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Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species
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作者 Mengmeng Fu Zhen Xu +7 位作者 Huaying Ma Yifan Hao Ji Tian Yi Wang Xinzhong Zhang Xuefeng Xu Zhenhai Han Ting Wu 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第1期25-37,共13页
Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechani... Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs. 展开更多
关键词 Grafting combination long-distance mobile mRNA TLS RNA binding proteins
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Securing offshore resources development:A mathematical investigation into gas leakage in long-distance flexible pipes
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作者 Xiang-An Lu Liang-Liang Jiang +1 位作者 Jian-Sheng Zhang Min-Gui Wang 《Petroleum Science》 SCIE EI CAS CSCD 2024年第4期2734-2744,共11页
Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexib... Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexible pipes and further diffuse into the annular space,incurring annular pressure build-up and/or production of acidic environment,which poses serious challenges to the structure and integrity of the flexible pipes.Gas permeation in pipes is a complex phenomenon governed by various factors such as internal pressure and temperature,annular structure,external temperature.In a long-distance gas flexible pipe,moreover,gas permeation exhibits non-uniform features,and the gas permeated into the annular space flows along the metal gap.To assess the complex gas transport behavior in long-distance gas flexible pipes,a mathematical model is established in this paper considering the multiphase flow phenomena inside the flexible pipes,the diffusion of gas in the inner liner,and the gas seepage in the annular space under varying permeable properties of the annulus.In addition,the effect of a variable temperature is accounted.A numerical calculation method is accordingly constructed to solve the coupling mathematical equations.The annular permeability was shown to significantly influence the distribution of annular pressure.As permeability increases,the annular pressure tends to become more uniform,and the annular pressure at the wellhead rises more rapidly.After annular pressure relief followed by shut-in,the pressure increase follows a convex function.By simulating the pressure recovery pattern after pressure relief and comparing it with test results,we deduce that the annular permeability lies between 123 and 512 m D.The results help shed light upon assessing the annular pressure in long distance gas flexible pipes and thus ensure the security of gas transport in the emerging development of offshore resources. 展开更多
关键词 Offshore resources development Transport security long-distance flexible pipes Gas leakage Heat and mass transfer model Finite difference calculation
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Water hammer protection for diversion systems in front of pumps in long-distance water supply projects 被引量:4
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作者 Lin Shi Jian Zhang +3 位作者 Xiao-dong Yu Sheng Chen Wen-long Zhao Xu-yun Chen 《Water Science and Engineering》 EI CAS CSCD 2023年第2期211-218,共8页
For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention.... For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up. 展开更多
关键词 long-distance diversion pipeline Water supply Water hammer Overflow surge tank Regulating valve
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Simulation of long-distance pipeline transportation properties of whole-tailings paste with high sliming 被引量:18
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作者 WU Ai-xiang RUAN Zhu-en +4 位作者 WANG Yi-ming YIN Sheng-hua WANG Shao-yong WANG Yong WANG Jian-dong 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第1期141-150,共10页
Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproper... Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproperties of high sliming paste from a copper mine in China.Based on the rheological properties test,the pressure and velocity of pipeline and elbow are simulated by CFD under different mass concentrations and different stowing capacities.The results show that the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with high sliming and pumping agent at the same mass concentration,and the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with low sliming while without pumping agent.It is very important to add pumping agent to whole-tailings paste with high sliming,and the resistance changes with mass concentration and stowing capacity at the same cement-to-sand ratio of1:5and tailings-to-waste ratio of6:1.However,the change is just limited,that is to say,the paste transportation system is of good stability.Furthermore,at the elbow,the maximum pressure and velocity transfer to the outside of the pipe from the inside.However,lubricating layer is formed at the pipe wall because of high content of fine particles in whole-tailings paste,which will protect the elbow from abrasion.CFD provides an intuitive and accurate basis for pipeline transportation study,and would have a wider application space in the study of paste rheological properties and resistance reduction methods. 展开更多
