Low-frequency pulsed electromagnetic field pretreated bone marrow-derived mesenchymal stem cells promote the regeneration of crush-injured rat mental nerve

Bone marrow-derived mesenchymal stem cells （BMSCs） have been shown to promote the regeneration of injured peripheral nerves. Pulsed electromagnetic field （PEMF） reportedly promotes the proliferation and neuronal differentiation of BMSCs. Low-frequency PEMF can induce the neuronal differentiation of BMSCs in the absence of nerve growth factors. This study was designed to investigate the effects of low-frequency PEMF pretreatment on the proliferation and function of BMSCs and the effects of low-frequency PEMF pre-treated BMSCs on the regeneration of injured peripheral nerve using in vitro and in vivo experiments.In in vitro experiments, quantitative DNA analysis was performed to determine the proliferation of BMSCs, and reverse transcription-polymerase chain reaction was performed to detect S100 （Schwann cell marker）, glial fibrillary acidic protein （astrocyte marker）, and brain-derived neurotrophic factor and nerve growth factor （neurotrophic factors） mRNA expression. In the in vivo experiments, rat models of crush-injured mental nerve established using clamp method were randomly injected with low-frequency PEMF pretreated BMSCs, unpretreated BMSCs or PBS at the injury site （1 × 106 cells）. DiI-labeled BMSCs injected at the injury site were counted under the fluorescence microscope to determine cell survival. One or two weeks after cell injection, functional recovery of the injured nerve was assessed using the sensory test with von Frey filaments. Two weeks after cell injection, axonal regeneration was evaluated using histomorphometric analysis and retrograde labeling of trigeminal ganglion neurons. In vitro experiment results revealed that low-frequency PEMF pretreated BMSCs proliferated faster and had greater mRNA expression of growth factors than unpretreated BMSCs. In vivo experiment results revealed that compared with injection of unpretreated BMSCs, injection of low-frequency PEMF pretreated BMSCs led to higher myelinated axon count and axon density and more DiI-labeled neurons in the trigeminal ganglia, contributing to rapider functional recovery of injured mental nerve. These findings suggest that low-frequency PEMF pretreatment is a promising approach to enhance the efficacy of cell therapy for peripheral nerve injury repair.

Effect of low-frequency pulse percutaneous electric stimulation on peripheral nerve injuries at different sites

