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The dorsal root ganglion as a target for neurorestoration in neuropathic pain 被引量:1
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作者 Guillermo Estivill-Torrús Ana Belen Martínez-Padilla +2 位作者 Lourdes Sánchez-Salido Anne Baron-Van Evercooren Beatriz García-Díaz 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第2期296-301,共6页
Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.... Neuropathic pain is a severe and chronic condition widely found in the general population.The reason for this is the extensive variety of damage or diseases that can spark this unpleasant constant feeling in patients.During the processing of pain,the dorsal root ganglia constitute an important region where dorsal root ganglion neurons play a crucial role in the transmission and propagation of sensory electrical stimulation.Furthermore,the dorsal root ganglia have recently exhibited a regenerative capacity that should not be neglected in the understanding of the development and resolution of neuropathic pain and in the elucidation of innovative therapies.Here,we will review the complex interplay between cells(satellite glial cells and inflammatory cells)and factors(cytokines,neurotrophic factors and genetic factors)that takes place within the dorsal root ganglia and accounts for the generation of the aberrant excitation of primary sensory neurons occurring in neuropathic pain.More importantly,we will summarize an updated view of the current pharmacologic and nonpharmacologic therapies targeting the dorsal root ganglia for the treatment of neuropathic pain. 展开更多
关键词 CYTOKINES dorsal root ganglia genetic factors neuropathic pain neurotrophic factors pharmacologic and nonpharmacologic therapies satellite glial cells sensory neurons
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Prediction of cell-cell communication patierns of dorsal root ganglion cells:single-cell RNA sequencing data analysis
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作者 Yanna Lian Cheng Wu +1 位作者 Li Liu Xiangyao Li 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第6期1367-1374,共8页
Dorsal root ganglion neurons transmit peripheral somatic information to the central nervous system,and dorsal root ganglion neuron excitability affects pain perception.Dorsal root ganglion stimulation is a new approac... Dorsal root ganglion neurons transmit peripheral somatic information to the central nervous system,and dorsal root ganglion neuron excitability affects pain perception.Dorsal root ganglion stimulation is a new approach for managing pain sensation.Knowledge of the cell-cell communication among dorsal root ganglion cells may help in the development of new pain and itch management strategies.Here,we used the single-cell RNA-sequencing(scRNA-seq)database to investigate intercellular communication networks among dorsal root ganglion cells.We collected scRNA-seq data from six samples from three studies,yielding data on a total of 17,766 cells.Based on genetic profiles,we identified satellite glial cells,Schwann cells,neurons,vascular endothelial cells,immune cells,fibroblasts,and vascular smooth muscle cells.Further analysis revealed that eight types of dorsal root ganglion neurons mediated proprioceptive,itch,touch,mechanical,heat,and cold sensations.Moreover,we predicted several distinct forms of intercellular communication among dorsal root ganglion cells,including cell-cell contact,secreted signals,extracellular matrix,and neurotransmitter-mediated signals.The data mining predicted that Mrgpra3-positive neurons robustly express the genes encoding the adenosine Adora2b(A2B)receptor and glial cell line-derived neurotrophic factor family receptor alpha 1(GFRα-1).Our immunohistochemistry results confirmed the coexpression of the A2B receptor and GFRα-1.Intrathecal injection of the A2B receptor antagonist PSB-603 effectively prevented histamine-induced scratching behaviour in a dose-dependent manner.Our results demonstrate the involvement of the A2B receptor in the modulation of itch sensation.Furthermore,our findings provide insight into dorsal root ganglion cell-cell communication patterns and mechanisms.Our results should contribute to the development of new strategies for the regulation of dorsal root ganglion excitability. 展开更多
