Enhancement of porcine in vitro embryonic development through luteolin‑mediated activation of the Nrf2/Keap1 signaling pathway

Background Oxidative stress,caused by an imbalance in the production and elimination of intracellular reactive oxygen species(ROS),has been recognized for its detrimental effects on mammalian embryonic development.Luteolin(Lut)has been documented for its protective effects against oxidative stress in various studies.However,its specific role in embryonic development remains unexplored.This study aims to investigate the influence of Lut on porcine embryonic development and to elucidate the underlying mechanism.Results After undergoing parthenogenetic activation(PA)or in vitro fertilization,embryos supplemented with 0.5μmol/L Lut displayed a significant enhancement in cleavage and blastocyst formation rates,with an increase in total cell numbers and a decrease in the apoptosis rate compared to the control.Measurements on D2 and D6 revealed that embryos with Lut supplementation had lower ROS levels and higher glutathione levels compared to the control.Moreover,Lut supplementation significantly augmented mitochondrial content and membrane potential.Intriguingly,activation of the Nrf2/Keap1 signaling pathway was observed in embryos supplemented with Lut,leading to the upregulation of antioxidant-related gene transcription levels.To further validate the relationship between the Nrf2/Keap1 signaling pathway and effects of Lut in porcine embryonic development,we cultured PA embryos in a medium supplemented with brusatol,with or without the inclusion of Lut.The positive effects of Lut on developmental competence were negated by brusatol treatment.Conclusions Our findings indicate that Lut-mediated activation of the Nrf2/Keap1 signaling pathway contributes to the enhanced production of porcine embryos with high developmental competence,and offers insight into the mechanisms regulating early embryonic development.

Pathway and Countermeasure of Farmers' Livelihood Transformation in the Ecological Development Area of Northern Guangdong Province from the Perspective of Ecological Protection

[Objectives]To explore pathways and countermeasures for transforming farmers livelihoods in the way of reducing their dependence on land while promoting sustainable development and alleviating ecological degradation.[Methods]A combination of field research,literature review,and policy analysis was employed to identify key factors affecting farmers livelihoods and potential strategies for transformation.[Results]The study found that developing ecological agriculture and modern agriculture,promoting agricultural transformation and upgrading,cultivating alternative industries,strengthening ecological engineering construction,and establishing diversified ecological compensation methods and supporting policies are effective strategies for transforming farmers livelihoods.[Conclusions]Implementing these strategies can help alleviate the contradiction between ecological protection and farmers livelihood development,promoting coordinated development of both.This approach not only benefits farmers but also contributes to sustainable environmental management and biodiversity conservation.

A Mechanism for Digital Finance to Drive Green and Low-Carbon Development

Given the global focus on green and low-carbon development and the increasing prominence of digital finance,it is particularly important to explore how to leverage digital finance to achieve these environmental goals.This study,through mechanism analysis,deeply examines how China’s digital finance promotes green and low-carbon development and elucidates the positive interaction between digital finance and the green industry.The study found that digital finance,through more flexible and efficient financial functions,alters the cost structure of carbon emissions,and reduces the risks and costs of green investments,thereby creating a cooperative green mechanism benefiting all parties,and guiding social groups toward a green and low-carbon transformation.Additionally,the rapid development of digital finance has strengthened the implementation of environmental protection policies,effectively promoted the expansion of the environmental protection industry,and established the green ethos as a mainstream concept in financial development.This study aims to provide reference perspectives and suggestions,assist policymakers in promoting the green and lowcarbon development of digital finance,and offer insights into the integrated development of digital finance and the green environmental protection industry.

The synergistic regulatory effect of PTP1B and PTK inhibitors on the development of Oedaleus decorus asiaticus Bei-Bienko