BACKGROUND： The postoperative recovery of nerve function in patients with peripheral nerve injury is always an important problem to solve after treatment. The electric stimulation induced electromagnetic field can nourish nerve, postpone muscular atrophy, and help the postoperative neuromuscular function. OBJECTIVE： To observe the effects of low-frequency pulse percutaneous electric stimulation on the functional recovery of postoperative patients with peripheral nerve injury, and quantitatively evaluate the results of electromyogram （EMG） examination before and after treatment. DESIGN ： A retrospective case analysis SETTING： The Sixth People＇s Hospital affiliated to Shanghai Jiaotong University PARTICIPANTS： Nineteen postoperative inpatients with peripheral nerve injury were selected from the De- partment of Orthopaedics, the Sixth People＇s Hospital affiliated to Shanghai Jiaotong University from June 2005 to January 2006, including 13 males and 6 females aged 24-62 years with an average of 36 years old. There were 3 cases of brachial plexus nerve injury, 3 of median nerve injury, 7 of radial nerve injury, 3 of ul- nar nerve injury and 3 of common peroneal nerve injury, and all the patients received probing nerve fiber restoration. Their main preoperative manifestations were dennervation, pain in limbs, motor and sensory disturbances. All the 19 patients were informed with the therapeutic program and items for evaluation. METHODS： ① Low-frequency pulse percutaneous electric stimulation apparatus： The patients were given electric stimulation with the TERESA cantata instrument （TERESA-0, Shanghai Teresa Health Technology, Co., Ltd.）. The patients were stimulated with symmetric square waves of 1-111 Hz, and the intensity was 1.2-5.0 mA, and it was gradually adjusted according to the recovered conditions of neural regeneration following the principle that the intensity was strong enough and the patients felt no obvious upset. They were treated for 4- 24 weeks, 10-30 minutes for each time, 1-3 times a day, and 6 weeks as a course. ② EMG examination was applied to evaluate the recoveries of recruitment, motor conduction velocity （MCV） and sensory conduction velocity （SCV） before and after treatment. The patients were examined with the EMG apparatus （DIS- A2000C, Danmark） before and after the treatment of percutaneous electric stimulation. ③Standards for evaluating the effects included cured （complete recovery of motor functions, muscle strength of grade 5, no abnormality in EMG examination）, obviously effective [general recovery of motor function, muscle strength of grade 4, no or a few denervation potentials, motor conduction velocity （MCV） and sensory conduction velocity （SCV）], improved （partial recovery of motor function, muscle strength of grade 3, denervation potentials and reinneration potentials, slowed MCV and SCV, invalid （no obvious changes of motor function）. MAIN OUTCOME MEASURES： ① Ameliorated degree of the nerve function of the postoperative patients with peripheral nerve injury treated with percutaneous electric stimulation; ② Changes of EMG examination before and after treatment. RESULTS： All the 19 postoperative patients with peripheral nerve injury were involved in the analysis of results. ① Comparison of nerve function before and after treatment in 19 patients with peripheral nerve injury of different sites： For the patients with radial nerve injury （n=7）, the nerve functions all completely recovered after 8-week treatment, and the cured and obvious rate was 100% （7/7）; For the patients with brachial plexus nerve injury （n=3）, 1 case had no obvious improvement, and the cured and obvious rate was 67% （2/3）; For the patients with common peroneal nerve injury （n=3）, the extension of foot dorsum generally recovered in 1 case of nerve contusion after 4-week treatment, and the cured and obvious rate was 67% （2/3）; For the patients with median nerve injury （n=3）, muscle strength was obviously recovered, and the cured and obvious rate was 100% （3/3）; For the patients with ulnar nerve injury （n=3）, 1 case only had recovery of partial senses, and the cured and obvious rate was 67% （2/3）. Totally 9 cases were cured, 7 were obviously effective, 1 was improved, and only 2 were invalid. After 4 courses, the cured rate of damaged nerve function after four courses was 47% （9/19）, and effective rate was 89% （17/19）.② Comparison of EMG examination before and after treatment： Before and after percutaneous electric stimulation, he effective rates of recruitment, MCV and SCV were 89% （17/19）, 58% （11/19）, 47% （9/19） respectively, and there were extremely obvious differences （P〈 0.01）. CONCLUSION： ①Low-frequency pulse percutaneous electric stimulation can improve the nerve function of postoperative patients with peripheral nerve injury of different sites, especially that the injuries of radial nerve and median nerve recover more obviously. ②Percutaneous electric stimulation can ameliorate the indexes of EMG examination, especially the recruitment, in postoperative patients with peripheral nerve injury.

Treatment time influences the effects of a low-frequency pulsed electric field on synthesis of tyrosine hydroxylase and dopamine in PC12 cells

BACKGROUND： Electromagnetic radiation can influence dopamine （DA） synthesis in brain tissues or ceils, but electromagnetic frequencies, intensities, and radiation time can produce different effects. In addition, the signal pathway by which electromagnetic radiation influences DA synthesis remains controversial. OBJECTIVE： To determine tyrosine hydroxylase （TH） expression in PC12 cells and DA levels in cell culture media after different periods of low-frequency pulsed electric field （LF-PEF） stimulation, and to determine how LF-PEF signaling stimulates TH synthesis using inhibitors. DESIGN, TIME AND SETTING： A parallel, controlled, cell experiment was performed at the Laboratory of Cell Biology, School of Life Science, East China Normal University, between January and October 2006. MATERIALS： PC12 cells were purchased from the Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, China. Nerve growth factor was purchased from PeproTech, USA. The protein kinase A inhibitor, H-89, and mitogen-activated protein kinase kinase inhibitor, U0126, were purchased from Sigma, USA. METHODS： （1） Following routine culture in Dulbecco＇s modified eagle medium, primary PC12 cells were stimulated under LF-PEF （pulse frequency 50.Hz, pulse width 20 μs, peak field strength 1 V/m） for 5, 10, 15, 20, and 30 minutes. （2） Inhibitors （H-89 or U0126, 1 μmol/L） were added 30 minutes before LF-PEF stimulation for 10 minutes. MAIN OUTCOME MEASURES： （1） TH expression was determined by Western blot in PC12 cells at 0.5, 1,2, 3, and 4 days after LF-PEF stimulation. Similarly, DA was measured by high-performance liquid chromatography in media at 2, 3, 4, or 5 days after LF-PEE （2） TH expression was detected 1 day after H-89 or U0126 treatment and LF-PEE RESULTS： （1） Short-term LF-PEF stimulation （5 and 10 minutes） increased TH expression and media DA levels after short-term culture （2 days） （P 〈 0.01）, but both parameters decreased with longer culture （3 4 days） （P 〈 0.01）. Long-term LF-PEF stimulation （15, 20, or 30 minutes） decreased TH and DA synthesis, followed by a rapid increase （P 〈 0.01）. （2） H89 could completely inhibit TH expression in PC12 cells stimulated by LF-PEF for 10 minutes, while the inhibition rate of U0126 was 53.2%. CONCLUSION： Short-term LF-PEF first promotes then inhibits, while long-term LF-PEF first inhibits then promotes, TH and DA synthesis. LF-PEF stimulation regulates TH expression primarily by activating protein kinase A to regulate DA synthesis.

