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Performance Assessment of a Real PV System Connected to a Low-Voltage Grid 被引量:1
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作者 Gaber Magdy Mostafa Metwally +1 位作者 Adel A.Elbaset Esam Zaki 《Energy Engineering》 EI 2024年第1期13-26,共14页
The generation of photovoltaic(PV)solar energy is increasing continuously because it is renewable,unlimited,and clean energy.In the past,generation systems depended on non-renewable sources such as oil,coal,and gas.Th... The generation of photovoltaic(PV)solar energy is increasing continuously because it is renewable,unlimited,and clean energy.In the past,generation systems depended on non-renewable sources such as oil,coal,and gas.Therefore,this paper assesses the performance of a 51 kW PV solar power plant connected to a low-voltage grid to feed an administrative building in the 6th of October City,Egypt.The performance analysis of the considered grid-connected PV system is carried out using power system simulator for Engineering(PSS/E)software.Where the PSS/E program,monitors and uses the power analyzer that displays the parameters and measures some parameters such as current,voltage,total power,power factor,frequency,and current and voltage harmonics,the used inverter from the type of grid inverter for the considered system.The results conclude that when the maximum solar radiation is reached,the maximum current can be obtained from the solar panels,thus obtaining the maximum power and power factor.Decreasing total voltage harmonic distortion,a current harmonic distortion within permissible limits using active harmonic distortion because this type is fast in processing up to 300 microseconds.The connection between solar stations and the national grid makes the system more efficient. 展开更多
关键词 low-voltage grid photovoltaic(PV)system total harmonic distortion grid-connected PV system
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Review and prospects on the low-voltage Na_(2)Ti_(3)O_(7) anode materials for sodium-ion batteries
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作者 Jun Dong Yalong Jiang +3 位作者 Ruxing Wang Qiulong Wei Qinyou An Xiaoxing Zhang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2024年第1期446-460,I0011,共16页
Due to its low cost and natural abundance of sodium,Na-ion batteries(NIBs)are promising candidates for large-scale energy storage systems.The development of ultralow voltage anode materials is of great significance in... Due to its low cost and natural abundance of sodium,Na-ion batteries(NIBs)are promising candidates for large-scale energy storage systems.The development of ultralow voltage anode materials is of great significance in improving the energy density of NIBs.Low-voltage anode materials,however,are severely lacking in NIBs.Of all the reported insertion oxides anodes,the Na_(2)Ti_(3)O_(7) has the lowest operating voltage(an average potential of 0.3 V vs.Na^(+)/Na)and is less likely to deposit sodium,which has excellent potential for achieving NIBs with high energy densities and high safety.Although significant progress has been made,achieving Na_(2)Ti_(3)O_(7) electrodes with excellent performance remains a severe challenge.This paper systematically summarizes and discusses the physicochemical properties and synthesis methods of Na_(2)Ti_(3)O_(7).Then,the sodium storage mechanisms,key issues and challenges,and the optimization strategies for the electrochemical performance of Na_(2)Ti_(3)O_(7) are classified and further elaborated.Finally,remaining challenges and future research directions on the Na_(2)Ti_(3)O_(7) anode are highlighted.This review offers insights into the design of high-energy and high-safety NIBs. 展开更多
关键词 Sodium-ion batteries low-voltage anode materials Na_(2)Ti_(3)O_(7) Electrochemical performances Electrochemical mechanism
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A Novel Sampling Switch Suitable for Low-Voltage Analog-to-Digital Converters
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作者 彭云峰 周锋 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2006年第8期1367-1372,共6页
