Changes of Organic Matter, N, P and K Content of Soils in Red Soil Areas Under Long-Term Experiment

The content of organic matter （OM）, nitrogen （N）, phosphorus （P）, and potassium （K） in the three selected soils, including Calcaric Purpli-Orthic Primosols （purple sand-shale parent material）, Dystric Turbi-Anthric Primosols （quaternary red clay parent material） and Typic Udi-Sandic Primosols （granite parent material） were studied under a long-term experiment by using crop straw and inorganic fertilizers at the Hunan Red Soil Experiment Station of Chinese Academy of Agricultural Sciences. The results showed that the contents of OM, N, P and K in the three selected soils increased after 23 years application of crop straw and inorganic fertilizers, but the contents increased much less when crop straw or inorganic fertilizers was applied alone. The nutrient contents in the three soils developed from granite changed more remarkably than those in the soil derived from quaternary red clay and purple sand-shale. It was also found that the contents of OM, N, and P increased slightly in the treatments without applying fertilizers or returning the crop straw to the root bed. Combined application of inorganic fertilizers and crop straw could remarkably increase the contents of OM, alkalihydrolyzable N and available K, the positive correlation between application of organic fertilizers and increase of OM in soil did not always happened, it provided evidence for the relation between appropriate C/N ratio and accumulation of OM in soil. The increase of nutrient content was influenced by the soil properties. By comparing the contents of nutrient in 0-20 cm depth in the three different soils, it was concluded that the most increases of OM, alkali-hydrolyzable N, and available P were observed in Typic Udi-Sandic Primosols with the average increase by 3.03, 27.38, and 21.73 mg kg^-1, respectively. The available K increased in Dystric Turbi-Anthric Primosols with the average increase by 25.82 mg kg^-1, while it decreased in Calcaric Purpli-Orthic Primosols and Typic Udi-Sandic Primosols. It was concluded that the application of inorganic fertilizer and crop straw was important to improve the soil fertility for all of three selected soils. The straw return to the field had played a significant role for enhancement of the soil quality in the study areas. The application of inorganic fertilizer combined with the straw return to the fields could remarkably improve the soil fertility.

Crop yields and soil organic carbon dynamics in a long-term fertilization experiment in an extremely arid region of northern Xinjiang, China

A long-term fertilization experiment was set up in northern Xinjiang, China to evaluate the dynamics of crop production and soil organic carbon （SOC） from 1990 to 2012 with seven fertilization treatments. The seven treatments included an unfertilized control （CK） and six different combinations of phosphorus （P）, potassium （K）, nitrogen （N）, straw （S） and animal manure （M）. The balanced fertilization treatments had significantly （P〈0.05） higher average yields than the unbalanced ones. The treatment with 2/3 N from potassium sulfate （NPK） and 1/3 N from farmyard manure （NPKM） had a higher average yield than the other treatments. The average yields （over the 23 years） in the treatments of NPK, and urea, calcium superphosphate （NP） did not differ significantly （P〉0.05） but were higher than that in the treatment with urea and potassium sulfate （NK; P〈0.05）. The results also show that the highest increases in SOC （P〈0.05） occurred in NPKM with a potential increase of 1.2 t C/（hm2.a）. The increase in SOC was only 0.31, 0.30 and 0.12 t C/（hm2.a） for NPKS （9/10 N from NPK and 1/10 N from straw）, NPK and NP, respectively; and the SOC in the NP, NK and CK treatments were approaching equilibrium and so did not rise or fall significantly over the 23-year experiment. A complete NPK plus manure fertilization program is recommended for this extremely arid region to maximize both yields and carbon sequestration.

The principles and guidelines for designing long-term agronomic experiments

Many of the important questions facing farming systems in the world today require long-term studies to provide meaningful information and answers. A long-term agronomic experiment (LTAE) should (1) have long-term objectives; (2) study important soil processes or ecological processes; and (3) be related to the productivity and sustainability of systems. A well established LTAE can provide both insights into how the system operates and foresight into where the system goes. The prerequisites for setting up a LTAE are the secured land, continuous funding and dedicated scientists. A number of principles must be considered carefully when establishing a LTAE, (1) the site must be representative of large areas; (2) the treatments should be simple, but focusing on the big questions; (3) the plots should be large enough to allow subsequent modification of the experiment if this becomes necessary; (4) crop rotations should minimise, wherever possible, the risk of build-up of pests and diseases, and rotational phase should be considered in a rotational experiment; (5) a clearly defined experimental protocol should be developed to ensure data collected is scientifically valid and statistically analysable, but with flexibility to allow essential changes; (6) soil samples, possibly plant samples, should be achieved to provide better answer to the original questions when new, perhaps more accurate analytical techniques are developed, or answer new research questions that were not considered in the original design. The MASTER experiment in Australia was used as a case study to demonstrate how these principles are implemented in practice.

