Lotus root polysaccharide inhibits the growth of ovarian cancer cells by blocking the cell cycle

Background:Lotus root polysaccharide is a natural antioxidant.As a new anticancer drug,it has anti-proliferation and pro-apoptotic effects in a variety of tumour cells,but its effect on ovarian cancer is not clear.In study,we attempted to elucidate the role and mechanism of lotus root polysaccharide in SKOV3 cells.Methods:In this study,the effect of lotus root polysaccharide on mRNA of SKVO3 cells was analyzed by RNA-seq,and verified by Western blot,flow cytometry,fluorescence detection and other techniques.Results:The results showed that lotus root polysaccharide could inhibit the proliferation of ovarian cancer cells.Then,a change in gene expression was found by RNA-seq.In the mRNA(differentially expressed mRNA)with these differences,significant changes in the cell cycle were found by Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis.Subsequently,the proportion of cells in S phase decreases and G2/M phase increases,as seen with propidium iodide staining.Gene Set Enrichment Analysis showed inhibition of the cell cycle,and the gene and protein expression of CDK1,CCNA1 and CCNB1 were inhibited.Conclusion:Our results show that lotus root polysaccharide can inhibit the growth of SKOV3 cells in vitro by blocking the cell cycle at the G2/M phase,which reveals the potential of lotus root polysaccharide in the treatment of ovarian cancer.