Adaptive split-step Fourier method for simulating ultrashort laser pulse propagation in photonic crystal fibres

In this paper, the generalized nonlinear Schrodinger equation （GNLSE） is solved by an adaptive split-step Fourier method （ASSFM）. It is found that ASSFM must be used to solve GNLSE to ensure precision when the soliton selffrequency shift is remarkable and the photonic crystal fibre （PCF） parameters vary with the frequency considerably. The precision of numerical simulation by using ASSFM is higher than that by using split-step Fourier method in the process of laser pulse propagation in PCFs due to the fact that the variation of fibre parameters with the peak frequency in the pulse spectrum can be taken into account fully.

THEORY FOR STEP AND PULSE METHOD OF SUPERCRITICAL FLUID CHROMATOGRAPHY

The paper presents a general retention formula for supercritical fluid chromatography at finite concentration. A new chromatographic rate theory based on the fugacities of solutes instead of concentrations is developed, thus relieving the restrictions of linear isothermal distribution and infinite dilution. An expression for retention values of a N component system including solvent is obtained by combining the rate theory with the mass balance equation of solutes in chromatography. It is shown that there are N-1 characteristic retention peaks for N components and the retention time can be calculated directly from the equilibrium properties of the components in both mobile phase and stationary phase at the given conditions. The theory can be reduced to the well known formula for infinite dilution and to the result of step-pulse theory in gas chromatography under low pressure at finite concentration. Supercritical fluid chromatography may eventually prove itself to be a useful reseach tool in the field of phase equilibrium and thermodynamic properties of supercritical fluid system.

Dynamic Response of Stepped Beams Under Impulsive Loading

Aim To analyze dynamic failure of aerospace strutures subjected lateral impulsive loading. Methods Structures were modeled as rigid-perfectly plastic free-free stepped beams. Basic equations of motion un set up for analysis. Results Final pat deformation and rigid motion solutions were determined for a uniform impulsive loading. The critical rupture conditions for a space shuttle and a missile were obtained. Conclusion Failure is possible for aerospace structures under a uniform impulsive loading, but it is mere difficult in space.

Simulation method of near-fault pulse-type ground motion

The two characteristics of near-fault ground motions, i.e., the forward directivity effect and permanent displacement effect, result in long period and large velocity pulse in the velocity time history and large step pulse in the displacement time history. Considering the two effects, a simple expression of continuous function for equivalent velocity pulse time history is presented in this paper. The equivalent pulse model, in which the pulse period, peak velocity and pulse shape are described by five parameters, is highly advantageous to fit and simulate the pulse-type velocity time history. The equivalent pulse model comprises only one low-frequency component while the high-frequency component of a pulse-type earthquake record cannot be considered. Based on 28 records of 11 earthquakes, the pulse frequency of pulse-type records is generally less than 1 Hz. Therefore the low-frequency component and high-frequency component are simulated respectively and combined them together to generate a pulse-type ground motion.

TRULY BALANCED PULSE GENERATOR USING MICROWAVE TRANSISTOR AND SRD

A novel efficient circuit for Ultra-WideBand(UWB) balanced sub-nanosecond monocycle pulse generation is presented.The pulse generator employs wideband bipolar transistor,step recovery diodes,Schottky diodes,and simple charging and discharging circuitry.Simple transient analysis and design of the circuit are presented along with their operating principle.The pulse generator produces truly balanced monocycle pulse with 500 ps pulse-width and 800 mV peak voltage.The generated monocycle pulse also has very symmetrical positive and negative portions and low ringing level.The presented pulse generator can be used as both a transmitter feeding UWB balanced antennas without broadband baluns and a balanced switching pulse generator that used in UWB receiver.

Pulsed Sound Waves in a Compressible Fluid

The propagation along oz of pulsed sound waves made of sequences of elementary unit pulses U (sin τ) where U is the unit step function and τ = kz -ωt is analyzed using the expansion of U (sin τ) and of the Dirac distribution δ (sin τ) in terms of τ-nπ where n is an integer. Their properties and how these pulsed sound waves could be generated are discussed.