Long-Term Survival in Traumatic Brain Injury and Near-Death Experience Increases Suicide Risks: A Personal Experience and Related Literature

Background: Traumatic brain injury (TBI) remains a cause of lifelong disability, death, and suicide worldwide. TBI-induced near-death experience (NDE) could increase suicide risks. Objective: We investigated TBI coupled with NDE and posttraumatic stress disorder (PTSD) as a possible indicator of suicide. Methods: A 17-year-old male who sustained an acute severe TBI in a traffic collision, was comatose 14 days, had an NDE awakening from the coma, and, years after rehabilitation, suffered PTSD, clinical depression, and survived a suicide attempt. This personal experience of a TBI-induced NDE and lingering PTSD was acquired directly from the patient by interview. We discuss his case while considering relevant literature. Results: Longitudinal data from 1961 to 2021 generated from the PubMed interface revealed 4056 TBI patients committed suicide. NDE was only reported in one of those cases and, although not a suicide, in the personal experience. Neuropsychological assessment at long-term follow-ups revealed few TBI patients exhibited normal mental/physical functions compared to the general population. Unfavorable GOS scores were risk predictors for neuropsychological/physical impairments later in life, with outcomes of depression, PTSD, poor QOL, and/or suicide. Conclusions: For TBI-NDE survivors, including those with PTSD, long-term periodic neuropsychological follow-ups and psychosocial support may help decrease suicide risks.

Long-Term Effect of Fertilizer and Rice Straw on Mineral Composition and Potassium Adsorption in a Reddish Paddy Soil

Increasing K＋ adsorption can be an effective alternative in building an available K pool in soils to optimize crop recovery and minimize losses into the environment. We hypothesized that long-term fertilization might change K＋ adsorption because of changes in the chemical and mineralogical properties of a rice （Oryza sativa L.）. The aims of this study were （i） to determine clay minerals in paddy soil clay size fractions using X-ray diffraction methods and a numerical diagramdecomposition method; （ii） to measure K＋ adsorption isotherms before and after H202 oxidation of organic matter, and （iii） to investigate whether K＋ adsorption is correlated with changes in soil chemical and mineral properties. The 30-yr longterm fertilization treatments caused little change in soil organic C （SOC） but a large variation in soil mineral composition. The whole-clay fraction （〈5 Jam） corresponded more to the fertilization treatment than the fine-clay fraction （〈1 gin） in terms of percentage of illite peak area. The total percentage of vermiculite-chlorite peak area was significantly negatively correlated with the total percentage ofillite peak area in the 〈5 lam soil particles （R=-0.946, P〈0.0006）. Different fertilization treatments gave significantly different results in K＋ adsorption. The SOC oxidation test showed positive effects of SOC on K＋ adsorption at lower K＋ concentration （≤120 mg L-0 and negative effects at higher K＋ concentration （240 mg L-l）. The K＋ adsorption by soil clay minerals after SOC oxidization accounted for 60-158% of that by unoxidized soils, suggesting a more important role of soil minerals than SOC on K＋ adsorption. The K＋ adsorption potential was significantly correlated to the amount of poorly crystallized illite present （R--0.879, P=0.012）. The availability of adsorbed K＋ for plant growth needs further study.

