Correction:Making the Complicated Simple:A Minimizing Carrier Strategy on Innovative Nanopesticides

The Nano-Micro Letters(2024)16:193,article by Shangguan et al.,entitled“Making the Complicated Simple:A Minimizing Carrier Strategy on Innovative Nanopesticides”(Nano-Micro Lett.https://doi.org/10.1007/s40820-024-01413-5),was published online on 14 May,2024,with errors.The structural formulas and captions of the three acyl chlorides in Fig.3A were wrong.They should be as shown below.

The Role of Abdominal CT in the Assessment of the Effectiveness of Gastric Lavage after Pesticide Poisoning

Pesticide poisoning is one of the most common diseases in the emergency department, characterized by rapid changes in condition, a high misdiagnosis rate, and a poor prognosis. Measures for early removal of poisons are crucial, and gastric lavage is one of the important measures. Regarding the post-gastric lavage effect, abdominal CT scanning has an important application value in the assessment of the gastric lavage effect after pesticide poisoning.

On-site rapid detection of multiple pesticide residues in tea leaves by lateral flow immunoassay

The application of pesticides (mostly insecticides and fungicides) during the tea-planting process will undoubtedly increase the dietary risk associated with drinking tea. Thus, it is necessary to ascertain whether pesticide residues in tea products exceed the maximum residue limits. However, the complex matrices present in tea samples comprise a major challenge in the analytical detection of pesticide residues. In this study, nine types of lateral flow immunochromatographic strips (LFICSs) were developed to detect the pesticides of interest (fenpropathrin, chlorpyrifos, imidacloprid, thiamethoxam, acetamiprid, carbendazim, chlorothalonil, pyraclostrobin, and iprodione). To reduce the interference of tea substrates on the assay sensitivity, the pretreatment conditions for tea samples, including the extraction solvent, extraction time, and purification agent, were optimized for the simultaneous detection of these pesticides. The entire testing procedure (including pretreatment and detection) could be completed within 30 min. The detected results of authentic tea samples were confirmed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS), which suggest that the LFICS coupled with sample rapid pretreatment can be used for on-site rapid screening of the target pesticide in tea products prior to their market release.

Coordination of distinctive pesticide adjuvants and atomization nozzles on droplet spectrum evolution for spatial drift reduction

Pesticide adjuvants,as crop protection products,have been widely used to reduce drift loss and improve utilization efficiency by regulating droplet spectrum.However,the coordinated regulation mechanisms of adjuvants and nozzles on droplet spectrum remain unclear.Here,we established the relationship between droplet spectrum evolution and liquid atomization by investigating the typical characteristics of droplet diameter distribution near the nozzle.Based on this,the regulation mechanisms of distinctive pesticide adjuvants on droplet spectrum were clarified,and the corresponding drift reduction performances were quantitively evaluated by wind tunnel experiments.It shows that the droplet diameter firstly shifts to the smaller due to the liquid sheet breakup and then prefers to increase caused by droplet interactions.Reducing the surface tension of sprayed liquid facilitates the uniform liquid breakup and increasing the viscosity inhibits the liquid deformation,which prolong the atomization process and effectively improve the droplet spectrum.As a result,the drift losses of flat-fan and hollow cone nozzles are reduced by about 50%after adding organosilicon and vegetable oil adjuvants.By contrast,the air induction nozzle shows a superior anti-drift ability,regardless of distinctive adjuvants.Our findings provide insights into rational adjuvant design and nozzle selection in the field application.

Evaluation of Agricultural Contamination Level by Heavy Metals and Pesticides in Sediments of Rivers and Water Bodies for Shrimp Fishing in the Lower Ouémé: Case of the Aguigadji, Ahlan and Sele Stations in Benin