A novel, highly linear sampling switch suitable for low-voltage operation is proposed. This switch not only eliminates the nonlinearity introduced by gate-source voltage variation, but also reduces the nonlinearity re... A novel, highly linear sampling switch suitable for low-voltage operation is proposed. This switch not only eliminates the nonlinearity introduced by gate-source voltage variation, but also reduces the nonlinearity resuiting from threshold voltage variation, which has not been accomplished in earlier low-voltage sampling switches. This is achieved by adopting a replica transistor with the same threshold voltage as the sampling transistor. The effectiveness of this technique is demonstrated by a prototype design of a sampling switch in 0. 35μm. The proposed sampling switch achieves a spurious free dynamic range of 111dB for a 0. 2MHz, 1.2Vp-p input signal, sampled at a rate of 2MS/s,about 18dB over the Bootstrapped switch. Also, the on-resistance variation is reduced by 90%. This method is especially useful for low-voltage, high resolution ADCs, which is a hot topic today. 展开更多
关键词 sampling switch NONLINEARITY low-voltage analog-to-digitalconverter switched-capacitor circuits
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TRIP980钢的组织性能调控与动态本构模型
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作者 汪洪波 孙巧妍 +3 位作者 贾大伟 周键 周书杰 钟祥 《塑性工程学报》 CAS CSCD 北大核心 2024年第7期168-174,共7页
设计了一种相变诱导塑性(TRIP)钢,研究了贝氏体等温温度对力学性能和微观组织的影响。针对最优工艺,研究了10^(-4)~10^(3) s^(-1)应变速率范围内的动态力学性能,构建并修正了动态本构数学模型。结果表明:当贝氏体等温温度为380℃时,实... 设计了一种相变诱导塑性(TRIP)钢,研究了贝氏体等温温度对力学性能和微观组织的影响。针对最优工艺,研究了10^(-4)~10^(3) s^(-1)应变速率范围内的动态力学性能,构建并修正了动态本构数学模型。结果表明:当贝氏体等温温度为380℃时,实验钢具有最优的静态力学性能(应变速率10^(-3)s^(-1)),屈服强度达到705 MPa,抗拉强度达到1005 MPa,伸长率为16%,强塑积达到了1.6 GPa·%。应变速率的变化对实验钢的硬化行为和TRIP效应的发生有明显的影响。当应变速率达到10^(3) s^(-1)时,屈服强度和抗拉强度分别达到了815和1145 MPa,分别提高了12.4%和13.9%。经A4系数修正后的动态本构数学模型表明,R值和eAARE的平均值分别达到0.9895和27.5%,具有较精确的预测精度。 展开更多
关键词 trip 微观组织 性能调控 动态本构模型
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Design of Low-Voltage Low Noise Amplifiers with High Linearity 被引量:2
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作者 曹克 杨华中 汪蕙 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2004年第11期1364-1369,共6页
A CMOS radio frequency low noise amplifier with high linearity and low operation voltage of less than 1.0V is presented.In this circuit,an auxiliary MOSFET in the triode region is used to boost the linearity.Simulatio... A CMOS radio frequency low noise amplifier with high linearity and low operation voltage of less than 1.0V is presented.In this circuit,an auxiliary MOSFET in the triode region is used to boost the linearity.Simulation shows that this method can boost the input-referred 3rd-order intercept point with much less power dissipation than that of traditional power/linearity tradeoff solution which pays at least 1dB power for 1dB linearity improvement.It is also shown that the size of the common-gate PMOS transistor needs to be optimized to reduce its loaded input impedance so as not to degrade the linearity due to high voltage gain at its source terminal.The simulation is carried out with TSMC 0.18μm RF CMOS technology and SpectreRF. 展开更多
关键词 low-voltage radio frequency CMOS low noise amplifier LINEARITY
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Coordinated planning for flexible interconnection and energy storage system in low-voltage distribution networks to improve the accommodation capacity of photovoltaic 被引量:2
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作者 Jiaguo Li Lu Zhang +1 位作者 Bo Zhang Wei Tang 《Global Energy Interconnection》 EI CSCD 2023年第6期700-713,共14页
The increasing proportion of distributed photovoltaics(DPVs)and electric vehicle charging stations in low-voltage distribution networks(LVDNs)has resulted in challenges such as distribution transformer overloads and v... The increasing proportion of distributed photovoltaics(DPVs)and electric vehicle charging stations in low-voltage distribution networks(LVDNs)has resulted in challenges such as distribution transformer overloads and voltage violations.To address these problems,we propose a coordinated planning method for flexible interconnections and energy storage systems(ESSs)to improve the accommodation capacity of DPVs.First,the power-transfer characteristics of flexible interconnection and ESSs are analyzed.The equipment costs of the voltage source converters(VSCs)and ESSs are also analyzed