Effects of Long-Term Winter Planted Green Manure on Physical Properties of Reddish Paddy Soil Under a Double-Rice Cropping System

Soil physical properties are important indicators of the potential for agricultural production.Our objective was to evaluate the effects of long-term inputs of green manures on physical properties of a reddish paddy soil（Fe-Typic Hapli-Stagnic Anthrosols） under a double cropping system.The common cropping pattern before the study was early-late rice-fallow（winter）.The field treatments included rice-rice-fallow（R-R-WF）,rice-rice-rape（R-R-RP）,rice-rice-Chinese milk vetch（RR-MV）,and rice-rice-ryegrass（R-R-RG）.The rape,Chinese milk vetch and ryegrass were all incorporated as green manures 15 d before early rice transplanting during the following year.The soil bulk density in all green manure treatments was significantly reduced compared with the winter fallow treatment.Soil porosity with green manure applications was significantly higher than that under the winter fallow.The green manure treatments had higher 0.25-5 mm water stable aggregates and aggregates stabilities in the plow layer（0-15 cm depth） compared with the fallow treatment.The mean weight diameter（MWD） and normalized mean weight diameter（NMWD） of aggregates in the green manure treatment were larger than that with the winter fallow.Soil given green manure retained both a higher water holding capacity in the plow layer soil,and a larger volume of moisture at all matric potentials（-10,-33 and-100 kPa）.We conclude that the management of double-rice fields in southern central China should be encouraged to use green manures along with chemical fertilizers to increase SOC content,improve soil physical properties and soil fertility.

Effects of Long-Term Combined Application of Organic and Mineral Fertilizers on Microbial Biomass,Soil Enzyme Activities and Soil Fertility

Soil health is important for the sustainable development of terrestrial ecosystem. In this paper, we studied the relationship between soil quality and soil microbial properties such as soil microbial biomass and soil enzyme activities in order to illustrate the function of soil microbial properties as bio-indicators of soil health. In this study, microbial biomass C and N contents （Cmic ＆ Nmic）, soil enzyme activities, and soil fertility with different fertilizer regimes were carried out based on a 15-year long-term fertilizer experiment in Drab Fluvo-aquic soil in Changping County, Beijing, China. At this site, 7 different treatments were established in 1991. They were in a wheat-maize rotation receiving either no fertilizer （CK）, mineral fertilizers （NPK）, mineral fertilizers with wheat straw incorporated （NPKW）, mineral fertilizers with incremental wheat straw incorporated （NPKW＋）, mineral fertilizers plus swine manure （NPKM）, mineral fertilizers plus incremental swine manure （NPKM＋） or mineral fertilizers with maize straw incorporated （NPKS）. In different fertilization treatments Cmic changed from 96.49 to 500.12 mg kg^-1, and Nmic changed from 35.89 to 101.82 mg kg^-1. Compared with CK, the other treatments increased Cmic ＆ Nmic, Cmic/Corg （organic C） ratios, Cmic/Nmic, urease activity, soil organic matter （SOM）, soil total nitrogen （STN）, and soil total phosphorus （STP）. All these properties in treatment with fertilizers input NPKM＋ were the highest. Meantime, long-term combined application of mineral fertilizers with organic manure or crop straw could significantly decrease the soil pH in Fluvo-aquic soil （the pH around 8.00 in this experimental soil）. Some of soil microbial properties （Cmic/Nmic, urease activity） were positively correlated with soil nutrients. Cmic/Nmic was significantly correlated with SOM and STN contents. The correlation between catalase activity and soil nutrients was not significant. In addition, except of catalase activity, the soil pH in this experiment was negatively correlated with soil microbial properties. In conclusion, soil microbial properties reflect changes of soil quality and thus can be used as bio-indicators of soil health.

Discrepancy in Response of Rice Yield and Soil Fertility to Long-Term Chemical Fertilization and Organic Amendments in Paddy Soils Cultivated from Infertile Upland in Subtropical China

From 1990,over 17 years field experiment was carried out in paddy field cultivated from infertile upland to evaluate the response of rice productivity,soil organic carbon（SOC）,and total N to long-term NPK fertilization or NPK combined with organic amendments.The field trials included NPK（N,P,K fertilizer）,NPKRS（NPK combined with rice straw）,NPK2RS（NPK combined with double amount of rice straw）,NPKPM（NPK combined with pig manure） and NPKGM（NPK combined with green manure） and the cropping system was rice-rice（Oryza sativa L.） rotation.Annual rice yield,straw biomass,and harvesting index increased steadily with cultivation time in all treatments.Average annual rice yield from 1991 to 2006 was ranged from 7 795 to 8 572 kg ha-1 among treatments.Rice yields in treatments with organic amendments were usually higher than that in treatment with NPK.Contents of SOC and total N also increased gradually in the cultivation years and reached the level of 7.82 to 9.45 and 0.85 to 1.03 g kg-1,respectively,in 2006.Soil fertilities in treatments with chemical fertilization combined with organic amendments were relatively appropriate than those in treatment with NPK.There was obvious discrepancy between cumulative characters of rice yield and soil organic fertility in newly formed paddy field.Compared with relatively high rate of crop productivity improvement,cumulative rates of SOC and total N were much lower in our study.SOC and total N contents were still less than half of those in local highly productive paddy soils after 17 years cultivation in subtropical China.Present work helps to better understand the development of infertile paddy soils and to estimate the potential of yield improvement in this region.