This study assessed sediment contamination by heavy metals and pesticide active ingredients linked to chemical inputs used in agricultural activities in the lower Ouémé. Pesticide residues from the organochlorine, pyrethroid and organophosphorus families were investigated by gas chromatography, and heavy metals (Cd, Pb, As, Ni, Zn, Fe, Mg, Cr and Hg) by atomic absorption spectrophotometry. The metallic pollution indices, the contamination factor (CF) and the ecological risk index were calculated. The results revealed 8 active ingredients in the rainy season and 9 in the dry season. Glyphosate was the active ingredient with the highest concentration at all stations, 9.65 ± 0.84 mg/kg recorded in the dry season at the Aguigadji station. All glyphosate values were above the EQS. DDT, Atrazine and Endosulfan also showed high concentrations in the dry and rainy seasons. Emamectin, Abamectin and Lambda Cyhalothrin also showed high concentrations in the dry season at Aguigadji, Ahlan and Sele. Only glyphosate was recorded at the control station (Toho), but in very low concentrations. Lead showed the highest concentrations at all the stations, 265.96 ± 21.02 mg/Kg in the rainy season and 255.38 ± 79.09 mg/Kg in the dry season, all detected at the Aguigadji station and above the EQS. Zn, Ni, Fe, Cu and Cr were all representative in both the dry and rainy seasons at the contaminated stations. Manganese showed high concentrations in the rainy season. Pb showed very high contamination (FC ≥ 6) at the Aguigadji and Ahlan stations and significant contamination (3 ≤ FC 6) at the Sele station in both the rainy and dry seasons. Ni, Hg and Cd, showed either moderate or significant contamination at the contaminated stations. The risk values showed a considerable ecological Ri (190 ≤ Ri < 380) in the rainy season and a moderate ecological Ri (95 ≤ Ri < 190) in the dry season at these contaminated stations.

Resistance Monitoring of Field Populations of Panonychus citri to Three Pesticides

[Objectives]The paper was to investigate the development of Panonychus citri resistance to commonly used pesticides in major citrus producing areas in China,and to screen out new green and efficient agents for its control and prevention.[Methods]The resistance changes of field populations of P.citri to abamectin,pyridaben,and bifenazate in 12 locations across five provinces in China were compared using the leaf disc impregnation method.[Results]P.citri in the tested areas exhibited the most severe resistance to abamectin,with approximately 91.7%of the field populations showing high levels of resistance to abamectin(112.1-560.5 times);50%of P.citri populations exhibited high levels of resistance to pyridaben(123.0-202.7 times),while the remaining populations showed intermediate levels of resistance(25.6-80.3 times);except for the Zhejiang Xiangshan 2019 and Jiangxi Yudu 2019 populations,which exhibited a medium level of resistance to bifenazate,the remaining 10 monitored populations demonstrated a high level of resistance to bifenazate(140.4-686.1 times).[Conclusions]It is advisable to discontinue the use of abamectin and bifenazate due to significant resistance observed in populations of P.citri monitored in 12 locations across 5 provinces.It is recommended to reduce the frequency of pyridaben use and alternate with other acaricides that have different mechanisms of action due to the varying degrees of resistance developed.

Research Progress of QuEChERS Pretreatment Technique in the Detection of Multiple Pesticide Residues

The QuEChERS extraction technique is a pretreatment technique that has been rapidly applied in recent years and is widely used in the field of pesticide residues with many advantages. This technique is based on solid phase extraction (SPE), which uses the interaction between adsorbent and impurities in the matrix to achieve the purpose of purification. The method has easier operation and better purification effect than SPE. In this paper, the research progress of the QuEChERS technique in pesticide residue detection in different fields in recent years and its future development were reviewed, hoping to provide reference for further development and utilization of the QuEChERS technique in pesticide residue detection in the future.

Sensitivity of Commercial Enzyme Inhibition Colorimetric Pesticide Residue Rapid Test Kit to a Variety of Pesticides

[Objectives]To fully understand the quality of commercial enzyme inhibition-colorimetric pesticide residue rapid detection kits,so that they can play a greater role in the detection and supervision of agricultural products.[Methods]The sensitivity of 28 kinds of pesticides was determined by using the commercially available enzyme inhibition colorimetric rapid detection kit with Hendu brand.[Results]There was a significant difference in the sensitivity of the kit to each pesticide,and the kit was more sensitive to dichlorvos among the 28 pesticides tested.The sensitivity to methyl isosalifos,dimethoate,isocarbophos,fenthion and phorate was poor,and the sensitivity to quinalphos was different between 3.0 and 2.5 mL.[Conclusions]The large difference of the sensitivity of the enzyme inhibition-colorimetric rapid detection kit for pesticide residues to different kits is a reason for the false positive and false negative test results of the kit,which needs to be considered by relevant personnel.

