This paper studied the effect of reducing application of chemical fertilizer,increasing application of functional organic fertilizer and trace elements and fully using biological pesticides on physiological traits,yie...This paper studied the effect of reducing application of chemical fertilizer,increasing application of functional organic fertilizer and trace elements and fully using biological pesticides on physiological traits,yield and quality of radish. The results showed that applying functional organic fertilizer and trace elements and fully using biological pesticides could help to significantly improve the radish fleshy root yield,increase soluble sugar and vitamin C content,reduce crude fiber and nitrate content,but had no significant influence on radish moisture content,glucosinolates and chlorpyrifos content in organophosphorus pesticides.展开更多
The residual effect of organic and mineral fertilizers on selected chemical properties of an Alfisol in the long term soil fertility trial established in 1950 at Samaru, Nigerian savanna was assessed. The trial was le...The residual effect of organic and mineral fertilizers on selected chemical properties of an Alfisol in the long term soil fertility trial established in 1950 at Samaru, Nigerian savanna was assessed. The trial was left fallow for fourteen years due to lack of funds. Topsoil was collected from plots that received three levels of cow dung (D), nitrogen (N) and phosphorus (P) in all possible combinations. The soils were analyzed for selected chemical properties. Except for significant effect of applied P on available P, sole application of cow dung, N or P had no significant effect on all the measured soil properties. The interaction of D and P significantly affected the mean values of exchangeable Ca, Mg, K and ECEC in the range of 1.12-1.96, 0.62-1.11, 0.37-0.64 and 2.82-4.11 cmol/kg respectively. The most important results were the ability of the plot that received neither D nor P to significantly increase these parameters than the plots that received only one of the treatments. The results show that the fallow period has changed the effects of application of organic and mineral fertilizers on the soil chemical properties under continuous cultivation by modifying them towards those of a native savanna Alfisol.展开更多
Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied. The experiments on long-term application of K fertiliz...Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied. The experiments on long-term application of K fertilizer and wheat straw to soil in Hebei fluvo aquic soil and Shanxi brown soil in northern China were begun in 1992. The results showed that K fertilizer and straw could improve the yields of wheat and maize with the order of NPK + St 〉 NPK 〉 NP + St 〉 NP, and treatment of K fertilizer made a significant difference to NP, and the efficiency of K fertilizer in maize was higher than in wheat under rotation system of Hebei. In contrast with Shanxi, the wastage of soil potassium was a more serious issue in the rotation system in Hebei, only treatment of NPK + St showed a surplus of potassium and the others showed a wane. K fertilizer and straw could improve the content of water-soluble K, nonspecifically adsorbed K, non-exchangeable K, mineral K, and total K in contrast to NP; however, K fertilizer and straw reduce the proportion of mineral K and improve proportion of other forms of potassium in the two locating sites. Compared with the beginning of orientation, temporal variability character of soil K content and proportion showed a difference between the two soil types; furthermore, there was a decrease in the content of mineral K and total K simultaneously in the two locating sites. As a whole, the effect of K fertilizer applied to soil directly excelled to wheat straw to soil. Wheat straw to soil was an effective measure to complement potassium to increase crop yield and retard the decrease of soil K.展开更多
Application of nitrogen (N) fertilizer is one of the most important approaches on improving maize grain yield. However, as is known to all, overuse N fertilizer not only leads to decline of N use efficiency and maize ...Application of nitrogen (N) fertilizer is one of the most important approaches on improving maize grain yield. However, as is known to all, overuse N fertilizer not only leads to decline of N use efficiency and maize yield, but also leads to potential risk to environment pollution. This experiment was conducted to determine the effects of N fertilizer applications with nine different treatments on soil physical-chemical characters and maize grain yield using hybrid variety Zhengdan 958 in 2011 and 2012. Results indicated that the