Spinal cord injury has devastating consequences because adult central nervous system （CNS） neurons do not regenerate their axons after injury. Two key reasons for axon regeneration fail- ure are extrinsic inhibitory...Spinal cord injury has devastating consequences because adult central nervous system （CNS） neurons do not regenerate their axons after injury. Two key reasons for axon regeneration fail- ure are extrinsic inhibitory factors and a low intrinsic capacity for axon regrowth. Research has therefore focused on overcom- ing extrinsic growth inhibition, and enhancing intrinsic regeneration capacity. Both of these issues will need to be addressed to enable optimal repair of the injured sp＋inal cord.展开更多
To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is proposed.Differen...To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is proposed.Different from the traditional fault diagnosis optimization algorithms,the fault intelligent learning method pro-posed in this paper is able to quickly identify the faults of inter-satellite link control system despite the existence of strong cou-pling nonlinearity.By constructing a two-layer learning network,the method enables efficient joint diagnosis of fault areas and fault parameters.The simulation results show that the average identification time of the system fault area and fault parameters is 0.27 s,and the fault diagnosis efficiency is improved by 99.8%compared with the traditional algorithm.展开更多
AIM： To compare three kinds of fluorescent probes for in vitro labeling and in vivo tracking of endothelial progenitor cells（EPCs） in a mouse model of laser-induced retinal injury.METHODS： EPCs were isolated from ...AIM： To compare three kinds of fluorescent probes for in vitro labeling and in vivo tracking of endothelial progenitor cells（EPCs） in a mouse model of laser-induced retinal injury.METHODS： EPCs were isolated from human umbilical cord blood mononuclear cells and labeled with three different fluorescent probes： 5-（and-6）-carboxyfluorescein diacetate succinimidyl ester（CFSE）, 1,1′-dilinoleyl-3,3,3′,3′-tetramethylindo-carbocyanine perchlorate linked acetylated low-density lipoprotein（Di I-Ac LDL）, and green fluorescent protein（GFP）. The fluorescent intensity of EPCs was examined by confocal microscopy. Survival rate of labeled EPCs was calculated with trypan blue staining, and their adhesive capability was assessed. A mouse model of retinal injury was induced by laser, and EPCs were injected into the vitreous cavity. Frozen section and fluorescein angiography on flat-mounted retinal samples was employed to track the labeled EPCs in vivo.RESULTS： EPCs labeled with CFSE and Di I-Ac LDL exhibited an intense green and red fluorescence at the beginning; the fluorescence intensity decreased gradually to 20.23% and 49.99% respectively, after 28 d. On the contrary, the florescent intensity of GFP-labeled EPCs increased in a time-dependent manner. All labeled EPCs showed normal morphology and no significant change in survival and adhesive capability. In the mouse model, transplantation of EPCs showed a protective effect against retinal injury. EPCs labeled with CFSE and Di I-Ac LDL were successfully tracked in mice during the development of retinal injury and repair; however, GFP-labeled EPCs were not detected in the laser-injured mouse retina.CONCLUSION： The three fluorescent markers used in this study have their own set of advantages and disadvantages. CFSE and Di I-Ac LDL are suitable for short-term EPClabeling, while GFP should be used for long-term labeling. The choice of fluorescent markers should be guided by the purpose of the study.展开更多
The divergence angle of laser beam used in space laser communication is usually no more than 100μrad.Using laser beam with small divergence angle to achieve acquisition and tracking for space laser link has always be...The divergence angle of laser beam used in space laser communication is usually no more than 100μrad.Using laser beam with small divergence angle to achieve acquisition and tracking for space laser link has always been a difficult problem.In addition,the random nature of the atmosphere will affect the satellite-ground laser link,which increases the difficulty of the acquisition and stable tracking for the laser link.Thus,taking into account the above challenges for satellite-ground laser communication,an acquisition and tracking scheme of using both beacon beam and signal beam was designed for the Laser Communication Terminal(LCT)of Shijian 20 satellite.In-orbit test results indicated that under the condition of moderate atmospheric turbulence(atmospheric coherence length r0≈3 cm),the process of acquisition and tracking for the satellite-ground laser link can be completed within 1 s after the initial pointing between the LCT and Optical Ground Station(OGS)is performed,and the tracking error was less than 1μrad(3σ).In addition,the laser link can be re-established quickly once being interrupted by unsteady atmospheric turbulence,and can be maintained for a long time under moderate twurbulence conditions,which lays a foundation for future application of satellite-ground laser communication.展开更多
基金funded by grants from the Christopher and Dana Reeve Foundation[JFC-2013(3),JFC-2013(4)]the Medical Research Council(G1000864 018556)+1 种基金the International Spinal Research Trust(Nathalie Rose Barr studentship NRB110)ERANET NEURON grant Axon Repair(013-16-002)
文摘Spinal cord injury has devastating consequences because adult central nervous system （CNS） neurons do not regenerate their axons after injury. Two key reasons for axon regeneration fail- ure are extrinsic inhibitory factors and a low intrinsic capacity for axon regrowth. Research has therefore focused on overcom- ing extrinsic growth inhibition, and enhancing intrinsic regeneration capacity. Both of these issues will need to be addressed to enable optimal repair of the injured sp＋inal cord.