Determination of 14 Organophosphorus Pesticide Residues in Mutton by Gel Permeation Chromatography-Gas Chromatography-Mass Spectrometry(GPC-GC-MS)

[Objectives]This study was conducted to purify mutton samples by gel permeation chromatography(GPC).[Methods]Fourteen organophosphorus pesticide residues in samples were qualitatively and quantitatively analyzed by gas chromatography-mass spectrometry(GC-MS)in selective ion scanning mode(SIM).[Results]The organophosphorus pesticide standard solutions showed good linearity in the mass concentration range of 0.1-10.0μg/ml with correlation coefficients(r)not lower than 0.999,and the detection limits(S=3 N)ranged from 0.01 to 0.05 mg/kg.The average recovery values were in the range of 80.2%-99.7%,with relative standard deviations(RSDs,n=3)in the range of 1.8%-6.3%,at the addition levels of 0.5,1.0 and 2.0 mg/kg.[Conclusions]The method is simple,sensitive and accurate,and can be used for the determination of organophosphorus pesticide residues in mutton.

Agricultural Contamination of the Surface Waters of the Upper Ouémé in Benin: The Case of Heavy Metals and Pesticides

Objective: The main objective of this study was to assess the degree of contamination of surface waters by heavy metals and pesticides. Method: To this end, data were collected in December 2022 from four specific sampling stations: Okpara, Térou, Affon and Adjiro. Levels of heavy metals, including cadmium, chromium, copper, iron, mercury, nickel and lead, were measured and subjected to in-depth statistical analysis using graphical summation models. In addition, the concentrations of pesticide active ingredients present in the samples were interpreted and evaluated. The statistical data collected during this study were processed using R software, version 3.5.0. Results: The values obtained at the different stations Okpara, Térou, Affon and Adjiro are respectively Arsenic (2 × 10-4 mg/L;2.2 × 10-1 mg/L;1.2 × 10-4 mg/L;2 × 10-4 mg/L), Cadmium (4.4 × 10-5 mg/L;1.1 × 10-2 mg/L;10-4 mg/L;4 × 10-4 mg/L). Then Copper (7 × 10-4 mg/L;3 × 10-3 mg/L;7 × 10-4 mg/L;1 × 10-4 mg/L), Iron (1.51 mg/L;6.4 × 10-1 mg/L;2.0012 mg/L;2.9 × 10-1 mg/L), Lead (0 mg/L;0 mg/L;1.5 × 10-3 mg/L;1.5 × 10-3 mg/L). Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (Cadmium, Chromium, Copper, Iron, Mercury, nickel and chromium were not detected in surface waters. It is important to note that the values obtained for trace metals (cadmium, chromium, copper, iron, mercury, nickel and lead) were all below the guideline standards set by the WHO in 2006 for uncontaminated surface waters. This indicates that the surface waters of the Upper Ouémé were below acceptable contamination thresholds in terms of heavy metals. However, the presence of pesticide active ingredients such as cyfluthrin, endosulfan-alpha, endosulfan-beta, profenosfos, tihan, atrazine, gala super and glycel clearly indicates that these surface waters are subject to agricultural contamination.

Rapid screening of 420 pesticide residues in fruits and vegetables using ultra high performance liquid chromatography combined with quadrupole-time of flight mass spectrometry

