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Hi-Tag:a simple and efficient method for identifying protein-mediated long-range chromatin interactions with low cell numbers
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作者 Xiaolong Qi Lu Zhang +11 位作者 Qiulin Zhao Peng Zhou Sai Xian Zhang Jingjin Li Zhuqing Zheng Yue Xiang Xueting Dai Zhe Jin Yaobang Jian Xinyun Li Liangliang Fu Shuhong Zhao 《Science China(Life Sciences)》 SCIE CAS CSCD 2024年第5期1027-1034,共8页
Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation.However,these techniques require complex experimental procedures and millions of cells pe... Protein-mediated chromatin interactions can be revealed by coupling proximity-based ligation with chromatin immunoprecipitation.However,these techniques require complex experimental procedures and millions of cells per experiment,which limits their widespread application in life science research.Here,we develop a novel method,Hi-Tag,that identifies high-resolution,long-range chromatin interactions through transposase tagmentation and chromatin proximity ligation(with a phosphorothioate-modified linker).Hi-Tag can be implemented using as few as 100,000 cells,involving simple experimental procedures that can be completed within 1.5 days.Meanwhile,Hi-Tag is capable of using its own data to identify the binding sites of specific proteins,based on which,it can acquire accurate interaction information.Our results suggest that Hi-Tag has great potential for advancing chromatin interaction studies,particularly in the context of limited cell availability. 展开更多
关键词 Hi-Tag chromatin interactions transposase tagmentation chromatin proximity ligation low cell numbers
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3C-based methods to detect long-range chromatin interactions
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作者 Gang WEI Keji ZHAO 《Frontiers in Biology》 CSCD 2011年第1期76-81,共6页
Transcriptional regulatory regions are often located several thousand bases from the gene that they control.To function, the chromatin strand forms loops to juxtapose distal regions with the promoter. These long-range... Transcriptional regulatory regions are often located several thousand bases from the gene that they control.To function, the chromatin strand forms loops to juxtapose distal regions with the promoter. These long-rangechromatin interactions have profound influences on the regulation of gene expression and mapping these interactions iscurrently a subject of intensive investigation. Chromosome conformation capture (3C) technology and its derivativeshave been widely used to detect chromatin interactions and greatly contributed to understanding of the relationshipbetween genome organization and genome function. Here we review these 3C-based methods for the study of long-rangechromatin interactions and recent exciting findings obtained by using these technologies. 展开更多
关键词 chromatin interactions 3C gene regulation next-generation sequencing
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THE VLASOV-POISSON-BOLTZMANN SYSTEM NEAR MAXWELLIANS FOR LONG-RANGE INTERACTIONS 被引量:1
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作者 王路生 肖清华 +1 位作者 熊林杰 赵会江 《Acta Mathematica Scientia》 SCIE CSCD 2016年第4期1049-1097,共49页
In this article, we are concerned with the construction of global smooth small-amplitude solutions to the Cauchy problem of the one species Vlasov-Poisson-Boltzmann system near Maxwellians for long-range interactions.... In this article, we are concerned with the construction of global smooth small-amplitude solutions to the Cauchy problem of the one species Vlasov-Poisson-Boltzmann system near Maxwellians for long-range interactions. Compared with the former result obtained by Duan and Liu in [12] for the two species model, we do not ask the initial perturbation to satisfy the neutral condition and our result covers all physical collision kernels for the full range of intermolecular repulsive potentials. 展开更多
关键词 One-species Vlasov-Poisson-Boltzmann system long-range interactions global solutions near Maxwellians time-velocity weighted energy method
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Dynamics of the Random Ising Model with Long-Range Interaction
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作者 CHEN Yuan LI Zhi-Bing FANG Hai HE Shun-Shan SITU Shu-Ping 《Communications in Theoretical Physics》 SCIE CAS CSCD 2001年第10期469-472,共4页