关键词 cell-cell communications CellChat dorsal root ganglion scRNA-Seq database
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Outcomes following minimally invasive dorsal cheilectomy for hallux rigidus:A systematic review
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作者 Katherine Esser James J Butler +5 位作者 Mackenzie Roof Nathaniel P Mercer Michael C Harrington Alan P Samsonov Andrew J Rosenbaum John G Kennedy 《World Journal of Orthopedics》 2024年第6期585-592,共8页
BACKGROUND Cheilectomy of the 1^(st)metatarsophalangeal joint(MTPJ)is one of the most common procedures for the management of hallux rigidus.However,there is no consensus regarding outcomes following minimally invasiv... BACKGROUND Cheilectomy of the 1^(st)metatarsophalangeal joint(MTPJ)is one of the most common procedures for the management of hallux rigidus.However,there is no consensus regarding outcomes following minimally invasive dorsal cheilectomy(MIDC)for the management of hallux rigidus.AIM To evaluate outcomes following MIDC for the management of hallux rigidus.METHODS During November 2023,the PubMed,EMBASE and Cochrane Library databases were systematically reviewed to identify clinical studies examining outcomes following MIDC for the management of hallux rigidus.RESULTS Six studies were included.In total,348 patients(370 feet)underwent MIDC for hallux rigidus at a weighted mean follow-up of 37.9±16.5 months.The distribution of patients by Coughlin and Shurna's classification was recorded in 4 studies as follows:Ⅰ(58 patients,27.1%),Ⅱ(112 patients,52.3%),Ⅲ(44 patients,20.6%).Three studies performed an additional 1^(st)MTPJ arthroscopy and debridement following MIDC.Retained intra-articular bone debris was observed in 100%of patients in 1 study.The weighted mean American orthopedic foot and ankle society score improved from a preoperative score of 68.9±3.2 to a postoperative score of 87.1.The complication rate was 8.4%,the most common of which was persistent joint pain and stiffness.Thirty-two failures(8.7%)were observed.Thirty-three secondary procedures(8.9%)were performed at a weighted mean time of 8.6±3.2 months following the index procedure.CONCLUSION This systematic review demonstrated improvements in subjective clinical outcomes together with a moderate complication rate following MIDC for the management of hallux rigidus at short-term follow-up.A moderate reoperation rate at short-term follow-up was recorded.The marked heterogeneity between included studies and paucity of high quality comparative studies limits the generation of any robust conclusions. 展开更多
关键词 Minimally invasive dorsal cheilectomy Hallux rigidus The first metatarsophalangeal joint Cheilectomy Minimally invasive surgery
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Electroacupuncture Alleviates Memory Deficits in APP/PS1 Mice by Targeting Serotonergic Neurons in Dorsal Raphe Nucleus
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作者 Chao-chao YU Xiao-fei WANG +8 位作者 Jia WANG Chu LI Juan XIAO Xue-song WANG Rui HAN Shu-qin WANG Yuan-fang LIN Li-hong KONG Yan-jun DU 《Current Medical Science》 SCIE CAS 2024年第5期987-1000,共14页
