Background Low crude protein(CP)formulations with supplemental amino acids(AA)are used to enhance intestinal health,reduce costs,minimize environmental impact,and maintain growth performance of pigs.However,extensive ...Background Low crude protein(CP)formulations with supplemental amino acids(AA)are used to enhance intestinal health,reduce costs,minimize environmental impact,and maintain growth performance of pigs.However,extensive reduction of dietary CP can compromise growth performance due to limited synthesis of non-essential AA and limited availability of bioactive compounds from protein supplements even when AA requirements are met.Moreover,implementing a low CP formulation can increase the net energy(NE)content in feeds causing excessive fat deposition.Additional supplementation of functional AA,coupled with low CP formulation could further enhance intestinal health and glucose metabolism,improving nitrogen utilization,and growth performance.Three experiments were conducted to evaluate the effects of low CP formulations with supplemental AA on the intestinal health and growth performance of growing-finishing pigs.Methods In Exp.1,90 pigs(19.7±1.1 kg,45 barrows and 45 gilts)were assigned to 3 treatments:CON(18.0%CP,supplementing Lys,Met,and Thr),LCP(16.0%CP,supplementing Lys,Met,Thr,Trp,and Val),and LCPT(16.1%CP,LCP+0.05%SID Trp).In Exp.2,72 pigs(34.2±4.2 kg BW)were assigned to 3 treatments:CON(17.7%CP,meeting the requirements of Lys,Met,Thr,and Trp);LCP(15.0%CP,meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and VLCP(12.8%CP,meeting Lys,Thr,Trp,Met,Val,Ile,Phe,His,and Leu).In Exp.3,72 pigs(54.1±5.9 kg BW)were assigned to 3 treatments and fed experimental diets for 3 phases(grower 2,finishing 1,and finishing 2).Treatments were CON(18.0%,13.8%,12.7%CP for 3 phases;meeting Lys,Met,Thr,and Trp);LCP(13.5%,11.4%,10.4%CP for 3 phases;meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and LCPG(14.1%,12.8%,11.1%CP for 3 phases;LCP+Glu to match SID Glu with CON).All diets had 2.6 Mcal/kg NE.Results In Exp.1,overall,the growth performance did not differ among treatments.The LCPT increased(P<0.05)Claudin-1 expression in the duodenum and jejunum.The LCP and LCPT increased(P<0.05)CAT-1,4F2hc,and B0AT expressions in the jejunum.In Exp.2,overall,the VLCP reduced(P<0.05)G:F and BUN.The LCP and VLCP increased(P<0.05)the backfat thickness(BFT).In Exp.3,overall,growth performance and BFT did not differ among treatments.The LCPG reduced(P<0.05)BUN,whereas increased the insulin in plasma.The LCP and LCPG reduced(P<0.05)the abundance of Streptococcaceae,whereas the LCP reduced(P<0.05)Erysipelotrichaceae,and the alpha diversity.Conclusions When implementing low CP formulation,CP can be reduced by supplementation of Lys,Thr,Met,Trp,Val,and Ile without affecting the growth performance of growing-finishing pigs when NE is adjusted to avoid increased fat deposition.Supplementation of Trp above the requirement or supplementation of Glu in low CP formulation seems to benefit intestinal health as well as improved nitrogen utilization and glucose metabolism.展开更多
[ Objectives ] To study the effects of low-protein diets supplemented with amino acids on the production performance, carcass quality and input-output ratio of fattening pigs and growing-finishing pigs. [ Methods] Two...[ Objectives ] To study the effects of low-protein diets supplemented with amino acids on the production performance, carcass quality and input-output ratio of fattening pigs and growing-finishing pigs. [ Methods] Two studies were conducted on fattening pigs and growing pigs respectively. Pigs in two experimental groupswere fed with low-protein diets supplemented with amino acids and normal diets respectively, and the initial weight, final weight, feed consumption and car- cass index of the tested pigs were measured. [ Results] The average final weight of fattening pigs in test group was 2.4 kg lower than that in control group, while the difference was not significant (P 〉 0.05 ). The average daily gain of fattening pigs in test group was 60 g lower than that in control group, indicating that low protein diets had a tendency to decrease the growth rate (P 〉 0.05 ), and reduction of daily weight gain in low protein diet pigs reached 7.6%. The development of pigs in low protein diet group was poor, however, the