soil bulk densities of T2 (CK) and T1 were the lowest compared to other treatments in 2011 and 2012, respectively, whereas the soil bulk density of T5 in 2011 and T3 in 2012 were higher than other treatments. The soil porosity and field capacity of T5 in 2011 and T3 in 2012 were lower than other treatments, but those of CK in 2011 and T1 in 2012 were higher than other treatments. The pH values of T3 to T7 were lower than other treatments. These results indicated that the soil bulk densities were increased, whereas the soil porosity, field capacity and values pH were decreased by N application at different stages. N application could increase the N contents of leaf and stem, whereas less or excess N application should not significant improve maize yield. Although the soil organic matter and total N contents of T3 were the highest in both 2011 and 2012, the yield of T4 is the highest in both 2011 and 2012. The application amount, period and times of N fertilizer were important to maize yield.展开更多
In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of ...In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of nitrogen and potassium fertilizers combined with returning Chinese milk vetch and straw to fields on yield,fertilizer utilization efficiency,net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci),chlorophyll content(SPAD value)and soil physical and chemical properties in late rice harvest period.The results showed that the optimized application of nitrogen and potassium fertilizers combined with the integrated technology of Chinese milk vetch and straw co-returning to the field could enhance the photosynthetic efficiency of double-cropping rice,increase rice yield,and enhance soil biological activity,especially T4 treatment involving the returning of Chinese milk vetch and straw to the field instead of 30%nitrogen fertilizer achieved the highest rice yield,fertilizer use efficiency,net photosynthetic rate and soil biological activity.Compared with the conventional fertilization treatment T2,the total rice yield of T4 treatment increased by 4.1%,among which the early rice and late rice increased by 6.3%and 2.4%,respectively;Pn,Gs and SPAD values of flag leaves at full heading stage significantly increased,and the contents of soil active organic carbon,alkali hydrolyzed nitrogen,available phosphorus and readily available potassium significantly increased.展开更多
In order to illustrate the change of nitrogen (N) supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the ...In order to illustrate the change of nitrogen (N) supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the soil-climatic conditions of the North China Plain, organic N forms were quantified in the topsoil with different manure and chemical fertilizer treatments in a 15-year fertilizer experiment in a Chinese calcareous alluvial soil. Soil total N (TN) and various organic N forms were significantly influenced by long-term application of chemical fertilizer and manure. TN, total hydrolysable N, acid-lnsoluble N, amino acid N and ammonium N in the soil increased significantly (P 〈 0.05) with increasing manure and fertilizer N rates, but were not influenced by increasing P rates. Also, application of manure or N fertilizer or P fertilizer did not significantly influence either the quantity of amino sugar N or its proportion of TN. Application of manure significantly increased (P 〈 0.05) hydrolysable unknown N, but adding N or P did not. In addition, application of manure or N fertilizer or P fertilizer did not significantly influence the proportions of different soil organic N forms.展开更多
Human activities have altered weather patterns by causing an increase in greenhouse gas. The effects of climate change have been studied, including effects on some ecosystems throughout the world. There are many studi...Human activities have altered weather patterns by causing an increase in greenhouse gas. The effects of climate change have been studied, including effects on some ecosystems throughout the world. There are many studies on changes in the soil due to climate change, but much of them did not extend their research to soil enzyme that integrates information on soil microbial status and soil physical-chemical conditions. Meanwhile, there are lots of experimental fields established to study effects of long-term fertilization on soil enzyme activities, but many did not compare the difference of soil enzyme activities and did not analyze the effect of climatic factors on soil enzyme activities with long-term fertilization under different hydrothermal conditions. In this study, we compared soil enzyme activities of three long-fertilization stations which had different hydrothermal