Tyrosine phosphorylation is crucial for controlling normal cell growth,survival,intercellular communication,gene transcription,immune responses,and other processes.protein tyrosine phosphatase(PTP)and protein tyrosine kinases(PTK)can achieve this goal by regulating multiple signaling pathways.Oedaleus decorus asiaticus is an important pest that infests the Mongolian Plateau grassland.We aimed to evaluate the survival rate,growth rate,overall performance,and ovarian developmental morphology of the 4th instar nymphs of O.decorus asiaticus while inhibiting the activity of protein tyrosine phosphatase-1B(PTP1B)and PTK.In addition,the expression and protein phosphorylation levels of key genes in the MAPK signaling pathway and antioxidant enzyme activity were assessed.The results showed no significant differences in survival rate,growth rate,or overall performance between PTP1B inhibitor treatment and control.However,after PTK inhibitor treatment,these indexes were significantly lower than those in the control.The ovarian size of female larvae after 15 days of treatment with PTK inhibitors showed significantly slower development,while female larvae treated with PTP1B exhibited faster ovarian growth than the control group.In comparison to controls and nymphs treated with PTK inhibitors,the expression and phosphorylation levels of key genes in the MAPK signaling pathway under PTP1B inhibitor treatments were significantly higher in 4th instar nymphs.However,reactiveoxygen(ROS)species levels and the activities of NADPH oxidase and other antioxidant enzymes were considerably reduced,although they were significantly greater in the PTK inhibitor treatment.The results suggest that PTP1B and PTK feedback inhibition in the mitogen-activated-protein kinases(MAPK)signal transfer can regulate the physiological metabolism of the insect as well as its developmental rate.These findings can facilitate future uses of PTP1B and PTK inhibitors in controlling insect development to help control pest populations.

Anethole improves the developmental competence of porcine embryos by reducing oxidative stress via the sonic hedgehog signaling pathway

Background Anethole(AN)is an organic antioxidant compound with a benzene ring and is expected to have a positive impact on early embryogenesis in mammals.However,no study has examined the effect of AN on porcine embryonic development.Therefore,we investigated the effect of AN on the development of porcine embryos and the underlying mechanism.Results We cultured porcine in vitro-fertilized embryos in medium with AN(0,0.3,0.5,and 1 mg/mL)for 6 d.AN at 0.5 mg/mL significantly increased the blastocyst formation rate,trophectoderm cell number,and cellular survival rate compared to the control.AN-supplemented embryos exhibited significantly lower reactive oxygen species levels and higher glutathione levels than the control.Moreover,AN significantly improved the quantity of mitochondria and mitochondrial membrane potential,and increased the lipid droplet,fatty acid,and ATP levels.Interestingly,the levels of proteins and genes related to the sonic hedgehog(SHH)signaling pathway were significantly increased by AN.Conclusions These results revealed that AN improved the developmental competence of porcine preimplantation embryos by activating SHH signaling against oxidative stress and could be used for large-scale production of high-quality porcine embryos.

Regulation of bone phosphorus retention and bone development possibly by BMP and MAPK signaling pathways in broilers

The bone morphogenetic protein(BMP)and mitogen-activated protein kinase(MAPK)signaling pathways play an important role in regulation of bone formation and development,however,it remains unclear that the effect of dietary different levels of non-phytate phosphorus(NPP)on these signaling pathways and their correlations with bone phosphorus(P)retention and bone development in broilers.Therefore,this experiment was conducted to investigate the effect of dietary P supplementation on BMP and MAPK signaling pathways and their correlations with bone P retention and bone development in broilers.A total of 800 one-day-old Arbor Acres male broilers were randomly allotted to 1 of 5 treatments with 8 replicates in a completely randomized design.The 5 treatments of dietary NPP levels were 0.15,0.25,0.35,0.45 and 0.55%or 0.15,0.22,0.29,0.36 and 0.43%for broilers from 1 to 21 days of age or 22 to 42 days of age,respectively.The results showed that extracellular signal-regulated kinase 1(ERK1)mRNA expression in the tibia of broilers on days 14 and 28,phosphorylated-ERK1(p-ERK1)on day 14,and BMP2 protein expression on days 28 and42 decreased linearly(P<0.04),while c-Jun N-terminal kinase 1(JNK1)mRNA expression on day 42 increased linearly(P<0.02)with the increase of dietary NPP level.At 14 days of age,total P accumulation in tibia ash(TP),bone mineral concentration(BMC),bone mineral density(BMD),bone breaking strength(BBS)and tibia ash were negatively correlated(r=-0.726 to-0.359,P<0.05)with ERK1 and JNK1 mRNA as well as p-ERK1;tibia alkaline phosphatase(ALP)and bone gal protein(BGP)were positively correlated(r=0.405 to 0.665,P<0.01)with ERK1 mRNA and p-ERK1.At 28 days of age,TP,BMC,BMD,BBS and tibia ash were negatively correlated(r=-0.518 to-0.370,P<0.05)with ERK1 mRNA and BMP2 protein,while tibia ALP was positively correlated(r=0.382 to 0.648,P<0.05)with them.The results indicated that TP,BMC,BMD,BBS or tibia ash had negative correlations,while tibia ALP and BGP had positive correlations with ERK1 and JNK1 mRNAs,BMP2 protein and p-ERK1,suggesting that bone P retention and bone development might be regulated by BMP and MAPK signaling pathways in broiler chickens.