Generation of time-dependent ultra-short optical pulse trains in the presence of self-steepening effect

Starting from the extended nonlinear Schrodinger equation in which the self-steepening effect is included, the evolution and the splitting processes of continuous optical wave whose amplitude is perturbed into time related ultra-short optical pulse trains in an optical fibre are numerically simulated by adopting the split-step Fourier algorithm. The results show that the self-steepening effect can cause the characteristic of the pulse trains to vary with time, which is different from the self-steepening-free case where the generated pulse trains consist of single pulses which are identical in width, intensity, and interval, namely when pulses move a certain distance, they turn into the pulse trains within a certain time range. Moreover, each single pulse may split into several sub-pulses. And as time goes on, the number of the sub-pulses will decrease gradually and the pulse width and the pulse intensity will change too. With the increase of the self-steepening parameter, the distance needed to generate time-dependent pulse trains will shorten. In addition, for a large self-steepening parameter and at the distance where more sub-pulses appear, the corresponding frequency spectra of pulse trains are also wider.

The research of the staggered lamination linear pulse launcher

This paper proposes a new type of pulse launcher that applies the principle of linear motors. The stator, viz. gun barrel, of the launcher has a new structure of one segment of iron core consisting of 3 staggered laminations. This structure is helpful in advancing the thrust force per volume. Based on introducing the structure and the working principles, this paper resolves the electromagnetic thrusting force and performs mechanical analysis and experiments on the sample launcher. The research shows that this launcher is simple and brushless structure with brief controlling.

kA级双阶梯脉冲电源方案设计与分析

托卡马克装置预电离过程中,环向磁场应与电子回旋波频率相匹配,NCST装置现有的电子回旋波频率较低,为了让环向场与已有的电子回旋波频率匹配,提出新的环向场线圈电流产生方案,在原方案的磁场线圈平顶电流产生之前增加一个低电流台阶。回顾NCST球形托卡马克装置环向场线圈电源的原有方案后,设计了全控型和半控型两种方案,从电压电流的高次谐波、电流的可控性和纹波、改动成本和安装便捷性四方面对比两个方案的优缺点,最终选定半控型改造方案。根据现场条件制作电源改造柜,尽量减少对原有电源柜的改动。实际测试结果显示,两个电流台阶衔接正常,低电流台阶宽度可调、幅值可调,满足改造要求。

基于台阶声光调Q外腔泵浦MgO:PPLN光参量振荡器的3.4μm中红外脉冲串激光器

本文报道了一种台阶声光调Q外腔泵浦MgO:PPLN光参量振荡器的3.4μm中红外脉冲串激光器.建立了基频台阶声光调Q理论模型,模拟了不同台阶调Q间隔时光子数密度随时间变化趋势,获得了台阶信号最优触发时间,确定了台阶声光调Q获得脉冲串激光输出的可能性.根据理论模拟设计台阶信号触发时间,并应用于台阶声光调Q外腔泵浦MgO:PPLN光参量振荡器的中红外脉冲串激光器实验中,在每个重复周期内声光Q开关分三次开启,获得了单脉冲包络含三个子脉冲的3.4μm中红外脉冲串激光输出.脉冲包络内子脉冲间隔为5μs,最窄脉宽为12.8 ns,脉冲包络重频为20 kHz,理论和是实验中均发现脉冲包络内子脉冲宽度逐渐增大.在最大平均输出功率为1.08 W时,1064 nm基频光与3.4μm参量光的光-光转换效率为10.05%,光束质量因子M 2为2.01.

一种可调延时超窄脉冲触发序列产生技术

为了实现高频信号在欠采样条件下的波形重构,递进延时超窄触发信号的产生成为顺序等效采样技术用于触发取样系统进行高频信号采样的关键。为此,设计了一种可编程延时触发序列产生及调理电路,在FPGA数字电路的控制下,通过计数器与延时模块产生可调延时触发序列,利用阶跃恢复二极管特性对产生的触发序列进行调理,产生一种延时步进可调、边沿极窄的脉冲信号。通过对该电路进行测试,结果表明,输出脉冲信号步进范围0~-2.4 ns可调,分辨率可达1 ps,且边沿跳变时间可以达到120 ps内,幅度可达到8 V左右。该延迟脉冲和调理电路可应用于通信、雷达等信号探测设备中,对于高频信号的获取与分析具有重要意义。

