诊的支气管哮喘急性发作期患儿115例...目的 检测长链非编码RNA(LncRNA)癌易感性候选基因7(CASC7)、微小RNA-21(miR-21)在支气管哮喘急性发作期患儿外周血中的表达水平并分析其临床意义。方法 选取2020年6月至2021年10月在襄阳市中心医院就诊的支气管哮喘急性发作期患儿115例(急性发作期组)以及支气管哮喘缓解期患儿97例(缓解期组),根据病情程度将急性发作期组患儿分为轻度组45例,中度组39例,重度组31例。另收集同期体检健康儿童100例(健康人对照组)。采用实时荧光定量PCR法(RT-qPCR)检测各组外周血LncRNA CASC7、miR-21的表达水平;检测第1秒用力呼气容积(forced expiratory volume in 1 second, FEV1)、用力肺活量(forced vital capacity, FVC)、最大呼吸峰流速(peak expiratory flow, PEF)等肺功能指标;采用免疫比浊法检测超敏C反应蛋白(hs-CRP)的表达,ELISA法检测TNF-α、IL-6的表达水平;Pearson法分析LncRNA CASC7、miR-21的表达与hs-CRP、TNF-α、IL-6及FEV1、FVC、PEF的相关性,并进一步分析LncRNA CASC7与miR-21的相关性;ROC曲线分析LncRNA CASC7、miR-21对支气管哮喘急性发作期患儿的鉴别诊断价值。结果 RT-qPCR检测结果显示,健康人对照组、急性发作期组、缓解期组患儿外周血LncRNA CASC7的表达水平(1.09±0.18 vs 0.93±0.12 vs 0.65±0.10)依次降低(F=289.447,P<0.05),miR-21的表达水平(1.05±0.14 vs 1.72±0.35 vs 2.64±0.55)依次升高(F=440.557,P<0.05);轻度组、中度组、重度组哮喘患儿外周血LncRNA CASC7的表达水平(0.78±0.09 vs 0.67±0.09 vs 0.44±0.08)依次降低(F=140.168,P<0.05),而miR-21的表达水平(2.34±0.32 vs 2.63±0.53 vs 2.98±0.42)依次升高(F=20.625,P<0.05);Pearson相关性分析显示,支气管哮喘急性发作期患儿外周血LncRNA CASC7与hs-CRP、TNF-α、IL-6的水平呈负相关(r分别为-0.423、-0.375、-0.398,P均<0.05),而与FEV1、FVC、PEF测定值呈正相关(r分别为0.321、0.453、0.441,P均<0.05);miR-21与hs-CRP、TNF-α、IL-6水平呈正相关(r分别为0.479、0.501、0.373,P均<0.05),而与FEV_(1)、FVC、PEF测定值呈负相关(r分别为-0.334、-0.496、-0.505,P均<0.05);LncRNA CASC7与miR-21的表达呈负相关(r=-0.568,P<0.05);LncRNA CASC7鉴别诊断支气管哮喘急性发作期与缓解期的ROC曲线下面积(AUC^(ROC))为0.863(95%CI:0.809~0.906),当cut-off值为0.76时,其敏感性为78.26%,特异性82.47%;miR-21鉴别诊断二者的AUC^(ROC)为0.922(95%CI:0.877~0.954),当cut-off值为2.14时,其敏感性为82.61%,特异性为83.51%;二者联合检测鉴别诊断二者的AUC^(ROC)为0.955(95%CI:0.918~0.979),敏感性为89.57%,特异性为88.66%。结论 支气管哮喘急性发作期患儿外周血中LncRNA CASC7水平下调,miR-21水平上调,且与病情严重程度有关;二者联合可作为支气管哮喘急性发作期的潜在辅助诊断指标。展开更多
文摘BACKGROUND Hepatocellular carcinoma(HCC)is a common malignant gastrointestinal tumor.There are currently few clinical diagnostic and prognostic markers for HCC.LncRNA cancer susceptibility candidate 9(CASC9)is a long-chain non-coding RNA discovered in recent years,and previous studies have found that lncRNA CASC9 participates in the occurrence and development of HCC,but its clinical value remains unclear.AIM To determine the expression of lncRNA CASC9 in HCC and its diagnostic and prognostic value.METHODS Data on CASC9 expression in patients with HCC were collected from the Cancer Genome Atlas(TCGA)database to analyze the relationship between CASC9 and patient survival.A total of 80 HCC patients treated in The First Affiliated Hospital of Guangxi Medical University from May 2012 to January 2014 were enrolled in the patient group,and 50 healthy subjects were enrolled in the control group during the same period.CASC9 expression in the two groups was determined using quantitative real-time polymerase chain reaction,and its diagnostic and prognostic value was analyzed based on the CASC9 data and pathological data in these HCC patients.The relationship between CASC9 and patient survival was assessed during the 5-year follow-up period.RESULTS Analysis of data from TCGA database revealed that control samples showed significantly lower CASC9 expression than carcinoma tissue samples(P<0.001);the low CASC9 expression group had a higher survival rate than the high CASC9 expression group(P=0.011),and the patient group showed significantly increased expression of serum CASC9,with the area under the curve(AUC)of 0.933.CASC9 expression was related to tumor size,combined hepatitis,tumor,node,metastasis(TNM)staging,lymph node metastasis,differentiation and alpha fetoprotein,and the high CASC9 expression group showed lower 1-year,3-year and 5-year survival rates than the low CASC9 expression group(all aP<0.05).Multivariate Cox regression analysis revealed that TNM staging,lymph node metastasis,differentiation,alpha fetoprotein and CASC9 were independent factors affecting the prognosis of patients.Stage I+II patients with lymph node metastasis,low differentiation,and alpha fetoprotein>200 ng/mL had a poor 5-year survival rate.CONCLUSION High CASC9 expression is beneficial in the prognosis of HCC patients.CASC9 is expected to be a potential diagnostic and prognostic indicator of HCC.