comprehensively,considering the differences in installation and maintenance costs for different installation locations.Second,a bilevel programming model is established to minimize the annual comprehensive cost and yearly total PV curtailment capacity.Within this framework,the upper-level model optimizes the installation locations and capacities of the VSCs and ESSs,whereas the lower-level model optimizes the operating power of the VSCs and ESSs.The proposed model is solved using a non-dominated sorting genetic algorithm with an elite strategy(NSGA-II).The effectiveness of the proposed planning method is validated through an actual LVDN scenario,which demonstrates its advantages in enhancing PV accommodation capacity.In addition,the economic benefits of various planning schemes with different flexible interconnection topologies and different PV grid-connected forms are quantitatively analyzed,demonstrating the adaptability of the proposed coordinated planning method. 展开更多
关键词 low-voltage distribution network Photovoltaic accommodation Flexible interconnection Energy storage system Bilevel programming
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Low-Voltage Analysis of Distribution Network Software Design and Application 被引量:2
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作者 Mingqiu Du Yan Li +1 位作者 Chunfang Liu Tangjie Liang 《Energy and Power Engineering》 2017年第4期183-188,共6页
With the rapid development of urban economy, distribution network construction of city is lagging behind the increment of load, and low-voltage problem has become the main complaints of the power users. Based on Pytho... With the rapid development of urban economy, distribution network construction of city is lagging behind the increment of load, and low-voltage problem has become the main complaints of the power users. Based on Python [1] scripting language, this paper develops a piece of software which can provide a friendly human-machine experience for the user, calculate the voltage of the power distribution area and propose corresponding control measures, then the paper takes the actual power distribution area to verify its effectiveness. 展开更多
关键词 low-voltage ANALYSIS DISTRIBUTION NETWORK PYTHON
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Low-voltage Electromagnetic Compaction of Metal and Ceramic Powders 被引量:1
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作者 孟正华 黄尚宇 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2007年第4期714-717,共4页
Low-voltage electromagnetic compaction (EMC) was used to compact metal powders (Cu) and ceramic powders (TiO2) in the indirect way. It was found that the density of the metal powder parts compacted by low-voltag... Low-voltage electromagnetic compaction (EMC) was used to compact metal powders (Cu) and ceramic powders (TiO2) in the indirect way. It was found that the density of the metal powder parts compacted by low-voltage EMC varied linearly with the discharging voltage in the range investigated. But for ceramic powders, the discharging voltage has an optimal value. Under the value, the density increases as discharging voltage rises, but beyond the value the trend is reverse. The experimental results show that the density of the metal parts decreases gradually along press direction. And the density of the ceramic parts decreases with the advancement of the aspect ratio h/d (height/diameter). In addition, repetitive compaction can improve the density of both metal and ceramic parts and reduce the effects of aspect ratio on the density. 展开更多
关键词 POWDER low-voltage electromagnetic compaction DENSITY
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A low-voltage and energy-efficient full adder cell based on carbon nanotube technology 被引量:1
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作者 Keivan Navi Rabe'e Sharifi Rad +1 位作者 Mohammad Hossein Moaiyeri Amir Momeni 《Nano-Micro Letters》 SCIE EI CAS 2010年第2期114-120,共7页
