Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species

Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.

Securing offshore resources development:A mathematical investigation into gas leakage in long-distance flexible pipes

Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexible pipes and further diffuse into the annular space,incurring annular pressure build-up and/or production of acidic environment,which poses serious challenges to the structure and integrity of the flexible pipes.Gas permeation in pipes is a complex phenomenon governed by various factors such as internal pressure and temperature,annular structure,external temperature.In a long-distance gas flexible pipe,moreover,gas permeation exhibits non-uniform features,and the gas permeated into the annular space flows along the metal gap.To assess the complex gas transport behavior in long-distance gas flexible pipes,a mathematical model is established in this paper considering the multiphase flow phenomena inside the flexible pipes,the diffusion of gas in the inner liner,and the gas seepage in the annular space under varying permeable properties of the annulus.In addition,the effect of a variable temperature is accounted.A numerical calculation method is accordingly constructed to solve the coupling mathematical equations.The annular permeability was shown to significantly influence the distribution of annular pressure.As permeability increases,the annular pressure tends to become more uniform,and the annular pressure at the wellhead rises more rapidly.After annular pressure relief followed by shut-in,the pressure increase follows a convex function.By simulating the pressure recovery pattern after pressure relief and comparing it with test results,we deduce that the annular permeability lies between 123 and 512 m D.The results help shed light upon assessing the annular pressure in long distance gas flexible pipes and thus ensure the security of gas transport in the emerging development of offshore resources.

Root canal therapy combined with endoscopic sinus surgery for odontogenic sinusitis:Efficacy comparison in a cohort study

BACKGROUND Odontogenic maxillary sinusitis,often triggered by dental issues like periapical periodontitis,significantly contributes to chronic sinusitis,mainly affecting adults around 50 years old,emphasizing the need for a multidisciplinary diagnostic and treatment approach.AIM To investigate the therapeutic effect and clinical value of root canal therapy combined with nasal endoscopic surgery compared with simple root canal the-rapy in the treatment of severe odontogenic maxillary sinusitis caused by peria-pical periodontitis.METHODS The clinical data,diagnosis,and treatment of 200 patients with severe odonto-genic maxillary sinusitis caused by periapical periodontitis from October 2020 to October 2021 were analyzed retrospectively.Among them,63 patients were treated with simple root canal therapy as the control group,and 137 patients were treated with root canal therapy combined with nasal endoscopic surgery as the observation group.The therapeutic effect,Lund-Kennedy endoscopic score,paranasal sinus Lund-Mackay score,complication rate,recurrence rate,and patient satisfaction were compared between the two groups.RESULTS First,we compared the effective rates:23 cases were cured,22 were improved,and 8 were ineffective in the control group,yielding a total effective rate of 84.90%.Meanwhile,97 cases were cured,34 improved,and 6 were ineffective in the observation group,resulting in a total effective rate of 95.62%.The observation group had a higher total effective rate compared with the control group(P<0.05).Second,we compared the Lund–Kennedy endoscopic score.Before treatment,no significant difference(P>0.05)was observed in this score between the two groups.After treatment,the Lund–Kennedy endoscopic score decreased in both groups.The Lund–Kennedy endoscopic score of the observation group at 3 and 6 mo after treatment was lower compared to that of the control group(P<0.05).Third,we compared the Lund–Mackay score of paranasal sinuses.Before treatment,there was no significant difference in this score between the two groups(P>0.05).After treatment,the Lund–Mackay scores of paranasal sinuses decreased in both groups.The Lund–Mackay scores of paranasal sinuses in the observation group at 3 and 6 mo after treatment were lower compared to those of the control group(P<0.05).Fourth,we compared the incidence and recurrence rate of complications.Three months after treatment,no significant difference was found in the incidence and recurrence rate of complications between the observation group(6.56%)and the control group(9.52%)(P>0.05).However,6 mo after treatment,the incidence and recurrence rate of complications in the observation group(2.91%)was significantly higher compared to that of the control group(12.69%)(P<0.05).Fifth,we compared patient satisfaction.Six months after treatment,the patient satisfaction of the observation group(93.43%)was significantly better than that of the control group(84.12%)(P<0.05).CONCLUSION Root canal therapy combined with nasal endoscopic surgery has a good therapeutic effect on severe odontogenic maxillary sinusitis caused by periapical periodontitis,and it can reduce the injury of maxillary sinus mucosa and bone,and significantly reduce the incidence of complications and recurrence rate.Meanwhile,it has high patient satisfaction and remarkable therapeutic effect,which is suggested to be popularized and applied in clinic.