关键词 whole-tailings paste long-distance transportation computational fluid dynamics
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Study on the Technology of Supplying Water Safely by Long-Distance Pipeline 被引量:3
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作者 CHEN Yanbo YU Taipin +1 位作者 LIU Junhua ZHAO Hongbin 《Journal of Northeast Agricultural University(English Edition)》 CAS 2008年第3期80-85,共6页
The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, ... The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status. 展开更多
关键词 long-distance water transmission pipeline water supply security information management water hammer MODELING
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Experimental,Numerical,and Analytical Studies on the Bending of Mechanically Lined Pipe 被引量:1
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作者 WEI Wen-bin YUAN Lin +1 位作者 ZHOU Jia-sheng LIU Zheng 《China Ocean Engineering》 SCIE EI CSCD 2024年第2期221-232,共12页
Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which cau... Mechanically lined pipe(MLP)is often used for offshore oil and gas transport because of its low cost and corrosion resistance.During installation and operation,the pipe may undergo severe bending deformation,which causes the liner to separate from the outer pipe and buckles,affecting the stability of the whole line.In this paper,the buckling response of MLP subjected to bending is investigated to clarify its bending characteristics by employing both experiments,numerical simulation,as theoretical methods.Two types of MLPs were manufactured with GB 45 carbon steel(SLP)and Al 6061(ALP)used as the outer pipe material,respectively.The hydraulic expansion and bending experiments of small-scale MLPs are conducted.In addition to the ovalized shape of the cross-section for the SLP specimens,the copper liner was found to wrinkle on the compressive side.In contrast,the liner of ALP remains intact without developing any wrinkling and collapse mode.In addition,a dedicated numerical framework and theoretical models were also established.It was found both the manufacturing and bending responses of the MLP can be well reproduced,and the predicted maximum moment and critical curvatures are in good agreement with the experimental results. 展开更多
关键词 lined pipe BENDING nonlinear ring theory BUCKLING PLASTICITY
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Criterions and Measures of Route Selection of Shallowly Embedded Long-Distance Oil and Gas Pipeline in Mountain Areas 被引量:1
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作者 WANG Chenghua MA Qingwen +2 位作者 KONG Jiming CHEN Zefu LI Xiuzhen 《Wuhan University Journal of Natural Sciences》 EI CAS 2006年第4期787-792,共6页
According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The fo... According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows: the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment. 展开更多
关键词 shallowly embedded long-distance oil and gas pipeline criterions and measures of route selection avoiding geological hazards
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Canine Myofascial Kinetic Lines: A Descriptive Dissection Study Including Related Function and Locomotion and Comparison of the Human and Equine Myofascial Kinetic Lines
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作者 Vibeke S. Elbrønd 《Open Journal of Veterinary Medicine》 CAS 2024年第9期229-256,共28页
Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnec... Aim: This dissection study was conducted to verify if the Myofascial kinetic lines, outlined in detail in humans and recently documented in horses, were present in dogs. These dynamic lines present rows of interconnected muscles, myofascia and other fascia structures, which influence the biomechanics of the spine and limbs. Methods: Forty-two dogs of different breeds and genders were dissected, imaged, and videoed. Results: Similar kinetic lines were verified in the dog, as described in humans and horses, and additionally, three new branches of the lines were discovered. The kinetic lines described were three superficial lines: Dorsal, Ventral, and Lateral, which all started in the hindlimb and ended in the temporal and occipital regions. These lines act respectively in spinal extension, flexion, and lateral flexion. Three profound lines, which started in the tail and ended in the head. The Deep Dorsal Line followed the transversospinal myofascia. The Deep Ventral Line showed an additional start deep in the medial hind limb, continued in the hypaxial myofascia, and enveloped all the viscera. Also, the Deep