Critical dynamics of the random Ising model with long-range interaction decaying as r-(d+σ) where d is the dimensionality) is studied by the theoretic renormalization-group approach. The system is released to an evol... Critical dynamics of the random Ising model with long-range interaction decaying as r-(d+σ) where d is the dimensionality) is studied by the theoretic renormalization-group approach. The system is released to an evolution within a model A dynamics. Asymptotic scaling laws are studied in a frame of the expansion in = 2σ - d. In dimensions d < 2σ. the dynamic exponent z is calculated to the second order in at the random fixed point. 展开更多
关键词 ISING model critical dynamics long-range interaction quenched IMPURITIES
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Lagrangian formulation of continuum with internal long-range interactions
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作者 Zaixing Huang a) College of Aerospace Engineering,Nanjing University of Aeronautics and Astronautics,Nanjing 210016,China 《Theoretical & Applied Mechanics Letters》 CAS 2011年第4期60-63,共4页
Based on a new definition of nonlocal variable,this paper establishes the Lagrangian formulation for continuum with internal long-range interactions.Distinguished from the existing theories,the nonlocal term in the La... Based on a new definition of nonlocal variable,this paper establishes the Lagrangian formulation for continuum with internal long-range interactions.Distinguished from the existing theories,the nonlocal term in the Lagrangian formulation automatically satisfies the zero mean condition determined by the action and reaction law.By this formulation,elastic wave in a rod with the internal long-range interactions is investigated.The dispersion of the elastic wave is predicted. 展开更多
关键词 nonlocal mechanics Lagrangian formulation long-range interactions physically-based nonlocal model
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Some Thoughts upon Long-Range Interaction and Entangled States
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作者 Bo Lehnert 《Journal of Electromagnetic Analysis and Applications》 2018年第12期193-196,共4页
The problems of long-range interaction and associated questions on entangled states are reconsidered in terms of a recently developed revised quantum electrodynamic theory by the author, as being applied to subatomic ... The problems of long-range interaction and associated questions on entangled states are reconsidered in terms of a recently developed revised quantum electrodynamic theory by the author, as being applied to subatomic systems. There are indications that the theories of relativity and quantum mechanics do not necessarily have to be in conflict. But more investigations are required for a full understanding to be obtained on these problems. 展开更多
关键词 Revised Quantum ELECTRODYNAMICS Standard Model and Beyond ZERO Point Energy long-range interaction ENTANGLED STATES
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Screening and functional analysis of the long-range interaction elements ofβ-globin genes
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作者 XIAO Yi-shu XU Lan +4 位作者 LIU Chun-ya DU Le SUN Yuan-tian LI Dong-na REN Li-cheng 《Journal of Hainan Medical University》 2022年第24期1-7,共7页
Objective:Studies have shown thatβ-globin gene presents a selective expression transformation mechanism during development,and its upstream locus control region(LCR)regulates the expression pattern ofβ-globin gene f... Objective:Studies have shown thatβ-globin gene presents a selective expression transformation mechanism during development,and its upstream locus control region(LCR)regulates the expression pattern ofβ-globin gene family.To further explore the molecular network ofβ-globin gene expression regulation,other long-range regulatory elements that may be involved in the regulation ofβ-globin gene expression were screened and the dynamic regulation and transformation mechanism ofβ-globin gene was deeply studied.Methods:Promyelocytic cells were induced to differentiate by all-trans retinoic acid.β-globin gene promoter region and LCR were used as the target sites for circular chromosome conformational capture(4C)analysis.Through sequencing and regulatory element analysis,the sites interacting withβ-globin family loci were screened in the whole genome.Results:According to the results of 4C sequencing,the sites that interact with HBD promoter region and LCR were screened.Verified by chromosome conformational capture(3C),the results were consistent with those of sequencing.The functional analysis of regulatory elements by formaldehyde-assisted separation regulatory elements and Epiregio online website showed that the screening sites AC105129.4,AL354707.17,AC078785.22 and AC021646.35 were all potential regulatory elements involved inβ-globin gene.Conclusion:The interaction between 4C screening site and anchor site showed the complex spatial organization ofβ-globin family loci in the nucleus. 展开更多