difference was not significant. Carcass backfat thickness in low-protein diet group was slightly higher than that in control group, the difference was significant ( P 〈 0.05 ). There was no significant difference in input-output ratio between low-protein diet group and control group. Finalweight of growing-finishing pigs in low-protein diet group was 3.22 % lower than that in control group, the average daily weight gainwas 6.91% lower than that in control group, feed intake in test group was 5.48% lower than that in control group, and difference between the two treatments was significant (P 〈 0.05 ). Feed meat ratio in low-protein diet group improved, however, the difference was not significant(P 〉0.05). [ Conclusions] Production performance of the pigs would be decreasedand the input-output ratio could not be improvedwhen the adding contents of crude protein in diets was reduced 3% -4%. Therefore, it is recommended that the crude protein level should be enough when pigs were fed with low-protein diets.展开更多
There is growing interest among nutritionists in feeding reduced protein diets to broiler chickens.Although nearly a century of research has been conducted providing biochemical insights on the impact of reduced prote...There is growing interest among nutritionists in feeding reduced protein diets to broiler chickens.Although nearly a century of research has been conducted providing biochemical insights on the impact of reduced protein diets for broilers,practical limitation still exists.The present review was written to provide insights on further reducing dietary protein in broilers.To construct this review,eighty-nine peer reviewed manuscripts in the area of amino acid nutrition in poultry were critiqued.Hence,nutritional research areas of low protein diets,threonine,glycine,valine,isoleucine,leucine,phenylalanine,histidine,and glutamine have been assessed and combined in this text,thus providing concepts into reduced protein diets for broilers.In addition,linkages between the cited work and least cost formation ingredient and nutrient matrix considerations are provided.In conclusion,practical applications in feeding reduced protein diets to broilers are advancing,but more work is warranted.展开更多
Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino...Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.展开更多
Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet w...Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet with supplemental amino acid produce less heat compared to those fed a high crude protein(HCP)diet under both thermal neutral(TN)and heat stress(HS)conditions.Methods:Thirty-two lactating sows were allotted to HCP(193 g CP/kg)and LCP(140 g CP/kg)diets under thermal neutral(TN,21±1.5°C)or cycling heat stress(HS,32±1.5°C daytime and 24±1.5°C nighttime)conditions.Diets contained 0.90%SID lysine and 10.8 MJ/kg net energy.Positive pressure indirect calorimeters were used to measure gas exchange in individual sows with litters,and individual piglets on days 4,8,14 and 18.Sow and litter weights were recorded on days 1,10 and 21.Results:Sow total heat production(THP)was calculated by subtracting litter THP from sow+litter THP based on BW0.75.Sow BW and body protein(BP)loss was greater for LCP diet compared to HCP diet in peak lactation(P<0.05 and P<0.01,respectively)and throughout the entire lactation period(P<0.05 and P=0.056,respectively)under HS conditions.Heat-stressed sows fed HCP diet had higher(P<0.05)rectal temperature at 13:00(P<0.05)and 19:00(P<0.01),and higher respiration rate at 07:00(P<0.05),13:00(P<0.05)and 19:00(P<0.05)compared to TN sows fed HCP diet.In sows fed LCP diet,those under HS tended to have higher(P=0.098)rectal temperature at 13:00 and had higher(P<0.05)respiration rate at 07:00,13:00 and 19:00 compared to TN sows.The relationship between daily THP and days in lactation of sows fed LCP diet was quadratic(P<0.05),with an ascending trend until day 14 and a descending trend from days 14 to 18.Sows fed LCP diet had lower daily THP at day 18(P<0.001)compared to those fed the HCP diet under HS conditions.Conclusion:Reduction in THP in sows fed LCP diet was largely associated with THP on day 18 of lactation under HS conditions.Feeding LCP diets alleviated the increased body temperature in sows under HS conditions throughout lactation,which was accompanied by a reduction in respiration rate.Total heat production is associated with days in lactation,in particular under HS conditions with THP appearing to peak between days 14 and 18.展开更多