Lateral Line started in the hindlimb but parted along the trunk in the deep lateral myofascial structures. Two helical lines crossed the midline two or three times and served to rotate the spine. The Functional Line established a sling from the axilla to the contralateral stifle and presented a new ipsilateral branch. The Spiral Line connected the head and the ipsilateral tarsus and additionally presented a new straight branch. The four front limb lines describe their motion: the Front Limb Protraction and Retraction, Adduction, and Abduction Lines. Conclusion: The canine lines mirrored the equine and human lines with exceptions due to differences in anatomy, foot posture, lumbosacral flexibility, and their biomechanical constitution as predator versus prey animals. Additionally, three new canine branches were verified and described. 展开更多
关键词 Myofascial Kinetic lines Canis Familiaris Superficial lines Deep lines Locomotive Connections Viscero-Somatic Connections
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Flood Risk Mapping of the Benin Municipalities at the Intersection of the Coastal Sedimentary Zone and the Crystalline Surface
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作者 Éric Alain Mahugnon Tchibozo Apollinaire Cyriaque Agbon +1 位作者 Azize Ognondoun Bidossessi Roméo David Houessinon 《Journal of Geographic Information System》 2024年第5期321-342,共22页
Climate change and population growth have led to the increase and/or intensification of flooding becoming a major issue. The objective of this study is to visualize flooding risk of municipalities at the intersection ... Climate change and population growth have led to the increase and/or intensification of flooding becoming a major issue. The objective of this study is to visualize flooding risk of municipalities at the intersection of the coastal sedimentary zone and the crystalline surface. The methodology adopted is based on geomatic approach, which involves documentary research, processing and assisted classification using remote sensing images and multi-criteria analysis of the Geographic Information System (GIS). Flooding risk is very high at 8.85% in Djidja, Toffo, Zè and Bonou municipalities. In other municipalities such as Agbangnizoun, Abomey, Bohicon, Za-Kpota and Cove, it is high of 46.85%. To the Southeast of the study area, it is located on the eastern and western banks of Oueme Valley. The medium risk represents 26.35% and is located in the municipalities of Ouinhi and Adjohoun. The other municipalities have a low rate of 17.95%. Risk modeling has made it possible to access the various levels of rising water that can cause flooding. Land-use planning decisions can be influenced by the results of this study. 展开更多
关键词 Geomatic Flood Risk Contact line Municipalities BENIN
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Toward a Direct Measurement of the Cosmic Acceleration:The Pilot Observation of HI 21 cm Absorption Line at FAST
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作者 Jiangang Kang Chang-Zhi Lu +1 位作者 Tong-Jie Zhang Ming Zhu 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第7期47-55,共9页
This study presents results on detecting neutral atomic hydrogen(H I)21 cm absorption in the spectrum of PKS PKS1413+13 at redshift z=0.24670041.The observation was conducted by FAST,with a spectral resolution of10 Hz... This study presents results on detecting neutral atomic hydrogen(H I)21 cm absorption in the spectrum of PKS PKS1413+13 at redshift z=0.24670041.The observation was conducted by FAST,with a spectral resolution of10 Hz,using 10 minutes of observing time.The global spectral profile is examined by modeling the absorption line using a single Gaussian function with a resolution of 10 kHz within a 2 MHz bandwidth.The goal is to determine the rate of the latest cosmic acceleration by directly measuring the redshift evolution of the H I 21 cm absorption line with Hubble flow toward a common background quasar over a decade or longer time span.This will serve as a detectable signal generated by the accelerated expansion of the Universe at redshift z<1,referred to as redshift drift z(5)or the SL effect.The measured H I gas column density in this DLA system is approximately equivalent to the initial observation value,considering uncertainties of the spin temperature of a spiral host galaxy.The high signal-to-noise ratio of 57,obtained at a 10 kHz resolution,strongly supports the feasibility of using the H I 21 cm absorption line in DLA systems to accurately measure the redshift drift rate at a precision level of around 10~(-10)per decade. 展开更多
关键词 (cosmology)cosmological parameters-cosmology observations-cosmology theory-(cosmology)dark energy-radio lines ISM-radio lines galaxies-ISM clouds
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Derivative Spectroscopy and its Application at Detecting the Weak Emission/Absorption Lines