关键词 β-Globin gene Circular chromosome conformation capture chromatin interaction Chromosome conformation capture
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Transcription's bubble under the influence of long-range interactions and helicoidal coupling
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作者 Mirabeau Saha Timolon C.Kofan 《Chinese Physics B》 SCIE EI CAS CSCD 2013年第12期589-602,共14页
The influence of power-low long-range interactions (LRI) and helicoidal coupling (HC) on the properties of localized solitons in a DNA molecule when a ribonucleic acid polymerase (RNAP) binds to it at the physio... The influence of power-low long-range interactions (LRI) and helicoidal coupling (HC) on the properties of localized solitons in a DNA molecule when a ribonucleic acid polymerase (RNAP) binds to it at the physiological temperature is analytically and numerically investigated in this paper. We have made an analogy with the Heisenberg model Hamiltonian of an anisotropic spin ladder with ferromagnetic legs and anti-ferromagnetic rung coupling. When we limit ourselves to the second-order terms in the Taylor expansion, the DNA dynamics is found to be governed by a completely integrable nonlinear Schr?dinger (NLS) equation. In this case, results show that increasing the value of HC force or LRI parameter enhances the bubble height and reduces the number of base pairs which form the bubble. For the fourth-order terms in a Taylor expansion, results are closely resembling those of second-order terms, and are confirmed by numerical investigation. These results match with some experimental data and thus provide a better representation of the base pairs opening in DNA which is essential for the transcription process. 展开更多
关键词 DNA helicoidal coupling RNA-polymerase spin model Riemann zeta function power-law long-range interaction breather-like soliton
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Dynamical Stability of Gap Soliton of One-Dimensional Condensate in Optical Lattices with Strong Interatomic Interaction 被引量:2
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作者 王登龙 颜晓红 王凤姣 《Chinese Physics Letters》 SCIE CAS CSCD 2007年第7期1817-1820,共4页
By developing the multiple scales method, we analytically study the dynamics properties of gap soliton of Bose- Einstein condensate in optical lattices. It is shown that the gap soliton will appear at Brillouin zone e... By developing the multiple scales method, we analytically study the dynamics properties of gap soliton of Bose- Einstein condensate in optical lattices. It is shown that the gap soliton will appear at Brillouin zone edge of linear band spectrum of the condensates when the interatomic interaction strength is larger than the lattice depth. Moreover, the density of gap soliton starts to be relatively small, while it increases with time and becomes stable. 展开更多
关键词 BOSE-EINSTEIN CONDENSATE long-range interaction ENVELOPE CHAIN
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Short—Time Critical Behavior Affected by Weakly Long—Range Interactions
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作者 CHENYuan LIZhi-Bing 《Communications in Theoretical Physics》 SCIE CAS CSCD 2001年第4期475-479,共5页
The theoretic renormalization group approach is applied to the study of short-time critical behavior of the Ginzburg–Landau model with weakly long-range interactions . The system initially at a high temperature is fi... The theoretic renormalization group approach is applied to the study of short-time critical behavior of the Ginzburg–Landau model with weakly long-range interactions . The system initially at a high temperature is firstly quenched to the critical temperature and then released to an evolution with a model A dynamics. A double expansion in and with of order is employed, where is the spatial dimension. The asymptotic scaling laws and the initial slip exponents and for the order parameter and the response function respectively are calculated to the second order in for close to 2. 展开更多
关键词 Ginzburg-Landau model short-time critical dynamics weakly long-range interactions
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Spin waves and phase transition on a magnetically frustrated square lattice with long-range interactions
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作者 谭宇婷 姚道新 《Frontiers of physics》 SCIE CSCD 2023年第3期71-80,共10页
