AIM To elucidate tongue coating microbiota and metabolic differences in chronic hepatitis B(CHB) patients with yellow or white tongue coatings.METHODS Tongue coating samples were collected from 53 CHBpatients(28 CHB y...AIM To elucidate tongue coating microbiota and metabolic differences in chronic hepatitis B(CHB) patients with yellow or white tongue coatings.METHODS Tongue coating samples were collected from 53 CHBpatients(28 CHB yellow tongue coating patients and 25 CHB white tongue coating patients) and 22 healthy controls.Microbial DNA was extracted from the tongue samples,and the bacterial 16 S ribosomal RNA gene V3 region was amplified from all samples and sequenced with the Ion Torrent PGM^(TM)sequencing platform according to the standard protocols.The metabolites in the tongue coatings were evaluated using a liquid chromatographymass spectrometry(LC-MS) platform.Statistical analyses were then performed.RESULTS The relative compositions of the tongue coating microbiotas and metabolites in the CHB patients were significantly different from those of the healthy controls,but the tongue coating microbiota abundances and diversity levels were not significantly different.Compared with the CHB white tongue coating patients,the CHB yellow tongue coating patients had higher hepatitis B viral DNA(HBV-DNA) titers(median 21210 vs 500,respectively,P = 0.03) and a significantly lower level of Bacteroidetes(20.14% vs 27.93%,respectively,P = 0.013) and higher level of Proteobacteria(25.99% vs 18.17%,respectively,P = 0.045) in the microbial compositions at the phylum level.The inferred metagenomic pathways enriched in the CHB yellow tongue coating patients were mainly those involved in amino acid metabolism,which was consistent with the metabolic disorder.The abundances of bacteria from Bacteroidales at the order level were higher in the CHB white tongue coating patients(19.2% vs 27.22%,respectively,P = 0.011),whereas Neisseriales were enriched in the yellow tongue coating patients(21.85% vs 13.83%,respectively,P = 0.029).At the family level,the abundance of Neisseriaceae in the yellow tongue patients was positively correlated with the HBV-DNA level but negatively correlated with the S-adenosyl-L-methionine level.CONCLUSION This research illustrates specific clinical features and bacterial structures in CHB patients with different tongue coatings,which facilitates understanding of the traditional tongue diagnosis.展开更多
<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atr...<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atrophic gastritis patients and 29 age and gender-matched non-atrophic gastritis controls. By sequencing the V3-V4 region of the 16S rRNA, we investigated the microbial community structure and diversity on the tongue coating. <b>Results:</b> There was no significant difference in the microbial diversity on the tongue coating between the two groups. However, compared with the control, the atrophic gastritis group had a smaller number of operational taxonomic units (OTUs). At the class level, patients with atrophic gastritis had lower relative abundances of <i>Betaproteobacteria</i> and <i>Spirochaetia</i> than the control group. At the gene level, the abundance of <i>Neisseria</i> and <i>Aggregatibacter</i> in atrophic gastritis group had significantly decreased compared with control ones. Furthermore, functional prediction revealed that 24 metabolic pathways significantly differed between the two groups. <b>Conclusions:</b> Our findings provide novel evidence that tongue coating microbiota may be a biomarker for characterizing patient with atrophic gastritis, but its mechanism needs to be further elaborated.展开更多
In this work we study two types of Discrete Hill’s equation. The first comes from the discretization process of a Continuous-time Hill’s equation, we called Discretized Hill’s equation. The Second is a naturally ob...In this work we study two types of Discrete Hill’s equation. The first comes from the discretization process of a Continuous-time Hill’s equation, we called Discretized Hill’s equation. The Second is a naturally obtained in Discrete-Time and will be called Discrete-time Hill’s equation. The objective of discretization is preserving the continuous-time behavior and we show this property. On the contrary a completely different dynamic property was found for the Discrete-Time Hill’s equation. At the end of the paper is shown that both types share the nonoscillatory behavior of solutions in the 0-th Arnold Tongue.展开更多