Objective Alzheimer’s disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impair... Objective Alzheimer’s disease(AD)has become a significant global concern,but effective drugs able to slow down AD progression is still lacked.Electroacupuncture(EA)has been demonstrated to ameliorate cognitive impairment in individuals with AD.However,the underlying mechanisms remains poorly understood.This study aimed at examining the neuroprotective properties of EA and its potential mechanism of action against AD.Methods APP/PS1 transgenic mice were employed to evaluate the protective effects of EA on Shenshu(BL 23)and Baihui(GV 20).Chemogenetic manipulation was used to activate or inhibit serotonergic neurons within the dorsal raphe nucleus(DRN).Learning and memory abilities were assessed by the novel object recognition and Morris water maze tests.Golgi staining,western blot,and immunostaining were utilized to determine EA-induced neuroprotection.Results EA at Shenshu(BL 23)and Baihui(GV 20)effectively ameliorated learning and memory impairments in APP/PS1 mice.EA attenuated dendritic spine loss,increased the expression levels of PSD95,synaptophysin,and brain-derived neurotrophic factor in hippocampus.Activation of serotonergic neurons within the DRN can ameliorate cognitive deficits in AD by activating glutamatergic neurons mediated by 5-HT1B.Chemogenetic inhibition of serotonergic neurons in the DRN reversed the effects of EA on synaptic plasticity and memory.Conclusion EA can alleviate cognitive dysfunction in APP/PS1 mice by activating serotonergic neurons in the DRN.Further study is necessary to better understand how the serotonergic neurons-related neural circuits involves in EA-induced memory improvement in AD. 展开更多
关键词 Alzheimer’s disease ELECTROACUPUNCTURE dorsal raphe nucleus HIPPOCAMPUS serotonergic neurons glutamatergic neurons 5-HT1B cognitive impairment chemogenetic manipulation synaptic plasticity
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Transcriptional regulatory network during axonal regeneration of dorsal root ganglion neurons:laser-capture microdissection and deep sequencing 被引量:1
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作者 Li-Li Zhao Tao Zhang +2 位作者 Wei-Xiao Huang Ting-Ting Guo Xiao-Song Gu 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第9期2056-2066,共11页
The key regulators and regeneration-associated genes involved in axonal regeneration of neurons after injury have not been clarified.In high-throughput sequencing,various factors influence the final sequencing results... The key regulators and regeneration-associated genes involved in axonal regeneration of neurons after injury have not been clarified.In high-throughput sequencing,various factors influence the final sequencing results,including the number and size of cells,the depth of sequencing,and the method of cell separation.There is still a lack of research on the detailed molecular expression profile during the regeneration of dorsal root ganglion neuron axon.In this study,we performed lase r-capture microdissection coupled with RNA sequencing on dorsal root ganglion neurons at 0,3,6,and 12 hours and 1,3,and 7 days after sciatic nerve crush in rats.We identified three stages after dorsal root ganglion injury:early(3-12 hours),pre-regeneration(1 day),and regeneration(3-7 days).Gene expression patterns and related function enrichment res ults showed that one module of genes was highly related to axonal regeneration.We verified the up-regulation of activating transcription factor 3(Atf3),Kruppel like factor 6(Klf6),AT-rich inte raction domain 5A(Arid5α),CAMP responsive element modulator(Crem),and FOS like 1,AP-1 transcription factor Subunit(Fosl1) in dorsal root ganglion neurons after injury.Suppressing these transcription factors(Crem,Arid5o,Fosl1 and Klf6) reduced axonal regrowth in vitro.As the hub transcription factor,Atf3 showed higher expression and activity at the preregeneration and regeneration stages.G protein-coupled estrogen receptor 1(Gper1),inte rleukin 12a(Il12α),estrogen receptor 1(ESR1),and interleukin 6(IL6) may be upstream factors that trigger the activation of Atf3 during the repair of axon injury in the early stage.Our study presents the detailed molecular expression profile during axonal regeneration of dorsal root ganglion neurons after peripheral nerve injury.These findings may provide reference for the clinical screening of molecular targets for the treatment of peripheral nerve injury. 展开更多