Ecological Design of Flood Discharging Canals in Shijiazhuang City

From the perspective of ecological conservation and satisfaction of people's visual and psychological demands,existing problems in the urban revetment were analyzed,and the connotation of ecological revetment as well as the selection of different ecological revetments according to different types of river ways was discussed.

Effect of restoration technique on resistance to fracture of endodontically treated anterior teeth with flared root canals

This study was designed to compare the impact of post and core systems on resistance to fracture of endodontically treated anterior teeth with flared root canals and to assess their fracture pattern. Sixty central incisors were cut horizontally 2 mm coronal to the cementoenamel junction(CEJ). After root canal therapy, teeth were assigned into 6 groups(n = 10 each) based on a post system and used as follows: Group C, non-flared root received size #1 glass fiber posts(Control); Group AP, flared root restored with anatomical post; Group RC, flared root restored with size #1 fiber post and cemented with thick layer of resin cement; Group CR, flared root restored with size #1 and reinforced with composite resin; Group CM, cast post-core; Group CP, CAD/CAM polymer-infiltrated ceramic post and core.Following post cementation, core build-up and crown insertion, the specimens were thermo-cycled up to 10,000 cycles(5 C/55 C; 30 seconds dwell time, 6 seconds transition time) and then statically loaded at 1 mm/minute crosshead speed using a universal testing machine. One-way ANOVA and Tukey HSD post hoc test(α= 0.05) were used for data analysis. Group C recorded significantly higher resistance to fracture values [(826.9±39.1) N] followed by group CP [(793.8±55.6) N] while group RC yielded the lowest fracture resistance values [(586.7±51.4) N]. The resistance to fracture of wide root canals can be enhanced by using one-piece CAM/CAM post and core as an alternative to the use of either glass fiber post, relined with composite resin increasing the thickness of luting cement or the use of cast post and core system. However, this was an in vitro investigation and further in vivo studies are necessary.

Cone-beam computed tomography analysis of curved root canals after mechanical preparation with three nickel-titanium rotary instruments

Cone beam computed tomography is a 3-dimensional high resolution imaging method. The purpose of this study was to compare the effects of 3 different NiTi rotary instruments used to prepare curved root canals on the final shape of the curved canals and total amount of root canal transportation by using cone-beam computed tomography. A total of 81 mesial root canals from 42 extracted human mandibular molars, with a curvature ranging from 15 to 45 degrees, were selected. Canals were randomly divided into 3 groups of 27 each. After preparation with Protaper, Revo-S and Hero Shaper, the amount of transportation and centering ability that occurred were assessed by using cone beam computed tomography. Utilizing preand post-instrumentation radiographs, straightening of the canal curvatures was determined with a computer image analysis program. Canals were metrically assessed for changes （surface area, changes in curvature and transportation） during canal preparation by using software SimPlant; instrument failures were also recorded. Mean total widths and outer and inner width measurements were determined on each central canal path and differences were statistically analyzed. The results showed that all instruments maintained the original canal curvature well with no significant differences between the different files （P=0.226）. During preparation there was failure of only one file （the protaper group）. In conclusion, under the conditions of this study, all instruments maintained the original canal curvature well and were safe to use. Areas of uninstrumented root canal wall were left in all regions using the various systems.

Simulation of long-distance pipeline transportation properties of whole-tailings paste with high sliming

Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproperties of high sliming paste from a copper mine in China.Based on the rheological properties test,the pressure and velocity of pipeline and elbow are simulated by CFD under different mass concentrations and different stowing capacities.The results show that the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with high sliming and pumping agent at the same mass concentration,and the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with low sliming while without pumping agent.It is very important to add pumping agent to whole-tailings paste with high sliming,and the resistance changes with mass concentration and stowing capacity at the same cement-to-sand ratio of1:5and tailings-to-waste ratio of6:1.However,the change is just limited,that is to say,the paste transportation system is of good stability.Furthermore,at the elbow,the maximum pressure and velocity transfer to the outside of the pipe from the inside.However,lubricating layer is formed at the pipe wall because of high content of fine particles in whole-tailings paste,which will protect the elbow from abrasion.CFD provides an intuitive and accurate basis for pipeline transportation study,and would have a wider application space in the study of paste rheological properties and resistance reduction methods.