OBJECTIVE:To explore the correlation between tongue and oral microbiota,we studied the microbial community structure of different tongue coating types in patients with chronic gastritis.METHODS:16S rDNA gene sequencin...OBJECTIVE:To explore the correlation between tongue and oral microbiota,we studied the microbial community structure of different tongue coating types in patients with chronic gastritis.METHODS:16S rDNA gene sequencing and bioinformatics analysis were used to study the dynamic changes and correlation of microbial flora in patients with chronic gastritis,healthy people,and patients with different tongue fur.In addition,it was also discussed between the severity of gastritis and the microflora of tongue fur.RESULTS:The microbial diversity of tongue fur in patients with chronic gastritis was significantly different from healthy controls.There were significant changes in bacterial communities’diversity and relative abundance between extra tongue fur in patients but not in healthy people.Oral bacteria with relative abundance>1%and P<0.05 among different tongue fur flora were dominant bacteria,including 12 phyla such as Bacteroidetes,Proteobacteria and Firmicutes,and 256 genera such as Neisseria,Prevotella_7 and Haemophilus.CONCLUSIONS:The changes in oral flora in patients with chronic gastritis were related to tongue fur.Therefore,the significant microbiota might enlighten further study on the correlation between tongue inspection and oral microbiota in patients with chronic gastritis.展开更多
BACKGROUND Primary intestinal lymphangiectasia(PIL),first described in 1961,is a rare disorder of unknown etiology resulting in protein-losing enteropathy.The disease is characterized by dilatation and leakage of inte...BACKGROUND Primary intestinal lymphangiectasia(PIL),first described in 1961,is a rare disorder of unknown etiology resulting in protein-losing enteropathy.The disease is characterized by dilatation and leakage of intestinal lymph vessels leading to hypoalbuminemia,hypogammaglobulinemia,and lymphopenia.Since the severity and location of lymph vessels being affected can vary considerably,the range of associated symptoms is wide from mild lower-limb edema to generalized edema,abdominal and/or pleural effusion,and recurrent diarrhea,among others.Although usually developing in early childhood,we present the case of a 34-yearold woman with PIL.Moreover,we performed a literature review systematically assessing clinical presentation,and provide a practical approach to facilitate diagnosis and therapy of PIL in adults.CASE SUMMARY Our patient presented with unspecific symptoms of abdominal discomfort,fatigue,nausea,and recurrent edema of the lower limbs.Interestingly,a striking collinearity of clinical symptoms with female hormone status was evident.Additionally,polyglobulia,hypoalbuminemia,hypogammaglobulinemia,and transient lymphocytopenia were evident.Due to suspicion of a bone marrow disease,an extensive diagnostic investigation was carried out excluding secondary causes of polyglobulinemia and hypoalbuminemia.The diagnosis of primary intestinal lymphangiectasia was established after 22 wk by histological analysis of biopsy samples obtained via enteroscopy.Consecutively,the patient was put on a high-protein and low-fat diet with medium-chain triglycerides supplementation leading to significant improvement of clinical symptoms until 2 years of follow-up.CONCLUSION PIL can be the reason for cryptogenic hypoalbuminemia,hypogammaglobulinemia,and lymphopenia in adulthood.Due to difficulty in correct diagnosis,treatment initiation is often delayed despite being effective and welltolerated.This leads to a significant disease burden in affected patients.PIL is increasingly been recognized in adults since the majority of case reports were published within the last 10 years,pointing towards an underestimation of the true prevalence.The association with female hormone status warrants further investigation.展开更多