conditions in Northeast China, and analyzed the relationship of hydrothermal condition, soil chemical properties with soil enzyme activities. Hydrothermal conditions (annual temperature and total rainfall) decreased in order of Gongzhuling (Jilin Province, China ) Harbin (Heilongjiang Province, China) Heihe (Heilongjiang Province, China) over the course of the long-term fertilization experiment. Sunshine hours showed the longest in Gongzhuling, the second in Heihe, and the last in Harbin. However, the order of soil enzymes was not in agreement with hydrothermal conditions. Overall, the order of soil enzymes for the same treatment among three stations was consistent in 2008 with in 2009. Correlation analysis demonstrated that different soil enzymes achieved the different affected levels by climatic factors under different fertilization treatments. Urease activity showed a significant relationship with sunshine hours in no fertilizer (CK) treatment (R=-0.91, P0.01) and relative humidity in mineral fertilizers plus manure (MNPK) treatment (R=0.82, P0.05). Phosphatase activity exhibited a negative correlation with annual mean temperature, annual mean maximum temperature and annual mean minimum temperature, and their correlation coefficients were separately -0.83, -0.79, and -0.83 at P0.05 in CK treatment. Invertase activity was highly and positively correlated with sunshine hours in CK treatment (R=0.94, P0.01). Catalase activity showed significant negative correlations with minimum relative humidity in CK treatment (R=-0.81, P0.05), and positive correlations with sunshine hours in M treatment (R=0.83, P0.05). There were no climatic factors which strongly affected on dehydrogenase in all treatments. Soil enzyme activities were closely related to the soil chemical properties. Soil urease activity was positively correlated with available P (P0.05). With exception of correlation between invertase and total P at P0.05, phosphase, invertase, catalase, and dehydrogenase showed significant positive correlations with soil chemical properties (P0.01). It was a comprehensive process that biologic and abiotic factors were effect on soil enzyme activities under different fertilization treatments. To sum up, the variation of hydrothermal conditions in different climate zones and soil chemical properties affect integrally metabolic activity and metabolic finger print of microbial communities in black soil.展开更多
Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. ...Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates. The results showed that if chemical fertilizers of N, P and K were applied in balance, soil p H decreased very slightly. There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment(the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals(CI) with a mean of 1.23), mainly due to the increasing soil total N content. The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment. However, long-term chemical fertilizer application did not significantly affect the gross NH4+ immobilization rate, but accelerated gross nitrification rate(1.19; 95% CI: 1.08 to 1.31). Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment. Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.展开更多
为了研究化肥减量配施有机肥对木薯根际土壤细菌多样性和群落结构及产量的影响,采用Illumina Nova 6000测序平台,对未施肥(CK)、有机肥(T_(1))、常规施肥+有机肥(T_(2))和化肥减施+有机肥(T_(3))等施肥方式处理下的华南12号木薯根际土...为了研究化肥减量配施有机肥对木薯根际土壤细菌多样性和群落结构及产量的影响,采用Illumina Nova 6000测序平台,对未施肥(CK)、有机肥(T_(1))、常规施肥+有机肥(T_(2))和化肥减施+有机肥(T_(3))等施肥方式处理下的华南12号木薯根际土壤进行16S rRNA高通量测序分析。结果表明,与CK相比,T_(3)处理不仅能显著增加木薯株高和茎粗,还能提高木薯产量。在相似水平为97%下聚类分析得到木薯根际土壤OTUs,分别为3603个(CK)、1688个(T_(1))、1276个(T_(2))和3317个(T_(3))。不同施肥方式改变土壤细菌的多样性和丰度,其物种多样性由高到低依次为:CK>T_(3)>T_(1)>T_(2)。基于土壤细菌群落的PCoA和聚类分析,CK和T_(3)细菌群落结构组成较为相似,T_(1)与T_(2)细菌群落结构组成相似。不同施肥处理下木薯根际土壤中优势菌门为变形菌门、拟杆菌门、酸杆菌门、厚壁菌门、放线菌门和绿弯菌门。化肥减施配施有机肥增加了变形菌门、拟杆菌门和放线菌门的相对丰度,降低了厚壁菌门和绿弯菌门的相对丰度。冗余性分析结果表明速效钾是影响木薯根际土壤菌群的主要效应因子。研究表明,化肥减量配施有机肥不仅能增加木薯的产量,还能改变木薯根际土壤细菌群落结构和多样性,为发展绿色、高效、可持续的木薯产业提供坚实的理论支撑。展开更多
基金Supported by the Second Batch of Modern Agricultural Industry Technology System Project in Hubei Province
文摘This paper studied the effect of reducing application of chemical fertilizer,increasing application of functional organic fertilizer and trace elements and fully using biological pesticides on physiological traits,yield and quality of radish. The results showed that applying functional organic fertilizer and trace elements and fully using biological pesticides could help to significantly improve the radish fleshy root yield,increase soluble sugar and vitamin C content,reduce crude fiber and nitrate content,but had no significant influence on radish moisture content,glucosinolates and chlorpyrifos content in organophosphorus pesticides.