Fracture resistance and pattern of the upper premolars with obturated canals and restored endodontic occlusal access cavities

We studied whether obturing canals and restoring endodontic occlusal access cavities on upper premolars could provide acceptable resistance and pattern to fracture.Eighteen upper premolars were divided equally into 3 groups.Group 1 consisted of intact controls;group 2 had access cavities and root canal preparations;group 3 as in group 2 but obturated with gutta-percha and AH26,and the access cavity restored with glass ionomer and composite.Specimens were submitted to compressive strength testing using the Hounsfield Universal H50KM testing machine with a load cell of 2000 Newtons and a crosshead speed set at 1.0 mm/min until fracture.The results from the compressive strength tests showed that intact controls （1105.83±90.93 MPa） and restored premolars （936.67±44.67 MPa） were significantly different from premolars with unrestored access cavities 568.33±105.49 MPa.There was no significant difference between intact controls and restored premolars.The predominant fracture pattern for intact teeth was an oblique fracture.For premolars that had endodontic access cavities,restored or unrestored,the most common fracture pattern was a vertical fracture.The restoration of occlusal access cavities with glass ionomer and composite provided fracture resistance close to that of intact controls,but when restored teeth fractured,they were predominantly non-restorable.

Endodontic management of the maxillary first molars with two root canals: A case report and review of the literature

BACKGROUND The complex anatomy of the maxillary first molars has always been a major challenge for complete root canal treatment in endodontic therapy. Here, we present two cases of maxillary first molars, each with only two root canals, which have been rarely reported. We also perform a literature review of maxillary first molar anatomy.CASE SUMMARY The two patients were referred to the hospital after 1) finding a cavity in their tooth with a color change and, 2) a toothache during mastication, respectively.Both of these cases were diagnosed as apical periodontitis by X-ray imaging and cone beam computed tomography(CBCT). Non-surgical endodontic therapy was performed with the assistance of a dental operating microscope(DOM). CBCT showed rare but accurate images of both patients, each with two root canals and two roots in their maxillary first molars. Both roots were located in the buccal in the palatal direction, and each root had only one clear root canal. In addition,each maxillary first molar in both patients was symmetrical to that on the opposing side with only two separate root canals. Non-surgical endodontic therapy was performed with the assistance of a DOM. Finally, the teeth were restored using composite resin and the patients were satisfied with the results.CONCLUSION Making full use of CBCT and DOM would contribute to helping dentists make correct diagnoses and successfully treat teeth with rare root canal morphologies.

Three-dimensional inlay-guided endodontics applied in variant root canals: A case report and review of literature

BACKGROUND Root canal retreatment is common after root canal therapy in clinical situations.Especially,completing the retreatment of variant root canals can be challenging.This is particularly true for the molars located at the end of the dental arch.However,advancements in digital dental diagnosis and treatment techniques can solve these problems.Here,we describe a case of a maxillary second molar with a variant distobuccal root canal treated via a novel“inlay-guided endodontics”technique based on improved computer-generated programs.CASE SUMMARY A 63-year-old man complained of a defect in the maxillary left second molar.The tooth,diagnosed with post-treatment endodontic disease,was initially treated by conventional methods,which were ineffective.Our“inlay-guided endodontics”technique was subsequently adopted,with the establishment of a precise integrated three-dimensional(3D)plate model of cone-beam computed tomography data and a digital impression of the dentition.An optimal root canal approach was generated for the“virtual file”in the 3D model.The plate data were imported into a 3D printer and printed.With the help of the guide plate,the file was accurately placed into the cervical third of the distal root canal.The root canal and prosthodontic treatments successfully proceeded subsequently.CONCLUSION Our newly developed inlay guide plates may facilitate individualized and minimally invasive root canal treatment.