Low-carbon Agriculture Development Models in Southern Jiangsu Province

[Objective] To investigate the appropriate low-carbon agriculture model in Southern Jiangsu Province. [Method] Through the analysis of regional features in Southern Jiangsu and several matured low-carbon agriculture development models at present, the low-carbon agriculture development modes suitable for Southern Jiangsu were investigated, and corresponding supporting measures for the development of the models were put forward. [Result] Low-carbon agriculture is the environment- friendly agriculture which achieves low emissions, low pollution, high efficiency and high-yield through efficient recycling of energy and resources and continuous im- provements on ecological environment. With a variety of development models, the specific development model for practical use should be determined according to the local conditions, and supported by corresponding supporting measures, to achieve the rapid development of low-carbon agriculture. [Conclusion] This study laid the foundation for the development of low-carbon agriculture model in Southern Jiangsu.

Development path of Chinese low-carbon cities based on index evaluation

This work takes the 36 cities from China＇s low-carbon pilot project as the research object and uses the carbon emission per capita and GDP per capita to categorize the 36 cities into four types to reveal their low-carbon development status; these four types are leading cities, developing cities, latecomer cities, and exploring cities. On the basis of an index system that quantitatively describes low-carbon development, this research analyzes the characteristics, development trends, and low-carbon development pathways of the four types of cities. According to the present situation and objectives of national emissions and considering the differences in development stages, challenges, and opportunities for each type of the city, this research presents recommendations for the low-carbon roadmap and the medium- and long-term （by 2030） emission trend routes of different types of regions in China.

Effects of dietary tributyrin on intestinal mucosa development,mitochondrial function and AMPK-mTOR pathway in weaned pigs

Background:The objective of this experiment was to investigate the influence of dietary tributyrin on intestinal mucosa development,oxidative stress,mitochondrial function and AMPK-mTOR signaling pathway.Methods:Seventy-two pigs were divided into two treatments and received either a basal diet or the same diet supplemented with 750 mg/kg tributyrin.Each treatment has six replicates of six pigs.After 14 days,6 pigs from each treatment were selected and the jejunal samples were collected.Results:Results showed that supplemental tributyrin increased(P<0.05)villus height and villus height:crypt depth of weaned pigs.Pigs fed tributyrin had greater(P<0.05)RNA/DNA and protein/DNA ratios than pigs on the control group.The mRNA levels of sodium glucose transport protein-1 and glucose transporter-2 in the jejunum were upregulated(P<0.05)in pigs fed the tributyrin diet.Dietary tributyrin supplementation lowered(P<0.05)the malondialdehyde and hydrogen peroxide(H2O2)content in jejunum,enhanced(P<0.05)the mitochondrial function,as demonstrated by decreased(P<0.05)reactive oxygen species level and increased(P<0.05)mitochondrial membrane potential.Furthermore,tributyrin increased(P<0.05)mitochondrial DNA content and the mRNA abundance of genes related to mitochondrial functions,including peroxisomal proliferator-activated receptor-γcoactivator-1α,mitochondrial transcription factor A,nuclear respiratory factor-1 in the jejunum.Supplementation with tributyrin elevated(P<0.05)the phosphorylation level of AMPK and inhibited(P<0.05)the phosphorylation level of mTOR in jejunum compared with the control group.Conclusions:These findings suggest that dietary supplementation with tributyrin promotes intestinal mucosa growth,extenuates oxidative stress,improves mitochondrial function and modulates the AMPK-mTOR signal pathway of weaned pigs.

Industry Linkage and Low-carbon Development--Based on Logistics Industry & Manufacturing Industry

Key project of " manufacturing industry and logistics industry linkage"was proposed in the Logistics Adjustment and Revitalization Plan by the state council in 2009. However the consumption and pollution generated by manufacturing industry and logistics industry linkage in China are also large at present. How to conduct manufacturing industry and logistics industry linkage by the low-carbon manner is one of most important issues under current low-carbon economy background. In this paper,the issue is studied and analyzed by constructing system dynamics model,which could propose suggestions for low-carbon linkage development of manufacturing industry and logistics industry.