Scaling problems and limitations of conventional silicon transistors have led the designers to exploit novel nano-technologies. One of the most promising and feasible nano-technologies is CNT(Carbon Nanotube) based tr... Scaling problems and limitations of conventional silicon transistors have led the designers to exploit novel nano-technologies. One of the most promising and feasible nano-technologies is CNT(Carbon Nanotube) based transistors. In this paper, a high-speed and energy-efficient CNFET(Carbon Nanotube Field Effect Transistor) based Full Adder cell is proposed for nanotechnology. This design is simulated in various supply voltages, frequencies and load capacitors using HSPICE circuit simulator. Significant improvement is achieved in terms of speed and PDP(Power-Delay-Product) in comparison with other classical and state-of-the-art CMOS and CNFET-based designs, existing in the literature. The proposed Full Adder can also drive large load capacitance and works properly in low supply voltages. 展开更多
关键词 CNFET low-voltage Full-Adder Minority-Function NANOTECHNOLOGY
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Numerical Analysis of the Arc Plasma in a Simplified Low-voltage Circuit Breaker Chamber with Ferromagnetic Materials 被引量:1
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作者 吴翊 荣命哲 +1 位作者 杨茜 胡光霞 《Plasma Science and Technology》 SCIE EI CAS CSCD 2005年第4期2977-2981,共5页
This paper is devoted to the simulation of the arc plasma in a simplified low-voltage circuit breaker chamber. Based on a group of coupled governing equations, a three-dimensional (3-D) arc plasma model is built and... This paper is devoted to the simulation of the arc plasma in a simplified low-voltage circuit breaker chamber. Based on a group of coupled governing equations, a three-dimensional (3-D) arc plasma model is built and solved by a modified commercial code. Firstly, this paper presents a solution of the stationary state of the arc plasma and discusses the distribution of some parameters throughout the chamber. Secondly, with the ferromagnetic materials included, the balance of the stationary state is broken and a transient course is calculated. In light of the simulation results, the temperature distribution sequence, the arc motion and the plasma jet are then described and analyzed in detail. 展开更多
关键词 arc plasma low-voltage SIMULATION
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INVESTIGATION ON THE DENSITY UNIFORMITY OF PRESSED PARTS BY LOW-VOLTAGE ELECTROMAGNETIC COMPACTION 被引量:1
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作者 S.Y.Huang Z.H.Meng +2 位作者 Y.C.Li B.Zhang W.Ouyang 《Acta Metallurgica Sinica(English Letters)》 SCIE EI CAS CSCD 2004年第6期805-811,共7页
Powder metallurgy is an efficient approach to fabricate varieties of high performance structure materials, function materials and special materials working under limited conditions. Research and development of new eff... Powder metallurgy is an efficient approach to fabricate varieties of high performance structure materials, function materials and special materials working under limited conditions. Research and development of new efficient technology to form high-density, high-performance and net shape parts is a key to widen application and development of powder materials. Recently, the low-voltage electromagnetic compaction (EMC) has been used by present authors to compacted copper, tin, aluminum powders and the products with 99% relative density have been acquired. In this work, the research has been extended to investigation on the density uniformity of pressed parts. The analysis results show that the density of the part compacted by low-voltage EMC decreases gradually in press direction as static compaction. But it is higher and more homogeneous. The density of the top part increases gradually from the center to the outer, which is just reversal of the bottom part. In some extent, the higher the discharging voltage is, the higher the density is and the more homogeneous the distribution is. In addition, repetitive compaction can improve the density of powder parts and the distribution uniformity. 展开更多
关键词 POWDER low-voltage electromagnetic compaction density uniformity density distribution
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Research of Three-Phase Unbalanced Treatment in Low-Voltage Distribution Network Based on New Commutation Switch 被引量:1
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作者 Xue Liu Jun Jia Jian Wang 《World Journal of Engineering and Technology》 2019年第4期10-17,共8页