Short-and long-term behaviors of drifts in the Callovo-Oxfordian claystone at the Meuse/Haute-Marne Underground Research Laboratory

Since 2000, the French National Radioactive Waste Management Agency （ANDRA） has been constructing an Underground Research Laboratory （URL） at Bure （east of the Paris Basin） to perform experiments in order to obtain in situ data necessary to demonstrate the feasibility of geological repository in the Callovo- Oxfordian claystone. An important experimental program is planned to characterize the response of the rock to different drift construction methods, Before 2008, at the main level of the laboratory, most of the drifts were excavated using pneumatic hammer and supported with rock bolts, sliding steel arches and fiber shotcrete. Other techniques, such as road header techniques, stiff and flexible supports, have also been used to characterize their impacts. The drift network is developed following the in situ major stresses. The parallel drifts are separated enough so as they can be considered independently when their hydromechanical （HM） behaviors are compared. Mine-by experiments have been performed to measure the HM response of the rock and the mechanical loading applied to the support system due to the digging and after excavation. Drifts exhibit extensional （mode I） and shear fractures （modes II and III） induced by excavation works. The extent of the induced fracture networks depends on the drift orientation versus the in situ stress field. This paper describes the drift convergence and deformation in the surrounding rock walls as function of time and the impact of different support methods on the rock mass behavior. An observation based method is finally applied to distinguish the instantaneous and time-dependent parts of the rock mass deformation around the drifts.

Effects of Long-Term Organic Amendments on Soil Organic Carbon in a Paddy Field: A Case Study on Red Soil

Soil organic carbon （SOC） is one of the main carbon reservoirs in the terrestrial ecosystem. It is important to study SOC dynamics and effects of organic carbon amendments in paddy fields because of their vest expansion in south China. A study was carried out to evaluate the relationship between the SOC content and organic carbon input under various organic amendments at a long-term fertilization experiment that was established on a red soil under a double rice cropping system in 1981. The treatments included non-fertilization （CK）, nitrogen-phosphorus-potassium fertilization in early rice only （NPK）, green manure （Astragalus sinicus L.） in early rice only （OM1）, high rate of green manure in early rice only （OM2）, combined green manure in early rice and farmyard manure in late rice （OM3）, combined green manure in early rice, farmyard manure in late rice and rice straw mulching in winter （OM4）, combined green manure in early rice and rice straw mulching in winter （OMS）. Our data showed that the SOC content was the highest under OM3 and OM4, followed by OM1, OM2 and OM5, then NPK fertilization, and the lowest under non-fertilization. However, our analyses in SOC stock indicated a significant difference between OM3 （33.9 t ha^-1） and OM4 （31.8 t ha^-1）, but no difference between NPK fertilization （27 t ha^-1） and nonfertilization （28.1 t ha^-1）. There was a significant linear increase in SOC over time for all treatments, and the slop of linear equation was greater in organic manure treatments （0.276-0.344 g kg-1 yr^-1） than in chemical fertilizer （0.216 g kg^-1 yr^-1） and no fertilizer （0.127 g kg^-1 yr^-1）.

Experimental study on rheological deformation and stress properties of limestone

The systematic experiment regarding the general uniaxial compression test and the creep deformations of the typical limestones from the surrounding rock of the highway tunnels were made.The relationship between the axial stress and the delayed deformation steady value was obtained from the creep tests under low loading stresses.By the least square method,the parameters of Nishihara creep model were calculated from the creep curves.The results indicate that the strain change always lags behind the increase of stress,and the long-term strength of the limestone is about 80.6% of the stress at the volumetric strain reversal which is obtained from the conventional uniaxial compression test.