A new method for screening and identification 420 pesticide residues in fruits and vegetables by ultra-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry(UPLC-Q-TOF/MS)were developed.The samples were extracted with acetonitrile/acetic acid(99:1,V/V),and clean-up by SinChERS-Nano(single-step,cheap,effective,rugged,safe,nano)column,determined by UPLC-Q-TOF/MS.The accurate mass database and MS/MS database which contains 420 pesticides were established,the automatic retrieval of detection results was carried on according to the accurate mass,retention time,isotope ratio,ion fragment information,and so on.Method verification was performed on leeks samples.The results showed that 420 pesticides had good linearity in the range of 0.1-100μg/L,and the correlation coeffificients(R2)was greater than 0.990.The limits of detections(LODs)and limits of quantifications(LOQs)of 420 pesticides were in range of 0.05-2.0 and 0.1-5.0μg/L,respectively.The average spike recoveries at 3 levels were 70.1%to 119.7%,and the relative standard deviations(RSD)were lower than 20%(n=6).With this method,a survey of pesticide residues was conducted for 110 samples of 10 different fruits and vegetables,which provided scientific data for ensuring pesticide residue safety of the fruits and vegetables consumed daily by the public.This method was simple,sensitive and accurate,and could be used for rapid screening of 420 pesticide residues in fruits and vegetables.

Regional selection of insecticides and fungal biopesticides to control aphids and thrips and improve the forage quality of alfalfa crops

The efficacies of biological and conventional chemical insecticides against two major insect pests of alfalfa(aphids and thrips)were compared in three sites across China’s alfalfa belt.In addition,the persistence of the residues of chemical insecticides in alfalfa and their influence on the quality of alfalfa hay were examined.Efficacy varied among the different biological and chemical insecticides.The chemical insecticides were significantly more effective than biopesticides in a short time-frame.The efficacy period of biopesticides was significantly longer than that of chemical insecticides,and the corrected mortality rate of aphids in all regions was above 50%at 14 days after application.The analysis of pesticide residues showed that the residual doses of all the pesticides were within the allowed ranges after the safe period.The acid detergent fiber and neutral detergent fiber contents in alfalfa hay were higher and the protein content was lower in chemical insecticide treatments than in biopesticide treatments in Hebei.The relative feeding value of alfalfa hay treated with Metarhizium anisopliae IPP330189 was the highest among the treatments.Compared with chemical insecticides,the yield of alfalfa hay was higher in the biopesticides treatments.Biopesticides show a stronger control effect on insect populations and also a better improvement in the quality of alfalfa hay than chemical insecticides.This study provides a basis for exploring and developing a comprehensive control regime for alfalfa insect pests in the different alfalfa-growing regions in China,and for reducing chemical insecticide usage and improving forage quality.

Farmers’precision pesticide technology adoption and its influencing factors:Evidence from apple production areas in China

The research aimed to understand farmers’willingness to adopt(WTA)and willingness to pay(WTP)for precision pesticide technologies and analyzed the determinants of farmers’decision-making.We used a two-stage approach to consider farmers’WTA and WTP for precision pesticide technologies.A survey of 545 apple farmers was administered in Bohai Bay and the Loess Plateau in China.The data were analyzed using the double-hurdle model.The results indicated that 78.72%of respondents were willing to apply precision pesticide technologies provided by service organizations such as cooperatives and dedicated enterprises,and 69.72%were willing to buy the equipment for using precision pesticide technologies.The results of the determinant analysis indicated that farmers’perceived perceptions,farm scale,cooperative membership,access to digital information,and availability of financial services had significant and positive impacts on farmers’WTA precision pesticide technologies.Cooperative membership,technical training,and adherence to environmental regulations increased farmers’WTP for precision pesticide technologies.Moreover,nonlinear relationships between age,agricultural experience,and farmers’WTA and WTP for precision pesticide technology services were found.

Recognition mechanism and sequence optimization of organophosphorus pesticides aptamers for better monitoring contaminations in food

Aptamers as a kind of biological recognition element have shown great potential in monitoring and the rapid quantification of organophosphorus pesticides(OPPs). However, molecules of OPPs are structurally similar and original aptamers selected by systematic evolution of ligands by exponential enrichment are usually long-chain bases, which hamper the further application under OPPs-aptamer recognition. The aim of the research was to develop a new strategy to design oligonucleotide sequences for binding OPPs by combination of experimental and molecular modeling methods. 3D models of aptamers binding OPPs were constructed, and binding energy and the most probable binding site for the OPPs were then determined by molecular docking, and the binding sites were further confirmed by the results of 2-AP replaced experiments. Based on the docking results, a new aptamer for detection 4 representative OPPs with only 29 bases was designed by reasonable truncation and mutation of the reported aptamer(named S4-29). The interaction between this new aptamer and OPPs were analyzed by molecular docking, microscale thermophoresis, circular dichroism and fluorometric analysis. The results revealed that the new aptamer exhibit more superior recognition performance to OPPs, which can be promote the monitoring ability of OPPs contaminations in food.