文摘The residual effect of organic and mineral fertilizers on selected chemical properties of an Alfisol in the long term soil fertility trial established in 1950 at Samaru, Nigerian savanna was assessed. The trial was left fallow for fourteen years due to lack of funds. Topsoil was collected from plots that received three levels of cow dung (D), nitrogen (N) and phosphorus (P) in all possible combinations. The soils were analyzed for selected chemical properties. Except for significant effect of applied P on available P, sole application of cow dung, N or P had no significant effect on all the measured soil properties. The interaction of D and P significantly affected the mean values of exchangeable Ca, Mg, K and ECEC in the range of 1.12-1.96, 0.62-1.11, 0.37-0.64 and 2.82-4.11 cmol/kg respectively. The most important results were the ability of the plot that received neither D nor P to significantly increase these parameters than the plots that received only one of the treatments. The results show that the fallow period has changed the effects of application of organic and mineral fertilizers on the soil chemical properties under continuous cultivation by modifying them towards those of a native savanna Alfisol.
文摘Effect of application of K fertilizer and wheat straw to soil on crop yield and status of soil K in the plough layer under different planting systems was studied. The experiments on long-term application of K fertilizer and wheat straw to soil in Hebei fluvo aquic soil and Shanxi brown soil in northern China were begun in 1992. The results showed that K fertilizer and straw could improve the yields of wheat and maize with the order of NPK + St 〉 NPK 〉 NP + St 〉 NP, and treatment of K fertilizer made a significant difference to NP, and the efficiency of K fertilizer in maize was higher than in wheat under rotation system of Hebei. In contrast with Shanxi, the wastage of soil potassium was a more serious issue in the rotation system in Hebei, only treatment of NPK + St showed a surplus of potassium and the others showed a wane. K fertilizer and straw could improve the content of water-soluble K, nonspecifically adsorbed K, non-exchangeable K, mineral K, and total K in contrast to NP; however, K fertilizer and straw reduce the proportion of mineral K and improve proportion of other forms of potassium in the two locating sites. Compared with the beginning of orientation, temporal variability character of soil K content and proportion showed a difference between the two soil types; furthermore, there was a decrease in the content of mineral K and total K simultaneously in the two locating sites. As a whole, the effect of K fertilizer applied to soil directly excelled to wheat straw to soil. Wheat straw to soil was an effective measure to complement potassium to increase crop yield and retard the decrease of soil K.
文摘Application of nitrogen (N) fertilizer is one of the most important approaches on improving maize grain yield. However, as is known to all, overuse N fertilizer not only leads to decline of N use efficiency and maize yield, but also leads to potential risk to environment pollution. This experiment was conducted to determine the effects of N fertilizer applications with nine different treatments on soil physical-chemical characters and maize grain yield using hybrid variety Zhengdan 958 in 2011 and 2012. Results indicated that the soil bulk densities of T2 (CK) and T1 were the lowest compared to other treatments in 2011 and 2012, respectively, whereas the soil bulk density of T5 in 2011 and T3 in 2012 were higher than other treatments. The soil porosity and field capacity of T5 in 2011 and T3 in 2012 were lower than other treatments, but those of CK in 2011 and T1 in 2012 were higher than other treatments. The pH values of T3 to T7 were lower than other treatments. These results indicated that the soil bulk densities were increased, whereas the soil porosity, field capacity and values pH were decreased by N application at different stages. N application could increase the N contents of leaf and stem, whereas less or excess N application should not significant improve maize yield. Although the soil organic matter and total N contents of T3 were the highest in both 2011 and 2012, the yield of T4 is the highest in both 2011 and 2012. The application amount, period and times of N fertilizer were important to maize yield.