To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice...To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice genotypes, transaminase activities and AA levels in grains during GF stage and the traits that significantly affected rice quality (physical appearance, cooking quality, and nutritional value) were analyzed. LL did not disturb transamination in rice grains during GF stage, as minimal impact was found on alanine and aspartate transaminase activities. Nevertheless, most AAs in caryopses, including lysine and threonine, increased in response to LL, except for sulfur-containing AAs. These results suggest that AA metabolism and accumulation in rice grains were rarely suppressed by LL during GF stage. Rice nutritional ingredients at harvest, such as major protein components including glutelin and most important essential amino acids (EAAs) including lysine and threonine, increased significantly in response to LL, whereas most protein and EAA ratios were rarely affected. However, LL markedly affected physical appearance of rice grains by reducing brown rice rate, milled rice rate, and 1000-grain weight and increasing the chalkiness rate. In addition, cooking qualities decreased in response to LL, while breakdown values and amylose levels decreased and setback values increased. We concluded that LL during GF stage decreased the cooking quality of rice, but could potentially improve the nutritional value of rice.展开更多
The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Fo...The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Following 2 weeks of adaptation,48 young barrows were weight matched and randomly assigned to 6 treatments(8 pigs/treatment)for 4 weeks:positive control(PC)with standard protein,negative control(NC)with very low protein containing LAA(i.e.,Lys,Met,Thr and Trp)at rec-ommended levels,and NC containing LAA 25%(L25),LAA 50%(L50),LAA+BCAA(i.e.,Leu,Ile and Val)25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Feed intake(FI)and body weight(BW)were measured daily and weekly,respectively.At week 6,blood samples were collected,all pigs euthanized and tissue samples collected.The data were analyzed by univariate GLM or mixed procedure(SPSS)and the means were separated using paired Student's t-test followed by Benjamini-Hochberg correction.Relative to PC,NC had decreased FI,BW,unsupplemented plasma essential amino acids,serum insulin-like growth factor-I(IGF-I)and hypothalamic neuropeptide Y(NPY)(P<0.01).Compared to NC,L25 or L50,LB50 had increased BW and serum IGF-I and decreased plasma serotonin and both LB25 and LB50 had higher FI,plasma BCAA,hypothalamic 5-hydroxytryptamine-receptor 2A and NPY and jejunal 5-hydroxytryptamine-receptor 7(P<0.01).Overall,supplementation of protein-restricted diets with increased levels of dietary BCAA partially recovered the negative effects of these diets on growth through improved IGF-I concentration and FI,which was associated with changed expression of serotonin receptors,blood AA and hypothalamic NPY.展开更多
基金funded by USDA-NIFA Hatch Fund(#02893,Washington DC,USA)North Carolina Agricultural Foundation(#660101,Raleigh,NC,USA)+3 种基金Ajinomoto Co.,Inc(Tokyo,Japan)CJ Cheil Jedang Corp.(Seoul,Korea)Daesang Corp(Seoul,Korea)Fellowship to support MLTA from CNPq(Brasilia,Brazil).CNPq 305869/2018-3 to support MLTA。
文摘Background Low crude protein(CP)formulations with supplemental amino acids(AA)are used to enhance intestinal health,reduce costs,minimize environmental impact,and maintain growth performance of pigs.However,extensive reduction of dietary CP can compromise growth performance due to limited synthesis of non-essential AA and limited availability of bioactive compounds from protein supplements even when AA requirements are met.Moreover,implementing a low CP formulation can increase the net energy(NE)content in feeds causing excessive fat deposition.Additional supplementation of functional AA,coupled with low CP formulation could further enhance intestinal health and glucose metabolism,improving nitrogen utilization,and growth performance.Three experiments were conducted to evaluate the effects of low