Low-voltage distribution systems in our country are mostly used in agricultural loads and household loads. The value and using time of these kinds of loads are uncontrollable, which lead to the three-phase imbalance i... Low-voltage distribution systems in our country are mostly used in agricultural loads and household loads. The value and using time of these kinds of loads are uncontrollable, which lead to the three-phase imbalance in low-voltage distribution system, and seriously affect the quality of power supply. A new type of the commutation system and an improved quantum genetic algorithm (IQGA) are proposed in the paper. At last, the rationality and the efficiency of the method are verified by a practical example. 展开更多
关键词 low-voltage DISTRIBUTION Network THREE-PHASE UNBALANCE COMMUTATION SWITCH IQGA
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The Low-Voltage Electromagnetic Compaction of Powder Materials
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作者 吴彦春 《Journal of Wuhan University of Technology(Materials Science)》 SCIE EI CAS 2002年第4期39-43,共5页
The low-voltage-electromagnetic forming was applied to powder compaction.A series of experiments was performed to compact aluminum,copper and tin powders in an indirect working way.Having compacted high-density powder... The low-voltage-electromagnetic forming was applied to powder compaction.A series of experiments was performed to compact aluminum,copper and tin powders in an indirect working way.Having compacted high-density powder parts successfully,the authors analyzed the effects of voltage,capacitance,friction,compaction times,powder size and other factors on the densities of compacted specimens.The experimental results show that lower voltage but larger capacitance are beneficial to increasing the density and homogeneity of the compacted specimens,if the loading velocity and discharging energy are suitable.The higher the voltage,the greater the percentage of energy consumed by friction.If the equipment energy is limited,the iterative compaction is an efficient way to manufacture homogeneous and high-density powder parts. 展开更多
关键词 powder compaction low-voltage electromagnetic compaction
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Simulation of Low-Voltage Arc Plasma During Contact Opening Progress
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作者 吴翊 荣命哲 +3 位作者 孙志强 王小华 李剑 王建华 《Plasma Science and Technology》 SCIE EI CAS CSCD 2007年第6期649-652,共4页
This paper focuses on the simulation of the low-voltage arc with an opening contact. A controllable experiment setup with a rotating contact is designed to investigate the arc behaviour. Supported by the experiment, t... This paper focuses on the simulation of the low-voltage arc with an opening contact. A controllable experiment setup with a rotating contact is designed to investigate the arc behaviour. Supported by the experiment, the phenomena of arc elongation and commutation in the case of rotating contact are simulated with the dynamic grid technique introduced. Under the given condition of the external magnetic field and the contact rotating velocity, the stagnation and rapid jump of two arc roots are observed by the calculated and experimental arc root displacement. The voltage of arc column can be divided into four phases and its sharp rising progress comes from the increase of the displacement difference between two arc roots in x direction. 展开更多
关键词 arc plasma low-voltage switching devices
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Differences between Fallers by Tripping and Nonfallers in the OSFS Test
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作者 Sohee Shin Shinichi Demura +3 位作者 Tsuneo Watanabe Bateer Shi Tamotsu Yabumoto Toshio Matsuoka 《Advances in Aging Research》 2014年第2期124-129,共6页
