Characteristics of long-distance mobile mRNAs from shoot to root in grafted plant species

Thousands of long-distance mobile mRNAs were identified from different grafting systems,based on high-throughput sequencing technology.Moreover,the long-distance delivery of RNAs was proved to involve multiple mechanisms.Here,we analyzed the homology,motif,and tRNA-like structure(TLS)of long-distance mobile mRNAs identified by RNA-seq as well as the RNA-binding protein(RBP)in nine grafting combinations including Arabidopsis thaliana,Vitis vinifera,Cucumis sativus,Citrullus lanatus,Nicotiana benthamiana,Malus domestica,Pyrus spp.,Glycine max and Phaseolus vulgaris.Although several mRNAs were found to be shared in herbaceous,woody,and related species,the vast majority of long-distance mobile mRNAs were species-specific.Four non-specific movement-related motifs were identified,while the TLS was not necessary for mRNA long distance mobility.In addition,we found that RBPs were conserved among herbaceous and woody plants as well as related species.This paper reports a further in-depth analysis of the endogenous mechanisms by which the species-specific transportable m RNAs were selected by bioinformatics,in order to provide insights for future research on long-distance mobile mRNAs.

Securing offshore resources development:A mathematical investigation into gas leakage in long-distance flexible pipes

Gas flexible pipes are critical multi-layered equipment for offshore oil and gas development.Under high pressure conditions,small molecular components of natural gas dissolve into the polymer inner liner of the flexible pipes and further diffuse into the annular space,incurring annular pressure build-up and/or production of acidic environment,which poses serious challenges to the structure and integrity of the flexible pipes.Gas permeation in pipes is a complex phenomenon governed by various factors such as internal pressure and temperature,annular structure,external temperature.In a long-distance gas flexible pipe,moreover,gas permeation exhibits non-uniform features,and the gas permeated into the annular space flows along the metal gap.To assess the complex gas transport behavior in long-distance gas flexible pipes,a mathematical model is established in this paper considering the multiphase flow phenomena inside the flexible pipes,the diffusion of gas in the inner liner,and the gas seepage in the annular space under varying permeable properties of the annulus.In addition,the effect of a variable temperature is accounted.A numerical calculation method is accordingly constructed to solve the coupling mathematical equations.The annular permeability was shown to significantly influence the distribution of annular pressure.As permeability increases,the annular pressure tends to become more uniform,and the annular pressure at the wellhead rises more rapidly.After annular pressure relief followed by shut-in,the pressure increase follows a convex function.By simulating the pressure recovery pattern after pressure relief and comparing it with test results,we deduce that the annular permeability lies between 123 and 512 m D.The results help shed light upon assessing the annular pressure in long distance gas flexible pipes and thus ensure the security of gas transport in the emerging development of offshore resources.

Water hammer protection for diversion systems in front of pumps in long-distance water supply projects

For a water supply system with long-distance diversion pipelines, in addition to the water hammer problems that occur beyond pumps, the safety of the water diversion pipeline in front of pumps also deserves attention. In this study, a water hammer protection scheme combined with an overflow surge tank and a regulating valve was developed. A mathematical model of the overflow surge tank was developed, and an analytical formula for the height of the overflow surge tank was derived. Furthermore, a practical water supply project was used to evaluate the feasibility of the combined protection scheme and analyze the sensitivity of valve regulation rules. The results showed that the combined protection scheme effectively reduced the height of the surge tank, lessened the difficulties related to construction, and reduced the necessary financial investment for the project. The two-stage closing rule articulated as fast first and then slow could minimize the overflow volume of the surge tank when the power failure occurred, while the two-stage opening rule articulated as slow first and then fast could be more conducive to the safety of the water supply system when the pump started up.

Instability mechanism of mining roadway passing through fault at different angles in kilometre-deep mine and control measures of roof cutting and NPR cables

The angle α between the fault strike and the axial direction of the roadway produces different damage characteristics. In this paper, the research methodology includes theoretical analyses, numerical simulations and field experiments in the context of the Daqiang coal mine located in Shenyang, China. The stability control countermeasure of "pre-splitting cutting roof + NPR anchor cable"(PSCR-NPR) is simultaneously proposed. According to the different deformation characteristics of the roadway, the faults are innovatively classified into three types, with α of type I being 0°-30°, α of type II being 30°-60°, and α of type III being 60°-90°. The full-cycle stress evolution paths during mining roadway traverses across different types of faults are investigated by numerical simulation. Different pinch angles α lead to high stress concentration areas at different locations in the surrounding rock. The non-uniform stress field formed in the shallow surrounding rock is an important reason for the instability of the roadway. The pre-cracked cut top shifted the high stress region to the deep rock mass and formed a low stress region in the shallow rock mass. The high prestressing NPR anchor cable transforms the non-uniform stress field of the shallow surrounding rock into a uniform stress field. PSCR-NPR is applied in the fault-through roadway of Daqiang mine. The low stress area of the surrounding rock was enlarged by 3-7 times, and the cumulative convergence was reduced by 45%-50%. It provides a reference for the stability control of the deep fault-through mining roadway.