CP formulations with supplemental AA on the intestinal health and growth performance of growing-finishing pigs.Methods In Exp.1,90 pigs(19.7±1.1 kg,45 barrows and 45 gilts)were assigned to 3 treatments:CON(18.0%CP,supplementing Lys,Met,and Thr),LCP(16.0%CP,supplementing Lys,Met,Thr,Trp,and Val),and LCPT(16.1%CP,LCP+0.05%SID Trp).In Exp.2,72 pigs(34.2±4.2 kg BW)were assigned to 3 treatments:CON(17.7%CP,meeting the requirements of Lys,Met,Thr,and Trp);LCP(15.0%CP,meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and VLCP(12.8%CP,meeting Lys,Thr,Trp,Met,Val,Ile,Phe,His,and Leu).In Exp.3,72 pigs(54.1±5.9 kg BW)were assigned to 3 treatments and fed experimental diets for 3 phases(grower 2,finishing 1,and finishing 2).Treatments were CON(18.0%,13.8%,12.7%CP for 3 phases;meeting Lys,Met,Thr,and Trp);LCP(13.5%,11.4%,10.4%CP for 3 phases;meeting Lys,Thr,Trp,Met,Val,Ile,and Phe);and LCPG(14.1%,12.8%,11.1%CP for 3 phases;LCP+Glu to match SID Glu with CON).All diets had 2.6 Mcal/kg NE.Results In Exp.1,overall,the growth performance did not differ among treatments.The LCPT increased(P<0.05)Claudin-1 expression in the duodenum and jejunum.The LCP and LCPT increased(P<0.05)CAT-1,4F2hc,and B0AT expressions in the jejunum.In Exp.2,overall,the VLCP reduced(P<0.05)G:F and BUN.The LCP and VLCP increased(P<0.05)the backfat thickness(BFT).In Exp.3,overall,growth performance and BFT did not differ among treatments.The LCPG reduced(P<0.05)BUN,whereas increased the insulin in plasma.The LCP and LCPG reduced(P<0.05)the abundance of Streptococcaceae,whereas the LCP reduced(P<0.05)Erysipelotrichaceae,and the alpha diversity.Conclusions When implementing low CP formulation,CP can be reduced by supplementation of Lys,Thr,Met,Trp,Val,and Ile without affecting the growth performance of growing-finishing pigs when NE is adjusted to avoid increased fat deposition.Supplementation of Trp above the requirement or supplementation of Glu in low CP formulation seems to benefit intestinal health as well as improved nitrogen utilization and glucose metabolism.
基金Supported by"Pig-based Eco-friendly Feed Research and Demonstration"of Science and Technology Department in Hebei Province(11220422)Technology Research on Application of"Animal-derived Active Red Gold Protein Peptide"in Pig Breeding(16226601D)
文摘[ Objectives ] To study the effects of low-protein diets supplemented with amino acids on the production performance, carcass quality and input-output ratio of fattening pigs and growing-finishing pigs. [ Methods] Two studies were conducted on fattening pigs and growing pigs respectively. Pigs in two experimental groupswere fed with low-protein diets supplemented with amino acids and normal diets respectively, and the initial weight, final weight, feed consumption and car- cass index of the tested pigs were measured. [ Results] The average final weight of fattening pigs in test group was 2.4 kg lower than that in control group, while the difference was not significant (P 〉 0.05 ). The average daily gain of fattening pigs in test group was 60 g lower than that in control group, indicating that low protein diets had a tendency to decrease the growth rate (P 〉 0.05 ), and reduction of daily weight gain in low protein diet pigs reached 7.6%. The development of pigs in low protein diet group was poor, however, the difference was not significant. Carcass backfat thickness in low-protein diet group was slightly higher than that in control group, the difference was significant ( P 〈 0.05 ). There was no significant difference in input-output ratio between low-protein diet group and control group. Finalweight of growing-finishing pigs in low-protein diet group was 3.22 % lower than that in control group, the average daily weight gainwas 6.91% lower than that in control group, feed intake in test group was 5.48% lower than that in control group, and difference between the two treatments was significant (P 〈 0.05 ). Feed meat ratio in low-protein diet group improved, however, the difference was not significant(P 〉0.05). [ Conclusions] Production performance of the pigs would be decreasedand the input-output ratio could not be improvedwhen the adding contents of crude protein in diets was reduced 3% -4%. Therefore, it is recommended that the crude protein level should be enough when pigs were fed with low-protein diets.