This study evaluates the effects of a fall experience caused by tripping during the repetitive stepping movements over an obstacle [obstacle-single leg forward step (OSFS) test]. The study included 147 participants wh... This study evaluates the effects of a fall experience caused by tripping during the repetitive stepping movements over an obstacle [obstacle-single leg forward step (OSFS) test]. The study included 147 participants who were divided into 2 groups: 25 fallers caused by tripping and 122 nonfallers. The subjects were asked to step forward over a 10-cm-high obstacle with 1 leg and then return to their original position, as quickly as possible, and this test was repeated for 5 times. The OSFS test was evaluated in 2 phases: the OSFS-F phase, wherein the participants stepped forward on one leg, and the OSFS-R phase, wherein they returned to their original position. Significant differences were observed in both phases of the OSFS test between the two groups, and the fallers by tripping were significantly inferior to the nonfallers. The area under the curve [AUC;area under the receiver operating characteristic (ROC) curve] was more than 0.63 for all the parameters, which was statistically significant. In conclusion, the fallers by tripping were inferior to the nonfallers in the obstacle step movement. 展开更多
关键词 Fallers by tripping Nonfallers ROC CURVE OSFS TEST
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The effects of fall experience by tripping on the movement of stepping over an obstacle
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作者 Sohee Shin Shinichi Demura +2 位作者 Hiroki Sugiura Yuu Uchida Ning Xu 《Advances in Aging Research》 2013年第2期81-85,共5页
This study examines the effects of fall experience caused by tripping on the movement of stepping over an obstacle. The participants were divided into 3 groups (26 fallers caused by tripping, 24 fallers caused by othe... This study examines the effects of fall experience caused by tripping on the movement of stepping over an obstacle. The participants were divided into 3 groups (26 fallers caused by tripping, 24 fallers caused by other causes, and 145 non-fallers). Participants stepped forward over a 10 cm high obstacle with one leg, and then returned to their original position five times as quickly as possible. The OSFS (obstacle single leg forward step) test was measured in the following 2 phases: the OSFS-F phase, in which participants stepped out on one leg, and the OSFS-R phase, in which they returned it. Significant differences among the three groups were found in all parameters, and the fallers by tripping were significantly inferior to the non-fallers. There were no significant differences between the fallers by other reasons and the non-fallers in all parameters. The fallers by tripping were slower in the obstacle step movement than the non-fallers. 展开更多
关键词 tripping Faller/Non-Faller OSFS TEST
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Enhancement of holding voltage by a modified low-voltage trigger silicon-controlled rectifier structure for electrostatic discharge protection
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作者 陈远康 周远良 +3 位作者 蒋杰 饶庭柯 廖武刚 刘俊杰 《Chinese Physics B》 SCIE EI CAS CSCD 2023年第2期514-518,共5页
A novel structure of low-voltage trigger silicon-controlled rectifiers(LVTSCRs) with low trigger voltage and high holding voltage is proposed for electrostatic discharge(ESD) protection. The proposed ESD protection de... A novel structure of low-voltage trigger silicon-controlled rectifiers(LVTSCRs) with low trigger voltage and high holding voltage is proposed for electrostatic discharge(ESD) protection. The proposed ESD protection device possesses an ESD implant and a floating structure. This improvement enhances the current discharge capability of the gate-grounded NMOS and weakens the current gain of the silicon-controlled rectifier current path. According to the simulation results, the proposed device retains a low trigger voltage characteristic of LVTSCRs and simultaneously increases the holding voltage to 5.53 V, providing an effective way to meet the ESD protection requirement of the 5 V CMOS process. 展开更多
关键词 electrostatic discharge floating n-well low-voltage trigger silicon-controlled rectifier
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Fluid simulation of the pulsed bias effect on inductively coupled nitrogen discharges for low-voltage plasma immersion ion implantation
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作者 Xiao-Yan Sun Yu-Ru Zhang +1 位作者 Xue-Chun Li You-Nian Wang 《Chinese Physics B》 SCIE EI CAS CSCD 2017年第1期290-297,共8页