Centrifugal and field studies on water infiltration characteristics below canals under wetting-drying-freezing-thawing cycles

Seepage is one of the main causes for the deformation and instability of canal slopes in Xinjiang,China.In this study,centrifugal model tests under wetting-drying(WD)and wetting-drying-freezing-thawing(WDFT)cycles were performed to investigate the water infiltration characteristics below a canal.The results show that the shallow soil of the canal models was fully saturated in the wetting process.Compared with the canal model under the WD cycles,the canal model under the WDFT cycles had larger saturated areas and a higher degree of saturation below the canal top after each cycle,indicating that the freezing-thawing(FT)process in the WDFT cycles promoted the water infiltration behavior below the canal slope.The cracks on the surface of the canal model under the cyclic action of WDFT developed further and had a higher connectivity,which provided the conditions for slope instability from a transverse tensile crack running through the canal top.On this basis,a field test was conducted to understand the water infiltration distribution below a typical canal in Xinjiang,China,which also verified the accuracy of the centrifugal results.This study provides a preliminary basis for the maintenance and seepage treatment of canals in Xinjiang,China.

Water hammer protection for diversion systems in front of pumps in long-distance water supply projects

For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.

Calcium Hydroxide Removal in Curved Root Canals with Apical Transportation In Vitro

Calcium hydroxide（CH） is applied to improve disinfection of root canals in most root canal retreatment. This study aimed to analyze the CH removal efficacy using 7 different root preparing files（K file, pre-curved K file, EndoActivator, Ultrasonic file, pre-curved ultrasonic file, F file and needle irrigation alone） with apical transportation. Standardized models of curved canal with such apical transportation or not were set up before applying CH to root canal for 7 days. Seven techniques described above were used for its removal. Then the roots were disassembled and digital photos were taken. The ratio of residual CH in the overall canal surface was calculated using the image analyzer image pro plus 6.0. The data were analyzed using one-way ANOVA with post hoc Tukey test. Results revealed that CH was effectively removed（P〈0.05） by using all 6 mechanical methods except irrigation alone. In curved root canals with apical transportation, EndoActivator, pre-curved ultrasonic file and F file were found to be more effective in removing CH than the other four file（P〈0.001）, while there was no significant difference among EndoActivator, pre-curved ultrasonic file and F file groups（P〉0.05）. The percentage of residual CH in the canal with apical transportation was higher than that in the canal without apical transportation（P〈0.05）. In conclusion, CH can be hardly removed completely. Canal with apical transportation will result in insufficient CH removal. EndoActivator, pre-curved ultrasonic file and F file are more effective in the curved root canal with apical transportation.

Study on the Technology of Supplying Water Safely by Long-Distance Pipeline

The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status.

Study on the long-distance target apperception techniques for underwater vehicles

The limited physical size for autonomous underwater vehicles （AUV） or unmanned underwater vehicles （UUV） makes it difficult to acquire enough space gain for localizing long-distance targets. A new technique about long-distance target apperception with passive synthetic aperture array for underwater vehicles is presented. First, a synthetic aperture-processing algorithm based on the FFT transform in the beam space （BSSAP） is introduced. Then, the study on the flank array passive long-distance apperception techniques in the frequency scope of 11-18 kHz is implemented from the view of improving array gains, detection probability and augmenting detected range under a certain sea environment. The results show that the BSSAP algorithm can extend the aperture effectively and improve detection probability. Because of the augment of the transmission loss, the detected range has the trend of decline with the increase of frequency under the same target source level. The synthesized array could improve the space gain by nearly 7 dB and SNR is increased by about 5 dB. The detected range is enhanced to nearly 2 km under the condition of 108-118 dB of the target source level for AUV system in measurement interval of nearly 1 s.