We investigate the effects of long-range interactions on the spin wave spectra and the competition between magnetic phases on a frustrated square lattice with large spin S.Applying the spin wave theory and assisted wi... We investigate the effects of long-range interactions on the spin wave spectra and the competition between magnetic phases on a frustrated square lattice with large spin S.Applying the spin wave theory and assisted with symmetry analysis,we obtain analytical expressions for spin wave spectra of competing Neel and(π,0)stripe states of systems containing anyorder long-range interactions.In the specific case of long-range interactions with power-law decay,we find surprisingly that the staggered long-range interaction suppresses quantum fluctuation and enlarges the ordered moment,especially in the Neel state,and thus extends its phase boundary to the stripe state.Our findings illustrate the rich possibilities of the roles of long-range interactions,and advocate future investigations in other magnetic systems with different structures of interactions. 展开更多
关键词 spin wave phase transition FRUSTRATION square lattice long-range interaction ANTIFERROMAGNET
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Conserved H3K27me3-associated chromatin looping mediates physical interactions of gene clusters in plants 被引量:2
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作者 Linhua Sun Yuxin Cao +5 位作者 Zhu Li Yi Liu Xiaochang Yin Xing Wang Deng Hang He Weiqiang Qian 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2023年第8期1966-1982,共17页
Higher-order chromatin organization is essential for transcriptional regulation,genome stability maintenance,and other genome functions.Increasing evidence has revealed significant differences in 3D chromatin organiza... Higher-order chromatin organization is essential for transcriptional regulation,genome stability maintenance,and other genome functions.Increasing evidence has revealed significant differences in 3D chromatin organization between plants and animals.However,the extent,pattern,and rules of chromatin organization in plants are still unclear.In this study,we systematically identified and characterized long-range chromatin loops in the Arabidopsis 3D genome.We identified hundreds of long-range cis chromatin loops and found their anchor regions are closely associated with H3K27me3 epigenetic modifications.Furthermore,we demonstrated that these chromatin loops are dependent on Polycomb group(PcG)proteins,suggesting that the Polycomb repressive complex2(PRC2)complex is essential for establishing and maintaining these novel loops.Although most of these PcG-medicated chromatin loops are stable,many of these loops are tissue-specific or dynamically regulated by different treatments.Interestingly,tandemly arrayed gene clusters and metabolic gene clusters are enriched in anchor regions.Long-range H3K27me3-marked chromatin interactions are associated with the coregulation of specific gene clusters.Finally,we also identified H3K27me3-associated chromatin loops associated with gene clusters in Oryza sativa and Glycine max,indicating that these long-range chromatin loops are conserved in plants.Our results provide novel insights into genome evolution and transcriptional coregulation in plants. 展开更多
关键词 ARABIDOPSIS gene clusters Hi-C long-range chromatin loops H3K27me3
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Mapping Multi-factor-mediated Chromatin Interactions to Assess Dysregulation of Lung Cancer-related Genes
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作者 Yan Zhang Jingwen Zhang +6 位作者 Wei Zhang Mohan Wang Shuangqi Wang Yao Xu Lun Zhao Xingwang Li Guoliang Li 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2023年第3期573-588,共16页
Studies on the lung cancer genome are indispensable for developing a cure for lung cancer.Whole-genome resequencing,genome-wide association studies,and transcriptome sequencing have greatly improved our understanding ... Studies on the lung cancer genome are indispensable for developing a cure for lung cancer.Whole-genome resequencing,genome-wide association studies,and transcriptome sequencing have greatly improved our understanding of the cancer genome.However,dysregulation of longrange chromatin interactions in lung cancer remains poorly described.To better understand the three-dimensional(3D)genomic interaction features of the lung cancer genome,we used the A549 cell line as a model system and generated high-resolution chromatin interactions associated with RNA polymerase II(RNAPII),CCCTC-binding factor(CTCF),enhancer of zeste homolog 2(EZH2),and histone 3 lysine 27 trimethylation(H3K27me3)using long-read chromatin interaction analysis by paired-end tag sequencing(ChIA-PET).Analysis showed that EZH2/H3K27me3-mediated