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作者 Lihuan Yu Jiangdan Li +4 位作者 Jinliang Wang Jiajia Li Jiao Li Qiang Xi Zhanwen Han 《Research in Astronomy and Astrophysics》 SCIE CAS CSCD 2024年第3期205-223,共19页
The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened ... The development of spectroscopic survey telescopes like Large Sky Area Multi-Object Fiber Spectroscopic Telescope(LAMOST),Apache Point Observatory Galactic Evolution Experiment and Sloan Digital Sky Survey has opened up unprecedented opportunities for stellar classification.Specific types of stars,such as early-type emission-line stars and those with stellar winds,can be distinguished by the profiles of their spectral lines.In this paper,we introduce a method based on derivative spectroscopy(DS)designed to detect signals within complex backgrounds and provide a preliminary estimation of curve profiles.This method exhibits a unique advantage in identifying weak signals and unusual spectral line profiles when compared to other popular line detection methods.We validated our approach using synthesis spectra,demonstrating that DS can detect emission signals three times fainter than Gaussian fitting.Furthermore,we applied our method to 579,680 co-added spectra from LAMOST Medium-Resolution Spectroscopic Survey,identifying 16,629 spectra with emission peaks around the Hαline from 10,963 stars.These spectra were classified into three distinct morphological groups,resulting in nine subclasses as follows.(1)Emission peak above the pseudo-continuum line(single peak,double peaks,emission peak situated within an absorption line,P Cygni profile,Inverse P Cygni profile);(2)Emission peak below the pseudo-continuum line(sharp emission peak,double absorption peaks,emission peak shifted to one side of the absorption line);(3)Emission peak between the pseudo-continuum line. 展开更多
关键词 line identification-line profiles-methods data analysis-techniques spectroscopic-techniques radial velocities
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Petrology of Spinel-Lherzolite Xenoliths from Mazéléand Others Northen Xenoliths Localities of Cameroon Volcanic Line: Exchange Reactions and Equilibrium State
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作者 Nguihdama Dagwai Kamgang Pierre +2 位作者 Mbowou Gbambié Isaac Bertrand Chazot Gilles Ngounouno Ismaïla 《Open Journal of Geology》 CAS 2024年第5期629-653,共25页
The alkaline volcanism of the Cameroon Volcanic Line in its northern domain has raised many fresh enclaves of peridotites. The samples selected come from five (05) different localities (Liri, in the plateau of Kapsiki... The alkaline volcanism of the Cameroon Volcanic Line in its northern domain has raised many fresh enclaves of peridotites. The samples selected come from five (05) different localities (Liri, in the plateau of Kapsiki, Mazélé in the NE of Ngaoundéré, Tello and Ganguiré in the SE of Ngaoundéré and Likok, locality located in the west of Ngaoundé). The peridotite enclaves of the above localities show restricted mineralogical variation. Most are four-phase spinel-lherzolites, indicating that this is the main lithology that forms the lithospheric mantle below the shallow zone. No traces of garnet or primary plagioclase were detected, which strongly limits the depth range from which the rock fragments were sampled. The textures and the wide equilibrium temperatures (884˚C - 1115˚C) indicate also entrainment of lherzolite xenoliths from shallow depths within the lithosphere and the presence of mantle diapirism. The exchange reactions and equilibrium state established in this work make it possible to characterize the chemical composition of the upper mantle of each region and test the equilibrium state of the phases between them. Variations of major oxides and incompatible elemental concentrations in clinopyroxene indicate a primary control by partial melting. The absence of typical “metasomatic” minerals, low equilibration temperatures and enriched LREE patterns indicate that the upper mantle below septentrional crust of Cameroun underwent an event of cryptic metasomatic enrichment prior to partial melting. The distinctive chemical features, LREE enrichment, strong U, Ce and Pr, depletion relative to Ba, Nb, La, Pb, and T, fractionation of Zr and Hf and therefore ligh high Zr/Hf ratio, low La/Yb, Nb/La and Ti/Eu are all results of interaction of refractory peridotite residues with carbonatite melts. 展开更多
关键词 XENOLITHS Upper Mantle Northern Region Adamawa Cameroon Volcanic line Exchange Reaction
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Evaluation of red soil-bentonite mixtures for compacted clay liners
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作者 A.S.Devapriya T.Thyagaraj 《Journal of Rock Mechanics and Geotechnical Engineering》 SCIE CSCD 2024年第2期697-710,共14页
Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are u... Compacted clay liners are an integral part of the waste landfills,which are provided to contain the leachate within the landfills and protect the surrounding environment.Generally,locally available natural soils are used for the construction of compacted clay liners if they satisfy the design criteria.However,not all soils in their natural state satisfy all the design criteria for the liner materials.Thus,there is a definite need to modify the locally available natural soils by blending with bentonite to meet the required design criteria for the liners.In view of this,the present study evaluates the suitability of an Indian red soil enhanced with bentonite as a liner material.To achieve this,a series of experiments were carried out using locally available red soil and bentonite.First,the suitability of the red soil was evaluated as a liner material.The experimental results showed that the red soil met all the selection criteria stipulated by the Environmental Protection Agencies(EPAs)for the liners except the hydraulic conductivity criterion.Therefore,the red soil was mixed with bentonite contents of 10%,20%and 30%,and the red soil-bentonite mixtures were evaluated for their suitability for liners in their compacted state.Further,as the liners in the arid and semi-arid regions are subjected to moisture variations due to seasonal moisture fluctuations and other factors,the red soil-bentonite mixtures were subjected to wetdry cycles,and their suitability was evaluated after wet-dry cycles.The experimental results revealed that all the red soil-bentonite mixtures met the stipulated EPA criteria for the liners in the as-compacted state.However,the red soil-bentonite mixtures with 20%and 30%bentonite contents only satisfied the hydraulic conductivity requirement even after wet-dry cycles.The experimental findings were supplemented with the microstructural insights captured through digital camera images,scanning electron microscopy(SEM),and mercury intrusion porosimetry(MIP)studies. 展开更多
关键词 Compacted clay lines Hydraulic conductivity Wet-dry cycles Microstructure
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Establishment and characterization of a canine chondrosarcoma cell line:Mango
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作者 Meilin Wang Xiao Wang +8 位作者 Lixin He Hongbo Gao Wenxuan Li Huili Feng Qingyuan Zhao Wenwen Zhang Chengzong Li Bohan Zhang Changwei Qiu 《Animal Diseases》 CAS 2024年第3期186-197,共12页
In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines ha... In the global progress of bone tumor research,established stable and long-lasting transgenic chondrosarcoma(CSA)cell lines are rare,mainly of murine and human origin,while the establishment of canine CSA cell lines has yet to be reported.This study established a canine CSA cell line to facilitate the basic clinical study of canine CSA.Fifty fve cases of canine osteolytic disease were collected,and more than 10 bone tumor samples from dogs with typical clinical signs were used for primary cell culture.A cell line with stable passaging for more than 100 generations and mouse tumorigenic ability was successfully cultured.According to the clinical characteristics of the dog and the histopathological results of the primary tumor,CSA was diagnosed,and the CSA cell line was designated Mango.Immunohistochemical(IHC)results showed that the immunoreactivity of bone gamma-carboxyglutamate protein(BGLAP),secreted protein acidic and rich in cysteine(SPARC),alkaline phosphatase(ALPL),vimentin(VIM)and S100 were positive.However,the immunoreactivity of pan-cytokeratin(PCK),chromogranin A(CGA),and platelet endothelial cell adhesion molecule-1(CD31)was negative.Immunofuorescence(IF)results showed that the protein expressions in the Mango cell line were consistent with the IHC identifcation of the primary tumor.The Mango cell line’s doubling time was 43.92 h,and the cell formation rate exceeded 20%.There were abnormal chromosome numbers,hetero staining with toluidine blue,and certain calcifcation abilities.It could be passaged stably and continuously without changing the cell morphology and characteristics.In vivo,the cells were successfully injected into the nude mice model with a tumorigenic rate of 100%.The immunophenotype of the xenograft tumor was consistent with that of the primary tumor.Therefore,we efectively established a canine CSA cell line.As a promising cell material,this cell line can be used to construct a tumor-bearing model conducive to the subsequent basic research of canine CSA.Moreover,because of its similarity to human CSA,the animal model of CSA is also indispensable for investigating human CSA. 展开更多
关键词 Canine chondrosarcoma Cell line ESTABLISHMENT CHARACTERIZATION Tumorigenicity
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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines
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作者 Justus Thomas Obiajulu Sievers Anom Bowolaksono R.Tedjo Sasmono 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第5期215-224,共10页
Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,J... Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery. 展开更多
关键词 Zika virus Infectivity REPLICATION Cell lines In vitro
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An Implementation of Multiscale Line Detection and Mathematical Morphology for Efficient and Precise Blood Vessel Segmentation in Fundus Images
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作者 Syed Ayaz Ali Shah Aamir Shahzad +4 位作者 Musaed Alhussein Chuan Meng Goh Khursheed Aurangzeb Tong Boon Tang Muhammad Awais 《Computers, Materials & Continua》 SCIE EI 2024年第5期2565-2583,共19页
Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when deal... Diagnosing various diseases such as glaucoma,age-related macular degeneration,cardiovascular conditions,and diabetic retinopathy involves segmenting retinal blood vessels.The task is particularly challenging when dealing with color fundus images due to issues like non-uniformillumination,low contrast,and variations in vessel appearance,especially in the presence of different pathologies.Furthermore,the speed of the retinal vessel segmentation system is of utmost importance.With the surge of now available big data,the speed of the algorithm becomes increasingly important,carrying almost equivalent weightage to the accuracy of the algorithm.To address these challenges,we present a novel approach for retinal vessel segmentation,leveraging efficient and robust techniques based on multiscale line detection and mathematical morphology.Our algorithm’s performance is evaluated on two publicly available datasets,namely the Digital Retinal Images for Vessel Extraction dataset(DRIVE)and the Structure Analysis of Retina(STARE)dataset.The experimental results demonstrate the effectiveness of our method,withmean accuracy values of 0.9467 forDRIVE and 0.9535 for STARE datasets,aswell as sensitivity values of 0.6952 forDRIVE and 0.6809 for STARE datasets.Notably,our algorithmexhibits competitive performance with state-of-the-art methods.Importantly,it operates at an average speed of 3.73 s per image for DRIVE and 3.75 s for STARE datasets.It is worth noting that these results were achieved using Matlab scripts containing multiple loops.This suggests that the processing time can be further reduced by replacing loops with vectorization.Thus the proposed algorithm can be deployed in real time applications.In summary,our proposed system strikes a fine balance between swift computation and accuracy that is on par with the best available methods in the field. 展开更多
关键词 line detector vessel detection LOCALIZATION mathematical morphology image processing
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ST-LaneNet: Lane Line Detection Method Based on Swin Transformer and LaneNet
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作者 Yufeng Du Rongyun Zhang +3 位作者 Peicheng Shi Linfeng Zhao Bin Zhang Yaming Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2024年第1期130-145,共16页
The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line dete... The advancement of autonomous driving heavily relies on the ability to accurate lane lines detection.As deep learning and computer vision technologies evolve,a variety of deep learning-based methods for lane line detection have been proposed by researchers in the field.However,owing to the simple appearance of lane lines and the lack of distinctive features,it is easy for other objects with similar local appearances to interfere with the process of detecting lane lines.The precision of lane line detection is limited by the unpredictable quantity and diversity of lane lines.To address the aforementioned challenges,we propose a novel deep learning approach for lane line detection.This method leverages the Swin Transformer in conjunction with LaneNet(called ST-LaneNet).The experience results showed that the true positive detection rate can reach 97.53%for easy lanes and 96.83%for difficult lanes(such as scenes with severe occlusion and extreme lighting conditions),which can better accomplish the objective of detecting lane lines.In 1000 detection samples,the average detection accuracy can reach 97.83%,the average inference time per image can reach 17.8 ms,and the average number of frames per second can reach 64.8 Hz.The programming scripts and associated models for this project can be accessed openly at the following GitHub repository:https://github.com/Duane 711/Lane-line-detec tion-ST-LaneNet. 展开更多
关键词 Autonomous driving Lane line detection Deep learning Swin transformer
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On traceable iterated line graph and hamiltonian path index
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作者 NIU Zhao-hong XIONG Li-ming YANG Wei-hua 《Applied Mathematics(A Journal of Chinese Universities)》 SCIE CSCD 2024年第2期239-252,共14页
Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characteri... Xiong and Liu[21]gave a characterization of the graphs G for which the n-iterated line graph L^(n)(G)is hamiltonian,for n≥2.In this paper,we study the existence of a hamiltonian path in L^(n)(G),and give a characterization of G for which L^(n)(G)has a hamiltonian path.As applications,we use this characterization to give several upper bounds on the hamiltonian path index of a graph. 展开更多
关键词 iterated line graph TRACEABLE hamiltonian index hamiltonian path index
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Imaging of human parafoveal area with large field of view in adaptive optics line scanning ophthalmoscope
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作者 Wen Kong Yiwei Chen +1 位作者 Guohua Shi Yi He 《Journal of Innovative Optical Health Sciences》 SCIE EI CSCD 2024年第6期41-51,共11页
The parafoveal area,with its high concentration of photoreceptors andfine retinal capillaries,is crucial for central vision and often exhibits early signs of pathological changes.The current adaptive optics scanning l... The parafoveal area,with its high concentration of photoreceptors andfine retinal capillaries,is crucial for central vision and often exhibits early signs of pathological changes.The current adaptive optics scanning laser ophthalmoscope(AOSLO)provides an excellent tool to acquire accurate and detailed information about the parafoveal area with cellular resolution.However,limited by the scanning speed of two-dimensional scanning,thefield of view(FOV)in the AOSLO system was usually less than or equal to 2,and the stitching for the parafoveal area required dozens of images,which was time-consuming and laborious.Unfortunately,almost half of patients are unable to obtain stitched images because of their poorfixation.To solve this problem,we integrate AO technology with the line-scan imaging method to build an adaptive optics line scanning ophthalmoscope(AOLSO)system with a larger FOV.In the AOLSO,afocal spherical mirrors in pairs are nonplanar arranged and the distance and angle between optical elements are optimized to minimize the aberrations,two cylinder lenses are orthogonally placed before the imaging sensor to stretch the point spread function(PSF)for sufficiently digitizing light energy.Captured human retinal images show the whole parafoveal area with 55FOV,60 Hz frame rate and cellular resolutions.Take advantage of the 5FOV of the AOLSO,only 9 frames of the retina are captured with several minutes to stitch a montage image with an FOV of 99,in which photoreceptor counting is performed within approximately 5eccentricity.The AOLSO system not only provides cellular resolution but also has the capability to capture the parafoveal region in a single frame,which offers great potential for noninvasive studying of the parafoveal area. 展开更多
关键词 Adaptive optics line scanning retinal imaging system parafoveal capillary vasculature.
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Learning Epipolar Line Window Attention for Stereo Image Super-Resolution Reconstruction
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作者 Xue Li Hongying Zhang +1 位作者 Zixun Ye Xiaoru 《Computers, Materials & Continua》 SCIE EI 2024年第2期2847-2864,共18页
Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not... Transformer-based stereo image super-resolution reconstruction(Stereo SR)methods have significantly improved image quality.However,existing methods have deficiencies in paying attention to detailed features and do not consider the offset of pixels along the epipolar lines in complementary views when integrating stereo information.To address these challenges,this paper introduces a novel epipolar line window attention stereo image super-resolution network(EWASSR).For detail feature restoration,we design a feature extractor based on Transformer and convolutional neural network(CNN),which consists of(shifted)window-based self-attention((S)W-MSA)and feature distillation and enhancement blocks(FDEB).This combination effectively solves the problem of global image perception and local feature attention and captures more discriminative high-frequency features of the image.Furthermore,to address the problem of offset of complementary pixels in stereo images,we propose an epipolar line window attention(EWA)mechanism,which divides windows along the epipolar direction to promote efficient matching of shifted pixels,even in pixel smooth areas.More accurate pixel matching can be achieved using adjacent pixels in the window as a reference.Extensive experiments demonstrate that our EWASSR can reconstruct more realistic detailed features.Comparative quantitative results show that in the experimental results of our EWASSR on the Middlebury and Flickr1024 data sets for 2×SR,compared with the recent network,the Peak signal-to-noise ratio(PSNR)increased by 0.37 dB and 0.34 dB,respectively. 展开更多
关键词 Stereo SR epipolar line window attention feature distillation
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