Long-distance propagation of pseudo-partially coherent Gaussian Schell-model beams in atmospheric turbulence

Propagation properties of spatially pseudo-partially coherent Gaussian Schell-model beams through the atmo- spheric turbulence over a long-distance uplink path are studied by numerical simulation. A linear coordination trans- formation is introduced to overcome the window effect and the loss-of-resolution problem. The beam spreading, beam wandering, and intensity scintillation as functions of turbulence strength, source correlation length, and change fre- quency of random phase that models the partial coherence of the source are analyzed. It is found that the beam spreading and the intensity scintillation of the partially coherent beam are less affected by the turbulence than those of the coherent one, but it suffers from a more severe diffractive effect, and the change frequency of random phase has no evident influence on it. The beam wandering is insensitive to the variation of source correlation length, and decreases firstly then goes to a fixed value as the change frequency increases.

A Root Canal Therapy on the Maxillary First Molar Tooth with Five Canals: A Case Report

Maxillary first molar usually exhibits a radicular anatomy of three roots and three or four canals. However, different anatomic variations like extra number of roots and canals are possible. For a successful treatment, clinicians must have well equipment and a thorough knowledge of the external and internal anatomy of teeth and its variation. Using of CBCT may help to locate extra canals by giving a chance to clinicians to see the root canal anatomy in 3-D view. The aim of this study was to present a case report about a diagnosis and treatment in maxillary first molar with three roots and five canals. It is concluded that the diagnosing and the treatment of unusual cases are key factors for successful endodontic treatment of these teeth.

Non-surgical management of dens invaginatus type IIIB in maxillary lateral incisor with three root canals and 6-year follow-up:A case report and review of literature

BACKGROUND The presence of dens invaginatus(DI)complicates treatment of any tooth,from diagnosis to access cavity and biomechanical preparation and obturation.Reports of successful non-surgical management of DI type IIIB in maxillary lateral incisor are rare.Here,we report such a case,with three root canals and a long follow-up.CASE SUMMARY A 13-year-old female patient presented with mild pain in the maxillary right lateral incisor(#7)for 10-15 d.On examination,the tooth was slightly rotated,with slight tenderness on percussion and grade I mobility but with no caries,pockets or restorations and non-vital pulp(via vitality tests).Radiographic examination revealed unusual configuration of the tooth’s root canals,with an enamel-lined invagination extending to the apex,suggesting the possibility of DI Oehler’s type IIIB and a periapical radiolucency.Widening the access cavity lingually revealed one distinct buccal orifice and two distinct palatal orifices;under higher magnification of a dental operating microscope(DOM),the mesio palatal and disto-palatal orifices were observed as connected by a C-shaped groove.The root canals were prepared with hand K-files following a step-back technique,and obturated using a combination technique of lateral condensation and vertical compaction.At the 6-year follow-up,the patient was asymptomatic,and the periapical radiography displayed significant healing around the apical end of the root.CONCLUSION Proper knowledge of unusual root canal anatomy is required in treating DI.Conventional methods of root canal treatment can successfully resolve such complex cases,facilitated by DOM and cone-beam computed tomography.

Successful individualized endodontic treatment of severely curved root canals in a mandibular second molar:A case report

BACKGROUND The incidence rate of severely curved root canals in mandibular molars is low,and the root canal treatment of mandibular molars with this aberrant canal anatomy may be technically challenging.CASE SUMMARY A 26-year-old Chinese female patient presented with intermittent and occlusal pain in the left mandibular second molar.The patient had undergone filling restoration for caries before endodontic consultation.With the aid of cone beam computed tomography(CBCT),a large periapical radiolucency was observed,and curved root canals in a mandibular second molar were confirmed,depicting a severe and curved distolingual root.Nonsurgical treatments,including novel individualized preparation skills and techniques and the use of bioceramic materials as an apical barrier,were performed,and complete healing of the periapical lesion and a satisfactory effect were achieved.CONCLUSION A case of severely curved root canals in a mandibular second molar was successfully treated and are reported herein.The complex anatomy of the tooth and the postoperative effect were also evaluated via the three-dimensional reconstruction of CBCT images,which accurately identified the aberrant canal morphology.New devices and biomaterial applications combined with novel synthesis techniques can increase the success rate of intractable endodontic treatment.

Improvement of heat-tolerance by training I. Evaluation of effects of long-distance running and march training on heat-tolerance

in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6～35.6℃) and 10 km running (WBGT 25.0～31.1℃) were performed in laboratory and field under hot climate.Ten to twelve times (days) of training were carried out