文摘There is growing interest among nutritionists in feeding reduced protein diets to broiler chickens.Although nearly a century of research has been conducted providing biochemical insights on the impact of reduced protein diets for broilers,practical limitation still exists.The present review was written to provide insights on further reducing dietary protein in broilers.To construct this review,eighty-nine peer reviewed manuscripts in the area of amino acid nutrition in poultry were critiqued.Hence,nutritional research areas of low protein diets,threonine,glycine,valine,isoleucine,leucine,phenylalanine,histidine,and glutamine have been assessed and combined in this text,thus providing concepts into reduced protein diets for broilers.In addition,linkages between the cited work and least cost formation ingredient and nutrient matrix considerations are provided.In conclusion,practical applications in feeding reduced protein diets to broilers are advancing,but more work is warranted.
基金funded by Animal Health and Production and Animal Products:Improved Nutritional Performance,Growth,and Lactation of Animals,grant no. 2021–67016-33399/project accession no. 1024334Hatch project accession no. 1012889 from the USDA National Institute of Food and Agriculture (NIFA)
文摘Background:Very low-protein(VLP)diets negatively impact calcium(Ca)metabolism and absorption.The objective of this study was to investigate the effect of supplemental branched-chain amino acids(BCAA)and limiting amino acids(LAA)on Ca digestibility,absorption and reabsorption in pigs fed with VLP diets.Forty-eight piglets were assigned to six treatments:positive control(PC),negative control(NC),and NC containing LAA 25%,LAA 50%,LAA+BCAA 25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Results:Relative to PC or NC,LB25 and LB50 had higher digestibility of Ca and plasma Ca and phosphorus(P),but lower plasma vitamin D3.LB50 tended to increase vitamin D receptor transcript and protein in the gut,but decreased mRNA or protein abundance of parathyroid hormone 1 receptor(PTH1R),calbindin 1(CALB1),cytochrome P450 family 27 subfamily B member 1 and occludin in small intestine.LB50 increased the transcript of cytochrome P450 family 24 subfamily A member 1 and PTH1R but decreased the transcript of transient receptor potential cation channel subfamily V member 5,CALB1 and solute carrier family 17 member 4 in kidney.Conclusion:Overall,BCAA increased Ca digestibility through regulating the transcellular and paracellular Ca absorption in the gut and reabsorption in kidney during protein restriction.
基金supported by the USDA-NIFA(award number 2014–67015-21832).
文摘Background:Reduced protein diet manifested potential to mitigate heat production based on the concept of ideal amino acid profile.The hypothesis of this study was that lactating sows fed a low crude protein(LCP)diet with supplemental amino acid produce less heat compared to those fed a high crude protein(HCP)diet under both thermal neutral(TN)and heat stress(HS)conditions.Methods:Thirty-two lactating sows were allotted to HCP(193 g CP/kg)and LCP(140 g CP/kg)diets under thermal neutral(TN,21±1.5°C)or cycling heat stress(HS,32±1.5°C daytime and 24±1.5°C nighttime)conditions.Diets contained 0.90%SID lysine and 10.8 MJ/kg net energy.Positive pressure indirect calorimeters were used to measure gas exchange in individual sows with litters,and individual piglets on days 4,8,14 and 18.Sow and litter weights were recorded on days 1,10 and 21.Results:Sow total heat production(THP)was calculated by subtracting litter THP from sow+litter THP based on BW0.75.Sow BW and body protein(BP)loss was greater for LCP diet compared to HCP diet in peak lactation(P<0.05 and P<0.01,respectively)and throughout the entire lactation period(P<0.05 and P=0.056,respectively)under HS conditions.Heat-stressed sows fed HCP diet had higher(P<0.05)rectal temperature at 13:00(P<0.05)and 19:00(P<0.01),and higher respiration rate at 07:00(P<0.05),13:00(P<0.05)and 19:00(P<0.05)compared to TN sows fed HCP diet.In sows fed LCP diet,those under HS tended to have higher(P=0.098)rectal temperature at 13:00 and had higher(P<0.05)respiration rate at 07:00,13:00 and 19:00 compared to TN sows.The relationship between daily THP and days in lactation of sows fed LCP diet was quadratic(P<0.05),with an ascending trend until day 14 and a descending trend from days 14 to 18.Sows fed LCP diet had lower daily THP at day 18(P<0.001)compared to those fed the HCP diet under HS conditions.Conclusion:Reduction in THP in sows fed LCP diet was largely associated with THP on day 18 of lactation under HS conditions.Feeding LCP diets alleviated the increased body temperature in sows under HS conditions throughout lactation,which was accompanied by a reduction in respiration rate.Total heat production is associated with days in lactation,in particular under HS conditions with THP appearing to peak between days 14 and 18.