关键词 Arid5a ATF3 Crem dorsal root ganglion Fosl1 KLF6 laser-capture microdissection NEURON smart-seq2 gene expression profile transcription factor
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Effect of Selective Dorsal Neurectomy on Erectile Function in Rats
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作者 Jin WANG Sen LI +7 位作者 Wen-jia LI Wen-li ZHU Yuan-bin XIA Yao HE You-peng ZHANG Han-qing ZENG Yong-biao CHENG Zhao-hui ZHU 《Current Medical Science》 SCIE CAS 2023年第2期324-328,共5页
Objective This study aimed to investigate the effect of penile selective dorsal neurectomy(SDN)on erectile function in rats.Methods Twelve adult male Sprague-Dawley rats(15 weeks old)were divided into three groups(n=4... Objective This study aimed to investigate the effect of penile selective dorsal neurectomy(SDN)on erectile function in rats.Methods Twelve adult male Sprague-Dawley rats(15 weeks old)were divided into three groups(n=4 per group):in control group,rats received no treatment;in sham group,rats underwent a sham operation;in SDN group,rats underwent SDN with half of the dorsal penile nerve severed.The mating test was performed,and the intracavernous pressure(ICP)assessed six weeks after the surgical treatment.Results At postoperative six weeks,the mating test revealed no significant difference in mounting latency and mounting frequency among the three groups(P>0.05),while the ejaculation latency(EL)was significantly longer and ejaculation frequency(EF)lower in the SDN group than in the control and sham groups(P<0.05).There were no significant differences in preoperative and postoperative ICP and ICP/mean arterial blood pressure(MAP)among the three groups(P>0.05).Conclusion SDN does not adversely affect the erectile function and sexual desire of rats,and at the same time it can reduce EL and EF,providing an application basis for SDN in the clinical treatment of premature ejaculation. 展开更多
关键词 dorsal penile nerve selective dorsal neurectomy erectile dysfunction premature ejaculation
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Ultrasound-Guided Dorsal Scapular Nerve Blockade in the Diagnosis and Management of Neck Pain
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作者 Alena Boros Paul J. Ryan Dominic C. Harmon 《Pain Studies and Treatment》 2023年第2期9-14,共6页
Neck pain is common and has multiple sources, but correct diagnosis and matched treatment provide the best outcomes. The first description of ultrasound-guided dorsal scapular nerve blockade using a single-shot local ... Neck pain is common and has multiple sources, but correct diagnosis and matched treatment provide the best outcomes. The first description of ultrasound-guided dorsal scapular nerve blockade using a single-shot local anesthetic technique for the diagnosis and treatment of neck pain is reported. A 38-year-old female patient presented with neck pain, and the history and clinical examination strongly suggested myofascial pain affecting the middle scalene muscle. The pain had been unresponsive to pharmacological therapy or physiotherapy. After identifying the dorsal scapular nerve (DSN) in the body of the middle scalene muscle, an ultrasound-guided nerve block was performed using a single injection of local anesthetic to alleviate the patient’s pain. It has been demonstrated that the dorsal scapular nerve can be identified in the neck and effectively blocked using ultrasound guidance. This technique has the potential to assist in the diagnosis and treatment of neck pain originating from the middle scalene muscle. 展开更多