High speed railway promoting development of low-carbon economy in China

Low-carbon Economy is a kind of economic developing mode which takes low energy consumption,low pollution and low discharge as its foundation. It is another important revolution of the human society after the agriculture civilization and industry civilization. It deals with various fields including low-carbon energy,low-carbon agriculture,low-carbon industry,low-carbon transportation and low-carbon life,etc. Among those,low-carbon transportation is one of the important contents. Since high speed railway uses electricity as driving force,it runs without discharging waste gas,and it is a kind of clean and green transportation with little dust and smoke black. Therefore,the study of the relationship between the high speed railway and low-carbon economy is becoming one of the important frontier problems that confronting the theoretic circle. With demonstration analysis and comparative analysis,this paper discusses the comparative advantages of high speed railway in terms of energy saving and environment protection,treatment of sound pollution,land saving and reduction of external cost(mainly pollution treatment cost) ,etc. compared with the other transportation modes and ordinary railway. Taking Beijing-Tianjin Intercity Railway as an example,the paper further demonstrates the distinctive advantages in respect of energy conservation and emission reduction. Besides,the paper also predicates the low-carbon effects after several high speed railways is put into operation in a few years. It is concluded that the development of high speed railways will meet the need of low-carbon economy and is significant for sustainable and steady development of economy and society.

Development Paths of Low-carbon Landscape Design

Through interpreting significance of low-carbon landscape, and analyzing common problems in landscape design industry, paths of low-carbon landscape design were explored from the following perspectives: establishing low-carbon index system for the landscape construction of eco-city; constructing scientific and reasonable spatial layout; using landscapes to interpret low-carbon concepts; enhancing the carbon sink function of urban green spaces; choosing reasonable landscape materials; reducing landscape maintenance cost. The study aims at applying low-carbon concepts in all design projects to achieve sustainable development of urban landscapes.

Development Trend of Energy and Key Points of Ecological Environment Protection in China's "14^(th) Five-Year Plan" Period

From the changes of total primary energy consumption and primary energy consumption structure,installed capacity and power generation of secondary energy(electricity),etc.,the development characteristics of China's energy structure in the"13^(th) Five-Year Plan"period was comprehensively studied.Combined with the complexity of the international situation,the diversification of the domestic energy system,the intelligence of the energy industry,etc.,the situation and challenges faced by China's energy development were deeply discussed in the four aspects of energy security,energy low-carbon transformation,energy system efficiency,and energy technology level.From the angle of ecological environment protection,the development of energy in the"14^(th) Five-Year Plan"period in China was analyzed,and some policies and measures were put forward.

The Current Status and Prospects of China’s Low- Carbon Economic Development

This paper aims to explore the importance of developing a low-carbon economy in China and presents a series of key recommendations.Firstly,it emphasizes the adverse impact of economic development on the natural environment and highlights the unsustainable nature of China’s previous pursuit of economic growth at the expense of its ecological surroundings.Subsequently,the paper provides specific recommendations in response to the pressing resource and environmental challenges in current economic and social development,along with the urgent need to accelerate the transition towards a greener,low-carbon economy to achieve carbon peak and carbon neutrality goals.The key recommendations include continually enhancing the legal framework for a low-carbon economy,optimizing the energy structure,improving energy efficiency,and optimizing the industrial structure.These proposals aim to offer concrete actions to support the development of a low-carbon economy in China,encompassing legal support,energy transformation,and industrial upgrading.Ultimately,this paper underscores China’s potential in developing a low-carbon economy and calls for resolute actions to strengthen legal frameworks,foster technological innovation,and promote industrial restructuring.These actions will guide China towards a more sustainable and environmentally conscious economic future,aligning its growth trajectory with the global imperative to reduce carbon footprints.

mTOR Signaling Pathway and Its Regulation on Growth and Development

The mammalian target of rapamycin （mTOR） signaling pathway is evolutionarily conserved, mTOR can integrate and converge a wide range of signals, including intracellular and extracellular nutrients, growth factors, energy and stress conditions, and has a crucial role in the vertebrate growth control. This review analyzed the main components and regulated factors of TOR signaling pathway, explained functions and mechanisms of roTOR during the individual growth, the development and its dynamic role, revealed its additional functions beyond the cell growth control, and finally reviewed the tissue specificity and time specificity of mTOR signaling pathway, and its regulation on sexual differentiation, tissue differentiation and organogenesis in the individual development.

