Optimization of Microwave-assisted Extraction Technology of Polyphenols from Loropetalum chinense(R. Br.) Oliv

In order to optimize the microwave-assisted extraction technology of polyphenols from Loropetalum chinense （R. Br.） Oliv., the effects of microwave power, ethanol concentration, solid to liquid ratio and extraction time on polyphenols extraction rate were investigated. On the basis of single-factor test, a four-factor and three-level orthogonal test was designed by response surface method to establish a mathematical model between the response value and various factors. The results showed that the intensity of effect of different influencing factor on polyphenols ex- traction rate ranked as microwave power＇s〉solid to liquid ratio＇s〉extraction time＇s〉 ethanol concentration＇s. The optimum microwave-assisted extraction conditions for polyphenols from L. chinense were as follows： extraction power 254 W, ethanol concentration 60%, extraction time 12.5 rain and solid to liquid ratio 1：17. Under the optimum extraction conditions, the extraction rate of polyphenols from L. chinense was 19.17%.

红花檵木全基因组分析

红花檵木花红叶艳、耐修剪、抗逆性强和生态适应性广,广泛应用于城市绿化和园林美化,是重要的中药材。其分子生物研究进展缓慢,红花檵木全基因组的破译能为其花色、叶色、株型、主要功能成分代谢等方面的深入研究提供坚实的基础。本研究基于Illumina测序平台测定了红花檵木基因组,运用生物信息学的方法预测其基因组大小,分析了基因组的杂合度、重复序列情况、GC含量等基因组特征,并进一步用SOAP de novo软件进行了基因组的组装。研究表明:红花檵木基因组大小的校正值为2.87 Gb;红花檵木基因组具有高重复序列和低杂合的特点,重复序列比率为76.32%,杂合率为0.20%,GC含量为35.52%;利用SOAP de novo软件对49.42 Gb Clean data进行了组装,得到4 333 224条Scaffold,拼接序列总长度为1 504 451 854 bp,组装后得到的最长序列为26 254 bp,N50为528 bp,N90为133 bp,后续研究建议采用Illumina+PacBio RSⅡ测序技术相结合方式,辅以Hi-C技术及其相应的拼接组装软件,有望得较好的红花檵木全基因组图谱。