关键词 Technique ULTRASOUND Neck Pain Middle Scalene Muscle dorsal Scapular Nerve
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Unusual capitate fracture with dorsal shearing pattern and concomitant carpometacarpal dislocation with a 6-year follow-up:A case report
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作者 Chien-Cheng Lai Hsu-Wei Fang +3 位作者 Chih-Hung Chang Jwo-Luen Pao Chun-Chien Chang Yeong-Jang Chen 《World Journal of Clinical Cases》 SCIE 2023年第9期2060-2066,共7页
BACKGROUND Isolated capitate fractures are rare carpal fractures.Following high-energy injuries,capitate fractures are usually associated with other carpal fractures or ligament injuries.The management of capitate fra... BACKGROUND Isolated capitate fractures are rare carpal fractures.Following high-energy injuries,capitate fractures are usually associated with other carpal fractures or ligament injuries.The management of capitate fractures depends on the fracture pattern.Here,we report an unusual capitate fracture with a dorsal shearing pattern and concomitant carpometacarpal dislocation,with a 6-year follow-up.To the best of our knowledge,this fracture pattern and surgical management have not been previously reported.CASE SUMMARY A 28-year-old man presented with left-hand volar tenderness and decreased grip strength that persisted for one month after a traffic accident.Radiography showed a distal capitate fracture with carpometacarpal joint incongruence.Computed tomography(CT)revealed a distal capitate fracture with carpometacarpal joint dislocation.The distal fragment was rotated by 90°in the sagittal plane,and an oblique shearing fracture pattern was noted.Open reduction and internal fixation(ORIF)with a locking plate were performed using the dorsal approach.The imaging studies performed 3 mo and 6 years following surgery revealed a healed fracture,and the Disabilities of the Arm,Shoulder,and Hand and visual analog scale scores were significantly improved.CONCLUSION CT can detect capitate fractures with dorsal shearing pattern and concomitant carpometacarpal dislocation.ORIF using a locking plate are possible. 展开更多
关键词 Isolated capitate fracture Carpometacarpal dislocation dorsal intercarpal ligament Case report
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Dorsal approach for isolated volar fracture-dislocation of the base of the second metacarpal:A case report
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作者 Taketo Kurozumi Masato Saito +1 位作者 Kazuhiko Odachi Fumiaki Masui 《World Journal of Clinical Cases》 SCIE 2023年第28期6871-6876,共6页
BACKGROUND We report a case with the displacement of an articular fracture fragment of the base of the second metacarpal from the ulnar to the volar side,treated via the dorsal approach.The dorsal approach can be a go... BACKGROUND We report a case with the displacement of an articular fracture fragment of the base of the second metacarpal from the ulnar to the volar side,treated via the dorsal approach.The dorsal approach can be a good option not only because it allows direct observation of ligament damage and fixation of bone fragments but also because the thin subcutaneous tissue makes the approach easier.CASE SUMMARY A 45-year-old man with a right hand injury visited the hospital.A small bone fragment was identified using plain radiography.Lateral radiography revealed the fragment as lying over the volar aspect of the carpometacarpal(CMC)joint.Computed tomography revealed that approximately one-third of the CMC joint surface of the second