The effects of ionomycin, electrical pulses, dithiothreitol(DTT), 6-DMAP and thimersal, used either alone or in combinations, on the activation rate, pronuclear type and cleavage of porcine oocyte were studied in this...The effects of ionomycin, electrical pulses, dithiothreitol(DTT), 6-DMAP and thimersal, used either alone or in combinations, on the activation rate, pronuclear type and cleavage of porcine oocyte were studied in this paper. The results are as follows:(1)The activation rates of oocytes treated with ionomycin(88.9%)or pulsed(80.0%)alone did not differ significantly from each other and from those in oocytes treated in combination with 6-DMAP(84. 3 and 79. 1%, respectively). While 6-DMAP improved electrical activation(increased the proportion of oocytes with 1 PN), it had no effect on oocyte activation by ionomycin;(2)Post-treatment with DTT significantly enhanced the thimersal activation of porcine oocytes with activation rates increased from 4.6 to 82.6%;(3)When pulsed, the activation rates of oocytes with crimped(85.4%)or fragmented first polar bodies(86. 2%)were significantly higher than those of oocytes with intact polar bodies(58. 8%); the ratio of 1 PN activated oocytes(78. 8%)was significantly higher, but that of 2 PN oocytes(18. 2%)was significantly lower in oocytes with intact polar bodies;(4)The cleavage rate of oocytes activated by electrical stimulus(52.4%)was significantly higher than that in oocytes activated by ionomycin(23.0%)when combined with 6-DMAP, but it was not significantly different from that in oocytes activated by electrical stimulus alone(46.1%).展开更多
基金supported by a grant from the 973 Program of Science and Technology Ministry,China(G200016107).
文摘The effects of ionomycin, electrical pulses, dithiothreitol(DTT), 6-DMAP and thimersal, used either alone or in combinations, on the activation rate, pronuclear type and cleavage of porcine oocyte were studied in this paper. The results are as follows:(1)The activation rates of oocytes treated with ionomycin(88.9%)or pulsed(80.0%)alone did not differ significantly from each other and from those in oocytes treated in combination with 6-DMAP(84. 3 and 79. 1%, respectively). While 6-DMAP improved electrical activation(increased the proportion of oocytes with 1 PN), it had no effect on oocyte activation by ionomycin;(2)Post-treatment with DTT significantly enhanced the thimersal activation of porcine oocytes with activation rates increased from 4.6 to 82.6%;(3)When pulsed, the activation rates of oocytes with crimped(85.4%)or fragmented first polar bodies(86. 2%)were significantly higher than those of oocytes with intact polar bodies(58. 8%); the ratio of 1 PN activated oocytes(78. 8%)was significantly higher, but that of 2 PN oocytes(18. 2%)was significantly lower in oocytes with intact polar bodies;(4)The cleavage rate of oocytes activated by electrical stimulus(52.4%)was significantly higher than that in oocytes activated by ionomycin(23.0%)when combined with 6-DMAP, but it was not significantly different from that in oocytes activated by electrical stimulus alone(46.1%).