基金This work was supported by the National Key Research and Development Program Topics(2020YFC2200902)the National Natural Science Foundation of China(11872110).
文摘To maintain the stability of the inter-satellite link for gravitational wave detection,an intelligent learning monitoring and fast warning method of the inter-satellite link control system failure is proposed.Different from the traditional fault diagnosis optimization algorithms,the fault intelligent learning method pro-posed in this paper is able to quickly identify the faults of inter-satellite link control system despite the existence of strong cou-pling nonlinearity.By constructing a two-layer learning network,the method enables efficient joint diagnosis of fault areas and fault parameters.The simulation results show that the average identification time of the system fault area and fault parameters is 0.27 s,and the fault diagnosis efficiency is improved by 99.8%compared with the traditional algorithm.
基金Supported by the National Natural Science Foundation of China(No.81400403)the International Science and Technology Cooperation Program of Jilin Province(No.20110733)the Technology Program of Soochow City(No.SYS201375)
文摘AIM： To compare three kinds of fluorescent probes for in vitro labeling and in vivo tracking of endothelial progenitor cells（EPCs） in a mouse model of laser-induced retinal injury.METHODS： EPCs were isolated from human umbilical cord blood mononuclear cells and labeled with three different fluorescent probes： 5-（and-6）-carboxyfluorescein diacetate succinimidyl ester（CFSE）, 1,1′-dilinoleyl-3,3,3′,3′-tetramethylindo-carbocyanine perchlorate linked acetylated low-density lipoprotein（Di I-Ac LDL）, and green fluorescent protein（GFP）. The fluorescent intensity of EPCs was examined by confocal microscopy. Survival rate of labeled EPCs was calculated with trypan blue staining, and their adhesive capability was assessed. A mouse model of retinal injury was induced by laser, and EPCs were injected into the vitreous cavity. Frozen section and fluorescein angiography on flat-mounted retinal samples was employed to track the labeled EPCs in vivo.RESULTS： EPCs labeled with CFSE and Di I-Ac LDL exhibited an intense green and red fluorescence at the beginning; the fluorescence intensity decreased gradually to 20.23% and 49.99% respectively, after 28 d. On the contrary, the florescent intensity of GFP-labeled EPCs increased in a time-dependent manner. All labeled EPCs showed normal morphology and no significant change in survival and adhesive capability. In the mouse model, transplantation of EPCs showed a protective effect against retinal injury. EPCs labeled with CFSE and Di I-Ac LDL were successfully tracked in mice during the development of retinal injury and repair; however, GFP-labeled EPCs were not detected in the laser-injured mouse retina.CONCLUSION： The three fluorescent markers used in this study have their own set of advantages and disadvantages. CFSE and Di I-Ac LDL are suitable for short-term EPClabeling, while GFP should be used for long-term labeling. The choice of fluorescent markers should be guided by the purpose of the study.
文摘The divergence angle of laser beam used in space laser communication is usually no more than 100μrad.Using laser beam with small divergence angle to achieve acquisition and tracking for space laser link has always been a difficult problem.In addition,the random nature of the atmosphere will affect the satellite-ground laser link,which increases the difficulty of the acquisition and stable tracking for the laser link.Thus,taking into account the above challenges for satellite-ground laser communication,an acquisition and tracking scheme of using both beacon beam and signal beam was designed for the Laser Communication Terminal(LCT)of Shijian 20 satellite.In-orbit test results indicated that under the condition of moderate atmospheric turbulence(atmospheric coherence length r0≈3 cm),the process of acquisition and tracking for the satellite-ground laser link can be completed within 1 s after the initial pointing between the LCT and Optical Ground Station(OGS)is performed,and the tracking error was less than 1μrad(3σ).In addition,the laser link can be re-established quickly once being interrupted by unsteady atmospheric turbulence,and can be maintained for a long time under moderate twurbulence conditions,which lays a foundation for future application of satellite-ground laser communication.