Effects of long-term fertilization on soil gross N transformation rates and their implications

Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates. The results showed that if chemical fertilizers of N, P and K were applied in balance, soil p H decreased very slightly. There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment（the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals（CI） with a mean of 1.23）, mainly due to the increasing soil total N content. The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment. However, long-term chemical fertilizer application did not significantly affect the gross NH4＋ immobilization rate, but accelerated gross nitrification rate（1.19; 95% CI： 1.08 to 1.31）. Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment. Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.

Integrating beneficiaries into assessment of ecosystem services from managed forests at the Hubbard Brook Experimental Forest,USA

Background：Forests contribute to human wellbeing through the provision of important ecosystem services.Methods：In this study,we investigated how the perceived importance of ecosystem services may impact the overall benefit provided by managed watersheds at the Hubbard Brook Experimental Forest over a 45-year period,using standardized measures of service capacity weighted by service importance weights derived from a survey of beneficiaries.Results：The capacity of watersheds to regulate water flow and quality was high in all watersheds throughout the study period,whereas cultural services such as scenic beauty declined after harvest.Impacts on greenhouse gas regulation depended on the efficiency with which harvested biomass was used.Surveys revealed that stakeholders placed high value on all ecosystem services,with regulating and cultural services seen as more important than provisioning services.When service metrics were weighted by survey responses and aggregated into a single measure,total service provision followed the same overall trend as greenhouse gas regulation.Where biomass use was less efficient in terms of greenhouse gas emissions,harvesting resulted in an overall ＂ecosystem service debt＂;where use was more efficient,this ＂ecosystem service debt＂ was reduced.Beneficiaries＇ educational backgrounds significantly affected overall assessment of service provision.Beneficiaries with college or university degrees incurred smaller ＂ecosystem service debts＂ and were less negatively affected by harvesting overall.Conclusions：This study highlights the importance of including empirical measures of beneficiary preference when attempting to quantify overall provision of ecosystem services to human beneficiaries over time.

Effects of Long-Term Fertilization on Distribution of Organic Matters and Nitrogen in Cinnamon Soil Macro-Aggregates

Soil samples collected from a 25-year long-term fertilizer experiment carried out on the Earth-cumulic-Orthic Anthrosols in semi-humid farmland ecological system, were used to study the distribution of soil organic matters, total nitrogen, nitrate nitrogen, and ammoniate nitrogen in different grades of soil macro-aggregates in order to study the effects of long-term application of organic manures in combination with chemical fertilizers. It is showed that the percentage of mass of the soil macro-aggregates with long-term application of fertilizers with sizes of 5-2 mm is increased compared with that of the samples with no fertilizer. It is easier to form lager size soil macro-aggregates by the long-term application of organic manures in combination with chemical fertilizers. The contents of organic matters, total nitrogen and nitrate nitrogen are all higher after treatments with different combinations of fertilizers, while there is a little effect on the contents of ammoniate nitrogen. The contents of organic matters, total nitrogen in the grades of soil macro-aggregates from the plough layers of the treated farmland exhibited significant difference. Moreover, the contents of organic matters and total nitrogen in the soil macro-aggregates with the size of 1-0.25 mm is the highest in all treated soil samples. The contents of nitrate nitrogen in soil macro-aggregates increased with the increasing of soil macro-aggregate size except those applied with chemical fertilizer and lower amount of corn stover. The results of correlation analysis revealed that there exists a significantly positive correlation between the percentage of mass of soil macro-aggregates with the size of 5-2 mm and the contents of organic matters, total nitrogen and nitrate nitrogen in the soil samples. However, the correlation between the percentage of mass of soil macro-aggregates with the size of 1-0.25 rnm and the contents of total nitrogen and nitrate nitrogen is significantly negative.The study showed that the highest contributing rates of macro-aggregates fractions to soil fertility is from the soil macro-aggregates fraction with the size of 1-0.25 mm in most of the cases.