Mechanism of principal stress rotation and deformation failure behavior induced by excavation in roadways

The failure modes of rock after roadway excavation are diverse and complex.A comprehensive investigation of the internal stress field and the rotation behavior of the stress axis in roadways is essential for elucidating the mechanism of roadway failure.This study aimed to examine the spatial relationship between roadways and stress fields.The law of stress axis rotation under three-dimensional(3D)stress has been extensively studied.A stress model of roadways in the spatial stress field was established,and the far-field stress state at different spatial positions of the roadways was analyzed.A mechanical model of roadways under a 3D stress state was established using far-field stress solutions as boundary conditions.The distribution of principal stressesσ1,σ2 andσ3 around the roadways and the variation of the stress principal axis were solved.It was found that the stability boundary of the stress principal axis exhibits hysteresis when compared with that of the principal stress magnitudes.A numerical analysis model for spatial roadways was established to validate the distribution of principal stress and the mechanism of principal axis rotation.Research has demonstrated that the stress axis undergoes varying degrees of spatial rotation in different orientations and radial depths.Based on the distribution of principal stress and the rotation law of the stress principal axis,the entire evolution mechanism of the two stress adjustments to form the final failure form after roadway excavation has been revealed.The on-site detection results also corroborate the findings presented in this paper.The results provide a basis for the analysis of the failure mechanism under a 3D stress state.

Sub-Homogeneous Peridynamic Model for Fracture and Failure Analysis of Roadway Surrounding Rock

The surrounding rock of roadways exhibits intricate characteristics of discontinuity and heterogeneity.To address these complexities,this study employs non-local Peridynamics(PD)theory and reconstructs the kernel function to represent accurately the spatial decline of long-range force.Additionally,modifications to the traditional bondbased PD model are made.By considering the micro-structure of coal-rock materials within a uniform discrete model,heterogeneity characterized by bond random pre-breaking is introduced.This approach facilitates the proposal of a novel model capable of handling the random distribution characteristics of material heterogeneity,rendering the PD model suitable for analyzing the deformation and failure of heterogeneous layered coal-rock mass structures.The established numerical model and simulation method,termed the sub-homogeneous PD model,not only incorporates the support effect but also captures accurately the random heterogeneous micro-structure of roadway surrounding rock.The simulation results obtained using this model show good agreement with field measurements from the Fucun coal mine,effectively validating the model’s capability in accurately reproducing the deformation and failure mode of surrounding rock under bolt-supported(anchor cable).The proposed subhomogeneous PD model presents a valuable and effective simulation tool for studying the deformation and failure of roadway surrounding rock in coal mines,offering new insights and potential advancements.

Failure mechanism and safety control technology of a composite strata roadway in deep and soft rock masses:a case study

The construction of coal mines often encounters deep composite soft rock roadways,which is characterized by significant deformation and poor stability.To deeply study the failure mechanism and large deformation challenges of a composite strata roadway in deep and soft rock masses,a numerical model of 3DEC tetrahedral blocks was established based on the method of rock quality designation(RQD).The results showed that original support cannot prevent asymmetric failure and large deformation due to the adverse geological environment and unsuitable support design.According to the failure characteristics,a coupling support of“NPR bolt/cable+mesh+shotcrete+steel pipe”was proposed to control the stability of the surrounding rock.The excellent mechanical properties of large deformation(approximately 400 mm)and high constant resistance force(bolt with 180 k N;cable with 350 k N)were evaluated by the tensile tests.The numerical results showed that the maximum deformation was minimized to 243 mm,and the bearing capacity of the surrounding rock of the roadway was enhanced.The field test results showed that the maximum deformation of the surrounding rock was 210 mm,and the forces of the NPR bolt and cable were stable at approximately 180 k N and 350 k N,respectively.This demonstrated the effectiveness of the coupling support with the NPR bolt and cable,which could be a guiding significance for the safety control of large deformation and failure in deep composite soft rock roadways.