主轴划痕对轴承系统抵御多种载荷冲击的动态性能影响

建立了1个考虑主轴划痕、轴瓦变形和沟槽结构的水润滑橡胶轴承混合润滑瞬态模型,研究了划痕对水润滑橡胶轴承在周期性载荷及非周期性载荷作用下的动态行为影响.结果表明:在偏心载荷下,划痕的存在会使得主轴与轴承出现“高温布朗运动”般的反复碰撞现象,且划痕深度及宽度的增大均会延长启动过程中的微凸体接触时间,严重降低水润滑轴承寿命;在受到脉冲载荷后,划痕会使轴心的运动范围变大,导致轴承与主轴更容易发生碰摩,降低轴承抵抗脉冲载荷冲击的能力;深划痕及宽划痕时,水润滑轴承受到阶跃载荷干扰会从弹流润滑状态进入混合润滑状态.主轴表面出现划痕使轴承系统遭遇外载荷干扰时的自动抵御能力退化.

基于变步长自适应降噪核脉冲信号分析的算法优化研究

针对提高核脉冲信号信噪比这一需求,本文以小波算法为基础,创建了新的阈值函数处理方法,可以满足核脉冲信号降噪基本需求;并进一步提出了一种新的优化变步长自适应非平稳信号降噪算法EVSL,实现核脉冲信号分析。对模拟核信号与原始测量信号同步处理后,与现有算法进行指标对比,结果表明EVSL算法能兼顾收敛速度与稳态精度,有效提高信噪比、保留实际有用信息,是一种有效的提升核脉冲信号质量的方法。

基于磁流变胶泥磁电阻效应的转速检测实验研究

转速是评估旋转机械运行状态的一个重要参数,针对转速检测,本文提出一种基于磁流变胶泥磁电阻效应的转速传感器。由旋转磁场单元与敏感单元组成转速传感器的主体结构,利用静态及动态磁电阻实验研究敏感单元的磁电阻特性,从微观结构层面分析了磁电阻效应机理,推出了敏感单元电阻与激励磁场之间的关系;针对传感器的结构特点建立转速实验测试系统,通过直流电桥的输出电压信号表征被测转速;研究表明:软磁颗粒含量及激励磁场的强度都对敏感单元磁电阻效应有显著影响,随着被测转速的增大,传感器输出电压信号峰峰值具有较好的线性衰减特性,以磁流变胶泥为核心敏感材料的转速传感器可实现对中低转速的有效检测。

一种适用于多场景的新型无变压器高降压比变换器

针对传统Buck变换器受最小占空比限制的影响,无法适用于高降压比场合的问题,提出了一种新型无变压器高降压DC-DC变换器。首先,详细地分析了所提变换器的工作模态和开关时序。其次,在控制层面,该变换器应用交错式脉宽调制的开关模式,不但增加了其带载能力,还极大减少了元器件的电压应力。对新型高降压比变换器的工作过程进行分析,同时在理论上进行了参数设计。最后,搭建500/26 V 430 W的样机进行实验,实验结果充分证明了理论的正确性和可行性。

环形铥光纤激光器中台阶形脉冲的产生

研究了基于非线性偏振旋转(NPR)锁模的铥光纤激光器中台阶形脉冲的产生。通过在NPR锁模器件前后各引入一段长度分别为100 m和350 m长的SMF-28光纤以增强腔内非线性和积累负色散,得到底座宽度为50 ns,重复频率为615.6 kHz,信噪比为55 dB的双波长(波长分别为2025 nm和2034 nm)台阶形脉冲。发现仔细调节偏振控制器和改变泵浦功率可以精细调节台阶形脉冲的形态。通过研究发现台阶形脉冲是由两个矩形脉冲组合而成,且前后泵浦对两个矩形脉冲的影响不同,通过改变偏振态能实现能量在前后两个矩形脉冲之间的转化。本实验为研究被动锁模光纤激光器脉冲动力学和脉冲整形提供了良好的实验平台。

Velocity compensation methods for LPRF modulated frequency stepped-frequency(MFSF) radar

In the high speed target environment,there exists serious Doppler effect in the low pulse repetition frequency（LPRF） modulated frequency stepped frequency（MFSF） radar signal.The velocity range of the target is large and the velocity is high ambiguous,so the single method is difficult to satisfy the velocity measurement requirement.For this problem,a novel method is presented,it is a combination of cross-correlation inner frame velocity measurement and range-Doppler coupling velocity measurement.The cross-correlation inner frame method,overcoming the low Doppler tolerance of the cross-correlation between frames,can obtain the coarse velocity of the high speed target,and then the precision velocity can be obtained with the range-Doppler coupling method.The simulation results confirm the method is effective,and also it is well real-time and easy to the project application.