In China, hot researches on shale oil were raised by the important breakthrough of shale oil in America. Obviously, the first important issue is the actual shale oil resource potential of China, and the selection of t...In China, hot researches on shale oil were raised by the important breakthrough of shale oil in America. Obviously, the first important issue is the actual shale oil resource potential of China, and the selection of the key appraisement parameter is vital to the shale oil resource amount. Among the appraisement parameters, the oil content parameter(S1) is the key one, but the evaluation result is generally lower because of light hydrocarbon losing and heavy hydrocarbon handling. And the more important thing is that the light hydrocarbon with small molecular weight is more recoverable, and therefore its amount is important to the total shale oil yields. Based on pyrolysis experiments and the kinetic model of hydrocarbon generation, correction factors and a model of light hydrocarbon losing and heavy hydrocarbon handling were established. The results show that the correction factor of heavy hydrocarbon handling is 3.2, and that of light hydrocarbon losing is controlled by kerogen type, maturity and hydrocarbon generation environment(closed or open).展开更多
Based on the researches of language transfer,contrastive analysis and error analysis,this paper tries to investigate the negative transfer of Chinese on Chinese students'English writings from the perspective of sy...Based on the researches of language transfer,contrastive analysis and error analysis,this paper tries to investigate the negative transfer of Chinese on Chinese students'English writings from the perspective of syntax.During one and a half months'teaching practice in Shannxi Hanzhong No.1 Middle School,the author lived through the real teaching process and found that there were lots of problems in students'English writings.The author makes a contrastive study of the different characteristics existing in Chinese and English languag es at the level of syntax,and analyses the typical syntactic errors appearing in the 60 students'English compositions collected from one or dinary class.These syntactic errors are caused by the negative transfer of Chinese.The purpose of the investigation is to study in what pat tern students have been influenced by Chinese in their English writings.Based on the findings and analysis of the mother tongue transfer on English writings,some implications are also provided in order to help minimize or eliminate the interference of negative transfer of the Chinese in students'English writings and improve the teaching and learning of English.展开更多
Whilst ascites is a common presenting complaint in patients with decompensated chronic liver disease and disseminated malignancy,in Crohn's disease however,it is exceptionally rare.We describe a patient with no pr...Whilst ascites is a common presenting complaint in patients with decompensated chronic liver disease and disseminated malignancy,in Crohn's disease however,it is exceptionally rare.We describe a patient with no prior history of inflammatory bowel or liver disease,presenting with rapid onset gross ascites and scrotal swelling.Further investigations revealed severe hypoalbuminemia and transudative ascitic fluid with normal other liver function tests and a negative liver screen.Computed tomography revealed widespread ascites and pleural effusions with no features of malignancy or portal hypertension,and a small bowel barium series showed features of fistulating small bowel Crohn's disease.An ileo-colonoscopy confirmed the presence of terminal ileal inflammatory stricture.The patient's clinical condition and serum albumin improved with a combination of diuretics,elemental diet,antibiotics and oral 5-aminosalicylic acid therapy.展开更多
In this article,one proved case of flaccid tongue treated by Guan’s tongue acupuncture was introduced by the three-step method of"puncture,prick,and swallowing".This case was an elderly female patient with ...In this article,one proved case of flaccid tongue treated by Guan’s tongue acupuncture was introduced by the three-step method of"puncture,prick,and swallowing".This case was an elderly female patient with Parkinson disease,which had the main clinical symptoms of flaccid and weak tongue,and speech and swallowing disorder,and was diagnosed as flaccid tongue(deficiency of the both qi and blood,and deficiency syndrome of liver and kidney).The patient received diagnosis and treatment based on an overall analysis of its dialectical characteristics,using the five acupoints of Heart,Jīnjīn(金津EX-HN12),Yùyè(玉液EX-HN13),Tongue root acupoint 1,and Tongue root acupoint 2 in total.First,the four acupoints of EX-HN12,EX-HN13,Tongue root acupoint 1,and Tongue root acupoint 2 were punctured successively,and then using the reinforcing method,the Heart on the tongue surface was pricked.Finally,the patient was advised to make a slow swallowing action.Through tongue acupuncture therapy for 6 times,the patient’s speech and swallowing function was improved,which achieved a satisfactory therapeutic effects.展开更多