metacarpal was damaged.We provisionally diagnosed an intra-articular fracture with significant CMC joint instability and performed open reduction and internal fixation.We made a dorsal longitudinal incision over the CMC joint between the second and third metacarpals.The dorsal ligament of the third CMC joint was torn.We thought it had been dislocated to the volar side and spontaneously reduced to that position.There are only few reports of volar dislocation of CMC joint fractures,particularly of the second and third metacarpals;our report is unique as our patient had an intact interosseous ligament between the second and third metacarpals.CONCLUSION Although past reports have used a palmar approach,the dorsal approach is a good option for these cases. 展开更多
关键词 Metacarpal bone FRACTURE-DISLOCATION Mechanism dorsal approach Carpometacarpal joint Case report
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miR-141-3p对腰椎间盘突出症大鼠背根神经节炎症及下肢疼痛的抑制和改善作用 被引量:5
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作者 许刚 张长春 +2 位作者 朱坤 叶雨辰 周平辉 《中国组织工程研究》 CAS 北大核心 2024年第16期2593-2598,共6页
背景:研究表明,胰岛素样生长因子1/血小板源性生长因子有抑制纤维环细胞凋亡的作用。miR-141-3p微小RNA在骨髓基质细胞中随着年龄的增加而增加,且与炎症信号通路的活化存在一定关系,提示其可能成为腰椎间盘突出症的治疗靶点。目的:探究m... 背景:研究表明,胰岛素样生长因子1/血小板源性生长因子有抑制纤维环细胞凋亡的作用。miR-141-3p微小RNA在骨髓基质细胞中随着年龄的增加而增加,且与炎症信号通路的活化存在一定关系,提示其可能成为腰椎间盘突出症的治疗靶点。目的:探究miR-141-3p通过调控胰岛素样生长因子1/血小板源性生长因子对腰椎间盘突出症大鼠背根神经节炎症及下肢疼痛的影响。方法:选取50只SPF级SD雄性大鼠,随机分为正常组、模型组、miR-NC组、miR-141-3p inhibitor组、miR-141-3p mimics组,每组10只。除正常组外,其余大鼠采用自体髓核移植法进行腰椎间盘突出症建模。建模成功后,对miR-NC组、miR-141-3p inhibitor组和miR-141-3p mimics组大鼠鞘内分别注射10μL 20μmol/L miR-NC,miR-141-3p inhibitor,miR-141-3p mimics,均每天注射1次,连续注射28 d;正常组、模型组同期同位置注射同体积生理盐水。采用热缩足潜伏期阈值评价大鼠下肢疼痛,实时荧光定量PCR检测背根神经节组织miR-141-3p mRNA表达,ELISA法检测背根神经节组织炎症因子,免疫印迹法检测背根神经节组织胰岛素样生长因子1/血小板源性生长因子蛋白表达,并分析miR-141-3p与胰岛素样生长因子1/血小板源性生长因子的相关性。结果与结论:miR-NC组各项指标与模型组比较,差异均无显著性意义。①大鼠热缩足潜伏期阈值:模型组明显低于正常组(P<0.05),miR-141-3p inhibitor组明显低于miR-NC组(P<0.05),miR-141-3p mimics组明显高于miR-141-3p inhibitor组(P<0.05)。②背根神经节组织miR-141-3p mRNA表达:模型组明显低于正常组(P<0.05),miR-141-3p inhibitor组明显低于miR-NC组(P<0.05),miR-141-3p mimics组明显高于miR-141-3p inhibitor组(P<0.05)。③背根神经节组织肿瘤坏死因子α、白细胞介素1β、白细胞介素1含量:模型组明显高于正常组(P<0.05),miR-141-3p inhibitor组明显高于miR-NC组(P<0.05),miR-141-3p mimics组明显低于miR-141-3p inhibitor组(P<0.05)。④背根神经节组织胰岛素样生长因子1、血小板源性生长因子蛋白表达:模型组明显低于正常组(P<0.05),miR-141-3p inhibitor组明显低于miR-NC组(P<0.05),miR-141-3p mimics组明显高于miR-141-3p inhibitor组(P<0.05)。⑤胰岛素样生长因子1与miR-141-3p呈正相关(r=0.904,P<0.001),血小板源性生长因子与miR-141-3p呈正相关(r=0.879,P<0.001)。⑥结论:miR-141-3p可显著改善腰椎间盘突出症大鼠下肢疼痛,抑制背根神经节炎症,其机制可能与促进胰岛素样生长因子1/血小板源性生长因子表达有关。 展开更多
关键词 miR-141-3p IGF-1/PDGF 腰椎间盘突出症 背根神经节炎症 下肢疼痛
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胰淀素作用于背外侧被盖核胆碱乙酰转移酶神经元调节小鼠体重
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作者 曹晓娟 刘昊东 +10 位作者 李鹏辉 李嘉成 樊奇 王星 李彩琴 杨子程 郭永清 陈玉洁 张小宇 海日汗 杜晨光 《畜牧兽医学报》 CAS CSCD 北大核心 2024年第7期3185-3192,共8页
胰淀素(amylin)作用于背外侧被盖核(lateral dorsal tegmental nucleus,LDT),诱导减肥和抑食效应,并激活背肩胛棕色脂肪组织(interscapular brown adipose tissue,IBAT)产热。多种食欲激素受体与胆碱乙酰转移酶(choline acetyltransfera... 胰淀素(amylin)作用于背外侧被盖核(lateral dorsal tegmental nucleus,LDT),诱导减肥和抑食效应,并激活背肩胛棕色脂肪组织(interscapular brown adipose tissue,IBAT)产热。多种食欲激素受体与胆碱乙酰转移酶(choline acetyltransferase,ChAT)神经元共同定位于LDT中,以调节饱腹感和能量消耗。然而,LDT的ChAT神经元是否参与胰淀素介导的体重调节作用尚待探索。作者拟通过免疫荧光检测小鼠LDT中原癌基因蛋白(c-Fos)和ChAT神经元的表达,逆行示踪技术确认LDT的下游神经核团,神经元损伤试验分析胰淀素作用的神经机制。结果显示:腹腔注射胰淀素显著降低小鼠体重,并显著促进LDT和臂旁核(PBN)中c-Fos和ChAT神经元表达。ChAT和神经型一氧化氮合成酶(nNOS)神经元在LDT、PBN和迷走神经背侧核(DMV)中共表达。逆行示踪试验证明LDT向PBN神经元发送神经投射。此外,损伤LDT和PBN神经元可降低胰淀素的减肥效果。LDT是连接PBN的能量平衡调节中枢,ChAT神经元参与介导胰淀素的体重调节过程。 展开更多
关键词 肥胖 胆碱乙酰转移酶 胰淀素 下丘脑 背外侧被盖核
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冷暴露对大鼠痛觉和感觉神经元中瞬时受体电位离子通道的影响
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作者 蒋鼎 曹月龙 +4 位作者 徐勤光 申安平 王楠 邱凤喜 薛艳 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2024年第5期1189-1195,共7页