Detection and Analysis of Pesticide Residues in Chinese Chives and Risk Assessment of Dietary Intake

[Objectives]This study was conducted to understand the status of pesticide residues and dietary intake risk of Chinese chives in Tangshan area. [Methods] Sixty eight kinds of pesticide residues in 415 Chinese chive samples collected from Tangshan area were qualitatively and quantitatively determined by high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS) and gas chromatography(GC) in 2020. [Results] The results showed that 41 kinds of pesticide residues were detected in the 415 Chinese chive samples, and the detection rate was 69.4%(288/415), and there was a combination of pesticides in many samples. According to the National Food Safety Standard―Maximum Residue Limits of Pesticides in Food(GB 2763-2019), the residues of 12 pesticides exceeded the maximum residue limits(MRLs), and the unqualified rate was 38.07%(158/415). The highest detection rate of clothianidin was 41.20%(171/415), but there was no MRL in GB 2763-2019. The next was procymidone, the detection rate of which was 35.42%(147/415), and the over-standard rate was 30.12%(125/415). Forbidden and restricted pesticides were detected in some samples. According to the dietary exposure risk assessment, the NEDI/ADI values were all less than 1 and the intake risk was within acceptable range. In Tangshan area, the types of pesticides used in Chinese chive production are complex, and there are risks of multi-residue pollution and use of banned and restricted pesticides and unregistered pesticides. It is suggested that routine monitoring of pesticide residues and management of pesticide use should be strengthened to ensure the quality and safety of agricultural products. [Conclusions] This study provides a theoretical basis for the safe production of Chinese chive and the standardized and rational use of pesticides.

Application of Mass Spectrometry (MS)-coupled Techniques in Pesticide Residue Detection

Pesticide residue detection is an important work to ensure the quality safety of agricultural products.In the process of agricultural production,in order to prevent and control agricultural diseases and pests,a certain amount of pesticides need to be used.However,if pesticides are used excessively,there will be certain pesticide residues in crops and related products.Therefore,it is necessary to do a good job in pesticide residue detection.The gas chromatography-mass spectrometry(GC-MS)and liquid chromatography-mass spectrometry(LC-MS)detection methods have good results and can effectively detect pesticide residues in related products.This paper reviewed and analyzed the application of GC-MS and LC-MS in pesticide residue detection,and proposed optimization measures based on practical experience,hoping to provide reference for relevant scholars.

Q-Learning-Based Pesticide Contamination Prediction in Vegetables and Fruits

Pesticides have become more necessary in modern agricultural production.However,these pesticides have an unforeseeable long-term impact on people's wellbeing as well as the ecosystem.Due to a shortage of basic pesticide exposure awareness,farmers typically utilize pesticides extremely close to harvesting.Pesticide residues within foods,particularly fruits as well as veggies,are a significant issue among farmers,merchants,and particularly consumers.The residual concentrations were far lower than these maximal allowable limits,with only a few surpassing the restrictions for such pesticides in food.There is an obligation to provide a warning about this amount of pesticide use in farming.Previous technologies failed to forecast the large number of pesticides that were dangerous to people,necessitating the development of improved detection and early warning systems.A novel methodology for verifying the status and evaluating the level of pesticides in regularly consumed veggies as well as fruits has been identified,named as the Hybrid Chronic Multi-Residual Framework(HCMF),in which the harmful level of used pesticide residues has been predicted for contamination in agro products using Q-Learning based Recurrent Neural Network and the predicted contamination levels have been analyzed using Complex Event Processing(CEP)by processing given spatial and sequential data.The analysis results are used to minimize and effectively use pesticides in the agricultural field and also ensure the safety of farmers and consumers.Overall,the technique is carried out in a Python environment,with the results showing that the proposed model has a 98.57%accuracy and a training loss of 0.30.