Planar radio frequency inductively coupled plasmas(ICP) are employed for low-voltage ion implantation processes,with capacitive pulse biasing of the substrate for modulation of the ion energy. In this work, a two-di... Planar radio frequency inductively coupled plasmas(ICP) are employed for low-voltage ion implantation processes,with capacitive pulse biasing of the substrate for modulation of the ion energy. In this work, a two-dimensional(2D) selfconsistent fluid model has been employed to investigate the influence of the pulsed bias power on the nitrogen plasmas for various bias voltages and pulse frequencies. The results indicate that the plasma density as well as the inductive power density increase significantly when the bias voltage varies from 0 V to-4000 V, due to the heating of the capacitive field caused by the bias power. The N+fraction increases rapidly to a maximum at the beginning of the power-on time, and then it decreases and reaches the steady state at the end of the glow period. Moreover, it increases with the bias voltage during the power-on time, whereas the N2-+ fraction exhibits a reverse behavior. When the pulse frequency increases to 25 kHz and40 kHz, the plasma steady state cannot be obtained, and a rapid decrease of the ion density at the substrate surface at the beginning of the glow period is observed. 展开更多
关键词 fluid simulation low-voltage plasma immersion ion implantation N2 inductive discharge
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OFDM-based Low-voltage Powerline High Rate Communication
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作者 张有兵 程时杰 +1 位作者 Joseph Nguimbis 熊兰 《Journal of Donghua University(English Edition)》 EI CAS 2004年第1期13-17,共5页
Based on the experimental results, a simplified model for low-voltage powerline used as a high frequency communication channel is presented. With this model, the Orthogonal Frequency Division Multiplexing (OFDM) based... Based on the experimental results, a simplified model for low-voltage powerline used as a high frequency communication channel is presented. With this model, the Orthogonal Frequency Division Multiplexing (OFDM) based high rate digital communication over low-voltage powerline is analyzed and simulated. The capability of the signal transmission system in overcoming multi-path interference and selection of the system parameters are discussed. And time-domain simulation is carried out to investigate the transmission capability of the OFDM communication system for different mapping schemes and transmission power levels. Simulation results show that it is possible to realize high rate digital communication over low-voltage powerline using OFDM when the transmitted power is large enough. 展开更多
关键词 low-voltage powerline communication OFDM modeling of channel
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TRIP13通过同源重组通路提高肺腺癌细胞的放射抗性
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作者 葛舒童 谷润川 +3 位作者 杨雄涛 许长丹 王诗杰 朱广迎 《中国肺癌杂志》 CAS CSCD 北大核心 2024年第1期1-12,共12页
背景与目的放疗是非小细胞肺癌(non-small cell lung cancer,NSCLC)最常用的治疗手段之一。然而,一部分肿瘤细胞对放射线的不敏感是放疗疗效差、患者预后不良的重要原因之一,探究放射抵抗背后的深层机制是解决这一临床难题的关键。本研... 背景与目的放疗是非小细胞肺癌(non-small cell lung cancer,NSCLC)最常用的治疗手段之一。然而,一部分肿瘤细胞对放射线的不敏感是放疗疗效差、患者预后不良的重要原因之一,探究放射抵抗背后的深层机制是解决这一临床难题的关键。本研究旨在寻找与肺腺癌(lungadenocarcinoma,LUAD)放射抵抗相关的分子,初步经数据库筛选锁定甲状腺素受体结合因子13(thyroid hormone receptor interactor 13,TRIP13)为主要研究对象,并探索TRIP13是否与LUAD的放射抵抗有关及具体机制,以期为临床接受放疗的LUAD患者的联合治疗提供理论依据和潜在靶点。方法选取基因表达综合数据库(Gene Expression Omnibus,GEO)中的GSE18842、GSE19188和GSE33532共3个数据集,借助R 4.1.3软件分别筛选3个数据集中差异表达的基因(|logFC|>1.5,P<0.05),之后使用Venn diagram找出在3个数据集中共有的差异表达基因。随后,借助STRING在线工具和Cytoscape软件,对筛选出来的差异基因进行蛋白质相互作用分析和模块分析,借助Kaplan-Meier Plotter数据库对各基因进行生存预后分析,并确定TRIP13基因作为后续主要研究分子。随后,采用亚致死性剂量照射法对人LUAD细胞系H292进行多次X射线照射,以构建具有放射抗性的细胞系H292DR。采用细胞计数试剂盒-8(cell counting kit-8,CCK-8)实验和克隆形成实验验证H292DR细胞的放射抗性能力。Western blot检测H292细胞和H292DR细胞中TRIP13蛋白的表达水平。使用小干扰RNA(small interfering RNA,siRNA)沉默H292DR细胞中TRIP 13蛋白的表达并进行Western blot检测。观察TRIP13沉默后H292DR细胞的克隆形成能力和迁移能力,随后检测共济失调-毛细血管扩张突变(ataxia telangiectasia mutated,ATM)蛋白等与同源重组密切相关的蛋白的表达水平变化。结果经多个GEO数据集筛选、外部数据集的验证以及生存分析发现,TRIP13在LUAD中高表达,并与接受过放疗的LUAD患者的不良预后有关;并且,TRIP13基因富集分析(gene set enrichment analysis,GSEA)的结果提示,TRIP13可能通过促进放疗后的同源重组修复而与LUAD放射抵抗有密切关联。经实验检测发现,TRIP13的表达在H292DR中上调,而沉默TRIP13后能够增加H292DR细胞对放射线的敏感性。结论TRIP13与接受放疗后的LUAD患者的预后不良有关,可能是通过促进同源重组修复途径来介导LUAD细胞对放射线的抵抗。 展开更多
关键词 肺肿瘤 放射抵抗 trip13蛋白 同源重组
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