Nitrogen mobility,ammonia volatilization,and estimated leaching loss from long-term manure incorporation in red soil

Nitrogen（N） loss from fertilization in agricultural fields has an unavoidable negative impact on the environment and a better understanding of the major pathways can assist in developing the best management practices. The aim of this study was to evaluate the fate of N fertilizers applied to acidic red soil（Ferralic Cambisol） after 19 years of mineral（synthetic） and manure fertilizer treatments under a cropping system with wheat-maize rotations. Five field treatments were examined： control（CK）, chemical nitrogen and potash fertilizer（NK）, chemical nitrogen and phosphorus fertilizer（NP）, chemical nitrogen, phosphorus and potash fertilizer（NPK） and the NPK with manure（NPKM, 70% N from manure）. Based on the soil total N storage change in 0–100 cm depth, ammonia（NH_3） volatilization, nitrous oxide（N_2O） emission, N plant uptake, and the potential N leaching loss were estimated using a mass balance approach. In contrast to the NPKM, all mineral fertilizer treatments（NK, NP and NPK） showed increased nitrate（NO_3~–） concentration with increasing soil depth, indicating higher leaching potential. However, total NH_3 volatilization loss was much higher in the NPKM（19.7%） than other mineral fertilizer treatments（≤4.2%）. The N_2O emissions were generally low（0.2–0.9%, the highest from the NPKM）. Total gaseous loss accounted for 1.7, 3.3, 5.1, and 21.9% for NK, NP, NPK, and NPKM treatments, respectively. Estimated N leaching loss from the NPKM was only about 5% of the losses from mineral fertilizer treatments. All data demonstrated that manure incorporation improved soil productivity, increased yield, and reduced potential leaching, but with significantly higher NH_3 volatilization, which could be reduced by improving the application method. This study confirms that manure incorporationis an essential strategy in N fertilization management in upland red soil cropping system.

QUICK ASSESSMENT METHODOLOGY FOR RELIABILITY OF SOLDER JOINTS IN BALL GRID ARRAY(BGA)ASSEMBLY——PART Ⅱ:RELIABILITY EXPERIMENT AND NUMERICAL SIMULATION

In the present study,a facility,i.e.,a mechanical deflection system (MDS),was established and applied to assess the long-term reliability of the solder joints in plastic ball grid array (BGA) assembly.It was found that the MDS not only quickly assesses the long-term reliability of solder joints within days,but can also mimic similar failure mechanisms in accelerated thermal cycling (ATC) tests. Based on the MDS and ATC reliability experiments,the acceleration factors (AF) were obtained for different reliability testing conditions.Furthermore,by using the creep constitutive relation and fatigue life model developed in part I,a numerical approach was established for the purpose of virtual life prediction of solder joints. The simulation results were found to be in good agreement with the test results from the MDS.As a result,a new reliability assessment methodology was established as an alternative to ATC for the evaluation of long-term reliability of plastic BGA assembly.

The efficiency of long-term straw return to sequester organic carbon in Northeast China＇s cropland

Black soil is one of the most precious soil resources on earth because it has abundant carbon stocks and a relatively high production capacity. However, decreasing organic matter after land reclamation, and the effects of long-term inputs of organic carbon have made it less fertile black soil in Northeast China. Straw return could be an effective method for improving soil organic carbon（SOC） sequestration in black soils. The objective of this study was to evaluate whether straw return effectively increases SOC sequestration. Long-term field experiments were conducted at three sites in Northeast China with varying latitudes and SOC densities. Study plots were subjected to three treatments： no fertilization（CK）; inorganic fertilization（NPK）; and NPK plus straw return（NPKS）. The results showed that the SOC stocks resulting from NPKS treatment were 4.0 and 5.7% higher than those from NPK treatment at two sites, but straw return did not significantly affect the SOC stocks at the third site. Furthermore, at higher SOC densities, the NPKS treatment resulted in significantly higher soil carbon sequestration rates（CSR） than the NPK treatment. The equilibrium value of the CSR for the NPKS treatment equated to cultivation times of 17, 11, and 8 years at the different sites. Straw return did not significantly increase the SOC stocks in regions with low SOC densities, but did enhance the C pool in regions with high SOC densities. These results show that there is strong regional variation in the effects of straw return on the SOC stocks in black soil in Northeast China. Additional cultivations and fertilization practices should be used when straw return is considered as an approach for the long-term improvement of the soil organic carbon pool.