文摘In order to explore the technology and effects of reducing nitrogen and potassium fertilizer applications in double-cropping rice,a field plot experiment was conducted to study the effects of optimized application of nitrogen and potassium fertilizers combined with returning Chinese milk vetch and straw to fields on yield,fertilizer utilization efficiency,net photosynthetic rate(Pn),stomatal conductance(Gs),intercellular CO_(2) concentration(Ci),chlorophyll content(SPAD value)and soil physical and chemical properties in late rice harvest period.The results showed that the optimized application of nitrogen and potassium fertilizers combined with the integrated technology of Chinese milk vetch and straw co-returning to the field could enhance the photosynthetic efficiency of double-cropping rice,increase rice yield,and enhance soil biological activity,especially T4 treatment involving the returning of Chinese milk vetch and straw to the field instead of 30%nitrogen fertilizer achieved the highest rice yield,fertilizer use efficiency,net photosynthetic rate and soil biological activity.Compared with the conventional fertilization treatment T2,the total rice yield of T4 treatment increased by 4.1%,among which the early rice and late rice increased by 6.3%and 2.4%,respectively;Pn,Gs and SPAD values of flag leaves at full heading stage significantly increased,and the contents of soil active organic carbon,alkali hydrolyzed nitrogen,available phosphorus and readily available potassium significantly increased.
基金Project supported by the National Natural Science Foundation of China (Nos. 30390080 and 30370287).
文摘In order to illustrate the change of nitrogen (N) supply capacity after long-term application of manure and chemical fertilizer, as well as to properly manage soil fertility through fertilizer application under the soil-climatic conditions of the North China Plain, organic N forms were quantified in the topsoil with different manure and chemical fertilizer treatments in a 15-year fertilizer experiment in a Chinese calcareous alluvial soil. Soil total N (TN) and various organic N forms were significantly influenced by long-term application of chemical fertilizer and manure. TN, total hydrolysable N, acid-lnsoluble N, amino acid N and ammonium N in the soil increased significantly (P 〈 0.05) with increasing manure and fertilizer N rates, but were not influenced by increasing P rates. Also, application of manure or N fertilizer or P fertilizer did not significantly influence either the quantity of amino sugar N or its proportion of TN. Application of manure significantly increased (P 〈 0.05) hydrolysable unknown N, but adding N or P did not. In addition, application of manure or N fertilizer or P fertilizer did not significantly influence the proportions of different soil organic N forms.
基金supported by the grants of the National Natural Science Foundation of China(40901147, 31070399, and 41071171)the International Cooperation Project of Heilongjiang Province,China (WB09B105)
文摘Human activities have altered weather patterns by causing an increase in greenhouse gas. The effects of climate change have been studied, including effects on some ecosystems throughout the world. There are many studies on changes in the soil due to climate change, but much of them did not extend their research to soil enzyme that integrates information on soil microbial status and soil physical-chemical conditions. Meanwhile, there are lots of experimental fields established to study effects of long-term fertilization on soil enzyme activities, but many did not compare the difference of soil enzyme activities and did not analyze the effect of climatic factors on soil enzyme activities with long-term fertilization under different hydrothermal conditions. In this study, we compared soil enzyme activities of three long-fertilization stations which had different hydrothermal conditions in Northeast China, and analyzed the relationship of hydrothermal condition, soil chemical properties with soil enzyme activities. Hydrothermal conditions (annual temperature and total rainfall) decreased in order of Gongzhuling (Jilin Province, China ) Harbin (Heilongjiang Province, China) Heihe (Heilongjiang Province, China) over the course of the long-term fertilization experiment. Sunshine hours showed the longest in Gongzhuling, the second in Heihe, and the last in Harbin. However, the order of soil enzymes was not in agreement with hydrothermal conditions. Overall, the order of soil enzymes for the same treatment among three stations was consistent in 2008 with in 2009. Correlation analysis demonstrated that different soil enzymes achieved the different affected levels by climatic factors under different fertilization treatments. Urease activity showed a significant relationship with sunshine hours in no fertilizer (CK) treatment (R=-0.91, P0.01) and relative humidity in mineral fertilizers plus manure (MNPK) treatment (R=0.82, P0.05). Phosphatase activity exhibited a negative correlation with annual mean temperature, annual mean maximum temperature and annual mean minimum temperature, and their correlation coefficients were separately -0.83, -0.79, and -0.83 at P0.05 in CK treatment. Invertase activity was highly and positively correlated with sunshine hours in CK treatment (R=0.94, P0.01). Catalase activity showed significant negative correlations with minimum relative humidity in CK treatment (R=-0.81, P0.05), and positive correlations with sunshine hours in M treatment (R=0.83, P0.05). There were no climatic factors which strongly affected on dehydrogenase in all treatments. Soil enzyme activities were closely related to the soil chemical properties. Soil urease activity was positively correlated with available P (P0.05). With exception of correlation between invertase and total P at P0.05, phosphase, invertase, catalase, and dehydrogenase showed significant positive correlations with soil chemical properties (P0.01). It was a comprehensive process that biologic and abiotic factors were effect on soil enzyme activities under different fertilization treatments. To sum up, the variation of hydrothermal conditions in different climate zones and soil chemical properties affect integrally metabolic activity and metabolic finger print of microbial communities in black soil.