基金Supported by the Shanghai Educational Development Foundation,No.14CG41the National Natural Science Foundation of China,No.81403298 and No.81373857the National Key New Drug Creation Project,No.2017ZX09304002
文摘AIM To elucidate tongue coating microbiota and metabolic differences in chronic hepatitis B(CHB) patients with yellow or white tongue coatings.METHODS Tongue coating samples were collected from 53 CHBpatients(28 CHB yellow tongue coating patients and 25 CHB white tongue coating patients) and 22 healthy controls.Microbial DNA was extracted from the tongue samples,and the bacterial 16 S ribosomal RNA gene V3 region was amplified from all samples and sequenced with the Ion Torrent PGM^(TM)sequencing platform according to the standard protocols.The metabolites in the tongue coatings were evaluated using a liquid chromatographymass spectrometry(LC-MS) platform.Statistical analyses were then performed.RESULTS The relative compositions of the tongue coating microbiotas and metabolites in the CHB patients were significantly different from those of the healthy controls,but the tongue coating microbiota abundances and diversity levels were not significantly different.Compared with the CHB white tongue coating patients,the CHB yellow tongue coating patients had higher hepatitis B viral DNA(HBV-DNA) titers(median 21210 vs 500,respectively,P = 0.03) and a significantly lower level of Bacteroidetes(20.14% vs 27.93%,respectively,P = 0.013) and higher level of Proteobacteria(25.99% vs 18.17%,respectively,P = 0.045) in the microbial compositions at the phylum level.The inferred metagenomic pathways enriched in the CHB yellow tongue coating patients were mainly those involved in amino acid metabolism,which was consistent with the metabolic disorder.The abundances of bacteria from Bacteroidales at the order level were higher in the CHB white tongue coating patients(19.2% vs 27.22%,respectively,P = 0.011),whereas Neisseriales were enriched in the yellow tongue coating patients(21.85% vs 13.83%,respectively,P = 0.029).At the family level,the abundance of Neisseriaceae in the yellow tongue patients was positively correlated with the HBV-DNA level but negatively correlated with the S-adenosyl-L-methionine level.CONCLUSION This research illustrates specific clinical features and bacterial structures in CHB patients with different tongue coatings,which facilitates understanding of the traditional tongue diagnosis.
文摘<b>Aims:</b> To explore the tongue coating microbiota composition in patients with atrophic gastritis by using the 16S rRNA gene sequencing technology. <b>Methods:</b> The study included 29 atrophic gastritis patients and 29 age and gender-matched non-atrophic gastritis controls. By sequencing the V3-V4 region of the 16S rRNA, we investigated the microbial community structure and diversity on the tongue coating. <b>Results:</b> There was no significant difference in the microbial diversity on the tongue coating between the two groups. However, compared with the control, the atrophic gastritis group had a smaller number of operational taxonomic units (OTUs). At the class level, patients with atrophic gastritis had lower relative abundances of <i>Betaproteobacteria</i> and <i>Spirochaetia</i> than the control group. At the gene level, the abundance of <i>Neisseria</i> and <i>Aggregatibacter</i> in atrophic gastritis group had significantly decreased compared with control ones. Furthermore, functional prediction revealed that 24 metabolic pathways significantly differed between the two groups. <b>Conclusions:</b> Our findings provide novel evidence that tongue coating microbiota may be a biomarker for characterizing patient with atrophic gastritis, but its mechanism needs to be further elaborated.
文摘In this work we study two types of Discrete Hill’s equation. The first comes from the discretization process of a Continuous-time Hill’s equation, we called Discretized Hill’s equation. The Second is a naturally obtained in Discrete-Time and will be called Discrete-time Hill’s equation. The objective of discretization is preserving the continuous-time behavior and we show this property. On the contrary a completely different dynamic property was found for the Discrete-Time Hill’s equation. At the end of the paper is shown that both types share the nonoscillatory behavior of solutions in the 0-th Arnold Tongue.