interactions further repressed target genes,either through loops or domains,and their distributions along the genome were distinct from and complementary to those associated with RNAPII.Cancer-related genes were highly enriched with chromatin interactions,and chromatin interactions specific to the A549 cell line were associated with oncogenes and tumor suppressor genes,such as additional repressive interactions on FOXO4 and promoter–promoter interactions between NF1 and RNF135.Knockout of an anchor associated with chromatin interactions reversed the dysregulation of cancer-related genes,suggesting that chromatin interactions are essential for proper expression of lung cancer-related genes.These findings demonstrate the 3D landscape and gene regulatory relationships of the lung cancer genome. 展开更多
关键词 Lung cancer 3D genome ChIA-PET chromatin interaction DYSREGULATION
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A Classification of Long-Range Interactions between Two Stacks of p& p'-Branes
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作者 欧阳君 吴超 《Communications in Theoretical Physics》 SCIE CAS CSCD 2015年第2期195-208,共14页
We generalize the computations of the long-range interactions between two parallel stacks of branes to various cases when two stacks of branes are not placed parallel to each other. We classify the nature of interacti... We generalize the computations of the long-range interactions between two parallel stacks of branes to various cases when two stacks of branes are not placed parallel to each other. We classify the nature of interaction(repulsive or attractive) for each special case and this classification can be used to justify the nature of long-range interaction between two complicated brane systems such as brane bound states. We will provide explicit examples in this paper to demonstrate this. 展开更多
关键词 long-range interaction effective field theory computation non-parallel branes FLUX
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雷帕霉素诱导β-珠蛋白家族基因座位重塑调控该基因的转换表达
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作者 许兰 肖亦舒 +4 位作者 刘春亚 贾炳豪 杜乐 李冬娜 任立成 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2023年第9期1322-1331,共10页
β-珠蛋白基因编码异常导致的β-地中海贫血是许多亚洲国家最常见的血红蛋白病。深入研究珠蛋白基因表达的分子基础和表观遗传机制,是探索治疗地中海贫血新方案的关键。本研究利用FAIRE、3C及ChIP等主要技术方法,探讨雷帕霉素诱导CD4^(... β-珠蛋白基因编码异常导致的β-地中海贫血是许多亚洲国家最常见的血红蛋白病。深入研究珠蛋白基因表达的分子基础和表观遗传机制,是探索治疗地中海贫血新方案的关键。本研究利用FAIRE、3C及ChIP等主要技术方法,探讨雷帕霉素诱导CD4^(+)T细胞核内染色质重塑过程中,β-珠蛋白家族基因座位的三维相互作用网络及其重塑在功能上调控基因表达的分子机制。结果显示,雷帕霉素处理浓度从低到高的变化过程中,珠蛋白基因染色质的开放程度、基因启动子区与调控元件LCR之间的相互作用频率以及CTCF在基因启动子区的富集效率发生不同的改变,这种变化导致了基因表达模式也呈现相同的变化趋势。10 nmol/L浓度处理时,染色质可及性降低,基因表达下降(P<0.05);20 nmol/L和50 nmol/L浓度时,染色质可及性增加,基因表达上调(P<0.05)。本研究通过这种动态的变化过程阐述了β-珠蛋白家族基因表达转换调控的分子机制,为临床精准治疗提供了理论与临床实践基础。 展开更多
关键词 β-珠蛋白家族基因 染色质重塑 染色质相互作用 染色质可及性 基因表达调控
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The local density of H3K9me3 dictates the stability of HP1α condensates-mediated genomic interactions
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作者 Ying Feng Li Guo +3 位作者 Chen Yang Hui Zheng Xiao Xiao Hanhui Ma 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2023年第10期776-785,共10页
The human genome can be demarcated into domains based on distinct epigenetic states.The trimethylation of histone H3 lysine 9(H3K9me3)is essential for the formation of constitutive heterochromatin nanodomains.However,... The human genome can be demarcated into domains based on distinct epigenetic states.The trimethylation of histone H3 lysine 9(H3K9me3)is essential for the formation of constitutive heterochromatin nanodomains.However,the extent to which genomic regions require specific densities or degrees of H3K9me3 for stable interactions remains unclear.Here,we utilize CRISPR-based DNA imaging to investigate the role of endogenous or ectopic H3K9me3 in chromatin dynamics and genomic interactions.We select three loci(IDR3,TCF3,and PR1)with distinct levels of H3K9me3 to examine the genomic interactions and association with endogenous Heterochromatin Protein 1(HP1α)condensates.Our results demonstrate a positive correlation between the levels of H3K9me3 at the loci and their association with HP1αcondensates.By dual-color labeling and long-term tracking of IDR3 and PR1 loci,we find a periodical association between the two ranging from one to three hours.Epigenetic perturbation-induced Genome organization(EpiGo)-KRAB introduces∼20 kilobases of H3K9me3 at the TCF3 locus,which is sufficient to establish a stable association between TCF3 and HP1αcondensates.In addition,EpiGo-mediated H3K9me3 also leads to stable genomic interaction between IDR3 and TCF3.Briefly,these data suggest that the density of H3K9me3 could dictate the stability of interactions between genomic loci and HP1αcondensates. 展开更多