目的:探讨冷暴露对大鼠痛觉的影响及其对感觉神经元中瞬时受体电位(TRP)离子通道的调控机制,为阐明冷敏感性疼痛的生物学机制提供依据。方法:16只雌性SD大鼠分为对照组(n=8)和寒冷组(n=8)。对照组大鼠置于(24±2)℃环境中,寒冷组大... 目的:探讨冷暴露对大鼠痛觉的影响及其对感觉神经元中瞬时受体电位(TRP)离子通道的调控机制,为阐明冷敏感性疼痛的生物学机制提供依据。方法:16只雌性SD大鼠分为对照组(n=8)和寒冷组(n=8)。对照组大鼠置于(24±2)℃环境中,寒冷组大鼠每日置于人工智能气候室内进行低温(4℃±1℃)刺激4 h,连续1周。采用Von Frey纤维丝检测2组大鼠机械缩足反射阈值(MWT),采用组织免疫荧光染色法观察2组大鼠背根神经节(DRG)组织中TRPA1、TRPM8、TRPV1和TRPV4表达水平,大鼠DRG组织中降钙素基因相关肽(CGRP)和P物质(SP)表达水平以及大鼠滑膜组织中TRPA1、TRPM8、TRPV1和TRPV4表达水平。结果:与对照组比较,寒冷组大鼠MWT明显降低(P<0.05),DRG组织中TRPA1和TRPM8表达水平明显升高(P<0.05),TRPV1表达水平明显降低(P<0.05),TRPV4表达水平差异无统计学意义(P>0.05),CGRP和SP表达水平明显升高(P<0.05)。与对照组比较,寒冷组大鼠滑膜组织中TRPA1表达水平明显升高(P<0.05),TRPM8、TRPV1和TRPV4表达水平明显降低(P<0.05)。结论:短期冷暴露可引起大鼠痛觉过敏,其机制可能与DRG和滑膜组织中TRP离子通道表达变化有关联。TRPA1感觉神经元在关节局部冷痛中起重要作用。 展开更多
关键词 冷暴露 背根神经节 滑膜组织 瞬时受体电位离子通道 痛觉过敏
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电针对紫杉醇诱导大鼠神经病理性疼痛的影响
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作者 欧阳杰 赵海倩 +3 位作者 孔云 牛钦 陈莹 思永玉 《天津医药》 CAS 2024年第11期1141-1146,共6页
目的观察电针(EA)对紫杉醇诱导神经病理性疼痛大鼠脊髓背角NKCC1、KCC2表达和小胶质细胞活化的影响及其可能机制。方法将48只雄性SD大鼠随机分为溶媒组(Vehicle组)、紫杉醇组(PTX组)、紫杉醇+电针组(PTX+EA组)、紫杉醇+假电针组(PTX+Sha... 目的观察电针(EA)对紫杉醇诱导神经病理性疼痛大鼠脊髓背角NKCC1、KCC2表达和小胶质细胞活化的影响及其可能机制。方法将48只雄性SD大鼠随机分为溶媒组(Vehicle组)、紫杉醇组(PTX组)、紫杉醇+电针组(PTX+EA组)、紫杉醇+假电针组(PTX+Sham EA组),每组12只。采用腹腔注射PTX的方法建立PTX诱导的神经病理性疼痛大鼠动物模型,建模完成后,PTX+EA组给予“足三里”、“阳陵泉”电针刺激,连续7 d。于紫杉醇注射前2 d和注射后第1、3、5、7、14、21天进行机械撤足阈值和热缩足潜伏期痛行为学测试。利用免疫荧光染色技术和Western blot技术检测脊髓背角组织中钠钾氯联合转运蛋白1(NKCC1)、钾氯联合转运蛋白2(KCC2)和小胶质细胞标志物离子钙结合衔接分子1(Iba1)表达的变化。结果与Vehicle组比较,PTX组大鼠出现双后足机械和热痛觉过敏,脊髓背角组织中NKCC1表达升高和活化的小胶质细胞数增加。与PTX组比较,PTX+EA组大鼠在第14、21天的机械和热痛觉过敏得到显著改善,脊髓背角组织中NKCC1和Iba1表达量降低,4组间KCC2表达差异无统计学意义。结论电针可有效缓解紫杉醇诱导的神经病理性疼痛,其机制可能与抑制大鼠脊髓背角组织中NKCC1表达和小胶质细胞活化有关。 展开更多
关键词 电针 紫杉醇 神经痛 脊髓背角 小神经胶质细胞 钠钾氯化物协同转运子 神经病理性疼痛
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张振宇精点力学四步手法结合运动康复治疗颈源性肩周痛经验
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作者 冉明山 徐耀 +4 位作者 郑亦斌 徐佳音 范肃 胡芊 张振宇 《北京中医药》 2024年第7期738-741,共4页
颈源性肩周痛是临床常见疾病,因其症状与肩关节周围炎相似,临床易被误诊误治。张振宇教授认为颈5神经背根神经节受刺激后引起肩周不适,从该病理机制入手,利用精点力学四步手法结合康复训练方案治疗本病,能够从根本上解决颈椎及周围结构... 颈源性肩周痛是临床常见疾病,因其症状与肩关节周围炎相似,临床易被误诊误治。张振宇教授认为颈5神经背根神经节受刺激后引起肩周不适,从该病理机制入手,利用精点力学四步手法结合康复训练方案治疗本病,能够从根本上解决颈椎及周围结构退变导致的颈源性肩周疼痛症状及活动功能受限,临床疗效满意。 展开更多
关键词 精点力学手法 颈源性肩周痛 脊背根神经节 临床经验
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指固有动脉背侧支血管链蒂逆行岛状皮瓣修复指端缺损的近期疗效分析及治疗体会
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作者 郑江钒 谷爱奇 +1 位作者 闵祥辉 王煜 《中国实用医药》 2024年第4期60-63,共4页
目的分析指固有动脉背侧支血管链蒂逆行岛状皮瓣修复指端缺损的近期疗效,并总结治疗体会。方法回顾性分析21例(21指)指端缺损患者的临床资料,患者均行指固有动脉背侧支血管链蒂逆行岛状皮瓣修复。随访评估患者的主观满意度、皮瓣感觉恢... 目的分析指固有动脉背侧支血管链蒂逆行岛状皮瓣修复指端缺损的近期疗效,并总结治疗体会。方法回顾性分析21例(21指)指端缺损患者的临床资料,患者均行指固有动脉背侧支血管链蒂逆行岛状皮瓣修复。随访评估患者的主观满意度、皮瓣感觉恢复情况、患指活动功能。结果术后19例患者皮瓣完全成活并顺利拆线,1例因术后私自吸烟致皮瓣严重发绀延迟愈合,1例因术后私自开空调温度过低致皮瓣部分坏死。21例患者中共19例获得随访,随访时间3~18个月,平均(8.9±4.6)个月。获得随访患者中,患者的主观满意度:很满意16例(84.2%)、比较满意3例(15.8%),无不满意病例。皮瓣感觉恢复情况:S415例(78.9%),S3+3例(15.8%),S31例(5.3%)。患指活动功能:优16例(84.2%),良3例(15.8%)。结论指固有动脉背侧支血管链蒂逆行岛状皮瓣修复指端缺损外形功能满意,优势明显,术后积极处理和密切护理对于术后良好疗效亦至关重要。 展开更多
关键词 岛状皮瓣 指固有动脉背侧支 指端缺损 手外伤 创面修复
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指背静脉移植在血管缺损的断指再植术中的临床应用
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作者 于晋辉 田宇 张士伟 《血管与腔内血管外科杂志》 2024年第1期93-97,共5页
目的探讨指背静脉移植在血管缺损的断指再植术中的应用效果。方法收集2021年4月至2022年8月于秦皇岛市第一医院进行断指再植术的35例手指离断患者(40指)的临床资料,术中均采用指背静脉移植桥接术。观察术中镜下挫伤血管内膜挫伤分布情况... 目的探讨指背静脉移植在血管缺损的断指再植术中的应用效果。方法收集2021年4月至2022年8月于秦皇岛市第一医院进行断指再植术的35例手指离断患者(40指)的临床资料,术中均采用指背静脉移植桥接术。观察术中镜下挫伤血管内膜挫伤分布情况,记录血管移植的时间。比较同水平面指背静脉与缺损血管的管径,观察术后再植手指的成活情况、并发症发生情况和成活手指的功能恢复情况。结果术后手指成活率为95.0%(38/40)。自切取静脉至完成两处吻合口的时间一般约为40分钟,吻合质量良好。同水平面指背静脉与缺损血管的管径相当或略粗。术后6个月,在成活的38个再植手指中,手指功能优10指,良25指,差3指,优良率为92.1%(35/38)。结论在血管缺损的断指再植术中,指背静脉移植可在避免再植时过多缩短指骨、关节融合或截指的情况下获得满意的外形和优良的功能。 展开更多
关键词 指背静脉移植 断指再植术 血管缺损
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脊髓背根神经节ZXDC介导CCL2对慢性压迫性 神经损伤小鼠神经性疼痛的作用