Long-term effects of management systems on crop yield and soil physical properties of semi-arid tropics of Vertisols

Long-term experiments can be used to assess management induced changes in soil properties and sustainability of the management system in terms of the productivity. Such data are scanty, especially in the semi-arid tropics (SAT) region. A long-term experiment established in 1976 at ICRISAT in India on Vertisols with two management treatments;improved management (IM), comprising semi-permanent broadbed and furrow (BBF) landform with minimum tillage and improved cropping practices;and traditional management (TM) system comprising keeping the land fallow during the rainy season and sowing on flat landform during post-rainy season with traditional cropping practices, was sampled after 24 and 34 years for soil physical and hydrological properties. Results showed that both in short-and long-term the management systems had profound effect on crop yields. Also in the long-term IM and TM management systems had significant effect on several soil physical and hydrological properties. Throughout the soil profile IM systems had significantly lower bulk density, significantly higher porosity, substantially lower penetration resistance both at 5 cm (1 and 8 MPa) and 15 cm depths (8 and 15 MPa), significantly higher infiltration and sorptivity and significantly larger mean weight diameter of 4.3 mm compared to 2.8 mm for soils under TM. However, management systems had no significant effect on moisture holding capacities both at 0.033 and 1.5 MPa. Significant differences between the improved and traditional systems were observed in the size and pattern of soil surface cracks. Over the long-term, the improved management systems has very favorable effects on soil physical and hydrological properties and on the soil surface cracking and its patterns, thereby contributing to higher productivity.

长期模拟失重实验中医证候诊断量表的研究

目的制订适用于长期模拟失重实验受试者整体健康状态评价的中医证候诊断量表,并对其信度与效度进行初步评价。方法本研究基于对长期航天飞行不同阶段中的人体中医病机的认识,通过文献调研结合专家咨询的方法制订量表。采用重测信度和内部一致性系数来检验量表信度;进行为期90 d的头低位卧床实验,采用项目分析和因子分析法评价量表的效度,采用旋转成分矩阵分析各条目与维度间的相关性。结果长期模拟失重实验中医证候诊断量表包含8个维度、57个条目。信度分析结果:30名受试者前后两次量表评分总分相关系数为0.889,表明量表的稳定性很高;量表的克龙巴赫α系数为0.934,表明量表内部一致性非常好;分半信度校正后为0.858,表明量表的信度很高。效度分析结果:57个条目在高分组和低分组的差异均有统计学意义(P<0.01),各条目与量表总分的皮尔逊相关系数均>0.4(P<0.001);采用主成分因子分析法提取8个公因子,累积贡献率为55.293%;旋转成分矩阵分析结果经医理分析和专家反馈显示,与初始8个维度的重合度高达87.5%,表明量表的结构效度良好。结论量表具有良好的信度和效度,能够进行中医证候诊断,适用于长期模拟失重实验,可为受试者主观感受的评价提供客观量化新方法。

果园红壤供氮水平季节性变化及对长期套种绿肥的响应研究

本研究以柿子(Diospyros kaki)园为对象,研究了长期(近20年)套种豆科绿肥对土壤可溶性氮和氮水解酶季节性变化的影响。试验设3个处理:套种平托花生(Arachis pintoi)(TPA)、套种圆叶决明(Chamaecrista rotundifolia)(TPC)和清耕果园(CK)。结果表明:长期套种2种豆科绿肥没有改变果园红壤供氮水平的季节性变化规律,6月各处理硝态氮(NO_(3)^(-)-N)、可溶性总氮(Total soluble nitrogen,TSN)和可溶性有机氮(Soluble organic nitrogen,SON)含量最高,分别为17.71~21.81,31.40~75.12和7.77~47.65 mg·kg^(-1);12月NO_(3)^(-)-N,TSN和SON含量最低,分别为4.88~6.02,15.17~17.45和5.03~7.92 mg·kg^(-1)。3月、6月和8月铵态氮(NH_(4)^(+)-N)含量高于4月、10月和12月;6月4种氮水解酶活性最高,8月和12月活性最低。长期套种2种豆科绿肥显著提高了生长季3—8月NO_(3)^(-)N,TSN,SON和NH_(4)^(+)-N含量以及蛋白酶、天冬酰胺酶、脲酶和谷氨酰胺酶活性。因此,长期套种圆叶决明和平托花生是通过增加生长季的可溶性氮含量和氮水化酶活性来增加土壤氮供应。