The Impact of Sea Level Rise on Roadway Design and Evacuation Routes in Delaware

As the global temperature continues to increase, the sea level continues to rise at a rapid rate that has never been seen before. This becomes an issue for many facets of life but one of the most impacted is the transportation infrastructure. Many people living in low elevation coastal areas can become trapped by flooding with no way in or out. With Delaware being a coastal state, this would affect a large portion of the population and will have detrimental effects over time if nothing is done to combat sea level rise. The issue with sea level rise in transportation is that once the roads become flooded, they become virtually unusable and detour routes would be needed. If all the roads in a coastal area were to be affected by sea level rise, the options for detours would become limited. This article looks at direct solutions to combat sea level rise and indirect solutions that would specifically help transportation infrastructure and evacuation routes in Delaware. There is not one solution that can fix every problem, so many solutions are laid out to see what is applicable to each affected area. Some solutions include defense structures that would be put close to the coast, raising the elevation of vulnerable roads throughout the state and including pumping stations to drain the water on the surface of the road. With an understanding of all these solutions around the world, the ultimate conclusion came in the form of a six-step plan that Delaware should take in order to best design against sea level rise in these coastal areas.

Bearing mechanism of roof and rib support structure in automatically formed roadway and its support design method

Non-pillar mining technology with automatically formed roadway is a new mining method without coal pillar reservation and roadway excavation.The stability control of automatically formed roadway is the key to the successful application of the new method.In order to realize the stability control of the roadway surrounding rock,the mechanical model of the roof and rib support structure is established,and the influence mechanism of the automatically formed roadway parameters on the compound force is revealed.On this basis,the roof and rib support structure technology of confined lightweight concrete is proposed,and its mechanical tests under different eccentricity are carried out.The results show that the bearing capacity of confined lightweight concrete specimens is basically the same as that of ordinary confined concrete specimens.The bearing capacity of confined lightweight concrete specimens under different eccentricities is 1.95 times higher than those of U-shaped steel specimens.By comparing the test results with the theoretical calculated results of the confined concrete,the calculation method of the bearing capacity for the confined lightweight concrete structure is selected.The design method of confined lightweight concrete support structure is established,and is successfully applied in the extra-large mine,Ningtiaota Coal Mine,China.

Theory,technology and application of grouted bolting in soft rock roadways of deep coal mines

The grouted bolt,combining rock bolting with grouting techniques,provides an effective solution for controlling the surrounding rock in deep soft rock and fractured roadways.It has been extensively applied in numerous deep mining areas characterized by soft rock roadways,where it has demonstrated remarkable control results.This article systematically explores the evolution of grouted bolting,covering its theoretical foundations,design methods,materials,construction processes,monitoring measures,and methods for assessing its effectiveness.The overview encompassed several key elements,delving into anchoring theory and grouting reinforcement theory.The new principle of high pretensioned high-pressure splitting grouted bolting collaborative active control is introduced.A fresh method for dynamic information design is also highlighted.The discussion touches on both conventional grouting rock bolts and cable bolts,as well as innovative grouted rock bolts and cables characterized by their high pretension,strength,and sealing hole pressure.An examination of the merits and demerits of standard inorganic and organic grouting materials versus the new inorganic–organic composite materials,including their specific application conditions,was conducted.Additionally,the article presents various methods and instruments to assess the support effect of grouting rock bolts,cable bolts,and grouting reinforcement.Furthermore,it provides a foundation for understanding the factors influencing decisions on grouted bolting timing,the sequence of grouting,the pressure applied,the volume of grout used,and the strategic arrangement of grouted rock bolts and cable bolts.The application of the high pretensioned high-pressure splitting grouted bolting collaborative control technology in a typical kilometer-deep soft rock mine in China—the soft coal seam and soft rock roadway in the Kouzidong coal mine,Huainan coal mining area,was introduced.Finally,the existing problems in grouted bolting control technology for deep soft rock roadways are analyzed,and the future development trend of grouted bolting control technology is anticipated.

Surrounding Rock Control Technology of Strong Dynamic Pressure Roadway in Hudi Coal Industry

Aiming at the problems of large deformation and difficult maintenance of deep soft rock roadway under the influence of high ground stress and strong dynamic pressure, taking the surrounding rock control of 1105 lane in Hudi Coal Industry as an example, the deformation characteristics and surrounding rock control measures of deep soft rock roadway are analyzed and discussed by means of geological data analysis, roadway deformation monitoring, rock crack drilling and field test. The results show that the main causes of roadway deformation are high ground stress, synclinal tectonic stress, advance mining stress, roadway penetration and surrounding rock fissure development. Based on the deformation characteristics and mechanism of lane 1105, the supporting countermeasures of “roof synergic support, layered grouting, anchor cable beam support, closed hardening of roadway surface” are proposed, which can provide reference for the control of deep roadway surrounding rock under similar conditions.