基金supported by the National Natural Science Foundation of China (41330744)the “973” Program of China (2014CB953803)the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (164320H116)
文摘Application of fertilizer has been found to significantly affect soil N cycling. However, a comprehensive understanding of the effects of long-term fertilization on soil gross N transformation rates is still lacking. We compiled data of observations from 10 long-term fertilization experiments and conducted a meta-analysis of the effects of long-term fertilization on soil gross N transformation rates. The results showed that if chemical fertilizers of N, P and K were applied in balance, soil p H decreased very slightly. There was a significantly positive effect of long-term fertilization, either chemical or organic fertilizers or their combinations, on gross N mineralization rate compared to the control treatment(the mean effect size ranged from 1.21 to 1.25 at 95% confidence intervals(CI) with a mean of 1.23), mainly due to the increasing soil total N content. The long-term application of organic fertilizer alone and combining organic and chemical fertilizer could increase the mineralization-immobilization turnover, thus enhance available N for plant while reduce N losses potential compared to the control treatment. However, long-term chemical fertilizer application did not significantly affect the gross NH4+ immobilization rate, but accelerated gross nitrification rate(1.19; 95% CI: 1.08 to 1.31). Thus, long-term chemical fertilizer alone would probably induce higher N losses potential through NO3– leaching and runoff than organic fertilizer application compared to the control treatment. Therefore, in the view of the effects of long-term fertilization on gross N transformation rates, it also supports that organic fertilizer alone or combination of organic and chemical fertilizer could not only improve crop yield, but also increase soil fertility and reduce the N losses potential.
文摘为了研究化肥减量配施有机肥对木薯根际土壤细菌多样性和群落结构及产量的影响,采用Illumina Nova 6000测序平台,对未施肥(CK)、有机肥(T_(1))、常规施肥+有机肥(T_(2))和化肥减施+有机肥(T_(3))等施肥方式处理下的华南12号木薯根际土壤进行16S rRNA高通量测序分析。结果表明,与CK相比,T_(3)处理不仅能显著增加木薯株高和茎粗,还能提高木薯产量。在相似水平为97%下聚类分析得到木薯根际土壤OTUs,分别为3603个(CK)、1688个(T_(1))、1276个(T_(2))和3317个(T_(3))。不同施肥方式改变土壤细菌的多样性和丰度,其物种多样性由高到低依次为:CK>T_(3)>T_(1)>T_(2)。基于土壤细菌群落的PCoA和聚类分析,CK和T_(3)细菌群落结构组成较为相似,T_(1)与T_(2)细菌群落结构组成相似。不同施肥处理下木薯根际土壤中优势菌门为变形菌门、拟杆菌门、酸杆菌门、厚壁菌门、放线菌门和绿弯菌门。化肥减施配施有机肥增加了变形菌门、拟杆菌门和放线菌门的相对丰度,降低了厚壁菌门和绿弯菌门的相对丰度。冗余性分析结果表明速效钾是影响木薯根际土壤菌群的主要效应因子。研究表明,化肥减量配施有机肥不仅能增加木薯的产量,还能改变木薯根际土壤细菌群落结构和多样性,为发展绿色、高效、可持续的木薯产业提供坚实的理论支撑。