基金Supported by National Natural Science Foundation of China:Study on the Mechanism of New Sophora Japonica Extract Inhibiting the Formation of Liver Pre Metastasis Microenvironment by Down Regulating the Gastric Cancer Cell-Derived Exosome ITGB5(No.81973634)Key Research And Development Plan of Anhui Province:Development And Application of TCM Health Status Identification System Based on Artificial Intelligence Tongue Diagnosis(No.2022h11020018)Key Projects of Anhui Provincial Department of Education:Research on Key Technologies of Artificial Intelligence Tongue Color Recognition Based on Machine Learning(No.KJ2020A0379)。
文摘OBJECTIVE:To explore the correlation between tongue and oral microbiota,we studied the microbial community structure of different tongue coating types in patients with chronic gastritis.METHODS:16S rDNA gene sequencing and bioinformatics analysis were used to study the dynamic changes and correlation of microbial flora in patients with chronic gastritis,healthy people,and patients with different tongue fur.In addition,it was also discussed between the severity of gastritis and the microflora of tongue fur.RESULTS:The microbial diversity of tongue fur in patients with chronic gastritis was significantly different from healthy controls.There were significant changes in bacterial communities’diversity and relative abundance between extra tongue fur in patients but not in healthy people.Oral bacteria with relative abundance>1%and P<0.05 among different tongue fur flora were dominant bacteria,including 12 phyla such as Bacteroidetes,Proteobacteria and Firmicutes,and 256 genera such as Neisseria,Prevotella_7 and Haemophilus.CONCLUSIONS:The changes in oral flora in patients with chronic gastritis were related to tongue fur.Therefore,the significant microbiota might enlighten further study on the correlation between tongue inspection and oral microbiota in patients with chronic gastritis.
文摘BACKGROUND Primary intestinal lymphangiectasia(PIL),first described in 1961,is a rare disorder of unknown etiology resulting in protein-losing enteropathy.The disease is characterized by dilatation and leakage of intestinal lymph vessels leading to hypoalbuminemia,hypogammaglobulinemia,and lymphopenia.Since the severity and location of lymph vessels being affected can vary considerably,the range of associated symptoms is wide from mild lower-limb edema to generalized edema,abdominal and/or pleural effusion,and recurrent diarrhea,among others.Although usually developing in early childhood,we present the case of a 34-yearold woman with PIL.Moreover,we performed a literature review systematically assessing clinical presentation,and provide a practical approach to facilitate diagnosis and therapy of PIL in adults.CASE SUMMARY Our patient presented with unspecific symptoms of abdominal discomfort,fatigue,nausea,and recurrent edema of the lower limbs.Interestingly,a striking collinearity of clinical symptoms with female hormone status was evident.Additionally,polyglobulia,hypoalbuminemia,hypogammaglobulinemia,and transient lymphocytopenia were evident.Due to suspicion of a bone marrow disease,an extensive diagnostic investigation was carried out excluding secondary causes of polyglobulinemia and hypoalbuminemia.The diagnosis of primary intestinal lymphangiectasia was established after 22 wk by histological analysis of biopsy samples obtained via enteroscopy.Consecutively,the patient was put on a high-protein and low-fat diet with medium-chain triglycerides supplementation leading to significant improvement of clinical symptoms until 2 years of follow-up.CONCLUSION PIL can be the reason for cryptogenic hypoalbuminemia,hypogammaglobulinemia,and lymphopenia in adulthood.Due to difficulty in correct diagnosis,treatment initiation is often delayed despite being effective and welltolerated.This leads to a significant disease burden in affected patients.PIL is increasingly been recognized in adults since the majority of case reports were published within the last 10 years,pointing towards an underestimation of the true prevalence.The association with female hormone status warrants further investigation.