文摘To investigate the effect of low light (LL, 50% natural light) during grain filling (GF) stage on rice transamination, amino acid (AA) accumulation, nutritional value, and cooking quality in three different rice genotypes, transaminase activities and AA levels in grains during GF stage and the traits that significantly affected rice quality (physical appearance, cooking quality, and nutritional value) were analyzed. LL did not disturb transamination in rice grains during GF stage, as minimal impact was found on alanine and aspartate transaminase activities. Nevertheless, most AAs in caryopses, including lysine and threonine, increased in response to LL, except for sulfur-containing AAs. These results suggest that AA metabolism and accumulation in rice grains were rarely suppressed by LL during GF stage. Rice nutritional ingredients at harvest, such as major protein components including glutelin and most important essential amino acids (EAAs) including lysine and threonine, increased significantly in response to LL, whereas most protein and EAA ratios were rarely affected. However, LL markedly affected physical appearance of rice grains by reducing brown rice rate, milled rice rate, and 1000-grain weight and increasing the chalkiness rate. In addition, cooking qualities decreased in response to LL, while breakdown values and amylose levels decreased and setback values increased. We concluded that LL during GF stage decreased the cooking quality of rice, but could potentially improve the nutritional value of rice.
基金funded by Animal Health and Production and Animal Products:Improved Nutritional Performance,Growth,and Lactation of Animals grant no.2018-67016-27471/project accession no.1014892,grant no.2021-67016-33399/project accession no.1024334Hatch project accession no.1012889 from the USDA National Institute of Food and Agriculture
文摘The objective of this study was to assess the growth efficiency of pigs fed with protein-restricted diets supplemented with branched-chain amino acids(BCAA)and limiting amino acids(LAA)above the rec-ommended levels.Following 2 weeks of adaptation,48 young barrows were weight matched and randomly assigned to 6 treatments(8 pigs/treatment)for 4 weeks:positive control(PC)with standard protein,negative control(NC)with very low protein containing LAA(i.e.,Lys,Met,Thr and Trp)at rec-ommended levels,and NC containing LAA 25%(L25),LAA 50%(L50),LAA+BCAA(i.e.,Leu,Ile and Val)25%(LB25)and LAA+BCAA 50%(LB50)more than recommendations.Feed intake(FI)and body weight(BW)were measured daily and weekly,respectively.At week 6,blood samples were collected,all pigs euthanized and tissue samples collected.The data were analyzed by univariate GLM or mixed procedure(SPSS)and the means were separated using paired Student's t-test followed by Benjamini-Hochberg correction.Relative to PC,NC had decreased FI,BW,unsupplemented plasma essential amino acids,serum insulin-like growth factor-I(IGF-I)and hypothalamic neuropeptide Y(NPY)(P<0.01).Compared to NC,L25 or L50,LB50 had increased BW and serum IGF-I and decreased plasma serotonin and both LB25 and LB50 had higher FI,plasma BCAA,hypothalamic 5-hydroxytryptamine-receptor 2A and NPY and jejunal 5-hydroxytryptamine-receptor 7(P<0.01).Overall,supplementation of protein-restricted diets with increased levels of dietary BCAA partially recovered the negative effects of these diets on growth through improved IGF-I concentration and FI,which was associated with changed expression of serotonin receptors,blood AA and hypothalamic NPY.