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作者 李文媛 王晓宇 +4 位作者 吕忠孝 刘东明 李艺 王淑影 王莹 《医学研究杂志》 2024年第1期56-62,共7页
目的探讨脊髓背根神经节(dorsal root ganglion,DRG)中转录因子锌指X连锁复制C(zinc finger X-linked duplicated C,ZXDC)介导CCL2/CCR2信号通路对慢性压迫性神经损伤(chronic construction injury model,CCI)诱导神经性疼痛的作用及机... 目的探讨脊髓背根神经节(dorsal root ganglion,DRG)中转录因子锌指X连锁复制C(zinc finger X-linked duplicated C,ZXDC)介导CCL2/CCR2信号通路对慢性压迫性神经损伤(chronic construction injury model,CCI)诱导神经性疼痛的作用及机制。方法构建小鼠坐骨神经慢性压迫性损伤模型,免疫荧光染色、Western blot法和实时荧光定量PCR(RT-qPCR)检测正常情况和CCI造模后DRG中ZXDC及CCL2表达变化;将实验动物分为假手术(Sham)组、CCI+AAV-NC组和CCI+AAV-ZXDC siRNA组;Western blot法和免疫荧光染色检测各组小鼠CCI造模后各时间点DRG中ZXDC、CCL2和CCR2表达,RT-qPCR检测DRG中促炎性细胞因子TNF-α和IL-1βmRNA表达,机械性缩足反射测试检测神经性疼痛行为改变。结果ZXDC表达定位在DRG大、中、小神经元。CCI损伤后1~3天DRG中ZXDC和CCL2蛋白和mRNA表达显著增高,CCI后7天二者表达显著降低,ZXDC和CCL2mRNA表达量呈正相关(P均<0.05)。CCI后3天,与Sham组比较,CCI+AAV-NC组和CCI+AAV-ZXDC siRNA组ZXDC、CCL2、CCR2蛋白表达、TNF-α和IL-1βmRNA表达显著增高,其中CCI+AAV-ZXDC siRNA组较CCI+AAV-NC组ZXDC、CCL2、CCR2蛋白表达、TNF-α和IL-1βmRNA显著降低(P均<0.05)。与Sham组比较,CCI+AAV-ZXDC siRNA组和CCI+AAV-NC组CCI后各时间点机械缩足阈值均显著降低,其中CCI后7天,CCI+AAV-ZXDC siRNA组机械缩足阈值显著高于CCI+AAV-NC组(P均<0.05)。结论脊髓背根神经节ZXDC基因敲减通过抑制CCL2/CCR2信号通路介导CCI诱导的神经性疼痛。 展开更多
关键词 脊髓背根神经节 锌指X连锁复制C CC趋化因子2 神经损伤 慢性神经疼痛
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血清5-HT、NGF水平与原发性早泄患者阴茎背神经选择性切除术后复发的关系
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作者 李鸿斌 侯琳 +2 位作者 邢建东 冯东 樊茂宇 《山东医药》 CAS 2024年第19期30-34,共5页
目的探究血清5-羟色胺(5-HT)、神经生长因子(NGF)水平变化与原发性早泄患者阴茎背神经选择性切除术后复发的关系。方法选择原发性早泄患者200例,均行阴茎背神经选择性切除术治疗,术后6个月根据患者是否复发分为复发组(n=23)与未复发组(n... 目的探究血清5-羟色胺(5-HT)、神经生长因子(NGF)水平变化与原发性早泄患者阴茎背神经选择性切除术后复发的关系。方法选择原发性早泄患者200例,均行阴茎背神经选择性切除术治疗,术后6个月根据患者是否复发分为复发组(n=23)与未复发组(n=177),比较两组患者的临床资料、血清5-HT、NGF水平,分析5-HT、NGF与中国早泄问卷调查表(CIPE-5)、国际勃起功能(IIEF-5)评分、射精潜伏期(IELT)的相关性及术后复发影响因素,并构建列线图模型评价含血清指标模型预测术后复发与实际观察的一致性,用决策曲线评价该模型的临床获益。结果复发组术后3个月血清5-HT水平、IELT、CIPE-5评分均低于未复发组,NGF水平高于未复发组(P均<0.05);原发性早泄患者术前、术后1个月、术后3个月,血清5-HT水平与CIPE-5评分、IELT呈正相关(P均<0.05),与IIEF-5评分无相关性(P均>0.05);血清NGF水平与CIPE-5评分、IELT呈负相关(P均<0.05),与IIEF-5评分无相关性(P均>0.05);术后3个月IELT、CIPE-5评分及血清5-HT、NGF水平均为原发性早泄患者术后复发的独立影响因素(P均<0.05);列线图模型预测原发性早泄患者术后复发相关因素的一致性指数为0.775(95%CI=0.674~0.802);决策曲线显示,当含血清指标的模型预测原发性早泄患者术后复发的值为0.20~0.85时,可提供附加临床获益。结论原发性早泄患者阴茎背神经选择性切除术后3个月血清5-HT水平下降、NGF水平升高,二者水平变化对预测患者术后复发有一定参考价值。 展开更多
关键词 原发性早泄 阴茎背神经选择性切除术 复发 5-羟色胺 神经生长因子
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专业训练中篮球鞋对下肢关节的运动生物力学影响研究综述
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作者 宗延伟 段伟 《中国皮革》 CAS 2024年第9期91-94,共4页
篮球运动员在专业训练时会进行各式各样的技术动作,尤其是急停起跳、迅速移动等,需时刻转变步伐,会明显增加下肢关节负荷,这就需要运动员穿着合适的运动鞋,以确保其稳定性可以充分支撑与保护足部而避免下肢关节损伤。据此,为保障运动员... 篮球运动员在专业训练时会进行各式各样的技术动作,尤其是急停起跳、迅速移动等,需时刻转变步伐,会明显增加下肢关节负荷,这就需要运动员穿着合适的运动鞋,以确保其稳定性可以充分支撑与保护足部而避免下肢关节损伤。据此,为保障运动员在篮球训练及比赛中的安全性与技术发挥实效性,本文全面综述了专业运动训练中篮球鞋对下肢关节的运动生物力学影响研究成果,并进一步做了相关研究展望,以期能够为篮球鞋整体结构优化设计,有效应对运动员下肢关节损伤提供有利帮助。 展开更多
关键词 专业运动训练 篮球鞋 踝关节 背屈角 生物力学
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复元止痛方辅助腰背肌功能训练对腰椎术后慢性腰痛患者疼痛缓解和腰椎功能康复的影响
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作者 史黎 史凌云 +1 位作者 郭晓斌 张丽 《吉林中医药》 2024年第8期932-935,共4页
目的探讨复元止痛方辅助腰背肌功能训练对腰椎术后慢性腰痛患者疼痛缓解和腰椎功能康复的影响。方法选取2021年1月-2023年1月收治的腰椎术后慢性腰痛患者86例,分为对照组和观察组,各43例。对照组患者采用塞来昔布胶囊口服+腰背肌功能训... 目的探讨复元止痛方辅助腰背肌功能训练对腰椎术后慢性腰痛患者疼痛缓解和腰椎功能康复的影响。方法选取2021年1月-2023年1月收治的腰椎术后慢性腰痛患者86例,分为对照组和观察组,各43例。对照组患者采用塞来昔布胶囊口服+腰背肌功能训练治疗,观察组患者在对照组治疗基础上联合复元止痛方治疗,连续治疗4周。观察2组患者治疗前后中医症状积分、VAS和ODI评分、血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、白介素-6(interleukin-6,IL-6)、C反应蛋白(C-reactive protein,CRP)]水平改善情况,并比较2组治疗后总有效率及治疗方案的安全性。结果治疗后,2组腰痛如针刺、疼痛拒按、腰椎活动受限、舌质紫黯积分,VAS评分,ODI评分均明显降低(P<0.05),且观察组治疗后中医症状积分及量表评分均低于对照组(P<0.05);2组TNF-α、IL-6、CRP均显著下降(P<0.05),观察组低于对照组(P<0.05);观察组总有效率93.02%明显高于对照组76.74%(P<0.05);观察组药物不良反应总发生率4.65%明显低于对照组23.26%(P<0.05)。结论采用复元止痛方配合腰背肌功能训练治疗腰椎术后慢性腰痛,能够有效减轻疼痛程度,降低炎性因子水平,促进腰椎功能恢复和临床症状缓解,且治疗安全性较高。 展开更多
关键词 腰椎内固定术 慢性腰痛 复元止痛方 腰背肌功能训练 疼痛 腰椎功能 炎性因子
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