Development and distribution of PAG-immunoreactive neurons in the central pathway of trigeminal proprioception of the rat brainstem*

Objective: To investigate the development and distribution of phosphate-activated glutaminase like immunoreactive (PAG-LI) neurons in the central pathway of trigeminal proprioception of the rat brainstem. Methods: The immunohistochemitry techniques were used. Results: (1) At embryonic day 17 (E17), PAG-LI neurons were initially observed in the mesencephalic trigeminal nucleus (Vme). All PAG-LI neurons were large round neurons with moderate immunostaining. The immunoreactivity grew intense and attained adult-like pattern at P10. (2) Not until postnatal day 10 (P10) did a few PAG-LI neurons appear in the area ven-tral to the motor trigeminal nucleus (AVM) and area dorsal to the superior olivery nucleus (ADO), and not until P12 in the dorsomedial part of the subnucleus oralis of the spinal trigeminal nucleus (Vodm) and dorso-medial part of the principal sensory trigeminal nucleus (Vpdm). As development proceeded, more and more neurons in them were immunostained, and some PAG-LI neurons were detected in the lateral reticular forma-tion adjacent to the Vodm(LRF)and the caudolateral part of the supratrigeminal nucleus (Vsup-CL) at P21. Conclusion: In the central pathway of trigeminal proprioception of the rat brainstem, PAG-LI neurons ap-peared during two stages: The first stage from E17 to P10, PAG-LI neurons appeared in the Vme and reached adult-like pattern; the second stage from P10 to P21, PAG-LI neurons appeared in the Vodm, LRF, Vpdm, Vsup-CL, ADO, AVM and gradually reached adult-like pattern. This might be relative to the estab-lishment of jaw movement patterns.

Expression Profile Analysis of Genes Involved in Brassinosteroid Biosynthesis Pathway in Cotton Fiber Development

Cotton(Gossypium hirsutum L.) is the leading fiber crop and one of the mainstays of the economy in the world.Cotton fibers,as the main product of cotton plants,are unicellular,linear

Research on low-carbon development of electric power industry:from the perspectives of public policy

It is a fundamental way to achieve sustainable development and inclusive growth that China takes the low-carbon development path.And low-carbon development is an effective way to respond to many threats,including global climate anomalies,environmental degradation,and energy shortage.Low-carbon economy is policy economy to a large extent.Although the external environment demands low-carbon development of electric power industry,enterprises lack intrinsic motivation.Electric power industry is the pillar industry of China,and is one of the key industries for China's low-carbon development.The government needs to ensure its dominant position in the low-carbon development of electric power industry,and reform the government performance management system and promote the planning and management of electric power industry through appropriate public policy,in order to adjust the structure of the electric power industry and take a low-carbon development road with Chinese characteristics.

Pathway Planning of Nuclear Power Development Incorporating Assessment of Nuclear Event Risk

The nuclear event risk(NER)is an important and disputed factor that should be reasonably considered when planning the pathway of nuclear power development(NPD)to assess the benefits and risks of developing nuclear power more objectively.This paper aims to explore the impact of nuclear events on NPD pathway planning.The influence of nuclear events is quantified as a monetary risk component,and an optimization model that incorporates the NER in the objective function is proposed.To optimize the pathway of NPD in the lowcarbon transition course of power supply structure evolution,a simulation model is built to deduce alternative NPD pathways and corresponding power supply evolution scenarios under the constraint of an exogenously assigned carbon emission pathway(CEP);moreover,a method is proposed to describe the CEP by superimposing the maximum carbon emission space and each carbon emission reduction(CER)component,and various CER components are clustered considering the emission reduction characteristics and resource endowments of different power generation technologies.A case study is conducted to explore the impact of NER and its risk valuation uncertainty on NPD pathway planning.The method presented in this paper allows the impact of nuclear events on NPD pathway planning to be quantified and improves the level of coordinated optimization of benefits and risks.

The molecular genetics of PI3K/PTEN/AKT/mTOR pathway in the malformations of cortical development

Malformations of cortical development(MCD)are a group of developmental disorders characterized by abnormal cortical structures caused by genetic or harmful environmental factors.Many kinds of MCD are caused by genetic variation.MCD is the common cause of intellectual disability and intractable epilepsy.With rapid advances in imaging and sequencing technologies,the diagnostic rate of MCD has been increasing,and many potential genes causing MCD have been successively identified.However,the high genetic heterogeneity of MCD makes it challenging to understand the molecular pathogenesis of MCD and to identify effective targeted drugs.Thus,in this review,we outline important events of cortical development.Then we illustrate the progress of molecular genetic studies about MCD focusing on the PI3K/PTEN/AKT/mTOR pathway.Finally,we briefly discuss the diagnostic methods,disease models,and therapeutic strategies for MCD.The information will facilitate further research on MCD.Understanding the role of the PI3K/PTEN/AKT/mTOR pathway in MCD could lead to a novel strategy for treating MCD-related diseases.