文摘目的 检测长链非编码RNA(LncRNA)癌易感性候选基因7(CASC7)、微小RNA-21(miR-21)在支气管哮喘急性发作期患儿外周血中的表达水平并分析其临床意义。方法 选取2020年6月至2021年10月在襄阳市中心医院就诊的支气管哮喘急性发作期患儿115例(急性发作期组)以及支气管哮喘缓解期患儿97例(缓解期组),根据病情程度将急性发作期组患儿分为轻度组45例,中度组39例,重度组31例。另收集同期体检健康儿童100例(健康人对照组)。采用实时荧光定量PCR法(RT-qPCR)检测各组外周血LncRNA CASC7、miR-21的表达水平;检测第1秒用力呼气容积(forced expiratory volume in 1 second, FEV1)、用力肺活量(forced vital capacity, FVC)、最大呼吸峰流速(peak expiratory flow, PEF)等肺功能指标;采用免疫比浊法检测超敏C反应蛋白(hs-CRP)的表达,ELISA法检测TNF-α、IL-6的表达水平;Pearson法分析LncRNA CASC7、miR-21的表达与hs-CRP、TNF-α、IL-6及FEV1、FVC、PEF的相关性,并进一步分析LncRNA CASC7与miR-21的相关性;ROC曲线分析LncRNA CASC7、miR-21对支气管哮喘急性发作期患儿的鉴别诊断价值。结果 RT-qPCR检测结果显示,健康人对照组、急性发作期组、缓解期组患儿外周血LncRNA CASC7的表达水平(1.09±0.18 vs 0.93±0.12 vs 0.65±0.10)依次降低(F=289.447,P<0.05),miR-21的表达水平(1.05±0.14 vs 1.72±0.35 vs 2.64±0.55)依次升高(F=440.557,P<0.05);轻度组、中度组、重度组哮喘患儿外周血LncRNA CASC7的表达水平(0.78±0.09 vs 0.67±0.09 vs 0.44±0.08)依次降低(F=140.168,P<0.05),而miR-21的表达水平(2.34±0.32 vs 2.63±0.53 vs 2.98±0.42)依次升高(F=20.625,P<0.05);Pearson相关性分析显示,支气管哮喘急性发作期患儿外周血LncRNA CASC7与hs-CRP、TNF-α、IL-6的水平呈负相关(r分别为-0.423、-0.375、-0.398,P均<0.05),而与FEV1、FVC、PEF测定值呈正相关(r分别为0.321、0.453、0.441,P均<0.05);miR-21与hs-CRP、TNF-α、IL-6水平呈正相关(r分别为0.479、0.501、0.373,P均<0.05),而与FEV_(1)、FVC、PEF测定值呈负相关(r分别为-0.334、-0.496、-0.505,P均<0.05);LncRNA CASC7与miR-21的表达呈负相关(r=-0.568,P<0.05);LncRNA CASC7鉴别诊断支气管哮喘急性发作期与缓解期的ROC曲线下面积(AUC^(ROC))为0.863(95%CI:0.809~0.906),当cut-off值为0.76时,其敏感性为78.26%,特异性82.47%;miR-21鉴别诊断二者的AUC^(ROC)为0.922(95%CI:0.877~0.954),当cut-off值为2.14时,其敏感性为82.61%,特异性为83.51%;二者联合检测鉴别诊断二者的AUC^(ROC)为0.955(95%CI:0.918~0.979),敏感性为89.57%,特异性为88.66%。结论 支气管哮喘急性发作期患儿外周血中LncRNA CASC7水平下调,miR-21水平上调,且与病情严重程度有关;二者联合可作为支气管哮喘急性发作期的潜在辅助诊断指标。