Simulation of long-distance pipeline transportation properties of whole-tailings paste with high sliming

Based on Bingham rheological model,a three-dimensional numerical simulation model for long-distance pipeline transportation is established by computational fluid dynamics(CFD)to study the pipeline transportationproperties of high sliming paste from a copper mine in China.Based on the rheological properties test,the pressure and velocity of pipeline and elbow are simulated by CFD under different mass concentrations and different stowing capacities.The results show that the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with high sliming and pumping agent at the same mass concentration,and the pipeline resistance of whole-tailings paste with high sliming while without pumping agent is much higher than that with low sliming while without pumping agent.It is very important to add pumping agent to whole-tailings paste with high sliming,and the resistance changes with mass concentration and stowing capacity at the same cement-to-sand ratio of1:5and tailings-to-waste ratio of6:1.However,the change is just limited,that is to say,the paste transportation system is of good stability.Furthermore,at the elbow,the maximum pressure and velocity transfer to the outside of the pipe from the inside.However,lubricating layer is formed at the pipe wall because of high content of fine particles in whole-tailings paste,which will protect the elbow from abrasion.CFD provides an intuitive and accurate basis for pipeline transportation study,and would have a wider application space in the study of paste rheological properties and resistance reduction methods.

Study on the Technology of Supplying Water Safely by Long-Distance Pipeline

The extensively built long-distance water transmission pipelines have become the main water sources for urban areas. To ensure the reliability and safety of the water supply, from the viewpoint of overall management, it would be necessary to establish a system of information management for the pipeline. The monitoring, calculating and analyzing functions of the system serve to give controlling instructions and safe operating rules to the automatic equipment and technician, making sure the resistance coefficient distribution along the pipeline is reasonable; the hydraulic state transition is smooth when operating conditions change or water supply accidents occur, avoiding the damage of water hammer. This paper covered the composition structures of the information management system of long-distance water transmission pipelines and the functions of the subsystems, and finally elaborated on the approaches and steps of building a mathematics model for the analysis of dynamic hydraulic status.

Study on the long-distance target apperception techniques for underwater vehicles

The limited physical size for autonomous underwater vehicles （AUV） or unmanned underwater vehicles （UUV） makes it difficult to acquire enough space gain for localizing long-distance targets. A new technique about long-distance target apperception with passive synthetic aperture array for underwater vehicles is presented. First, a synthetic aperture-processing algorithm based on the FFT transform in the beam space （BSSAP） is introduced. Then, the study on the flank array passive long-distance apperception techniques in the frequency scope of 11-18 kHz is implemented from the view of improving array gains, detection probability and augmenting detected range under a certain sea environment. The results show that the BSSAP algorithm can extend the aperture effectively and improve detection probability. Because of the augment of the transmission loss, the detected range has the trend of decline with the increase of frequency under the same target source level. The synthesized array could improve the space gain by nearly 7 dB and SNR is increased by about 5 dB. The detected range is enhanced to nearly 2 km under the condition of 108-118 dB of the target source level for AUV system in measurement interval of nearly 1 s.

Long-distance propagation of pseudo-partially coherent Gaussian Schell-model beams in atmospheric turbulence

Propagation properties of spatially pseudo-partially coherent Gaussian Schell-model beams through the atmo- spheric turbulence over a long-distance uplink path are studied by numerical simulation. A linear coordination trans- formation is introduced to overcome the window effect and the loss-of-resolution problem. The beam spreading, beam wandering, and intensity scintillation as functions of turbulence strength, source correlation length, and change fre- quency of random phase that models the partial coherence of the source are analyzed. It is found that the beam spreading and the intensity scintillation of the partially coherent beam are less affected by the turbulence than those of the coherent one, but it suffers from a more severe diffractive effect, and the change frequency of random phase has no evident influence on it. The beam wandering is insensitive to the variation of source correlation length, and decreases firstly then goes to a fixed value as the change frequency increases.