Phytosanitary Practices and Pesticide Levels in Fresh and Dried Mangoes Produced in Burkina Faso

Pesticides are used to control fruit flies and other potential crop pests, particularly mango in Burkina Faso. Pesticides are a potential health hazard for users and consumers and are a major constraint to the export and commercialization of mangoes. The objective of this study was to evaluate phytosanitary practices in orchards and pesticide residue levels in four varieties of fresh and dried mangoes (Amelie, Brooks, Kents and Lippens) produced in four localities in Burkina Faso in order to determine their health impact on consumers. Surveys on pesticide use and monitoring of phytosanitary practices were carried out among 16 orchard owners in the localities of Bobo-Dioulasso, Orodara, Toussiana and Banfora, a high mango production area. Also, a total of 120 samples, including 60 samples of fresh mangoes and dried 60 samples of dried mango were collected. A multi-residue method was developed to detect pesticide levels in the collected samples by gas chromatography with a micro-electron capture detector. The study revealed the use of unauthorized pesticides, often specifically for other crops, and a monitoring trapping network in the orchards against insects. 34 chemical pesticides were detected in 120 samples of all mango varieties collected. Pesticide residues were detected in 72% of the samples, and of the positive samples, 48.52% contained concentrations above the maximum residue limit permissibility. Organochlorine pesticide residues were present in fresh mangoes (60.5%) and a total of fifteen active compounds were detected in dried mangoes. The very poor management and use of pesticides found in these orchards could pose a threat to the productivity of natural ecosystems and the health of producers, processors and consumers. Awareness raising and training of producers on the knowledge of the risks linked to the use of pesticides and good practices are necessary to preserve the health of all.

Potential Risks to the Environment as a Result of Pesticide Handling in the Nanumba-North Municipality, Ghana

The present study deduced the potential risks to the environment as a result of pesticide handling in the Nanumba-North Municipality of the Northern Region of Ghana. Cluster sampling was used to select 30 communities from Nanumba-North Municipality. Simple random sampling and purposive sampling were used respectively to select 7 households from each community and one farmer from each household, giving a sample size of 210 farmers. The instrument used for the study was a questionnaire of respondents. The quantitative data obtained were analyzed using frequencies and percentages. The study concluded that 11 types of pesticides are commonly used by the farmers on their fields, with atrazine (22%) being the most commonly used pesticide which is an herbicide, and deltamethrin (1%) was the least used pesticide which is an insecticide. The study, therefore, recommends that appropriate authorities in the area should inculcate means to enlighten farmers on the best way of pesticide utilization that can beef up the ambition of sustainable agricultural production and desirable environmental conditions.

Effect of Particle Size and Pesticide Contamination on Preference and Ingestion Rates by the Tropical Freshwater Shrimp Xiphocaris elongata

In tropical streams, freshwater shrimp are essential to preserve the structure and function of lotic ecosystems. Shredder shrimp play a fundamental role in organic matter decomposition because these feed on detritus. In addition, they are especially important organisms as they connect all trophic levels at food webs. In natural environments, decaying leaf material may accumulate contaminants, including insecticides and herbicides. At these, shredder shrimp can be exposed to these contaminants through ingestion of leaf litter material. The objectives of this study were to evaluate if the shredder shrimp Xiphocaris elongata display a preference for feeding on different plant species and leaf size areas while also assessing their consumption of leaves contaminated with pesticides. We also evaluated acetylcholinesterase (AChE) activity as a possible biomarker of pesticide contamination using an immunofluorescence and microscopy imaging approach. Our results revealed that the leaf area and plant species more appropriate for future toxicological studies is Spathodea campanulata leaves, with a leaf area of 0.65 cm2. This study also showed that sublethal concentrations of malathion and permethrin in leaves seem to have a significant effect on the ingestion rates of X. elongata, which suggests that the presence of these contaminants influenced feeding behavior. Immunofluorescence in cephalothorax ganglia showed a decline in AChE activity when the sublethal dose of malathion and permethrin increased. The observed results suggest that AChE activity can be used as a biomarker to detect and assess permethrin and malathion exposure on shredder shrimp.