基金supported by "the Fundamental Research Funds for the Central Universities" (14CX05017A)the Natural Science Foundation of China (41330313)+1 种基金Program for New Century Excellent Talents in Heilongjiang Provincial University (1252-NCET-012)CNPC Innovation Foundation (2011D-5006-0101)
文摘In China, hot researches on shale oil were raised by the important breakthrough of shale oil in America. Obviously, the first important issue is the actual shale oil resource potential of China, and the selection of the key appraisement parameter is vital to the shale oil resource amount. Among the appraisement parameters, the oil content parameter(S1) is the key one, but the evaluation result is generally lower because of light hydrocarbon losing and heavy hydrocarbon handling. And the more important thing is that the light hydrocarbon with small molecular weight is more recoverable, and therefore its amount is important to the total shale oil yields. Based on pyrolysis experiments and the kinetic model of hydrocarbon generation, correction factors and a model of light hydrocarbon losing and heavy hydrocarbon handling were established. The results show that the correction factor of heavy hydrocarbon handling is 3.2, and that of light hydrocarbon losing is controlled by kerogen type, maturity and hydrocarbon generation environment(closed or open).
文摘Based on the researches of language transfer,contrastive analysis and error analysis,this paper tries to investigate the negative transfer of Chinese on Chinese students'English writings from the perspective of syntax.During one and a half months'teaching practice in Shannxi Hanzhong No.1 Middle School,the author lived through the real teaching process and found that there were lots of problems in students'English writings.The author makes a contrastive study of the different characteristics existing in Chinese and English languag es at the level of syntax,and analyses the typical syntactic errors appearing in the 60 students'English compositions collected from one or dinary class.These syntactic errors are caused by the negative transfer of Chinese.The purpose of the investigation is to study in what pat tern students have been influenced by Chinese in their English writings.Based on the findings and analysis of the mother tongue transfer on English writings,some implications are also provided in order to help minimize or eliminate the interference of negative transfer of the Chinese in students'English writings and improve the teaching and learning of English.
文摘Whilst ascites is a common presenting complaint in patients with decompensated chronic liver disease and disseminated malignancy,in Crohn's disease however,it is exceptionally rare.We describe a patient with no prior history of inflammatory bowel or liver disease,presenting with rapid onset gross ascites and scrotal swelling.Further investigations revealed severe hypoalbuminemia and transudative ascitic fluid with normal other liver function tests and a negative liver screen.Computed tomography revealed widespread ascites and pleural effusions with no features of malignancy or portal hypertension,and a small bowel barium series showed features of fistulating small bowel Crohn's disease.An ileo-colonoscopy confirmed the presence of terminal ileal inflammatory stricture.The patient's clinical condition and serum albumin improved with a combination of diuretics,elemental diet,antibiotics and oral 5-aminosalicylic acid therapy.
文摘In this article,one proved case of flaccid tongue treated by Guan’s tongue acupuncture was introduced by the three-step method of"puncture,prick,and swallowing".This case was an elderly female patient with Parkinson disease,which had the main clinical symptoms of flaccid and weak tongue,and speech and swallowing disorder,and was diagnosed as flaccid tongue(deficiency of the both qi and blood,and deficiency syndrome of liver and kidney).The patient received diagnosis and treatment based on an overall analysis of its dialectical characteristics,using the five acupoints of Heart,Jīnjīn(金津EX-HN12),Yùyè(玉液EX-HN13),Tongue root acupoint 1,and Tongue root acupoint 2 in total.First,the four acupoints of EX-HN12,EX-HN13,Tongue root acupoint 1,and Tongue root acupoint 2 were punctured successively,and then using the reinforcing method,the Heart on the tongue surface was pricked.Finally,the patient was advised to make a slow swallowing action.Through tongue acupuncture therapy for 6 times,the patient’s speech and swallowing function was improved,which achieved a satisfactory therapeutic effects.