Improvement of heat-tolerance by training I. Evaluation of effects of long-distance running and march training on heat-tolerance

in order to verify the heat-tolerance effect, two trainings, 90 min marching with load (WBGT 24. 6～35.6℃) and 10 km running (WBGT 25.0～31.1℃) were performed in laboratory and field under hot climate.Ten to twelve times (days) of training were carried out

Influencing Factors of Seed Long-distance Dispersal on a Fragmented Forest Landscape on Changbai Mountains,China

Seed long-distance dispersal(LDD) events are typically rare, but are important in the population processes that determine large-scale forest changes and the persistence of species in fragmented landscapes. However, previous studies focused on species dispersed via animal-mediated LDD, and ignored those dispersed by wind. The aim of this study was to assess the effects of canopy openness, edge, seed source, and patch tree density on the LDD of seeds by wind in forest. We collected birch seeds, a typical wind-dispersed species, throughout a larch plantation. We then assessed the relationship between birch LDD and each factor that may influence LDD of seeds by wind including distance to edge, canopy openness size, distance to mature forest, and the tree density of the larch plantation. We used univariate linear regression analysis to assess the influence of those factors on birch LDD, and partial correlations to calculate the contribution of each factor to LDD. The results showed that both canopy openness and edge had significant influences on birch LDD. Specifically, a negative relationship was observed between distance to edge and birch LDD, whereas there was a positive correlation between canopy openness size and LDD. In contrast, the distance to the mature forest was not correlated with birch LDD. Our results suggest that patch tree density could potently affect the probability of LDD by wind vectors, which provides novel and revealing insights regarding the effect of fragmentation on wind dynamics. The data also provide compelling evidence for the previously undocumented effect of habitat fragmentation on wind-dispersed organisms. As such, these observations will facilitate reasonable conservation planning, which requires a detailed understanding of the mechanisms by which patch properties hamper the delivery of seeds of wind-dispersed plants to fragmented areas.

Criterions and Measures of Route Selection of Shallowly Embedded Long-Distance Oil and Gas Pipeline in Mountain Areas

According to the engineering investigation of long-distance oil and gas pipelines, the criterions and measures of route selection are drawn as follows： the flat landform is the first choice in route alignment. The foot of mountain is the first choice when the route passes by the valley. The route should pass by but the shady and deposited slope and not in sunny and erosive slope as possible as it can. The pipeline should be vertical to contour climbing and descending the mountain except steep slope. Tunnel can be used in crossing foothill. Perpendicularly traversing the river is better than beveling; the worst choice is to put the pipeline along the river. Bypass is the best choice in karsts area. The order of route selection should be pre-choosing, investigation, optimization and adjustment.

Anchorage performance of large-diameter FRP bolts and their application in large deformation roadway

In underground coal mines, fibre reinforced polymer(FRP) bolt is ideal for mined rib reinforcements as it can prevent gas explosions caused by shearer frictional spark. With increasing mining depth, small diameter FRP bolts used in shallow underground mining cannot fulfil the rib support requirements. Under the engineering background of deep underground shortwall mining in Wudong coal mine, this paper systematically studies Φ27 mm FRP bolt support for large deformation coal rib. Specimens with a fan-shaped cross-section were used to enable the tensile testing of the bolt rod, the measured average tensile strength of the studied FRP bolt was(486.1 ± 9.6) MPa with a maximum elongation of 5.7%±0.6%.The shear strength of the bolt was measured as approximately 258 MPa using a self-made double shear testing apparatus. Based on the equivalent radial stiffness principle, a laboratory short encapsulation pullout test(SEPT) method for rib bolting has been developed undertaken consideration of the mechanical properties of the coal seam. Results showed that the average peak anchorage forces of the Φ27 mm FRP bolt and Φ20 mm steel rebar bolt were 108.4 and 66.4 k N, respectively, which were agreed with the theoretical calculations and field measurements. Based on theoretical analysis of the loading states of the bolt under site conditions, bolting method of full-length resin grouting was adopted to offset the weaknesses of the FRP bolt. Numerical method was employed to compare the bolting effect using Φ27 mm FRP bolts and steel rebar bolts. Large diameter FRP bolting was determined as the optimum rib support scheme to increase the productivity of the coal mine and to enhance the ground control capability for+425 level mining roadways. This study provides the laboratory testing design and theoretical prediction of large diameter FRP bolts used for rib support in large deformation roadways.

THE RISK ASSESSMENT OF THE LONG-DISTANCE OILPIPELINE ENGINEERING

The oilfield construction and long-distance oil pipeline engineering has been developed extensively in China. The risk assessment of oil industry will, however, be an important objective to cope with the development of oil industry , The risk assessment of oil industry has many subjects worthy to be studied.The major purpose of the paper is to research the risk cases of long-distance oil pipeline engineering in Ganshu and Shaanxi provinces.