关键词 H3K9me3 HP1α chromatin dynamics Genomic interaction Epigenetic perturbation
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Multiple functions of SWI/SNF chromatin remodeling complex in plant-pathogen interactions 被引量:1
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作者 Yunqing Jian Won-Bo Shim Zhonghua Ma 《Stress Biology》 CAS 2021年第1期215-229,共15页
The SWI/SNF chromatin remodeling complex utilizes the energy of ATP hydrolysis to facilitate chromatin access and plays essential roles in DNA-based events.Studies in animals,plants and fungi have uncovered sophistica... The SWI/SNF chromatin remodeling complex utilizes the energy of ATP hydrolysis to facilitate chromatin access and plays essential roles in DNA-based events.Studies in animals,plants and fungi have uncovered sophisticated regulatory mechanisms of this complex that govern development and various stress responses.In this review,we summarize the composition of SWI/SNF complex in eukaryotes and discuss multiple functions of the SWI/SNF complex in regulating gene transcription,mRNA splicing,and DNA damage response.Our review further highlights the importance of SWI/SNF complex in regulating plant immunity responses and fungal pathogenesis.Finally,the potentials in exploiting chromatin remodeling for management of crop disease are presented. 展开更多
关键词 chromatin remodeling SWI/SNF complex TRANSCRIPTION SPLICING DNA damage repair Plant-pathogen interaction
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Tn5-FISH,a novel cytogenetic method to image chromatin interactions with sub-kilobase resolution
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作者 Jing Niu Xu Zhang +8 位作者 Li Guipeng Pixi Yan Qing Yan Qionghai Dai Dayong Jin Xiaohua Shen Jichang Wang Michael QZhang Juntao Gao 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2020年第12期727-734,共8页
There is an increasing interest in understanding how three-dimensional(3D)organization of the genome is regulated.Different strategies have been employed to identify genome-wide chromatin interactions.However,due to c... There is an increasing interest in understanding how three-dimensional(3D)organization of the genome is regulated.Different strategies have been employed to identify genome-wide chromatin interactions.However,due to current limitations in resolving genomic contacts,visualization and validation of these genomic loci with sub-kilobase resolution remain unsolved to date.Here,we describe Tn5 transposase-based Fluorescencein situhybridization(Tn5-FISH),a PCR-based,cost-effective imaging method,which can co-localize the genomic loci with sub-kilobase resolution,dissect genome architecture,and verify chromatin interactions detected by chromatin configuration capture(3C)-derived methods.To validate this method,short-range interactions in keratin-encoding gene(KRT)locus in topologically associated domain(TAD)were imaged by triple-color Tn5-FISH,indicating that Tn5-FISH is very useful to verify short-range chromatin interactions inside the contact domain and TAD.Therefore,Tn5-FISH can be a powerful molecular tool for the clinical detection of cytogenetic changes in numerous genetic diseases such as cancers. 展开更多
关键词 Fluorescencein situhybridization chromatin interaction Cellular imaging Super resolution
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核酸-蛋白质互作的生物化学研究方法 被引量:6
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作者 张金璧 潘增祥 +2 位作者 林飞 马雪山 刘红林 《遗传》 CAS CSCD 北大核心 2009年第3期325-336,共12页
研究核酸-蛋白质的互作是揭示生命活动机理的基础,文章简要综述了用于研究核酸-蛋白质互作的各种生物化学方法。从体内、体外两个研究角度,针对核酸、蛋白以及复合物3个研究水平,概述了硝化纤维膜过滤实验、足迹法、EMSA、Southwestern... 研究核酸-蛋白质的互作是揭示生命活动机理的基础,文章简要综述了用于研究核酸-蛋白质互作的各种生物化学方法。从体内、体外两个研究角度,针对核酸、蛋白以及复合物3个研究水平,概述了硝化纤维膜过滤实验、足迹法、EMSA、Southwestern杂交等经典分析方法的原理、发展和运用。还着重介绍了最近在表观遗传学领域中广泛运用的nChIP、xChIP等基本染色质免疫沉淀(ChIP)技术及其衍生出的ChIP-on-chip等方法。 展开更多
关键词 核酸 蛋白质 互作 足迹 染色质免疫沉淀
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染色质免疫沉淀技术在研究DNA与蛋白质相互作用中的应用 被引量:5
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作者 王春雨 石建党 +1 位作者 朱彦 张琚 《遗传》 CAS CSCD 北大核心 2005年第5期801-807,共7页
在后基因组时代,DNA-蛋白质的相互作用是研究基因表达调控的一个重要领域。与其他方法相比,染色质免疫沉淀技术(chromatin immunoprecipitation assay,ChIP)是一种在体内研究DNA-蛋白质相互作用的理想的方法。近年来这种方法与DNA芯片... 在后基因组时代,DNA-蛋白质的相互作用是研究基因表达调控的一个重要领域。与其他方法相比,染色质免疫沉淀技术(chromatin immunoprecipitation assay,ChIP)是一种在体内研究DNA-蛋白质相互作用的理想的方法。近年来这种方法与DNA芯片和分子克隆技术相结合,可用于高通量的筛选已知蛋白因子的未知DNA靶点和研究反式作用因子在整个基因组上的分布情况,这将有助于深入理解DNA-蛋白质相互作用的调控网络。总结了染色质免疫沉淀技术的方法,特别介绍了使用这些方法取得的最新进展。 展开更多
关键词 DNA-蛋白质相互作用 染色质免疫沉淀技术(ChIP) DNA芯片 ChIP-克隆
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